pycom17g26060

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
N/A
Physical Location & Seq
Reverse (-)
24370333 .. 24373301
2969 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1209 bp
ATGTTGATGAATTGTCGATATTTTTTTGTTTTAAATTCAAGAGTCCCTTTCTTGTCCTCTCTCCTTCAAAGCCCCCGACTTCCTCCCTCCGAACTCGACATTACTTCTTCACTCTCTCTCTCTGTCACTCAAGAAGGAAGCAAAAATCTAGAGAGAGAGAAAGAGAAACTGAAGCAAAGCATGGACGAGAATTCATCGGCACTAATCGAAGCAATTCTCAGAGAGCAAGAGGAGGAGGAGGCACAGAGATACGGCAAGAAGTTGACCCAAAACGGCGACGCTTACGCCTGGCAAACGGTATCGTATCCGAAGCGAAACCGGAAATCCTCCTCCAAGGCGATTCCTGCGGATTCCAACGGTGGTCTCCATAACGGTGTCGTATCCTCCGCCTCCGACGTGTTTCGGCCGATCGAGCTGCATTCCGAGGAGCGCCGTCGGAGAGTTCTGGAGGCTCAGTCCGCAGCCGTTTTCGGAGACGCCGCACCAGGCGGATCGAAGCGGAATTCAGATGACGACGATGAGGATAATAGCGATGCTGAGGTGGCCGGGGCGGCCGTCGAGAACGTCGAGGTGAAGAAGGTGAAGCAGAAGAAGCCGAAGAAGCCGAAGGTGACGGTGGCGGAGGCGGCGTCGAAAATGGACGCCGGTGAACTCGGTGCTTTTCTGGCAGATATAACTGCATCGTATGAAACGCAGCAGGACGTACAGCTTATGCGTTTGGCGGATTATTTCGGCCGAGCATTTGCGGCGGTCAGTCCGGCACAATTTCCGTGGTTGAAGACGTTCAAGGAATCTTCTGTGGCAAAGCTGGTTGATATCCCTCTTTCGCATATATCTGAAGATGTTTACAAGATATCGGTTGAATGGATCAGCCAGCGCTCTACTGAAGGGCTTGGATCATTCGTGCTATGGTCGTTGGACAGCATTCTTGCTGACCTTGCAAGTCATCAAGGAGTAGCCAAGGGATCCAAAAAGGCGGTCCAGCAAGCACCTTCAAAGTCTCAGGTTGCCATATTCGTGGTTTTAGCAATGGTATTGCGACGAAGACCTGAAGTACTGATTAGCTTACATCCTGTGATGAGGGGAAGTCTGAAATATGAAGGCCAAGATAAGCTTCCAATCACAGTTTGGCTGGTTGCAGGCTTCTCAAGGAGATCTGGTTGTGGGGTTGTACTCGTGGGTGCATTTTCTCTTGCCTATATTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

403

Amino Acids

44.14

Weight (kDa)

7.7

Isoelectric Point (pI)

53.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 5 cut(s) 499, 875, 904, 960, 973
AcoI YGGCCR 4 cut(s) 404, 543, 552, 733
AcsI RAATTY 3 cut(s) 34, 190, 502
AcuI CTGAAG 4 cut(s) 191, 858, 906, 1071
AcyI GRCGYC 3 cut(s) 477, 629, 642
AfaI GTAC 3 cut(s) 705, 1056, 1173
AfeI AGCGCT 1 cut(s) 878
AflIII ACRYGT 1 cut(s) 396
AgsI TTSAA 6 cut(s) 39, 68, 778, 787, 863, 996
AjiI CACGTC 1 cut(s) 397
AjnI CCWGG 2 cut(s) 287, 484
AjuI GAANNNNNNNTTGG 2 cut(s) 1098, 1130
AluBI AGCT 5 cut(s) 415, 709, 808, 1065, 1114
AluI AGCT 5 cut(s) 415, 709, 808, 1065, 1114
Alw26I GTCTC 3 cut(s) 368, 468, 1005
AlwI GGATC 5 cut(s) 499, 875, 904, 960, 973
Aor51HI AGCGCT 1 cut(s) 878
AoxI GGCC 5 cut(s) 404, 543, 552, 733, 1102
ApeKI GCWGC 3 cut(s) 415, 461, 694
ApoI RAATTY 3 cut(s) 34, 190, 502
ArsI GACNNNNNNTTYG 2 cut(s) 963, 995
Asp700I GAANNNNTTC 2 cut(s) 213, 782
AspLEI GCGC 2 cut(s) 432, 879
AspS9I GGNCC 1 cut(s) 979
AsuC2I CCSGG 1 cut(s) 547
AsuHPI GGTGA 4 cut(s) 583, 592, 622, 659
AvaII GGWCC 1 cut(s) 979
BamHI GGATCC 1 cut(s) 965
BauI CACGAG 1 cut(s) 1175
BbsI GAAGAC 2 cut(s) 785, 1051
BbvCI CCTCAGC 1 cut(s) 537
BbvI GCAGC 3 cut(s) 402, 473, 706
BceAI ACGGC 5 cut(s) 268, 289, 417, 449, 539
BcgI CGANNNNNNTGC 2 cut(s) 397, 431
BciT130I CCWGG 2 cut(s) 289, 486
BciVI GTATCC 2 cut(s) 315, 391
BcnI CCSGG 1 cut(s) 547
BcoDI GTCTC 3 cut(s) 368, 468, 1005
BfaI CTAG 1 cut(s) 149
BfoI RGCGCY 2 cut(s) 433, 880
BfuI GTATCC 2 cut(s) 315, 391
BglI GCCNNNNNGGC 1 cut(s) 551
BglII AGATCT 1 cut(s) 1154
BisI GCNGC 7 cut(s) 416, 462, 480, 552, 627, 695, 747
BlsI GCNGC 7 cut(s) 417, 463, 481, 553, 628, 696, 748
BmcAI AGTACT 1 cut(s) 1056
Bme1390I CCNGG 3 cut(s) 289, 486, 547
Bme18I GGWCC 1 cut(s) 979
BmgBI CACGTC 1 cut(s) 397
BmgT120I GGNCC 1 cut(s) 979
BmiI GGNNCC 1 cut(s) 967
BmrFI CCNGG 3 cut(s) 289, 486, 547
BmsI GCATC 2 cut(s) 523, 689
BpiI GAAGAC 2 cut(s) 785, 1051
BpmI CTGGAG 1 cut(s) 467
Bpu10I CCTNAGC 1 cut(s) 537
BpuEI CTTGAG 2 cut(s) 114, 1132
BpuMI CCSGG 1 cut(s) 547
BsaHI GRCGYC 3 cut(s) 477, 629, 642
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 5 cut(s) 333, 423, 546, 770, 960
BsaWI WCCGGW 1 cut(s) 318
BsaXI ACNNNNNCTCC 6 cut(s) 440, 470, 614, 644, 1144, 1174
Bse118I RCCGGY 1 cut(s) 644
Bse3DI GCAATG 1 cut(s) 1035
BseBI CCWGG 2 cut(s) 289, 486
BseDI CCNNGG 5 cut(s) 333, 423, 546, 770, 960
BseGI GGATG 1 cut(s) 1069
BseMI GCAATG 1 cut(s) 1035
BseMII CTCAG 4 cut(s) 232, 467, 528, 1016
BseRI GAGGAG 5 cut(s) 245, 248, 251, 319, 440
BseX3I CGGCCG 3 cut(s) 404, 552, 733
BseXI GCAGC 3 cut(s) 402, 473, 706
Bsh1285I CGRYCG 4 cut(s) 407, 411, 555, 736
BshFI GGCC 5 cut(s) 406, 545, 554, 735, 1104
BsiEI CGRYCG 4 cut(s) 407, 411, 555, 736
BsiSI CCGG 4 cut(s) 319, 546, 645, 758
BslFI GGGAC 1 cut(s) 29
BsmAI GTCTC 3 cut(s) 368, 468, 1005
BsmBI CGTCTC 1 cut(s) 468
BsmFI GGGAC 1 cut(s) 29
BsmI GAATGC 2 cut(s) 418, 924
BsnI GGCC 5 cut(s) 406, 545, 554, 735, 1104
Bso31I GGTCTC 1 cut(s) 368
Bsp143I GATC 6 cut(s) 408, 491, 867, 896, 965, 1154
BspANI GGCC 5 cut(s) 406, 545, 554, 735, 1104
BspCNI CTCAG 4 cut(s) 231, 466, 529, 1015
BspLI GGNNCC 1 cut(s) 967
BspPI GGATC 5 cut(s) 499, 875, 904, 960, 973
BspTNI GGTCTC 1 cut(s) 368
BsrDI GCAATG 1 cut(s) 1035
BsrFI RCCGGY 1 cut(s) 644
BssAI RCCGGY 1 cut(s) 644
BssECI CCNNGG 5 cut(s) 333, 423, 546, 770, 960
BssMI GATC 6 cut(s) 408, 491, 867, 896, 965, 1154
BssNI GRCGYC 3 cut(s) 477, 629, 642
BssSI CACGAG 1 cut(s) 1175
BssT1I CCWWGG 2 cut(s) 333, 960
Bst2BI CACGAG 1 cut(s) 1175
Bst2UI CCWGG 2 cut(s) 289, 486
Bst4CI ACNGT 5 cut(s) 298, 359, 374, 616, 1126
BstACI GRCGYC 3 cut(s) 477, 629, 642
BstC8I GCNNGC 3 cut(s) 875, 987, 1141
BstDEI CTNAG 4 cut(s) 218, 453, 537, 1002
BstDSI CCRYGG 1 cut(s) 770
BstF5I GGATG 1 cut(s) 1069
BstH2I RGCGCY 2 cut(s) 433, 880
BstHHI GCGC 2 cut(s) 432, 879
BstKTI GATC 6 cut(s) 411, 494, 870, 899, 968, 1157
BstMAI GTCTC 3 cut(s) 368, 468, 1005
BstMBI GATC 6 cut(s) 408, 491, 867, 896, 965, 1154
BstMCI CGRYCG 4 cut(s) 407, 411, 555, 736
BstNI CCWGG 2 cut(s) 289, 486
BstSCI CCNGG 3 cut(s) 287, 484, 545
BstV1I GCAGC 3 cut(s) 402, 473, 706
BstV2I GAAGAC 2 cut(s) 785, 1051
BstX2I RGATCY 2 cut(s) 965, 1154
BstXI CCANNNNNNTGG 1 cut(s) 1018
BstYI RGATCY 2 cut(s) 965, 1154
BstZI CGGCCG 3 cut(s) 404, 552, 733
BsuI GTATCC 2 cut(s) 315, 391
BsuRI GGCC 5 cut(s) 406, 545, 554, 735, 1104
BtgI CCRYGG 1 cut(s) 770
BtgZI GCGATG 1 cut(s) 546
BtrI CACGTC 1 cut(s) 397
BtsCI GGATG 1 cut(s) 1069
Cac8I GCNNGC 3 cut(s) 875, 987, 1141
CfoI GCGC 2 cut(s) 432, 879
Cfr10I RCCGGY 1 cut(s) 644
Cfr13I GGNCC 1 cut(s) 979
CseI GACGC 4 cut(s) 287, 485, 618, 650
Csp6I GTAC 3 cut(s) 704, 1055, 1172
CspCI CAANNNNNGTGG 2 cut(s) 752, 787
CviAII CATG 1 cut(s) 181
CviQI GTAC 3 cut(s) 704, 1055, 1172
DdeI CTNAG 4 cut(s) 218, 453, 537, 1002
DpnI GATC 6 cut(s) 410, 493, 869, 898, 967, 1156
DpnII GATC 6 cut(s) 408, 491, 867, 896, 965, 1154
DraI TTTAAA 1 cut(s) 33
EaeI YGGCCR 4 cut(s) 404, 543, 552, 733
EagI CGGCCG 3 cut(s) 404, 552, 733
EciI GGCGGA 4 cut(s) 376, 504, 635, 737
EclXI CGGCCG 3 cut(s) 404, 552, 733
Eco130I CCWWGG 2 cut(s) 333, 960
Eco31I GGTCTC 1 cut(s) 368
Eco32I GATATC 2 cut(s) 817, 855
Eco47I GGWCC 1 cut(s) 979
Eco47III AGCGCT 1 cut(s) 878
Eco52I CGGCCG 3 cut(s) 404, 552, 733
Eco57I CTGAAG 4 cut(s) 191, 858, 906, 1071
EcoRI GAATTC 2 cut(s) 190, 502
EcoRII CCWGG 2 cut(s) 287, 484
EcoRV GATATC 2 cut(s) 817, 855
EcoT14I CCWWGG 2 cut(s) 333, 960
ErhI CCWWGG 2 cut(s) 333, 960
Esp3I CGTCTC 1 cut(s) 468
FaeI CATG 1 cut(s) 184
FalI AAGNNNNNCTT 4 cut(s) 31, 63, 1098, 1130
FaqI GGGAC 1 cut(s) 29
FatI CATG 1 cut(s) 180
Fnu4HI GCNGC 7 cut(s) 416, 462, 480, 552, 627, 695, 747
FokI GGATG 1 cut(s) 1056
Fsp4HI GCNGC 7 cut(s) 416, 462, 480, 552, 627, 695, 747
FspBI CTAG 1 cut(s) 149
GlaI GCGC 2 cut(s) 431, 878
GluI GCNGC 7 cut(s) 416, 462, 480, 552, 627, 695, 747
GsuI CTGGAG 1 cut(s) 467
HaeII RGCGCY 2 cut(s) 433, 880
HaeIII GGCC 5 cut(s) 406, 545, 554, 735, 1104
HapII CCGG 4 cut(s) 319, 546, 645, 758
HgaI GACGC 4 cut(s) 287, 485, 618, 650
HhaI GCGC 2 cut(s) 432, 879
Hin1I GRCGYC 3 cut(s) 477, 629, 642
Hin1II CATG 1 cut(s) 184
Hin6I GCGC 2 cut(s) 430, 877
HinP1I GCGC 2 cut(s) 430, 877
HincII GTYRAC 1 cut(s) 264
HindII GTYRAC 1 cut(s) 264
HindIII AAGCTT 1 cut(s) 1112
HinfI GANTC 4 cut(s) 42, 340, 350, 791
HpaII CCGG 4 cut(s) 319, 546, 645, 758
HphI GGTGA 4 cut(s) 583, 592, 622, 659
Hpy166II GTNNAC 3 cut(s) 264, 650, 847
Hpy188III TCNNGA 5 cut(s) 39, 131, 149, 446, 559
Hpy8I GTNNAC 3 cut(s) 264, 650, 847
Hpy99I CGWCG 8 cut(s) 281, 398, 438, 518, 560, 569, 634, 1044
HpyAV CCTTC 7 cut(s) 74, 128, 571, 601, 881, 1002, 1094
HpyCH4III ACNGT 5 cut(s) 298, 359, 374, 616, 1126
HpyCH4IV ACGT 4 cut(s) 396, 564, 702, 782
HpyCH4V TGCA 5 cut(s) 418, 680, 941, 1139, 1184
HpyF3I CTNAG 4 cut(s) 218, 453, 537, 1002
HpySE526I ACGT 4 cut(s) 396, 564, 702, 782
Hsp92I GRCGYC 3 cut(s) 477, 629, 642
Hsp92II CATG 1 cut(s) 184
HspAI GCGC 2 cut(s) 430, 877
Kzo9I GATC 6 cut(s) 408, 491, 867, 896, 965, 1154
LmnI GCTCC 1 cut(s) 427
Lsp1109I GCAGC 3 cut(s) 402, 473, 706
LweI GCATC 2 cut(s) 523, 689
MaeI CTAG 1 cut(s) 149
MaeII ACGT 4 cut(s) 396, 564, 702, 782
MaeIII GTNAC 2 cut(s) 124, 610
MalI GATC 6 cut(s) 410, 493, 869, 898, 967, 1156
MboI GATC 6 cut(s) 408, 491, 867, 896, 965, 1154
MboII GAAGA 8 cut(s) 99, 586, 601, 610, 786, 790, 851, 1056
MflI RGATCY 2 cut(s) 965, 1154
MluCI AATT 6 cut(s) 10, 34, 190, 213, 502, 764
MlyI GAGTC 1 cut(s) 51
MmeI TCCRAC 4 cut(s) 378, 416, 417, 897
MroXI GAANNNNTTC 2 cut(s) 213, 782
MseI TTAA 1 cut(s) 32
MslI CAYNNNNRTG 2 cut(s) 372, 1016
MspI CCGG 4 cut(s) 319, 546, 645, 758
MspR9I CCNGG 3 cut(s) 289, 486, 547
Mva1269I GAATGC 2 cut(s) 418, 924
MvaI CCWGG 2 cut(s) 289, 486
NciI CCSGG 1 cut(s) 547
NdeII GATC 6 cut(s) 408, 491, 867, 896, 965, 1154
NlaIII CATG 1 cut(s) 184
NlaIV GGNNCC 1 cut(s) 967
NmeAIII GCCGAG 1 cut(s) 761
NmuCI GTSAC 2 cut(s) 124, 610
PcsI WCGNNNNNNNCGW 1 cut(s) 564
PctI GAATGC 2 cut(s) 418, 924
PdmI GAANNNNTTC 2 cut(s) 213, 782
PfeI GAWTC 3 cut(s) 340, 350, 791
PkrI GCNGC 7 cut(s) 417, 463, 481, 553, 628, 696, 748
Ple19I CGATCG 1 cut(s) 411
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
Psp6I CCWGG 2 cut(s) 287, 484
PspGI CCWGG 2 cut(s) 287, 484
PspN4I GGNNCC 1 cut(s) 967
PspPI GGNCC 1 cut(s) 979
PsuI RGATCY 2 cut(s) 965, 1154
PvuI CGATCG 1 cut(s) 411
RsaI GTAC 3 cut(s) 705, 1056, 1173
RsaNI GTAC 3 cut(s) 704, 1055, 1172
RseI CAYNNNNRTG 2 cut(s) 372, 1016
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 7 cut(s) 416, 462, 480, 552, 627, 695, 747
Sau3AI GATC 6 cut(s) 408, 491, 867, 896, 965, 1154
Sau96I GGNCC 1 cut(s) 979
ScaI AGTACT 1 cut(s) 1056
SchI GAGTC 1 cut(s) 51
ScrFI CCNGG 3 cut(s) 289, 486, 547
SfaNI GCATC 2 cut(s) 523, 689
SfiI GGCCNNNNNGGCC 1 cut(s) 551
SgrAI CRCCGGYG 1 cut(s) 644
SinI GGWCC 1 cut(s) 979
SmiMI CAYNNNNRTG 2 cut(s) 372, 1016
SmlI CTYRAG 2 cut(s) 129, 1147
SmoI CTYRAG 2 cut(s) 129, 1147
Sse9I AATT 6 cut(s) 10, 34, 190, 213, 502, 764
SspMI CTAG 1 cut(s) 149
StyD4I CCNGG 3 cut(s) 287, 484, 545
StyI CCWWGG 2 cut(s) 333, 960
TaaI ACNGT 5 cut(s) 298, 359, 374, 616, 1126
TaiI ACGT 4 cut(s) 399, 567, 705, 785
TaqI TCGA 8 cut(s) 16, 96, 207, 411, 494, 558, 567, 632
TasI AATT 6 cut(s) 10, 34, 190, 213, 502, 764
TatI WGTACW 2 cut(s) 1054, 1171
TauI GCSGC 4 cut(s) 482, 554, 629, 749
TfiI GAWTC 3 cut(s) 340, 350, 791
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TseFI GTSAC 2 cut(s) 124, 610
TseI GCWGC 3 cut(s) 415, 461, 694
Tsp45I GTSAC 2 cut(s) 124, 610
TspDTI ATGAA 4 cut(s) 23, 183, 702, 1113
TspGWI ACGGA 1 cut(s) 759
VpaK11BI GGWCC 1 cut(s) 979
XapI RAATTY 3 cut(s) 34, 190, 502
XbaI TCTAGA 1 cut(s) 148
XcmI CCANNNNNNNNNTGG 1 cut(s) 1125
XmnI GAANNNNTTC 2 cut(s) 213, 782
XspI CTAG 1 cut(s) 149
ZrmI AGTACT 1 cut(s) 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.