pycom17g26210

Importin-5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0028768
Physical Location & Seq
Reverse (-)
24562401 .. 24569123
6723 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2982 bp
ATGAACCAGCTGGACCAAGACGCCGGAGAGCTTCTTGAATCTCCATTCCCCCTACCCATGGAGTCTCTACTCGCAACCATCATCCTCCTCCCCATAAACAGCCCCCTTGCCCAACAAAAACGAGAGCTCCTTCAACACTGCTCTTTCCAATTCACCGATGCCCTCTCCCTTAAGCTCTTCCAGGTCTCCAGCTCCTCCCACTACCCCCGAATCCGCCGCAAATCACTGAAACTCCTCCACAATCTACTCACCGACCCCGCCGTTCCCCAAATCTCCCTCATGATCCTCAACGGCCTCAAATCCCCGCTCTTGTCCAGTTTTCGACCCAAGACCGGACAACCCATGTCTCAAATTCTGCCCGAAATCGTCGCCGTTGTCGCTTCTCGAATCTTCAGCGGCTCCGACCAGCTGTGGGAAGAGCTCCTCGACTATGTTTACGTCTCCATTACCAGCTCCAACGTCACTCAAGAAGTCCAAGAAATGGGATTCATGGTACTCTGCAATCTTCCAGATAATCTTTTGAATCGGTTGATACCGGGATTCGATTTTCTTTGGGCGAAAATCATGCACCGTTTGCGAGATTCCTCTGATTTGGATTCCTGGGCGGCGGCGATCCAGGCGGCGTCCAGATTTCTCAAGATTTTGCCGTATTTCTTGGGGGCCGACGGCCTTGGAATGGCCATGTCTACAGTGGTCAATTCGACACACAAGTTGGCCACGAAAGTTGACCCGAGAAGCGCAACGGTGGCTCAGAGGTTCCTCGAGTTTATAACCCAAATGGTGACTAAAACGCAACCGAGACTCTTGCTCAACCGATTAAACTACGTGTTGGAATCAATGCTGATGATCGCGGAGTCTGCGGTGGCCACGGTGATCAGAGAGGGCGCAATCAGAGTACTTAAAAAGATCGACGAATCGAACAGAAGGATTATGAGTCGTCAACTCGAAAGATTGGATCGTGTGAATTTGGATGGGTTTTTCTTCAGCCTGATGAGGGTGCTTGTGGGGGCTGCAAATGTCGAGAAAAACAAGAGCTCTGTTCTTGCGAAGTATTTTATAGACCGGCTTTCGACTATGCACGGAGGGAAGATTTTTGCGCGTCTTCTTCGGAATCAGATCCAAGTTTTTGCGGCGGCTCAGGAATGGCAGAAACGCCATGCAGGGCTTGTTACTATTTGTGCCACGGCTGATGCGTGTGAAGAGGTTATGGGAAACCATGTTGAGGAATTGGCAACAATAGTTCTAGATAGCCTAGAAGATCCTCACTATCGAGTTCGTTGTGCTGCCACCGATGCCATTAAGGCACTCAGTGTGAACTTAGCACCTACATTACATAAGCATTATCATCGCGCGATTCTACGTCTGCTAACTGAAATGCTTAAAGAAGAGAACGACACCATATGGGGGAAAGCCATTCAAGCAATTCGTTTCTTCAGCAATGGCTGCACACCCGATAATTTAGAACCTTACTTGCCTCAGTTAGTGGGGGCACTGCTTGCACATCTTAAGAACGGGAATATAACTCTACAATGGGGGGTTTTGGCAGCTCTGGGAGCAGTTGCAACTTCGTCGGAGAACCGCTTTGATATTTACTATCGCCACACTATGGATGCCATTAAACTTATCTTGTTCGGTGTAGCTAATGCATCTACAAAATTGCTCCATGCCCATTGTCTGGAGTGCATTAGTTATATTGCAAGTGTTGTTGGAAAGGATAACTTTGCAAACGATGCTAACGAGGTTATGGGAGCTCTTATCTCGCTGCTAGAATTTCTGCTGGAGACAGACCACATGGTAAAAGTTTCCATCTTCCAGGTGTTGGCAAAACTCTGTAGGCTCCTAGGGGATGACTTTTTAATCTATGTCGACGATCTGATGCCTAGCTTGATTCAGTCTGCTCAGAATAAAATCAATAAAGAAGTCAACCGAAGGGAGAAAGCTTTGGCCTGTAAATTGCTATTCTGCTTCACCAGCCAGTTTAAGGGAAAGTTCTTGCCTTGGATCAAACAGGTTACTCCGGTTTTGCGTCCACTTCTTCACTCGGATCTACATTTTGAAACTCGAATGCAAGCTATTTCAGCCATGCCGGTCCTGTTGCGTTTAACTATATCTCAAGAAGTTATTTTGGAAGATATCCAGGAGGAGCTTATCTACTTTATAACAGCATTAGTAAGAAACTTGAAGGAAAATGATGCAGAAATTTCTGCGAGAGTGTTAAATTCTCTAAAAGAGTGCATAAAGGTCTCTGGCCGCATTTTGACTAATATACATGTCAAGTTTGTTGTGGATGAGATACAGAAAATCCTGAACGAAAACTCTTCAGAAAACGAAATACAAGAAGCAGAGGGTTTTGAAATTGGACCGCAGGAAGAAACCCGTAATCGTGGTGATGATCATACAGTTAATGCAAAAATTGTTGTGTTTCTGTTCAACTATTTTGTGCAACAAAGCCAAGAACCTGCTTCTAAATATTGTTCTACATTCCTTCCATTCCTCCTGAGAGCTTGCAAGGATCATGATCCATGTGTTAGACAGCAAGCTGTACGCGGAATTGGTACTTGTGCAGAGTTTGGCGTTGATGAATTCAAACCTTTTGCTGAAGAGGCTGCCAAAAGTCTGGATTCTGTGATTAGACATCCAGGCACATCAAGCTCAAACAATCCAACGGCACATGTGATTGTCGTGTCATCTCTTGGGAGAATATGTGGGTCTTATTTCAAGAGTATTAATGCTCCTCAGGTTGTTGGCAAATGGTTGGACTTCTTACCACTGAAAAATGACACCACTGAAGCCAAACTCGTACATGCACGACTCTCTGCAATGATTGAAGAGTTTCACGACATTCTTTTGGGCGTTAACGGTCAATATAAGTCTAGGATTTTGGAGATCTTTGCTCAGGTTGTAGCTGACGGTGATAATCTTGCAACAAGGGATACTGTTAACAGGATGGTTGTTCAATTAGTCCAGTGTAGCAGGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HEAT HEAT_2

Protein Analysis

994

Amino Acids

111.41

Weight (kDa)

6.83

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF577 PF04510 100 - 240 1.8e-06 Repeat of unknown function (DUF577)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028768)

Species Orthologous Gene IDs
malus_domestica MD17G1262200.v1.1
pyrus_communis pycom17g26210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 770, 2159
Acc36I ACCTGC 1 cut(s) 2467
AccB7I CCANNNNNTGG 4 cut(s) 481, 1606, 1675, 1819
AccBSI CCGCTC 1 cut(s) 307
AccI GTMKAC 2 cut(s) 686, 1866
AccII CGCG 5 cut(s) 851, 1099, 1350, 1352, 2547
AclWI GGATC 9 cut(s) 277, 607, 963, 1111, 1253, 2009, 2052, 2513, 2520
AcoI YGGCCR 4 cut(s) 678, 714, 864, 2248
AcsI RAATTY 6 cut(s) 351, 964, 1769, 2199, 2218, 2582
AcuI CTGAAG 6 cut(s) 376, 967, 1417, 2304, 2619, 2808
AcyI GRCGYC 2 cut(s) 21, 623
AdeI CACNNNGTG 1 cut(s) 1310
AfaI GTAC 5 cut(s) 495, 897, 2544, 2557, 2802
AflII CTTAAG 2 cut(s) 170, 1505
AflIII ACRYGT 3 cut(s) 825, 2269, 2671
AjnI CCWGG 6 cut(s) 180, 599, 615, 1812, 2136, 2638
Alw21I GWGCWC 4 cut(s) 129, 423, 1037, 1753
Alw26I GTCTC 7 cut(s) 69, 190, 351, 445, 793, 1775, 2248
AlwI GGATC 9 cut(s) 277, 607, 963, 1111, 1253, 2009, 2052, 2513, 2520
Ama87I CYCGRG 2 cut(s) 730, 761
AoxI GGCC 9 cut(s) 292, 660, 667, 678, 714, 864, 1944, 2248, 2975
ApeKI GCWGC 6 cut(s) 1010, 1283, 1443, 1544, 1762, 2606
ApoI RAATTY 6 cut(s) 351, 964, 1769, 2199, 2218, 2582
AseI ATTAAT 1 cut(s) 2727
Asp700I GAANNNNTTC 1 cut(s) 2832
AspA2I CCTAGG 1 cut(s) 1840
AspLEI GCGC 4 cut(s) 740, 887, 1099, 1352
AspS9I GGNCC 4 cut(s) 13, 660, 2089, 2360
AsuC2I CCSGG 1 cut(s) 537
AsuHPI GGTGA 7 cut(s) 145, 241, 793, 883, 1960, 2399, 2924
AvaI CYCGRG 2 cut(s) 730, 761
AvaII GGWCC 3 cut(s) 13, 2089, 2360
AvrII CCTAGG 1 cut(s) 1840
AxyI CCTNAGG 1 cut(s) 2736
BaeGI GKGCMC 1 cut(s) 1492
BalI TGGCCA 3 cut(s) 680, 716, 866
BanII GRGCYC 4 cut(s) 129, 423, 1037, 1753
BbsI GAAGAC 1 cut(s) 1094
Bbv12I GWGCWC 4 cut(s) 129, 423, 1037, 1753
BbvI GCAGC 6 cut(s) 997, 1270, 1430, 1556, 1749, 2593
BccI CCATC 4 cut(s) 86, 965, 1814, 2941
BceAI ACGGC 7 cut(s) 245, 307, 356, 631, 682, 1200, 2682
BcgI CGANNNNNNTGC 8 cut(s) 547, 581, 787, 821, 1328, 1362, 1551, 1585
BciT130I CCWGG 6 cut(s) 182, 601, 617, 1814, 2138, 2640
BciVI GTATCC 1 cut(s) 2926
BclI TGATCA 2 cut(s) 873, 2392
BcnI CCSGG 1 cut(s) 537
BcoDI GTCTC 7 cut(s) 69, 190, 351, 445, 793, 1775, 2248
BfaI CTAG 6 cut(s) 1244, 1253, 1766, 1841, 1881, 2874
BfmI CTRYAG 2 cut(s) 687, 1831
BfrI CTTAAG 2 cut(s) 170, 1505
BfuAI ACCTGC 1 cut(s) 2467
BfuI GTATCC 1 cut(s) 2926
BglI GCCNNNNNGGC 1 cut(s) 1301
BglII AGATCT 1 cut(s) 2886
BlnI CCTAGG 1 cut(s) 1840
BmcAI AGTACT 1 cut(s) 897
Bme1390I CCNGG 7 cut(s) 182, 537, 601, 617, 1814, 2138, 2640
Bme18I GGWCC 3 cut(s) 13, 2089, 2360
BmeT110I CYCGRG 2 cut(s) 730, 761
BmgT120I GGNCC 4 cut(s) 13, 660, 2089, 2360
BmiI GGNNCC 4 cut(s) 400, 661, 758, 1838
BmrFI CCNGG 7 cut(s) 182, 537, 601, 617, 1814, 2138, 2640
BmsI GCATC 8 cut(s) 148, 1180, 1282, 1600, 1655, 1720, 1866, 2182
BpiI GAAGAC 1 cut(s) 1094
BpmI CTGGAG 3 cut(s) 172, 1697, 1799
Bpu10I CCTNAGC 2 cut(s) 1137, 2895
BpuEI CTTGAG 3 cut(s) 450, 620, 2097
BpuMI CCSGG 1 cut(s) 537
BsaAI YACGTR 1 cut(s) 826
BsaBI GATNNNNATC 1 cut(s) 2517
BsaHI GRCGYC 2 cut(s) 21, 623
BsaI GGTCTC 2 cut(s) 190, 2248
BsaJI CCNNGG 7 cut(s) 57, 600, 670, 867, 1182, 1840, 1997
BsaWI WCCGGW 2 cut(s) 332, 2017
BsaXI ACNNNNNCTCC 6 cut(s) 111, 141, 216, 246, 2689, 2719
Bse118I RCCGGY 2 cut(s) 1062, 2086
Bse1I ACTGG 3 cut(s) 315, 1975, 2965
Bse21I CCTNAGG 1 cut(s) 2736
Bse3DI GCAATG 2 cut(s) 1444, 2826
Bse8I GATNNNNATC 1 cut(s) 2517
BseBI CCWGG 6 cut(s) 182, 601, 617, 1814, 2138, 2640
BseDI CCNNGG 7 cut(s) 57, 600, 670, 867, 1182, 1840, 1997
BseGI GGATG 7 cut(s) 81, 976, 1615, 1852, 2293, 2635, 2952
BseJI GATNNNNATC 1 cut(s) 2517
BseMI GCAATG 2 cut(s) 1444, 2826
BseMII CTCAG 8 cut(s) 764, 1151, 1321, 1490, 1913, 2489, 2750, 2909
BseNI ACTGG 3 cut(s) 315, 1975, 2965
BseRI GAGGAG 6 cut(s) 77, 184, 224, 413, 2156, 2724
BseSI GKGCMC 1 cut(s) 1492
BseXI GCAGC 6 cut(s) 997, 1270, 1430, 1556, 1749, 2593
BsgI GTGCAG 2 cut(s) 1429, 2583
Bsh1236I CGCG 5 cut(s) 851, 1099, 1350, 1352, 2547
BshFI GGCC 9 cut(s) 294, 662, 669, 680, 716, 866, 1946, 2250, 2977
BsiHKAI GWGCWC 4 cut(s) 129, 423, 1037, 1753
BsiHKCI CYCGRG 2 cut(s) 730, 761
BsiSI CCGG 6 cut(s) 24, 333, 536, 1063, 2018, 2087
BsmAI GTCTC 7 cut(s) 69, 190, 351, 445, 793, 1775, 2248
BsmBI CGTCTC 1 cut(s) 445
BsmI GAATGC 1 cut(s) 2070
BsnI GGCC 9 cut(s) 294, 662, 669, 680, 716, 866, 1946, 2250, 2977
Bso31I GGTCTC 2 cut(s) 190, 2248
BsoBI CYCGRG 2 cut(s) 730, 761
Bsp1286I GDGCHC 5 cut(s) 129, 423, 1037, 1492, 1753
Bsp19I CCATGG 1 cut(s) 57
BspANI GGCC 9 cut(s) 294, 662, 669, 680, 716, 866, 1946, 2250, 2977
BspCNI CTCAG 8 cut(s) 763, 1150, 1320, 1489, 1912, 2490, 2749, 2908
BspFNI CGCG 5 cut(s) 851, 1099, 1350, 1352, 2547
BspHI TCATGA 2 cut(s) 279, 2515
BspLI GGNNCC 4 cut(s) 400, 661, 758, 1838
BspMI ACCTGC 1 cut(s) 2467
BspPI GGATC 9 cut(s) 277, 607, 963, 1111, 1253, 2009, 2052, 2513, 2520
BspQI GCTCTTC 2 cut(s) 182, 411
BspTI CTTAAG 2 cut(s) 170, 1505
BspTNI GGTCTC 2 cut(s) 190, 2248
BsrBI CCGCTC 1 cut(s) 307
BsrDI GCAATG 2 cut(s) 1444, 2826
BsrFI RCCGGY 2 cut(s) 1062, 2086
BsrI ACTGG 3 cut(s) 315, 1975, 2965
BssAI RCCGGY 2 cut(s) 1062, 2086
BssECI CCNNGG 7 cut(s) 57, 600, 670, 867, 1182, 1840, 1997
BssNI GRCGYC 2 cut(s) 21, 623
BssT1I CCWWGG 4 cut(s) 57, 670, 1840, 1997
Bst2UI CCWGG 6 cut(s) 182, 601, 617, 1814, 2138, 2640
Bst4CI ACNGT 8 cut(s) 572, 691, 745, 871, 2401, 2861, 2912, 2938
Bst6I CTCTTC 7 cut(s) 182, 411, 1194, 1380, 2323, 2595, 2823
BstACI GRCGYC 2 cut(s) 21, 623
BstAFI CTTAAG 2 cut(s) 170, 1505
BstAPI GCANNNNNTGC 4 cut(s) 574, 1443, 1496, 1730
BstBAI YACGTR 1 cut(s) 826
BstC8I GCNNGC 5 cut(s) 1497, 2070, 2506, 2538, 2975
BstDEI CTNAG 9 cut(s) 750, 1137, 1307, 1318, 1476, 1899, 2498, 2736, 2895
BstDSI CCRYGG 3 cut(s) 57, 867, 1182
BstF5I GGATG 7 cut(s) 81, 976, 1615, 1852, 2293, 2635, 2952
BstFNI CGCG 5 cut(s) 851, 1099, 1350, 1352, 2547
BstHHI GCGC 4 cut(s) 740, 887, 1099, 1352
BstMAI GTCTC 7 cut(s) 69, 190, 351, 445, 793, 1775, 2248
BstNI CCWGG 6 cut(s) 182, 601, 617, 1814, 2138, 2640
BstNSI RCATGY 3 cut(s) 2273, 2675, 2807
BstSCI CCNGG 7 cut(s) 180, 535, 599, 615, 1812, 2136, 2638
BstSFI CTRYAG 2 cut(s) 687, 1831
BstSLI GKGCMC 1 cut(s) 1492
BstUI CGCG 5 cut(s) 851, 1099, 1350, 1352, 2547
BstV1I GCAGC 6 cut(s) 997, 1270, 1430, 1556, 1749, 2593
BstV2I GAAGAC 1 cut(s) 1094
BstX2I RGATCY 4 cut(s) 1116, 1258, 2044, 2886
BstXI CCANNNNNNTGG 1 cut(s) 2617
BstYI RGATCY 4 cut(s) 1116, 1258, 2044, 2886
Bsu36I CCTNAGG 1 cut(s) 2736
BsuI GTATCC 1 cut(s) 2926
BsuRI GGCC 9 cut(s) 294, 662, 669, 680, 716, 866, 1946, 2250, 2977
BtgI CCRYGG 3 cut(s) 57, 867, 1182
BtgZI GCGATG 1 cut(s) 1331
BtsCI GGATG 7 cut(s) 81, 976, 1615, 1852, 2293, 2635, 2952
BtsI GCAGTG 2 cut(s) 136, 1490
BtsIMutI CAGTG 8 cut(s) 136, 224, 696, 1315, 1490, 2768, 2784, 2972
BveI ACCTGC 1 cut(s) 2467
Cac8I GCNNGC 5 cut(s) 1497, 2070, 2506, 2538, 2975
CciI TCATGA 2 cut(s) 279, 2515
CfoI GCGC 4 cut(s) 740, 887, 1099, 1352
Cfr10I RCCGGY 2 cut(s) 1062, 2086
Cfr13I GGNCC 4 cut(s) 13, 660, 2089, 2360
CseI GACGC 4 cut(s) 29, 612, 1088, 2015
Csp6I GTAC 5 cut(s) 494, 896, 2543, 2556, 2801
CspCI CAANNNNNGTGG 2 cut(s) 706, 741
CviQI GTAC 5 cut(s) 494, 896, 2543, 2556, 2801
DdeI CTNAG 9 cut(s) 750, 1137, 1307, 1318, 1476, 1899, 2498, 2736, 2895
DraIII CACNNNGTG 1 cut(s) 1310
EaeI YGGCCR 4 cut(s) 678, 714, 864, 2248
Eam1104I CTCTTC 7 cut(s) 182, 411, 1194, 1380, 2323, 2595, 2823
EarI CTCTTC 7 cut(s) 182, 411, 1194, 1380, 2323, 2595, 2823
EciI GGCGGA 1 cut(s) 203
Ecl136II GAGCTC 4 cut(s) 127, 421, 1035, 1751
Eco130I CCWWGG 4 cut(s) 57, 670, 1840, 1997
Eco24I GRGCYC 4 cut(s) 129, 423, 1037, 1753
Eco31I GGTCTC 2 cut(s) 190, 2248
Eco32I GATATC 1 cut(s) 2134
Eco47I GGWCC 3 cut(s) 13, 2089, 2360
Eco53kI GAGCTC 4 cut(s) 127, 421, 1035, 1751
Eco57I CTGAAG 6 cut(s) 376, 967, 1417, 2304, 2619, 2808
Eco81I CCTNAGG 1 cut(s) 2736
Eco88I CYCGRG 2 cut(s) 730, 761
EcoICRI GAGCTC 4 cut(s) 127, 421, 1035, 1751
EcoRI GAATTC 1 cut(s) 2582
EcoRII CCWGG 6 cut(s) 180, 599, 615, 1812, 2136, 2638
EcoRV GATATC 1 cut(s) 2134
EcoT14I CCWWGG 4 cut(s) 57, 670, 1840, 1997
EcoT22I ATGCAT 1 cut(s) 1648
EcoT38I GRGCYC 4 cut(s) 129, 423, 1037, 1753
ErhI CCWWGG 4 cut(s) 57, 670, 1840, 1997
Esp3I CGTCTC 1 cut(s) 445
FalI AAGNNNNNCTT 2 cut(s) 1703, 1735
FauI CCCGC 2 cut(s) 265, 312
FauNDI CATATG 1 cut(s) 1400
FbaI TGATCA 2 cut(s) 873, 2392
FblI GTMKAC 2 cut(s) 686, 1866
FokI GGATG 7 cut(s) 68, 983, 1622, 1859, 2300, 2622, 2959
FriOI GRGCYC 4 cut(s) 129, 423, 1037, 1753
FspBI CTAG 6 cut(s) 1244, 1253, 1766, 1841, 1881, 2874
GlaI GCGC 4 cut(s) 739, 886, 1098, 1351
GsuI CTGGAG 3 cut(s) 172, 1697, 1799
HaeIII GGCC 9 cut(s) 294, 662, 669, 680, 716, 866, 1946, 2250, 2977
HapII CCGG 6 cut(s) 24, 333, 536, 1063, 2018, 2087
HgaI GACGC 4 cut(s) 29, 612, 1088, 2015
HhaI GCGC 4 cut(s) 740, 887, 1099, 1352
Hin1I GRCGYC 2 cut(s) 21, 623
Hin6I GCGC 4 cut(s) 738, 885, 1097, 1350
HinP1I GCGC 4 cut(s) 738, 885, 1097, 1350
HincII GTYRAC 6 cut(s) 727, 941, 1867, 1924, 2857, 2941
HindII GTYRAC 6 cut(s) 727, 941, 1867, 1924, 2857, 2941
HindIII AAGCTT 1 cut(s) 1938
HpaI GTTAAC 2 cut(s) 2857, 2941
HpaII CCGG 6 cut(s) 24, 333, 536, 1063, 2018, 2087
HphI GGTGA 7 cut(s) 145, 241, 793, 883, 1960, 2399, 2924
Hpy99I CGWCG 5 cut(s) 371, 668, 914, 1573, 1871
HpyAV CCTTC 5 cut(s) 140, 918, 1923, 2176, 2495
HpyCH4III ACNGT 8 cut(s) 572, 691, 745, 871, 2401, 2861, 2912, 2938
HpyCH4IV ACGT 4 cut(s) 438, 459, 825, 1360
HpyF3I CTNAG 9 cut(s) 750, 1137, 1307, 1318, 1476, 1899, 2498, 2736, 2895
HpySE526I ACGT 4 cut(s) 438, 459, 825, 1360
Hsp92I GRCGYC 2 cut(s) 21, 623
HspAI GCGC 4 cut(s) 738, 885, 1097, 1350
Ksp22I TGATCA 2 cut(s) 873, 2392
KspAI GTTAAC 2 cut(s) 2857, 2941
LguI GCTCTTC 2 cut(s) 182, 411
Lsp1109I GCAGC 6 cut(s) 997, 1270, 1430, 1556, 1749, 2593
LweI GCATC 8 cut(s) 148, 1180, 1282, 1600, 1655, 1720, 1866, 2182
MaeI CTAG 6 cut(s) 1244, 1253, 1766, 1841, 1881, 2874
MaeII ACGT 4 cut(s) 438, 459, 825, 1360
MaeIII GTNAC 4 cut(s) 460, 781, 1168, 2011
MbiI CCGCTC 1 cut(s) 307
MflI RGATCY 4 cut(s) 1116, 1258, 2044, 2886
MhlI GDGCHC 5 cut(s) 129, 423, 1037, 1492, 1753
MlsI TGGCCA 3 cut(s) 680, 716, 866
MluNI TGGCCA 3 cut(s) 680, 716, 866
MlyI GAGTC 5 cut(s) 71, 795, 863, 943, 2805
MmeI TCCRAC 7 cut(s) 426, 480, 810, 1551, 1687, 2687, 2736
Mox20I TGGCCA 3 cut(s) 680, 716, 866
Mph1103I ATGCAT 1 cut(s) 1648
MroXI GAANNNNTTC 1 cut(s) 2832
MscI TGGCCA 3 cut(s) 680, 716, 866
Msp20I TGGCCA 3 cut(s) 680, 716, 866
MspA1I CMGCKG 3 cut(s) 10, 396, 409
MspCI CTTAAG 2 cut(s) 170, 1505
MspI CCGG 6 cut(s) 24, 333, 536, 1063, 2018, 2087
MspR9I CCNGG 7 cut(s) 182, 537, 601, 617, 1814, 2138, 2640
Mva1269I GAATGC 1 cut(s) 2070
MvaI CCWGG 6 cut(s) 182, 601, 617, 1814, 2138, 2640
MvnI CGCG 5 cut(s) 851, 1099, 1350, 1352, 2547
NciI CCSGG 1 cut(s) 537
NcoI CCATGG 1 cut(s) 57
NdeI CATATG 1 cut(s) 1400
NlaIV GGNNCC 4 cut(s) 400, 661, 758, 1838
NmuCI GTSAC 2 cut(s) 460, 781
NsiI ATGCAT 1 cut(s) 1648
NspI RCATGY 3 cut(s) 2273, 2675, 2807
PaeR7I CTCGAG 1 cut(s) 761
PagI TCATGA 2 cut(s) 279, 2515
PciI ACATGT 2 cut(s) 2269, 2671
PciSI GCTCTTC 2 cut(s) 182, 411
PcsI WCGNNNNNNNCGW 2 cut(s) 1190, 1734
PctI GAATGC 1 cut(s) 2070
PdmI GAANNNNTTC 1 cut(s) 2832
PflMI CCANNNNNTGG 4 cut(s) 481, 1606, 1675, 1819
PfoI TCCNGGA 1 cut(s) 2136
PleI GAGTC 5 cut(s) 70, 795, 862, 942, 2805
PpsI GAGTC 5 cut(s) 70, 795, 862, 942, 2805
Ppu21I YACGTR 1 cut(s) 826
PscI ACATGT 2 cut(s) 2269, 2671
PshBI ATTAAT 1 cut(s) 2727
PsiI TTATAA 2 cut(s) 770, 2159
Psp124BI GAGCTC 4 cut(s) 129, 423, 1037, 1753
Psp6I CCWGG 6 cut(s) 180, 599, 615, 1812, 2136, 2638
PspGI CCWGG 6 cut(s) 180, 599, 615, 1812, 2136, 2638
PspN4I GGNNCC 4 cut(s) 400, 661, 758, 1838
PspPI GGNCC 4 cut(s) 13, 660, 2089, 2360
PspXI VCTCGAGB 1 cut(s) 761
PsuI RGATCY 4 cut(s) 1116, 1258, 2044, 2886
PvuII CAGCTG 2 cut(s) 10, 409
RsaI GTAC 5 cut(s) 495, 897, 2544, 2557, 2802
RsaNI GTAC 5 cut(s) 494, 896, 2543, 2556, 2801
SacI GAGCTC 4 cut(s) 129, 423, 1037, 1753
SalI GTCGAC 1 cut(s) 1865
SapI GCTCTTC 2 cut(s) 182, 411
Sau96I GGNCC 4 cut(s) 13, 660, 2089, 2360
ScaI AGTACT 1 cut(s) 897
SchI GAGTC 5 cut(s) 71, 795, 863, 943, 2805
ScrFI CCNGG 7 cut(s) 182, 537, 601, 617, 1814, 2138, 2640
SduI GDGCHC 5 cut(s) 129, 423, 1037, 1492, 1753
SfaNI GCATC 8 cut(s) 148, 1180, 1282, 1600, 1655, 1720, 1866, 2182
SfcI CTRYAG 2 cut(s) 687, 1831
Sfr274I CTCGAG 1 cut(s) 761
SinI GGWCC 3 cut(s) 13, 2089, 2360
SlaI CTCGAG 1 cut(s) 761
SmlI CTYRAG 6 cut(s) 170, 465, 635, 761, 1505, 2112
SmoI CTYRAG 6 cut(s) 170, 465, 635, 761, 1505, 2112
SspI AATATT 1 cut(s) 2471
SspMI CTAG 6 cut(s) 1244, 1253, 1766, 1841, 1881, 2874
SstI GAGCTC 4 cut(s) 129, 423, 1037, 1753
StyD4I CCNGG 7 cut(s) 180, 535, 599, 615, 1812, 2136, 2638
StyI CCWWGG 4 cut(s) 57, 670, 1840, 1997
TaaI ACNGT 8 cut(s) 572, 691, 745, 871, 2401, 2861, 2912, 2938
TaiI ACGT 4 cut(s) 441, 462, 828, 1363
TatI WGTACW 1 cut(s) 895
TauI GCSGC 8 cut(s) 219, 399, 608, 611, 623, 1133, 1136, 2253
TscAI CASTG 8 cut(s) 143, 231, 696, 1315, 1497, 2775, 2791, 2972
TseFI GTSAC 2 cut(s) 460, 781
TseI GCWGC 6 cut(s) 1010, 1283, 1443, 1544, 1762, 2606
Tsp45I GTSAC 2 cut(s) 460, 781
TspDTI ATGAA 3 cut(s) 17, 478, 2595
TspGWI ACGGA 1 cut(s) 1095
TspRI CASTG 8 cut(s) 143, 231, 696, 1315, 1497, 2775, 2791, 2972
Van91I CCANNNNNTGG 4 cut(s) 481, 1606, 1675, 1819
Vha464I CTTAAG 2 cut(s) 170, 1505
VpaK11BI GGWCC 3 cut(s) 13, 2089, 2360
VspI ATTAAT 1 cut(s) 2727
XapI RAATTY 6 cut(s) 351, 964, 1769, 2199, 2218, 2582
XbaI TCTAGA 1 cut(s) 1243
XceI RCATGY 3 cut(s) 2273, 2675, 2807
XcmI CCANNNNNNNNNTGG 1 cut(s) 688
XhoI CTCGAG 1 cut(s) 761
XmaJI CCTAGG 1 cut(s) 1840
XmiI GTMKAC 2 cut(s) 686, 1866
XmnI GAANNNNTTC 1 cut(s) 2832
XspI CTAG 6 cut(s) 1244, 1253, 1766, 1841, 1881, 2874
ZrmI AGTACT 1 cut(s) 897
Zsp2I ATGCAT 1 cut(s) 1648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.