pycom17g15950

guanosine tetraphosphate biosynthetic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
N/A
Physical Location & Seq
Forward (+)
14271809 .. 14273424
1616 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 942 bp
ATGACGGTCCCGACAATAGCTCTGTACGCGGCGCACCCGTGCCAGATCAACGCGCACACCTCACACGATTTCGAATTGGGCTCTCGATCTTCTTCGTCAACGGCGTCGACCCCCTCCACTTCGCAGAAGCCGGTGACTGGCGGGCTCTCGTGCCTATTCTCCTCGCCGACGGTGAAGCATGCGTCTTCCTCGTCGAGTTTTTCGGGCGGAGGAGAGGAATTGGGCTCTCTATGGCACGATAGAGGCGAGGAATTGAGCAGCTCGTTTCGGTATTCTGGGAGCAAGTTCAATGGGGCTTCGATGAACCGGGACCAGAGCCCGGTTTCAGTGTTTCAGGGTCCGGTTTCGTCTTCCAGTAGCGGTGTTTCAGGGTCGGCGAGGAGCCCACCAATGAGAATTACAAGGGAAAGGTCTGGCAATGGAGATATTAGCTTGAATTCGATTCGGTGTAGAAGTAATGGGTTGTTTAATGGGTTTGTGAGGGGTGCTTTGGGCTCTTCCTGCATAGATTACGACTCGCCGAGCTTCGAGGTTCAGACTGATGGTTTGGATGTGGGTTCATCAGCTGTGCTTGTTGATGAATTGACTTTCAACATGGAAGATGGGTTTTTGGAGGGAATTGCTGAGCCTTATGCTAAGGAGTTGCTTGTGGGTGCGCAGCTGAGGCACAAGATTTTCTATGAAGATTTTATTATCAAGGCCTTTTACGAGGCTGAGAAAGCGCATAGAGGGCAGATGCGAGCAAGTGGCGGTCCTTATTTGCAGCATTGTGTGGAGACAGCGGTGTTGCTCGCATTGATCGGTTCTAATTCGACAGTTGTTGCTGCAGGGCTTTTGCATGACACGCTTGATGATTCTTTTATGTGTTATGACTACATATTTGGGAAATTTGGAGCCGGGTTCTTTATGGAATTTATTAGGTGTCTAAGCTCAGTCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008728 GO:0008893 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009150 GO:0009152 GO:0009163 GO:0009165 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009605 GO:0009611 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0009991 GO:0010033 GO:0010150 GO:0015969 GO:0015970 GO:0015979 GO:0016020 GO:0016740 GO:0016772 GO:0016778 GO:0016787 GO:0016788 GO:0016794 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0030312 GO:0031667 GO:0032501 GO:0032502 GO:0033865 GO:0033866 GO:0033875 GO:0033993 GO:0034030 GO:0034032 GO:0034033 GO:0034035 GO:0034036 GO:0034404 GO:0034641 GO:0034654 GO:0042221 GO:0042278 GO:0042451 GO:0042455 GO:0042578 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046128 GO:0046129 GO:0046390 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0055086 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:0090693 GO:0097305 GO:0099402 GO:1901068 GO:1901070 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00230 map00230
Pfam Domains
Protein Families
HD_4 RelA_SpoT

Protein Analysis

314

Amino Acids

33.44

Weight (kDa)

5.46

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD_4 PF13328 234 - 286 8.1e-08 HD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 657
AccI GTMKAC 1 cut(s) 107
AccII CGCG 2 cut(s) 29, 53
AciI CCGC 6 cut(s) 29, 141, 207, 360, 750, 782
AcsI RAATTY 3 cut(s) 436, 887, 911
AcyI GRCGYC 1 cut(s) 104
AfaI GTAC 1 cut(s) 26
AfiI CCNNNNNNNGG 2 cut(s) 137, 319
AgsI TTSAA 3 cut(s) 289, 436, 592
AluBI AGCT 7 cut(s) 20, 261, 432, 525, 566, 661, 930
AluI AGCT 7 cut(s) 20, 261, 432, 525, 566, 661, 930
Alw26I GTCTC 1 cut(s) 770
AoxI GGCC 1 cut(s) 699
ApeKI GCWGC 4 cut(s) 258, 658, 763, 824
ApoI RAATTY 3 cut(s) 436, 887, 911
ArsI GACNNNNNNTTYG 4 cut(s) 529, 561, 863, 895
AspLEI GCGC 4 cut(s) 34, 55, 658, 724
AspS9I GGNCC 4 cut(s) 7, 310, 338, 752
AsuC2I CCSGG 3 cut(s) 308, 320, 898
AsuHPI GGTGA 2 cut(s) 145, 184
AsuII TTCGAA 1 cut(s) 72
AvaII GGWCC 4 cut(s) 7, 310, 338, 752
BanII GRGCYC 6 cut(s) 83, 147, 227, 320, 386, 497
BauI CACGAG 1 cut(s) 148
BbsI GAAGAC 2 cut(s) 177, 342
BbvCI CCTCAGC 1 cut(s) 662
BbvI GCAGC 4 cut(s) 270, 670, 775, 811
BccI CCATC 2 cut(s) 536, 596
BceAI ACGGC 1 cut(s) 117
BcnI CCSGG 3 cut(s) 308, 320, 898
BcoDI GTCTC 1 cut(s) 770
BfmI CTRYAG 1 cut(s) 825
BisI GCNGC 5 cut(s) 30, 259, 659, 764, 825
BlpI GCTNAGC 1 cut(s) 624
BlsI GCNGC 5 cut(s) 31, 260, 660, 765, 826
Bme1390I CCNGG 3 cut(s) 308, 320, 898
Bme18I GGWCC 4 cut(s) 7, 310, 338, 752
BmgT120I GGNCC 4 cut(s) 7, 310, 338, 752
BmiI GGNNCC 5 cut(s) 9, 311, 339, 383, 895
BmrFI CCNGG 3 cut(s) 308, 320, 898
BmsI GCATC 1 cut(s) 726
BpiI GAAGAC 2 cut(s) 177, 342
Bpu10I CCTNAGC 2 cut(s) 636, 662
Bpu1102I GCTNAGC 1 cut(s) 624
Bpu14I TTCGAA 1 cut(s) 72
BpuMI CCSGG 3 cut(s) 308, 320, 898
BsaHI GRCGYC 1 cut(s) 104
BsaWI WCCGGW 1 cut(s) 340
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 319
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 2 cut(s) 142, 354
Bse3DI GCAATG 1 cut(s) 424
BseGI GGATG 1 cut(s) 556
BseLI CCNNNNNNNGG 2 cut(s) 137, 319
BseMI GCAATG 1 cut(s) 424
BseMII CTCAG 3 cut(s) 615, 653, 705
BseNI ACTGG 2 cut(s) 142, 354
BseRI GAGGAG 3 cut(s) 151, 225, 394
BseXI GCAGC 4 cut(s) 270, 670, 775, 811
Bsh1236I CGCG 2 cut(s) 29, 53
BshFI GGCC 1 cut(s) 701
BsiSI CCGG 5 cut(s) 131, 307, 320, 341, 897
BslFI GGGAC 1 cut(s) 323
BslI CCNNNNNNNGG 2 cut(s) 137, 319
BsmAI GTCTC 1 cut(s) 770
BsmFI GGGAC 1 cut(s) 323
BsnI GGCC 1 cut(s) 701
Bsp119I TTCGAA 1 cut(s) 72
Bsp1286I GDGCHC 6 cut(s) 83, 147, 227, 320, 386, 497
Bsp143I GATC 3 cut(s) 45, 86, 798
Bsp1720I GCTNAGC 1 cut(s) 624
BspACI CCGC 6 cut(s) 29, 141, 207, 360, 750, 782
BspANI GGCC 1 cut(s) 701
BspCNI CTCAG 3 cut(s) 616, 654, 706
BspFNI CGCG 2 cut(s) 29, 53
BspLI GGNNCC 5 cut(s) 9, 311, 339, 383, 895
BspMAI CTGCAG 1 cut(s) 829
BspQI GCTCTTC 1 cut(s) 502
BspT104I TTCGAA 1 cut(s) 72
BsrDI GCAATG 1 cut(s) 424
BsrFI RCCGGY 1 cut(s) 130
BsrI ACTGG 2 cut(s) 142, 354
BssAI RCCGGY 1 cut(s) 130
BssMI GATC 3 cut(s) 45, 86, 798
BssNI GRCGYC 1 cut(s) 104
BssSI CACGAG 1 cut(s) 148
Bst2BI CACGAG 1 cut(s) 148
Bst4CI ACNGT 3 cut(s) 7, 172, 817
Bst6I CTCTTC 1 cut(s) 502
BstACI GRCGYC 1 cut(s) 104
BstBI TTCGAA 1 cut(s) 72
BstC8I GCNNGC 4 cut(s) 143, 180, 741, 792
BstDEI CTNAG 6 cut(s) 624, 636, 662, 714, 926, 931
BstF5I GGATG 1 cut(s) 556
BstFNI CGCG 2 cut(s) 29, 53
BstHHI GCGC 4 cut(s) 34, 55, 658, 724
BstKTI GATC 3 cut(s) 48, 89, 801
BstMAI GTCTC 1 cut(s) 770
BstMBI GATC 3 cut(s) 45, 86, 798
BstMWI GCNNNNNNNGC 6 cut(s) 26, 501, 664, 719, 730, 844
BstNSI RCATGY 1 cut(s) 182
BstSCI CCNGG 3 cut(s) 306, 318, 896
BstSFI CTRYAG 1 cut(s) 825
BstUI CGCG 2 cut(s) 29, 53
BstV1I GCAGC 4 cut(s) 270, 670, 775, 811
BstV2I GAAGAC 2 cut(s) 177, 342
BsuRI GGCC 1 cut(s) 701
BtsCI GGATG 1 cut(s) 556
BtsIMutI CAGTG 1 cut(s) 333
Cac8I GCNNGC 4 cut(s) 143, 180, 741, 792
CfoI GCGC 4 cut(s) 34, 55, 658, 724
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 4 cut(s) 7, 310, 338, 752
CseI GACGC 2 cut(s) 93, 171
Csp6I GTAC 1 cut(s) 25
CviAII CATG 3 cut(s) 179, 595, 839
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 6 cut(s) 624, 636, 662, 714, 926, 931
DpnI GATC 3 cut(s) 47, 88, 800
DpnII GATC 3 cut(s) 45, 86, 798
Eam1104I CTCTTC 1 cut(s) 502
EarI CTCTTC 1 cut(s) 502
EciI GGCGGA 1 cut(s) 222
Eco147I AGGCCT 1 cut(s) 701
Eco24I GRGCYC 6 cut(s) 83, 147, 227, 320, 386, 497
Eco47I GGWCC 4 cut(s) 7, 310, 338, 752
EcoRI GAATTC 1 cut(s) 436
EcoT38I GRGCYC 6 cut(s) 83, 147, 227, 320, 386, 497
FaeI CATG 3 cut(s) 182, 598, 842
FaqI GGGAC 1 cut(s) 323
FatI CATG 3 cut(s) 178, 594, 838
FauI CCCGC 1 cut(s) 134
FblI GTMKAC 1 cut(s) 107
Fnu4HI GCNGC 5 cut(s) 30, 259, 659, 764, 825
FokI GGATG 1 cut(s) 563
FriOI GRGCYC 6 cut(s) 83, 147, 227, 320, 386, 497
Fsp4HI GCNGC 5 cut(s) 30, 259, 659, 764, 825
FspI TGCGCA 1 cut(s) 657
GlaI GCGC 4 cut(s) 33, 54, 657, 723
GluI GCNGC 5 cut(s) 30, 259, 659, 764, 825
HaeIII GGCC 1 cut(s) 701
HapII CCGG 5 cut(s) 131, 307, 320, 341, 897
HgaI GACGC 2 cut(s) 93, 171
HhaI GCGC 4 cut(s) 34, 55, 658, 724
Hin1I GRCGYC 1 cut(s) 104
Hin1II CATG 3 cut(s) 182, 598, 842
Hin6I GCGC 4 cut(s) 32, 53, 656, 722
HinP1I GCGC 4 cut(s) 32, 53, 656, 722
HincII GTYRAC 2 cut(s) 99, 108
HindII GTYRAC 2 cut(s) 99, 108
HinfI GANTC 3 cut(s) 442, 515, 854
HpaII CCGG 5 cut(s) 131, 307, 320, 341, 897
HphI GGTGA 2 cut(s) 145, 184
Hpy166II GTNNAC 2 cut(s) 99, 108
Hpy188I TCNGA 1 cut(s) 537
Hpy188III TCNNGA 2 cut(s) 10, 84
Hpy8I GTNNAC 2 cut(s) 99, 108
Hpy99I CGWCG 3 cut(s) 109, 172, 196
HpyCH4III ACNGT 3 cut(s) 7, 172, 817
HpyCH4V TGCA 4 cut(s) 504, 763, 827, 838
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 501, 664, 719, 730, 844
HpyF3I CTNAG 6 cut(s) 624, 636, 662, 714, 926, 931
Hsp92I GRCGYC 1 cut(s) 104
Hsp92II CATG 3 cut(s) 182, 598, 842
HspAI GCGC 4 cut(s) 32, 53, 656, 722
Kzo9I GATC 3 cut(s) 45, 86, 798
LguI GCTCTTC 1 cut(s) 502
LmnI GCTCC 3 cut(s) 279, 381, 893
Lsp1109I GCAGC 4 cut(s) 270, 670, 775, 811
LweI GCATC 1 cut(s) 726
MaeIII GTNAC 1 cut(s) 133
MalI GATC 3 cut(s) 47, 88, 800
MboI GATC 3 cut(s) 45, 86, 798
MboII GAAGA 7 cut(s) 81, 84, 177, 342, 489, 611, 695
MhlI GDGCHC 6 cut(s) 83, 147, 227, 320, 386, 497
MlyI GAGTC 1 cut(s) 509
MseI TTAA 1 cut(s) 468
MspA1I CMGCKG 3 cut(s) 566, 661, 782
MspI CCGG 5 cut(s) 131, 307, 320, 341, 897
MspR9I CCNGG 3 cut(s) 308, 320, 898
MvnI CGCG 2 cut(s) 29, 53
MwoI GCNNNNNNNGC 6 cut(s) 26, 501, 664, 719, 730, 844
NciI CCSGG 3 cut(s) 308, 320, 898
NdeII GATC 3 cut(s) 45, 86, 798
NlaIII CATG 3 cut(s) 182, 598, 842
NlaIV GGNNCC 5 cut(s) 9, 311, 339, 383, 895
NmeAIII GCCGAG 1 cut(s) 546
NmuCI GTSAC 1 cut(s) 133
NsbI TGCGCA 1 cut(s) 657
NspI RCATGY 1 cut(s) 182
NspV TTCGAA 1 cut(s) 72
PaeI GCATGC 1 cut(s) 182
PceI AGGCCT 1 cut(s) 701
PciSI GCTCTTC 1 cut(s) 502
PcsI WCGNNNNNNNCGW 2 cut(s) 101, 243
PfeI GAWTC 2 cut(s) 442, 854
PkrI GCNGC 5 cut(s) 31, 260, 660, 765, 826
PleI GAGTC 1 cut(s) 509
PpsI GAGTC 1 cut(s) 509
PspN4I GGNNCC 5 cut(s) 9, 311, 339, 383, 895
PspPI GGNCC 4 cut(s) 7, 310, 338, 752
PstI CTGCAG 1 cut(s) 829
PvuII CAGCTG 2 cut(s) 566, 661
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
SalI GTCGAC 1 cut(s) 106
SapI GCTCTTC 1 cut(s) 502
SaqAI TTAA 1 cut(s) 468
SatI GCNGC 5 cut(s) 30, 259, 659, 764, 825
Sau3AI GATC 3 cut(s) 45, 86, 798
Sau96I GGNCC 4 cut(s) 7, 310, 338, 752
SchI GAGTC 1 cut(s) 509
ScrFI CCNGG 3 cut(s) 308, 320, 898
SduI GDGCHC 6 cut(s) 83, 147, 227, 320, 386, 497
SfaNI GCATC 1 cut(s) 726
SfcI CTRYAG 1 cut(s) 825
SfuI TTCGAA 1 cut(s) 72
SinI GGWCC 4 cut(s) 7, 310, 338, 752
SphI GCATGC 1 cut(s) 182
SseBI AGGCCT 1 cut(s) 701
SsiI CCGC 6 cut(s) 29, 141, 207, 360, 750, 782
StuI AGGCCT 1 cut(s) 701
StyD4I CCNGG 3 cut(s) 306, 318, 896
TaaI ACNGT 3 cut(s) 7, 172, 817
TaqI TCGA 8 cut(s) 72, 85, 107, 194, 299, 440, 528, 812
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 2 cut(s) 442, 854
Tru1I TTAA 1 cut(s) 468
Tru9I TTAA 1 cut(s) 468
TscAI CASTG 1 cut(s) 333
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 4 cut(s) 258, 658, 763, 824
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 4 cut(s) 317, 549, 594, 696
TspRI CASTG 1 cut(s) 333
VpaK11BI GGWCC 4 cut(s) 7, 310, 338, 752
XapI RAATTY 3 cut(s) 436, 887, 911
XceI RCATGY 1 cut(s) 182
XmiI GTMKAC 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.