Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

SuperScaffold_520

Orthogroup ID

N/A

Physical Location & Seq

Genomic Coordinates Forward (+)

217842 .. 220958

Gene Structure 3117 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2310 bp

ATGGAAGGAAGCAAGGTATCCCAAAGACCAGGATTAAGGTGCCCAGGCGCATATCCAATAAAAGATAGCATCCGTTGCAACCAAGGGTGCAAAGGCAACTTCACCCCTAAAAGGGCAATTCTTCATGATGTACCAAACGCATTCCTACACTACAGGGGATACTACATGACTGCCTTAGGGCCTCAAGCTATTTTTCACTATCTAACAAGTTTTTCTCTAAAAGGTCCGCTGAGGAAGAACCAATATTGTCTAAGGTTTGACAATTGCGAAAATCATCCAACGTTTCTTTTGTACAAGACTCCAACAAAGGCCCTGAAGACTCCAACATTGCAAGACTTCCAGACTCTGACATCTACAACAAACAGAAACAAACTCTATCACTACATGAAAGATCGAAACATAAGGAAGTTCATAGGACATGAACACCCAAACAATTCAACAAGAATGAAAACATGCGATCTAGCGGAGAAGACTACCAACCTGAAGATGGTGCAAAGAAATGCCGGAATCCCTCTCAACTATAGAACACTCTGTCTTCCAAAATTACTACAAAATGAAAGTTCAATAGGACCAAATAAATTCAAAATTCAAATTCAAACCATGAGAAAGCCCGACATGGAAAGTCTTCAAACTTCCACACAAGCTAGGGATAAATTAAATTATAAAAAAAAAAAAAAAAAAAAAAAAGGAGGGGGAACACAGCAACTTCATCAATGGAAGACAAATTTCAAATCTCAAAAGGTGCCAACAAAAGCTTCAACAAATGAAGGGCAACTACAAATGTCAAAAGCCTCACACACTCTTGATCAAGATAGTGTGAAGCAAAACCAATTTATGGTGACCAACACAAAGCTTCACCTACAAAGCTTCAACCAACAATGCTTCAACTACAAAGCTTCAACCACAAAGCTTCACCTACAAAGCTTCAACTCTCAACCACAAAGCTTCACCTACAAAGCTTCAACCTTCAACCACAAAGCTTCAACTACCAAGCTTCACCTACAAAGCTTCACCAAATGGAGAGCAACTACAAATTTAAATCAAGTTTGGTGTCCTAAAATAGCTTCAACCATTCAAGCTATATACCCAAACAAAAACCCACAACCAATACACCTTACCTATTTACTTATATATGGTGAACAAAAGTACAAGAAATCCACCAGTAATGAAGGTGCTACAAAAACTGGAATCTCCCCCAAACTAGAAATCCAACCCAAGCAGCCTCTTTCTCTTTCTCCCTCTTCCGTCTCCTTTAATCTATCAAATTTCTGCAACTTCTTCTCTTCTTCAATGGAGCTGAATTTTGGACATCTTATAGTCCTTGAGCAGATGAACAACTTTCAAGAAGGAATTGTTTCCTTCTGCAGACTTCAAAGCTGCCGTAGTGTTTCGAAGATCTGGAAATATTTAACCGTTAGCTTAAAGTATGTCGATCTGAACAAGAGAAAATGGAATTTCGAAGCCCACAACGGCATACCTGAATTTCCAGAGTTGTACTGCTCCGATGGATCTCAAATTTGGATATGTTGTAGTTACAAGTTAAGGAATAACTTTCATGAAGACAACTTTGTGATTCATACTATAGATGATGGTGTTATATTGAAAATCTACCGCTCCCACTACATCGATTCCCTCTTCGACTTCCCCAGCTCCAACACCGCGTCAGAGCAGGACCTCATCCACATTTCCTTCAACATGACTCTGCAACAACTCAGGTTGTTAGTGAAAATGAAAAGCAATTCCACCAAAGAAGGGAAAAGCAAGACAAAAGAAAAGCTAAAAAATGAGAAAAAGCAAGGGAAATGGGGAAGAAGACATGGGCAAAGGGATGAGACCTTGGGTCTTATTTTTTTAGGAAGGTGTGACACAAAAATAAAGCTTCGTTACTTTGAGACTTGGGGGACTCCTACCATATACCACTACACCTTGGTACTCGAAAGTTTTGCGACTACTGACACTACTTCAACCTACAGGATCCAAAAACCCATGAAGTTTCAACATGCAAACTTTAACAAAATAAAAAATTCAAAGAACTCGGTGAAGGATGCCTTAGTGTATTTAACACAACACTACAAATACGTGCATACACATGAATCAAAACAAACAAACAAGAGGGCGCCTTCACAAAGGTTGCTCAGGAGAAGTCTCAGCACTCGGCAAAGCCCCAGAAATAGGAGGCACCGGAGGGTGATTATTTGGAGCTTCACTGGTGATTTGGAGCTTCAGTATGTGGTACAACCCCAGAGGACGAAGGCAATAAATTCCTTTCGAACAAACTCACAAACCTCTGATCGATCATTTAAAATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0008150 GO:0009505 GO:0010119 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030312 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050789 GO:0050790 GO:0050794 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

770

Amino Acids

89.37

Weight (kDa)

9.99

Isoelectric Point (pI)

42.06

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	663
AccB1I	GGYRCC	4 cut(s)	39, 742, 2116, 2178
AccB7I	CCANNNNNTGG	1 cut(s)	835
AccBSI	CCGCTC	1 cut(s)	1614
AccII	CGCG	1 cut(s)	1661
AciI	CCGC	4 cut(s)	227, 464, 1612, 1659
AclI	AACGTT	1 cut(s)	281
AclWI	GGATC	3 cut(s)	1516, 1969, 1982
AcuI	CTGAAG	3 cut(s)	335, 503, 2207
AcyI	GRCGYC	1 cut(s)	2117
AfaI	GTAC	6 cut(s)	132, 293, 1148, 1496, 1932, 2235
AfiI	CCNNNNNNNGG	6 cut(s)	111, 112, 487, 835, 1752, 2172
AhdI	GACNNNNNGTC	1 cut(s)	1839
AjnI	CCWGG	2 cut(s)	28, 43
AjuI	GAANNNNNNNTTGG	2 cut(s)	739, 771
Alw26I	GTCTC	4 cut(s)	1252, 1826, 1886, 2150
AlwI	GGATC	3 cut(s)	1516, 1969, 1982
AlwNI	CAGNNNCTG	1 cut(s)	346
AoxI	GGCC	2 cut(s)	179, 309
ApeKI	GCWGC	2 cut(s)	1219, 1377
Asp700I	GAANNNNTTC	2 cut(s)	624, 1354
AspLEI	GCGC	2 cut(s)	50, 2119
AspS9I	GGNCC	5 cut(s)	179, 224, 310, 569, 1672
AsuII	TTCGAA	3 cut(s)	1391, 1458, 2269
AvaII	GGWCC	3 cut(s)	224, 569, 1672
AxyI	CCTNAGG	1 cut(s)	175
BaeGI	GKGCMC	1 cut(s)	44
BamHI	GGATCC	1 cut(s)	1974
BanI	GGYRCC	4 cut(s)	39, 742, 2116, 2178
BarI	GAAGNNNNNNTAC	2 cut(s)	759, 791
BbsI	GAAGAC	7 cut(s)	323, 476, 527, 617, 725, 1566, 1819
BbvCI	CCTCAGC	1 cut(s)	230
BbvI	GCAGC	2 cut(s)	1231, 1364
BccI	CCATC	3 cut(s)	481, 1499, 1583
BceAI	ACGGC	2 cut(s)	1365, 1486
BcgI	CGANNNNNNTGC	2 cut(s)	1925, 1959
BciT130I	CCWGG	2 cut(s)	30, 45
BciVI	GTATCC	2 cut(s)	28, 152
BclI	TGATCA	1 cut(s)	805
BcoDI	GTCTC	4 cut(s)	1252, 1826, 1886, 2150
BfaI	CTAG	3 cut(s)	461, 645, 1202
BfmI	CTRYAG	5 cut(s)	151, 520, 1363, 1581, 1969
BfoI	RGCGCY	1 cut(s)	2120
BfuI	GTATCC	2 cut(s)	28, 152
BglII	AGATCT	1 cut(s)	1395
BisI	GCNGC	2 cut(s)	1220, 1378
BlsI	GCNGC	2 cut(s)	1221, 1379
Bme1390I	CCNGG	2 cut(s)	30, 45
Bme18I	GGWCC	3 cut(s)	224, 569, 1672
BmeRI	GACNNNNNGTC	1 cut(s)	1839
BmgT120I	GGNCC	5 cut(s)	179, 224, 310, 569, 1672
BmiI	GGNNCC	5 cut(s)	41, 744, 1976, 2118, 2180
BmrFI	CCNGG	2 cut(s)	30, 45
BmsI	GCATC	2 cut(s)	78, 2035
BpiI	GAAGAC	7 cut(s)	323, 476, 527, 617, 725, 1566, 1819
Bpu10I	CCTNAGC	2 cut(s)	230, 2135
Bpu14I	TTCGAA	3 cut(s)	1391, 1458, 2269
BpuEI	CTTGAG	2 cut(s)	168, 1343
Bsa29I	ATCGAT	2 cut(s)	1626, 2293
BsaAI	YACGTR	1 cut(s)	2080
BsaHI	GRCGYC	1 cut(s)	2117
BsaI	GGTCTC	1 cut(s)	1826
BsaJI	CCNNGG	4 cut(s)	43, 82, 1836, 1926
BsaWI	WCCGGW	1 cut(s)	2181
BsaXI	ACNNNNNCTCC	2 cut(s)	2210, 2240
Bsc4I	CCNNNNNNNGG	6 cut(s)	111, 112, 487, 835, 1752, 2172
Bse1I	ACTGG	3 cut(s)	1161, 1189, 2212
Bse21I	CCTNAGG	1 cut(s)	175
Bse3DI	GCAATG	1 cut(s)	326
BseBI	CCWGG	2 cut(s)	30, 45
BseCI	ATCGAT	2 cut(s)	1626, 2293
BseDI	CCNNGG	4 cut(s)	43, 82, 1836, 1926
BseGI	GGATG	5 cut(s)	69, 274, 1677, 1834, 2050
BseLI	CCNNNNNNNGG	6 cut(s)	111, 112, 487, 835, 1752, 2172
BseMI	GCAATG	1 cut(s)	326
BseMII	CTCAG	4 cut(s)	221, 1726, 2149, 2161
BseNI	ACTGG	3 cut(s)	1161, 1189, 2212
BseSI	GKGCMC	1 cut(s)	44
BseXI	GCAGC	2 cut(s)	1231, 1364
BseYI	CCCAGC	1 cut(s)	1646
Bsh1236I	CGCG	1 cut(s)	1661
BshFI	GGCC	2 cut(s)	181, 311
BshNI	GGYRCC	4 cut(s)	39, 742, 2116, 2178
BshVI	ATCGAT	2 cut(s)	1626, 2293
BsiSI	CCGG	2 cut(s)	504, 2182
BslFI	GGGAC	1 cut(s)	1915
BslI	CCNNNNNNNGG	6 cut(s)	111, 112, 487, 835, 1752, 2172
BsmAI	GTCTC	4 cut(s)	1252, 1826, 1886, 2150
BsmBI	CGTCTC	1 cut(s)	1252
BsmFI	GGGAC	1 cut(s)	1915
BsmI	GAATGC	1 cut(s)	140
BsnI	GGCC	2 cut(s)	181, 311
Bso31I	GGTCTC	1 cut(s)	1826
Bsp119I	TTCGAA	3 cut(s)	1391, 1458, 2269
Bsp1286I	GDGCHC	1 cut(s)	44
Bsp1407I	TGTACA	1 cut(s)	291
Bsp143I	GATC	9 cut(s)	391, 457, 805, 1395, 1432, 1508, 1974, 2290, 2294
BspACI	CCGC	4 cut(s)	227, 464, 1612, 1659
BspANI	GGCC	2 cut(s)	181, 311
BspCNI	CTCAG	4 cut(s)	222, 1725, 2148, 2160
BspDI	ATCGAT	2 cut(s)	1626, 2293
BspFNI	CGCG	1 cut(s)	1661
BspHI	TCATGA	2 cut(s)	124, 1555
BspLI	GGNNCC	5 cut(s)	41, 744, 1976, 2118, 2180
BspMAI	CTGCAG	1 cut(s)	1367
BspPI	GGATC	3 cut(s)	1516, 1969, 1982
BspT104I	TTCGAA	3 cut(s)	1391, 1458, 2269
BspT107I	GGYRCC	4 cut(s)	39, 742, 2116, 2178
BspTNI	GGTCTC	1 cut(s)	1826
BsrBI	CCGCTC	1 cut(s)	1614
BsrDI	GCAATG	1 cut(s)	326
BsrGI	TGTACA	1 cut(s)	291
BsrI	ACTGG	3 cut(s)	1161, 1189, 2212
BssECI	CCNNGG	4 cut(s)	43, 82, 1836, 1926
BssMI	GATC	9 cut(s)	391, 457, 805, 1395, 1432, 1508, 1974, 2290, 2294
BssNI	GRCGYC	1 cut(s)	2117
BssT1I	CCWWGG	3 cut(s)	82, 1836, 1926
Bst2UI	CCWGG	2 cut(s)	30, 45
Bst4CI	ACNGT	1 cut(s)	1414
Bst6I	CTCTTC	3 cut(s)	1246, 1288, 1640
BstACI	GRCGYC	1 cut(s)	2117
BstAPI	GCANNNNNTGC	1 cut(s)	75
BstAUI	TGTACA	1 cut(s)	291
BstBAI	YACGTR	1 cut(s)	2080
BstBI	TTCGAA	3 cut(s)	1391, 1458, 2269
BstDEI	CTNAG	7 cut(s)	175, 230, 251, 1712, 2050, 2135, 2147
BstEII	GGTNACC	1 cut(s)	838
BstF5I	GGATG	5 cut(s)	69, 274, 1677, 1834, 2050
BstFNI	CGCG	1 cut(s)	1661
BstH2I	RGCGCY	1 cut(s)	2120
BstHHI	GCGC	2 cut(s)	50, 2119
BstKTI	GATC	9 cut(s)	394, 460, 808, 1398, 1435, 1511, 1977, 2293, 2297
BstMAI	GTCTC	4 cut(s)	1252, 1826, 1886, 2150
BstMBI	GATC	9 cut(s)	391, 457, 805, 1395, 1432, 1508, 1974, 2290, 2294
BstMWI	GCNNNNNNNGC	1 cut(s)	75
BstNI	CCWGG	2 cut(s)	30, 45
BstNSI	RCATGY	2 cut(s)	456, 2003
BstPI	GGTNACC	1 cut(s)	838
BstSCI	CCNGG	2 cut(s)	28, 43
BstSFI	CTRYAG	5 cut(s)	151, 520, 1363, 1581, 1969
BstSLI	GKGCMC	1 cut(s)	44
BstUI	CGCG	1 cut(s)	1661
BstV1I	GCAGC	2 cut(s)	1231, 1364
BstV2I	GAAGAC	7 cut(s)	323, 476, 527, 617, 725, 1566, 1819
BstX2I	RGATCY	3 cut(s)	1395, 1508, 1974
BstYI	RGATCY	3 cut(s)	1395, 1508, 1974
Bsu15I	ATCGAT	2 cut(s)	1626, 2293
Bsu36I	CCTNAGG	1 cut(s)	175
BsuI	GTATCC	2 cut(s)	28, 152
BsuRI	GGCC	2 cut(s)	181, 311
BsuTUI	ATCGAT	2 cut(s)	1626, 2293
BtsCI	GGATG	5 cut(s)	69, 274, 1677, 1834, 2050
BtsIMutI	CAGTG	1 cut(s)	2205
CaiI	CAGNNNCTG	1 cut(s)	346
CciI	TCATGA	2 cut(s)	124, 1555
CfoI	GCGC	2 cut(s)	50, 2119
Cfr13I	GGNCC	5 cut(s)	179, 224, 310, 569, 1672
ClaI	ATCGAT	2 cut(s)	1626, 2293
CseI	GACGC	1 cut(s)	1650
Csp6I	GTAC	6 cut(s)	131, 292, 1147, 1495, 1931, 2234
CviQI	GTAC	6 cut(s)	131, 292, 1147, 1495, 1931, 2234
DdeI	CTNAG	7 cut(s)	175, 230, 251, 1712, 2050, 2135, 2147
DinI	GGCGCC	1 cut(s)	2118
DpnI	GATC	9 cut(s)	393, 459, 807, 1397, 1434, 1510, 1976, 2292, 2296
DpnII	GATC	9 cut(s)	391, 457, 805, 1395, 1432, 1508, 1974, 2290, 2294
DraI	TTTAAA	2 cut(s)	1038, 2302
DriI	GACNNNNNGTC	1 cut(s)	1839
Eam1104I	CTCTTC	3 cut(s)	1246, 1288, 1640
Eam1105I	GACNNNNNGTC	1 cut(s)	1839
EarI	CTCTTC	3 cut(s)	1246, 1288, 1640
Eco130I	CCWWGG	3 cut(s)	82, 1836, 1926
Eco31I	GGTCTC	1 cut(s)	1826
Eco47I	GGWCC	3 cut(s)	224, 569, 1672
Eco57I	CTGAAG	3 cut(s)	335, 503, 2207
Eco81I	CCTNAGG	1 cut(s)	175
Eco91I	GGTNACC	1 cut(s)	838
EcoO109I	RGGNCCY	3 cut(s)	179, 310, 1672
EcoO65I	GGTNACC	1 cut(s)	838
EcoRII	CCWGG	2 cut(s)	28, 43
EcoT14I	CCWWGG	3 cut(s)	82, 1836, 1926
EgeI	GGCGCC	1 cut(s)	2118
EheI	GGCGCC	1 cut(s)	2118
ErhI	CCWWGG	3 cut(s)	82, 1836, 1926
Esp3I	CGTCTC	1 cut(s)	1252
FalI	AAGNNNNNCTT	2 cut(s)	2033, 2065
FaqI	GGGAC	1 cut(s)	1915
FbaI	TGATCA	1 cut(s)	805
Fnu4HI	GCNGC	2 cut(s)	1220, 1378
FokI	GGATG	5 cut(s)	56, 261, 1664, 1841, 2057
Fsp4HI	GCNGC	2 cut(s)	1220, 1378
FspBI	CTAG	3 cut(s)	461, 645, 1202
GlaI	GCGC	2 cut(s)	49, 2118
GluI	GCNGC	2 cut(s)	1220, 1378
GsaI	CCCAGC	1 cut(s)	1650
HaeII	RGCGCY	1 cut(s)	2120
HaeIII	GGCC	2 cut(s)	181, 311
HapII	CCGG	2 cut(s)	504, 2182
HgaI	GACGC	1 cut(s)	1650
HhaI	GCGC	2 cut(s)	50, 2119
Hin1I	GRCGYC	1 cut(s)	2117
Hin6I	GCGC	2 cut(s)	48, 2117
HinP1I	GCGC	2 cut(s)	48, 2117
HpaII	CCGG	2 cut(s)	504, 2182
Hpy166II	GTNNAC	1 cut(s)	1139
Hpy188I	TCNGA	5 cut(s)	348, 1437, 1504, 1666, 2290
Hpy188III	TCNNGA	9 cut(s)	125, 340, 803, 809, 1343, 1399, 1487, 1556, 2137
Hpy8I	GTNNAC	1 cut(s)	1139
HpyCH4III	ACNGT	1 cut(s)	1414
HpyCH4IV	ACGT	2 cut(s)	281, 2079
HpyCH4V	TGCA	9 cut(s)	78, 90, 331, 493, 1272, 1365, 1705, 2003, 2083
HpyF10VI	GCNNNNNNNGC	1 cut(s)	75
HpyF3I	CTNAG	7 cut(s)	175, 230, 251, 1712, 2050, 2135, 2147
HpySE526I	ACGT	2 cut(s)	281, 2079
Hsp92I	GRCGYC	1 cut(s)	2117
HspAI	GCGC	2 cut(s)	48, 2117
KasI	GGCGCC	1 cut(s)	2116
Ksp22I	TGATCA	1 cut(s)	805
Kzo9I	GATC	9 cut(s)	391, 457, 805, 1395, 1432, 1508, 1974, 2290, 2294
LmnI	GCTCC	6 cut(s)	1294, 1505, 1619, 1655, 2199, 2218
Lsp1109I	GCAGC	2 cut(s)	1231, 1364
LweI	GCATC	2 cut(s)	78, 2035
MaeI	CTAG	3 cut(s)	461, 645, 1202
MaeII	ACGT	2 cut(s)	281, 2079
MaeIII	GTNAC	4 cut(s)	838, 1532, 1862, 1883
MalI	GATC	9 cut(s)	393, 459, 807, 1397, 1434, 1510, 1976, 2292, 2296
MbiI	CCGCTC	1 cut(s)	1614
MboI	GATC	9 cut(s)	391, 457, 805, 1395, 1432, 1508, 1974, 2290, 2294
MfeI	CAATTG	1 cut(s)	262
MflI	RGATCY	3 cut(s)	1395, 1508, 1974
MhlI	GDGCHC	1 cut(s)	44
Mly113I	GGCGCC	1 cut(s)	2117
MlyI	GAGTC	5 cut(s)	292, 313, 337, 1693, 1897
MmeI	TCCRAC	5 cut(s)	302, 326, 347, 1234, 1677
MroXI	GAANNNNTTC	2 cut(s)	624, 1354
MslI	CAYNNNNRTG	1 cut(s)	2088
MspA1I	CMGCKG	1 cut(s)	229
MspI	CCGG	2 cut(s)	504, 2182
MspR9I	CCNGG	2 cut(s)	30, 45
MunI	CAATTG	1 cut(s)	262
Mva1269I	GAATGC	1 cut(s)	140
MvaI	CCWGG	2 cut(s)	30, 45
MvnI	CGCG	1 cut(s)	1661
MwoI	GCNNNNNNNGC	1 cut(s)	75
NarI	GGCGCC	1 cut(s)	2117
NdeII	GATC	9 cut(s)	391, 457, 805, 1395, 1432, 1508, 1974, 2290, 2294
NlaIV	GGNNCC	5 cut(s)	41, 744, 1976, 2118, 2180
NmeAIII	GCCGAG	1 cut(s)	2134
NmuCI	GTSAC	2 cut(s)	838, 1862
NspI	RCATGY	2 cut(s)	456, 2003
NspV	TTCGAA	3 cut(s)	1391, 1458, 2269
PagI	TCATGA	2 cut(s)	124, 1555
PctI	GAATGC	1 cut(s)	140
PdmI	GAANNNNTTC	2 cut(s)	624, 1354
PfeI	GAWTC	5 cut(s)	507, 1188, 1573, 1628, 2093
PflMI	CCANNNNNTGG	1 cut(s)	835
PkrI	GCNGC	2 cut(s)	1221, 1379
PleI	GAGTC	5 cut(s)	292, 313, 337, 1693, 1897
PluTI	GGCGCC	1 cut(s)	2120
PpsI	GAGTC	5 cut(s)	292, 313, 337, 1693, 1897
Ppu21I	YACGTR	1 cut(s)	2080
PpuMI	RGGWCCY	1 cut(s)	1672
PsiI	TTATAA	1 cut(s)	663
Psp1406I	AACGTT	1 cut(s)	281
Psp5II	RGGWCCY	1 cut(s)	1672
Psp6I	CCWGG	2 cut(s)	28, 43
PspEI	GGTNACC	1 cut(s)	838
PspFI	CCCAGC	1 cut(s)	1646
PspGI	CCWGG	2 cut(s)	28, 43
PspN4I	GGNNCC	5 cut(s)	41, 744, 1976, 2118, 2180
PspPI	GGNCC	5 cut(s)	179, 224, 310, 569, 1672
PspPPI	RGGWCCY	1 cut(s)	1672
PstI	CTGCAG	1 cut(s)	1367
PstNI	CAGNNNCTG	1 cut(s)	346
PsuI	RGATCY	3 cut(s)	1395, 1508, 1974
RsaI	GTAC	6 cut(s)	132, 293, 1148, 1496, 1932, 2235
RsaNI	GTAC	6 cut(s)	131, 292, 1147, 1495, 1931, 2234
RseI	CAYNNNNRTG	1 cut(s)	2088
SatI	GCNGC	2 cut(s)	1220, 1378
Sau3AI	GATC	9 cut(s)	391, 457, 805, 1395, 1432, 1508, 1974, 2290, 2294
Sau96I	GGNCC	5 cut(s)	179, 224, 310, 569, 1672
SchI	GAGTC	5 cut(s)	292, 313, 337, 1693, 1897
ScrFI	CCNGG	2 cut(s)	30, 45
SduI	GDGCHC	1 cut(s)	44
SfaNI	GCATC	2 cut(s)	78, 2035
SfcI	CTRYAG	5 cut(s)	151, 520, 1363, 1581, 1969
SfoI	GGCGCC	1 cut(s)	2118
SfuI	TTCGAA	3 cut(s)	1391, 1458, 2269
SinI	GGWCC	3 cut(s)	224, 569, 1672
SmiI	ATTTAAAT	1 cut(s)	1038
SmiMI	CAYNNNNRTG	1 cut(s)	2088
SmlI	CTYRAG	2 cut(s)	183, 1322
SmoI	CTYRAG	2 cut(s)	183, 1322
SsiI	CCGC	4 cut(s)	227, 464, 1612, 1659
SspDI	GGCGCC	1 cut(s)	2116
SspI	AATATT	2 cut(s)	245, 1406
SspMI	CTAG	3 cut(s)	461, 645, 1202
StyD4I	CCNGG	2 cut(s)	28, 43
StyI	CCWWGG	3 cut(s)	82, 1836, 1926
SwaI	ATTTAAAT	1 cut(s)	1038
TaaI	ACNGT	1 cut(s)	1414
TaiI	ACGT	2 cut(s)	284, 2082
TaqI	TCGA	9 cut(s)	394, 1391, 1431, 1458, 1626, 1638, 1935, 2269, 2293
TatI	WGTACW	3 cut(s)	291, 1146, 1494
TfiI	GAWTC	5 cut(s)	507, 1188, 1573, 1628, 2093
TscAI	CASTG	1 cut(s)	2212
TseFI	GTSAC	2 cut(s)	838, 1862
TseI	GCWGC	2 cut(s)	1219, 1377
Tsp45I	GTSAC	2 cut(s)	838, 1862
TspGWI	ACGGA	2 cut(s)	62, 1234
TspRI	CASTG	1 cut(s)	2212
Van91I	CCANNNNNTGG	1 cut(s)	835
VpaK11BI	GGWCC	3 cut(s)	224, 569, 1672
XceI	RCATGY	2 cut(s)	456, 2003
XcmI	CCANNNNNNNNNTGG	1 cut(s)	484
XmnI	GAANNNNTTC	2 cut(s)	624, 1354
XspI	CTAG	3 cut(s)	461, 645, 1202

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.