pycom290g00020

Deoxyribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_290
N/A
Physical Location & Seq
Forward (+)
28966 .. 29946
981 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 495 bp
ATGTGTAACTGTGTAAGGTTTCCGCTGCAGGTTAATGTATTCAGGCAACAACTTGAACTTGCGAAAGAGTTAAAAAAGCCAGCATCCATCCATTGTGTCCGTGCTTTTGGTGACCTTCTTCAGATAATGAAACCTTCAGGGCCTTTTCCTGCTGGCGTGATTCTTCATTCTTACCTAGGTTCTGCTGAGATGGTTCCTGAATTTGCCATCTTGGTGCTTACTTTTCATTCTCTGGATTTCTCATGTCCACGAAATGGGGTGCCTTCTGAAAAGATATTACTGGAGATAGATGCACCTGATGCATTACCAAAATCTGATATAGGTTCTCTGCATCTGATTGAAGGAGATGCTTCCCTCCTTGAAGAGCTTCAGTTGCAAGAAATTTCTTCAGCTCTGACGGCTAGTGCAAGAGACACTTCAACGCTTACAAAAGCAGCACTAAATCACCCGGCAAGTATTCACAACATGTACTCATCTATGTCGCATCTATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TatD_DNase

Protein Analysis

165

Amino Acids

17.87

Weight (kDa)

5.74

Isoelectric Point (pI)

55.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TatD_DNase PF01026 9 - 108 1.3e-16 TatD related DNase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 19
AccB1I GGYRCC 1 cut(s) 259
AccB7I CCANNNNNTGG 1 cut(s) 254
AciI CCGC 1 cut(s) 23
AcsI RAATTY 2 cut(s) 200, 381
AcuI CTGAAG 4 cut(s) 104, 120, 353, 372
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 1 cut(s) 254
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 4 cut(s) 56, 341, 362, 420
AluBI AGCT 2 cut(s) 367, 392
AluI AGCT 2 cut(s) 367, 392
Alw26I GTCTC 1 cut(s) 405
AoxI GGCC 1 cut(s) 140
ApeKI GCWGC 2 cut(s) 25, 434
ApoI RAATTY 2 cut(s) 200, 381
Asp700I GAANNNNTTC 1 cut(s) 366
AspA2I CCTAGG 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 140
AsuC2I CCSGG 1 cut(s) 449
AsuHPI GGTGA 2 cut(s) 122, 437
AvrII CCTAGG 1 cut(s) 175
BanI GGYRCC 1 cut(s) 259
BbvI GCAGC 2 cut(s) 12, 446
BccI CCATC 3 cut(s) 95, 184, 215
BceAI ACGGC 1 cut(s) 414
BcnI CCSGG 1 cut(s) 449
BcoDI GTCTC 1 cut(s) 405
BfaI CTAG 3 cut(s) 176, 402, 493
BfmI CTRYAG 1 cut(s) 26
BfuAI ACCTGC 1 cut(s) 19
BisI GCNGC 2 cut(s) 26, 435
BlnI CCTAGG 1 cut(s) 175
BlsI GCNGC 2 cut(s) 27, 436
Bme1390I CCNGG 1 cut(s) 449
BmgT120I GGNCC 1 cut(s) 140
BmiI GGNNCC 2 cut(s) 195, 261
BmrFI CCNGG 1 cut(s) 449
BmsI GCATC 5 cut(s) 92, 280, 289, 337, 340
BpmI CTGGAG 1 cut(s) 302
BpuMI CCSGG 1 cut(s) 449
BsaJI CCNNGG 1 cut(s) 175
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse1I ACTGG 1 cut(s) 285
BseDI CCNNGG 1 cut(s) 175
BseGI GGATG 2 cut(s) 83, 87
BseLI CCNNNNNNNGG 1 cut(s) 254
BseMII CTCAG 1 cut(s) 177
BseNI ACTGG 1 cut(s) 285
BseXI GCAGC 2 cut(s) 12, 446
BshFI GGCC 1 cut(s) 142
BshNI GGYRCC 1 cut(s) 259
BsiSI CCGG 1 cut(s) 449
BslI CCNNNNNNNGG 1 cut(s) 254
BsmAI GTCTC 1 cut(s) 405
BsnI GGCC 1 cut(s) 142
BspACI CCGC 1 cut(s) 23
BspANI GGCC 1 cut(s) 142
BspCNI CTCAG 1 cut(s) 178
BspLI GGNNCC 2 cut(s) 195, 261
BspMAI CTGCAG 1 cut(s) 30
BspMI ACCTGC 1 cut(s) 19
BspQI GCTCTTC 1 cut(s) 357
BspT107I GGYRCC 1 cut(s) 259
BsrI ACTGG 1 cut(s) 285
BssECI CCNNGG 1 cut(s) 175
BssT1I CCWWGG 1 cut(s) 175
Bst4CI ACNGT 1 cut(s) 11
Bst6I CTCTTC 1 cut(s) 357
BstAPI GCANNNNNTGC 1 cut(s) 299
BstC8I GCNNGC 2 cut(s) 81, 154
BstDEI CTNAG 1 cut(s) 186
BstEII GGTNACC 1 cut(s) 110
BstF5I GGATG 2 cut(s) 83, 87
BstMAI GTCTC 1 cut(s) 405
BstMWI GCNNNNNNNGC 3 cut(s) 299, 373, 398
BstNSI RCATGY 1 cut(s) 469
BstPI GGTNACC 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 447
BstSFI CTRYAG 1 cut(s) 26
BstV1I GCAGC 2 cut(s) 12, 446
BsuRI GGCC 1 cut(s) 142
BtsCI GGATG 2 cut(s) 83, 87
BveI ACCTGC 1 cut(s) 19
Cac8I GCNNGC 2 cut(s) 81, 154
Cfr13I GGNCC 1 cut(s) 140
Csp6I GTAC 1 cut(s) 469
CviAII CATG 2 cut(s) 243, 466
CviJI RGCY 5 cut(s) 79, 142, 367, 392, 401
CviKI_1 RGCY 5 cut(s) 79, 142, 367, 392, 401
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 1 cut(s) 186
Eam1104I CTCTTC 1 cut(s) 357
EarI CTCTTC 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 175
Eco57I CTGAAG 4 cut(s) 104, 120, 353, 372
Eco91I GGTNACC 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 140
EcoO65I GGTNACC 1 cut(s) 110
EcoT14I CCWWGG 1 cut(s) 175
EcoT22I ATGCAT 1 cut(s) 304
ErhI CCWWGG 1 cut(s) 175
FaeI CATG 2 cut(s) 246, 469
FaiI YATR 5 cut(s) 244, 320, 467, 479, 490
FalI AAGNNNNNCTT 2 cut(s) 400, 432
FatI CATG 2 cut(s) 242, 465
Fnu4HI GCNGC 2 cut(s) 26, 435
FokI GGATG 2 cut(s) 70, 74
Fsp4HI GCNGC 2 cut(s) 26, 435
FspBI CTAG 3 cut(s) 176, 402, 493
GluI GCNGC 2 cut(s) 26, 435
GsuI CTGGAG 1 cut(s) 302
HaeIII GGCC 1 cut(s) 142
HapII CCGG 1 cut(s) 449
Hin1II CATG 2 cut(s) 246, 469
HinfI GANTC 1 cut(s) 160
HpaII CCGG 1 cut(s) 449
HphI GGTGA 2 cut(s) 122, 437
Hpy166II GTNNAC 1 cut(s) 248
Hpy188I TCNGA 5 cut(s) 123, 268, 316, 336, 396
Hpy188III TCNNGA 2 cut(s) 197, 233
Hpy8I GTNNAC 1 cut(s) 248
HpyAV CCTTC 4 cut(s) 125, 144, 273, 335
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4V TGCA 6 cut(s) 28, 293, 302, 331, 376, 407
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 373, 398
HpyF3I CTNAG 1 cut(s) 186
Hsp92II CATG 2 cut(s) 246, 469
LguI GCTCTTC 1 cut(s) 357
Lsp1109I GCAGC 2 cut(s) 12, 446
LweI GCATC 5 cut(s) 92, 280, 289, 337, 340
MaeI CTAG 3 cut(s) 176, 402, 493
MaeIII GTNAC 2 cut(s) 5, 110
MboII GAAGA 4 cut(s) 110, 155, 374, 378
MluCI AATT 2 cut(s) 200, 381
MnlI CCTC 1 cut(s) 365
Mph1103I ATGCAT 1 cut(s) 304
MroXI GAANNNNTTC 1 cut(s) 366
MseI TTAA 2 cut(s) 33, 71
MslI CAYNNNNRTG 1 cut(s) 212
MspA1I CMGCKG 1 cut(s) 25
MspI CCGG 1 cut(s) 449
MspR9I CCNGG 1 cut(s) 449
MwoI GCNNNNNNNGC 3 cut(s) 299, 373, 398
NciI CCSGG 1 cut(s) 449
NlaIII CATG 2 cut(s) 246, 469
NlaIV GGNNCC 2 cut(s) 195, 261
NmuCI GTSAC 1 cut(s) 110
NsiI ATGCAT 1 cut(s) 304
NspI RCATGY 1 cut(s) 469
PciI ACATGT 1 cut(s) 465
PciSI GCTCTTC 1 cut(s) 357
PdmI GAANNNNTTC 1 cut(s) 366
PfeI GAWTC 1 cut(s) 160
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 2 cut(s) 27, 436
PscI ACATGT 1 cut(s) 465
PspEI GGTNACC 1 cut(s) 110
PspN4I GGNNCC 2 cut(s) 195, 261
PspPI GGNCC 1 cut(s) 140
PstI CTGCAG 1 cut(s) 30
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
RseI CAYNNNNRTG 1 cut(s) 212
SapI GCTCTTC 1 cut(s) 357
SaqAI TTAA 2 cut(s) 33, 71
SatI GCNGC 2 cut(s) 26, 435
Sau96I GGNCC 1 cut(s) 140
ScrFI CCNGG 1 cut(s) 449
SfaNI GCATC 5 cut(s) 92, 280, 289, 337, 340
SfcI CTRYAG 1 cut(s) 26
SmiMI CAYNNNNRTG 1 cut(s) 212
Sse9I AATT 2 cut(s) 200, 381
SsiI CCGC 1 cut(s) 23
SspMI CTAG 3 cut(s) 176, 402, 493
StyD4I CCNGG 1 cut(s) 447
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 1 cut(s) 11
TasI AATT 2 cut(s) 200, 381
TatI WGTACW 1 cut(s) 468
TfiI GAWTC 1 cut(s) 160
Tru1I TTAA 2 cut(s) 33, 71
Tru9I TTAA 2 cut(s) 33, 71
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 2 cut(s) 25, 434
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 3 cut(s) 143, 155, 215
TspGWI ACGGA 1 cut(s) 89
Van91I CCANNNNNTGG 1 cut(s) 254
XapI RAATTY 2 cut(s) 200, 381
XceI RCATGY 1 cut(s) 469
XmaJI CCTAGG 1 cut(s) 175
XmnI GAANNNNTTC 1 cut(s) 366
XspI CTAG 3 cut(s) 176, 402, 493
Zsp2I ATGCAT 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.