pycom17g21360

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
N/A
Physical Location & Seq
Forward (+)
19740098 .. 19743304
3207 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3207 bp
ATGAAATCTCTTAAATACCTTTATGTTTCTAGATGCTCAAAGCTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGAAGAATCTATCTGAGCTTTATTTGGATGGCACTGCAATTAAGGAGCTGCCTGCTTCTATTTTAAATCTCACCAGCCTTGTTACTTTGAAGCTGAATTATTGCAGAGAACTTGAAAGTCTTCCAAGCAGCATTTCTCACATGAAATCTCTTGAATACCTTGATGTTTCTGGATGCTCAAAGCTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGCAGAAGATATCTGGGCTTTATTTGGGTGGCACTGCAATTAAGGAGTTGCCTGCCTTTATTTTAAATCTCACCAGTCTTGTTACTTTGAAGTTGGTTGATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATTTCTCACATGAAATCTCTGAAATACCTTTATGTTTATGGATGCTCAAAGCTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGAAGAATCTATCTGAGCTTGATTTGGGTGGCACTGCAATTAAGGAGCTGCCTGCTTCTATTTTAAATCTCACCAGTCTTGTTACTTTGAAGCTGAATGATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATTTCTCACATGAAATCTCTTGAATACCTTTATGTTTCTGGATGCTCAAAGCTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGAAGAATCTATCTGAGCTTGATTTGGGTGGCACTGCAATTAAGGAGCTGCTTGCTTCTATTTTAAATCTCACCAGTCTTGTTACTTTGAAGCTGAATGATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATTTCTCACATGAAATCTCTTAAATACCTTGATGTTTCTGGATGCTCAAAGCTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGAATAATCTCTTTAAGCTTTATTTAGGTGGCACTGCAATTAAGGAATTGCCTCCGTCAGTTTTAAATCTCATCAGTCTTGTAACTTTGAAGTTGAATGGTTGTAGAGAACTTGAGAGTCTTCCAAGCACCATTTGTCATATGAAATCTCTTCAATACCTTGATGTTTCTGGATGCTCAAAGCTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGGATAATCTATTTGAGCTTTATTTGGATGGCACTGCAATTAAGGAGCTGCCTGCTTCTATTTTAAATCTCACCAGTCTTGTTACTTTGAAGCTGAATGATTGCAGAGAACTTAAGAGTCTTCCAAGCATCATTTCTCACATGAAATCTCTTAAATACCTTCTTGTTTTTGGACTCTCAAAGCTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGAATAATCTATTTGGGCTTTATTTGGTTGACATTGCAATTAAGGAGCTGCCTGCTTCTATTTTAAATCTCACCAGTCTTGTTACTTTGAAGCTGAATGATTGCAGAAAACTTGAGAGTCTTCCAAGCAGCATTTCTCACATGAAATCTCTTAAATACCTTTATGTTTCTGGATGCTCAAAGGTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGAATAATCTATTTGACCTTTATTTGGATGGCACTGCAATTAAGGAGATGCCTGCTTCTATTTTAAATCTCACCAGTCTTGTTAATTTGAAGCTGAATGATTGCAGACAACTTGAGAGTCTTCCAAGCAGCATTTCTCACATGAAATCTCTTAAATTCCTTTATGTTTCTGGATGCTCAAAGCTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGAATAATCTATTTGGACTTTATTTGGATGGCACTGCAATTAAGGAGCTACATGCTTCTATTTTAAATCTCACCAGTCTTGTTACTTTGAAGTTGAATGATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATTTCTCACGTGAAATCTCTTAAATACCTTTATGTTTCTGGATGCTCAAAGCTTGAGAAGTTTCCAGAAATTTTAGAGGTTATGAATAATCTATTTGCGCTTTATTTGGATGGCACTGCAATTAAGGAGCTACCTGCTTCTATTTTAAATCTCACCAGTCTTGTTACTTTGAGTCTGAATGATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATTTCTCACATGAAATCTCTTGAATACCTTTCTGTTTCTGGATGCTCAAAGCTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGAATAATCTATTTAGGCTTTATTTGGATGGCACTGCAATTAAGGAGGTGCCTGCTTCTATTTTAAATCTCACCAGTCTTGTTACTTTGAAGCTGAAGGATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATTTCTCACATGAAATCTCTTGAATACCTTTCTGTTTCTGGATGCTCAAAGCTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGAATAATCTATTTAAACTTTATTTGGATGGCATTGCAATTAAGGAGCTGCCTGCTTCTATTTTAAATCTCACCAGTCTCGTTACTTTGAAGCTGAAGGATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATTTCTCACATGAAATCTCTTGAATACCTTTCTGTTTCTGGATGCTCAAAGCTTGAGAAGTTTCCAGAAATTTCAGAGGTCATGAAGAAGCTATTTAAGCTTTATTTGGGTGGCACCGCAATTAAGGAGCTGCCTGCTTCTATTTTAAATCTCACCAGTCTCGTTACTTTGAAGCTGAATGATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATTTCTCACATGAAATCTCTTAAATACCTTTATGTTTCTGGATGCTCAAAGCTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGAATAATCTATTTAAGCTTTATTTAGGTGGCACTGCAATTAAGGAATTGCCTCCTTCAGTTTTAAATCTCATAAGTCTTGTTACTTTGAAGTTGAATGATTGTAGAGAACTTGAGAGTCTTCCAAGCACCATTTGTCATCTGAAATCTCTTCAATACCTTGATGTTTCTAGATGCTCAAAGCTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGAATAATCTATTTAAACTTTATTTGGATGGCACTGCAATTAAGGAGTTGCCTGCTTCTATTTTAAATCTCACCAGTCTCGTTACTTTGAAGCTGAAGGATTGCAGAGAACTTGAGTCTTCCAAGCAGCATTTCTCACATGAAATCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1069

Amino Acids

119.18

Weight (kDa)

6.07

Isoelectric Point (pI)

31.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_13 PF23286 2 - 69 8e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 3 - 87 2.7e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 28 - 173 8.7e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_13 PF23286 72 - 157 8.7e-14 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 102 - 174 1.5e-08 Leucine-rich repeat region
LRR_13 PF23286 144 - 227 5.9e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 173 - 245 1.6e-09 Leucine-rich repeat region
LRR_13 PF23286 214 - 283 5.9e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_Zer-1 PF25013 235 - 312 2.9e-06 Zer-1-like, Leucine-rich repeats
LRR_14 PF23598 244 - 312 3.4e-06 Leucine-rich repeat region
LRR_13 PF23286 285 - 354 3.1e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 315 - 386 1.6e-07 Leucine-rich repeat region
LRR_13 PF23286 356 - 425 9.6e-13 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 386 - 440 1.5e-06 Leucine-rich repeat region
LRR_13 PF23286 432 - 511 5.1e-13 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 457 - 525 8.6e-08 Leucine-rich repeat region
LRR_13 PF23286 499 - 583 2.9e-13 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 528 - 588 8.8e-07 Leucine-rich repeat region
LRR_13 PF23286 570 - 654 7.4e-13 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 599 - 668 6.9e-07 Leucine-rich repeat region
LRR_13 PF23286 641 - 724 6.5e-14 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 670 - 729 6.2e-08 Leucine-rich repeat region
LRR_13 PF23286 711 - 779 1e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 741 - 807 1.4e-07 Leucine-rich repeat region
LRR_13 PF23286 782 - 851 7.9e-13 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 812 - 880 1.6e-07 Leucine-rich repeat region
LRR_13 PF23286 853 - 938 1.5e-15 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 883 - 951 1.4e-08 Leucine-rich repeat region
LRR_13 PF23286 925 - 994 3.3e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 951 - 1020 6.9e-08 Leucine-rich repeat region
LRR_13 PF23286 995 - 1057 6.2e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2050
AccB1I GGYRCC 2 cut(s) 2251, 2660
AciI CCGC 1 cut(s) 2664
AcuI CTGAAG 4 cut(s) 2318, 2531, 2883, 3170
AcvI CACGTG 1 cut(s) 1918
AfiI CCNNNNNNNGG 1 cut(s) 1588
AflII CTTAAG 1 cut(s) 1250
Alw26I GTCTC 3 cut(s) 2498, 2711, 3137
Asp700I GAANNNNTTC 1 cut(s) 192
AspLEI GCGC 1 cut(s) 2008
BanI GGYRCC 2 cut(s) 2251, 2660
BbrPI CACGTG 1 cut(s) 1918
BccI CCATC 8 cut(s) 95, 1160, 1586, 1799, 2012, 2225, 2438, 3077
BcoDI GTCTC 3 cut(s) 2498, 2711, 3137
BfaI CTAG 2 cut(s) 30, 3012
BfrI CTTAAG 1 cut(s) 1250
BfuAI ACCTGC 1 cut(s) 2050
BmiI GGNNCC 2 cut(s) 2253, 2662
BsaAI YACGTR 1 cut(s) 1918
Bsc4I CCNNNNNNNGG 1 cut(s) 1588
Bse3DI GCAATG 2 cut(s) 1383, 2448
BseLI CCNNNNNNNGG 1 cut(s) 1588
BseMI GCAATG 2 cut(s) 1383, 2448
BseMII CTCAG 3 cut(s) 78, 504, 717
BshNI GGYRCC 2 cut(s) 2251, 2660
BslI CCNNNNNNNGG 1 cut(s) 1588
BsmAI GTCTC 3 cut(s) 2498, 2711, 3137
BspACI CCGC 1 cut(s) 2664
BspCNI CTCAG 3 cut(s) 79, 505, 718
BspHI TCATGA 1 cut(s) 2628
BspLI GGNNCC 2 cut(s) 2253, 2662
BspMI ACCTGC 1 cut(s) 2050
BspT107I GGYRCC 2 cut(s) 2251, 2660
BspTI CTTAAG 1 cut(s) 1250
BsrDI GCAATG 2 cut(s) 1383, 2448
Bst6I CTCTTC 2 cut(s) 1080, 2997
BstAFI CTTAAG 1 cut(s) 1250
BstBAI YACGTR 1 cut(s) 1918
BstDEI CTNAG 3 cut(s) 87, 513, 726
BstHHI GCGC 1 cut(s) 2008
BstMAI GTCTC 3 cut(s) 2498, 2711, 3137
BstMWI GCNNNNNNNGC 1 cut(s) 2644
BstNSI RCATGY 1 cut(s) 1832
BveI ACCTGC 1 cut(s) 2050
CciI TCATGA 1 cut(s) 2628
CfoI GCGC 1 cut(s) 2008
DdeI CTNAG 3 cut(s) 87, 513, 726
Eam1104I CTCTTC 2 cut(s) 1080, 2997
EarI CTCTTC 2 cut(s) 1080, 2997
Eco32I GATATC 1 cut(s) 297
Eco57I CTGAAG 4 cut(s) 2318, 2531, 2883, 3170
Eco72I CACGTG 1 cut(s) 1918
EcoRV GATATC 1 cut(s) 297
FauNDI CATATG 1 cut(s) 1065
FspBI CTAG 2 cut(s) 30, 3012
GlaI GCGC 1 cut(s) 2007
HhaI GCGC 1 cut(s) 2008
Hin6I GCGC 1 cut(s) 2006
HinP1I GCGC 1 cut(s) 2006
HincII GTYRAC 1 cut(s) 1381
HindII GTYRAC 1 cut(s) 1381
Hpy166II GTNNAC 1 cut(s) 1381
Hpy8I GTNNAC 1 cut(s) 1381
HpyAV CCTTC 5 cut(s) 1307, 2293, 2506, 2907, 3145
HpyCH4IV ACGT 1 cut(s) 1917
HpyF10VI GCNNNNNNNGC 1 cut(s) 2644
HpyF3I CTNAG 3 cut(s) 87, 513, 726
HpySE526I ACGT 1 cut(s) 1917
HspAI GCGC 1 cut(s) 2006
LmnI GCTCC 9 cut(s) 118, 544, 757, 1183, 1396, 1822, 2035, 2461, 2674
MaeI CTAG 2 cut(s) 30, 3012
MaeII ACGT 1 cut(s) 1917
MroXI GAANNNNTTC 1 cut(s) 192
MspCI CTTAAG 1 cut(s) 1250
MwoI GCNNNNNNNGC 1 cut(s) 2644
NdeI CATATG 1 cut(s) 1065
NlaIV GGNNCC 2 cut(s) 2253, 2662
NspI RCATGY 1 cut(s) 1832
PagI TCATGA 1 cut(s) 2628
PdmI GAANNNNTTC 1 cut(s) 192
PfeI GAWTC 3 cut(s) 79, 505, 718
PmaCI CACGTG 1 cut(s) 1918
PmlI CACGTG 1 cut(s) 1918
Ppu21I YACGTR 1 cut(s) 1918
PspCI CACGTG 1 cut(s) 1918
PspN4I GGNNCC 2 cut(s) 2253, 2662
SsiI CCGC 1 cut(s) 2664
SspMI CTAG 2 cut(s) 30, 3012
TaiI ACGT 1 cut(s) 1920
TfiI GAWTC 3 cut(s) 79, 505, 718
TspGWI ACGGA 1 cut(s) 969
Vha464I CTTAAG 1 cut(s) 1250
XbaI TCTAGA 2 cut(s) 29, 3011
XceI RCATGY 1 cut(s) 1832
XmnI GAANNNNTTC 1 cut(s) 192
XspI CTAG 2 cut(s) 30, 3012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.