Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

SuperScaffold_520

Orthogroup ID

N/A

Physical Location & Seq

Genomic Coordinates Forward (+)

292529 .. 295946

Gene Structure 3418 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1653 bp

ATGGAAGGGATAGACCTTTTGTTTTATCAAGAGCATTATTTGCTGGAAGTCAAAGGCATGGAGCAGTATGGACAGGAGATAATTCAGCTGATTGGGATCGACCTTAGGGTTTCAGTTCCAATGGTTTTGACACTTGGTCTTACTGGGATCTCATTCTCAGGTGCTGATGTTGGTGGGTTTTTTGGCAATCCAGAACCTGAGTTGTTAGTTCGTTGGTATCAGTTAGGGGCATACTATCCATTTTTTAGAGGCCATGCCCATCATGACACCAAAAGGCGAGAACCATGGTTGTTTGGGCTCAAAGTCGCAATTATAGGGAATTCAAAAAGAAATAAAGTAGGCAATATTATAGGAAATTTAAAGGAAAATAAAGGCTTAGTGAGTGGATCAGTTATAGGGAAACTCAAGAGAAATAAATCTTTGTTAATCAAAATGCTAGGTGGCGGAATCTACATGGAGAAACTATCTTCTTCGTTGAAACTAGAACGAGAAAAAAATACTGAACGTATTAGGGAGGCCATACGCATTCGTTACATGTTGCTTCCATATTTCTACACATTATTCAGAGAGGCAAGCACAACTGGTGTGCCAGTTGTTCGTCCATTATGGATGGAGTTTCCTTCTGAAGAAAATACTTTCAGCAATGACGAGGCTTTTATGATTGGAAACAGTATTCTGGTGCAAGGGATTTATGCAGAGCGTGCAAGACATGCTTCAGTGTTTTTTCCCGGAAAGGAATTATGGTATGACACAAAAACTGGAGTCGCATACAAGGGAGGTAAAATCTATAAGTTGGAAGTTAATGAAGAAAGTGTTCCTGCCTTCCAAAGGGCTGGAACCATCATACCAAGGAAAGACAGGTTTCGTCGAAGTTCCACACAGATGGTGAATGATCCATACACTCTGGACTTCTCCATTCGAGGGCTCACATTGATAGTCTATTTCTTATCCTTGCTTGAATCCCAGTCTCTCTTTGGTAAATATCAAAGAAAATCCTCATTGAACTTCATCATGTTGAGGACATTGTTCCTACCCATAATAAATACTTGTTGGCAAAGAACAAAGGTCTCTACCCATACGTCTGAGACGACCAATTTAGCGAGAAAATCTGGAGGGGTGTGGATTTTAAGATTGGGGTATACTTCTGTACGGATCTGGAGATTAAAGATGCACTTGACATTTACCGTAATAAGAAATACCGGTAGGTTTCATAATTCATTAGTGATAGCACTTAATAGTTCACAAGCAGCTGAAGGTGAACTCTATGTCGATGATGGTAGGAGCTTTGAGTTTCAGAAAGGGGCCTACATCCACCGTCGTTTCATATTCTCAGAAGGGAAGCTTACTTCTCTTAATCTGGCATCCGCTGCCTCTGGCCAAAACGAATTTTCTTGTGGATGTGTCATTGAGAGGATTATTCTGCAAGGACTCTCCACTGGGCACAAAAGCGCTCTAATTGAACCCGCAAGTCAGAAGGCCGAAATTGAACTGGGCCCACTTCTCATGCATTCGAGGCAAGGTCCAACTGCGATAACAATCCGCAAACCCAATGTCCGGATAGCCGATGATTGGGTGATAAAAATGTTAAAAGTCTCCTGTAGGACTTTTTTTTATTCTTTTCTTGGGTTTAAGGAGGGATATTTCTTAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000902 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006491 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009250 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009826 GO:0009987 GO:0010035 GO:0010038 GO:0012505 GO:0015926 GO:0016043 GO:0016049 GO:0016051 GO:0016787 GO:0016798 GO:0019538 GO:0030243 GO:0030244 GO:0030587 GO:0031288 GO:0032502 GO:0032989 GO:0033692 GO:0033919 GO:0034637 GO:0034645 GO:0036211 GO:0040007 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043687 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048589 GO:0048856 GO:0048869 GO:0050896 GO:0051273 GO:0051274 GO:0051703 GO:0051704 GO:0051707 GO:0060560 GO:0071704 GO:0071840 GO:0090599 GO:0090600 GO:0090702 GO:0098542 GO:0099120 GO:1901135 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00510 ko01100 ko04141 map00510 map01100 map04141

Pfam Domains

Protein Families

DUF5110 Gal_mutarotas_2 Glyco_hydro_31

Protein Analysis

551

Amino Acids

62.59

Weight (kDa)

9.67

Isoelectric Point (pI)

42.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_31_2nd	PF01055	34 - 103	3.9e-25	Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd	PF21365	195 - 282	1.1e-22	Glycosyl hydrolase family 31 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1139
AccIII	TCCGGA	1 cut(s)	1554
AciI	CCGC	4 cut(s)	444, 1365, 1464, 1540
AclWI	GGATC	5 cut(s)	104, 155, 394, 887, 1160
AcoI	YGGCCR	1 cut(s)	1375
AcsI	RAATTY	3 cut(s)	319, 355, 1385
AcuI	CTGAAG	3 cut(s)	645, 699, 1272
AfaI	GTAC	1 cut(s)	1149
AfeI	AGCGCT	1 cut(s)	1450
AfiI	CCNNNNNNNGG	4 cut(s)	828, 921, 1554, 1569
AflIII	ACRYGT	1 cut(s)	534
AgeI	ACCGGT	1 cut(s)	1199
AgsI	TTSAA	6 cut(s)	324, 478, 959, 1003, 1460, 1487
AhdI	GACNNNNNGTC	1 cut(s)	135
AluBI	AGCT	4 cut(s)	88, 1250, 1284, 1342
AluI	AGCT	4 cut(s)	88, 1250, 1284, 1342
Alw26I	GTCTC	4 cut(s)	972, 1072, 1079, 1597
AlwI	GGATC	5 cut(s)	104, 155, 394, 887, 1160
AlwNI	CAGNNNCTG	2 cut(s)	164, 197
Aor13HI	TCCGGA	1 cut(s)	1554
Aor51HI	AGCGCT	1 cut(s)	1450
AoxI	GGCC	6 cut(s)	250, 516, 1302, 1375, 1476, 1492
ApaI	GGGCCC	1 cut(s)	1496
ApeKI	GCWGC	2 cut(s)	1247, 1367
ApoI	RAATTY	3 cut(s)	319, 355, 1385
ArsI	GACNNNNNNTTYG	2 cut(s)	1536, 1568
AsiGI	ACCGGT	1 cut(s)	1199
Asp700I	GAANNNNTTC	1 cut(s)	813
AspLEI	GCGC	1 cut(s)	1451
AspS9I	GGNCC	4 cut(s)	1302, 1492, 1493, 1520
AsuC2I	CCSGG	1 cut(s)	729
AsuHPI	GGTGA	3 cut(s)	898, 1268, 1585
AvaII	GGWCC	1 cut(s)	1520
AxyI	CCTNAGG	1 cut(s)	104
BaeGI	GKGCMC	2 cut(s)	1443, 1496
BalI	TGGCCA	1 cut(s)	1377
BanII	GRGCYC	3 cut(s)	300, 927, 1496
BbvI	GCAGC	2 cut(s)	1259, 1354
BccI	CCATC	5 cut(s)	267, 604, 848, 877, 1268
BcnI	CCSGG	1 cut(s)	729
BcoDI	GTCTC	4 cut(s)	972, 1072, 1079, 1597
BfaI	CTAG	2 cut(s)	437, 482
BfmI	CTRYAG	1 cut(s)	1597
BfoI	RGCGCY	1 cut(s)	1452
BisI	GCNGC	2 cut(s)	1248, 1368
BlsI	GCNGC	2 cut(s)	1249, 1369
Bme1390I	CCNGG	1 cut(s)	729
Bme18I	GGWCC	1 cut(s)	1520
BmeRI	GACNNNNNGTC	1 cut(s)	135
BmgT120I	GGNCC	4 cut(s)	1302, 1492, 1493, 1520
BmiI	GGNNCC	3 cut(s)	838, 1303, 1494
BmrFI	CCNGG	1 cut(s)	729
BmrI	ACTGGG	4 cut(s)	153, 958, 1446, 1499
BmsI	GCATC	2 cut(s)	1158, 1370
BmuI	ACTGGG	4 cut(s)	153, 958, 1446, 1499
BpmI	CTGGAG	3 cut(s)	780, 1131, 1177
BpuEI	CTTGAG	1 cut(s)	389
BpuMI	CCSGG	1 cut(s)	729
BsaBI	GATNNNNATC	2 cut(s)	95, 1535
BsaI	GGTCTC	1 cut(s)	1072
BsaJI	CCNNGG	2 cut(s)	284, 848
BsaWI	WCCGGW	2 cut(s)	1199, 1554
Bsc4I	CCNNNNNNNGG	4 cut(s)	828, 921, 1554, 1569
Bse118I	RCCGGY	1 cut(s)	1199
Bse1I	ACTGG	7 cut(s)	148, 586, 590, 763, 964, 1441, 1494
Bse21I	CCTNAGG	1 cut(s)	104
Bse3DI	GCAATG	1 cut(s)	649
Bse8I	GATNNNNATC	2 cut(s)	95, 1535
BseAI	TCCGGA	1 cut(s)	1554
BseDI	CCNNGG	2 cut(s)	284, 848
BseGI	GGATG	4 cut(s)	615, 1308, 1361, 1403
BseJI	GATNNNNATC	2 cut(s)	95, 1535
BseLI	CCNNNNNNNGG	4 cut(s)	828, 921, 1554, 1569
BseMI	GCAATG	1 cut(s)	649
BseMII	CTCAG	4 cut(s)	171, 189, 1074, 1344
BseNI	ACTGG	7 cut(s)	148, 586, 590, 763, 964, 1441, 1494
BseSI	GKGCMC	2 cut(s)	1443, 1496
BseXI	GCAGC	2 cut(s)	1259, 1354
BshFI	GGCC	6 cut(s)	252, 518, 1304, 1377, 1478, 1494
BshTI	ACCGGT	1 cut(s)	1199
BsiSI	CCGG	3 cut(s)	729, 1200, 1555
BslI	CCNNNNNNNGG	4 cut(s)	828, 921, 1554, 1569
BsmAI	GTCTC	4 cut(s)	972, 1072, 1079, 1597
BsmBI	CGTCTC	1 cut(s)	1079
BsmI	GAATGC	2 cut(s)	525, 1507
BsnI	GGCC	6 cut(s)	252, 518, 1304, 1377, 1478, 1494
Bso31I	GGTCTC	1 cut(s)	1072
Bsp120I	GGGCCC	1 cut(s)	1492
Bsp1286I	GDGCHC	4 cut(s)	300, 927, 1443, 1496
Bsp13I	TCCGGA	1 cut(s)	1554
Bsp143I	GATC	5 cut(s)	96, 147, 386, 892, 1152
Bsp19I	CCATGG	1 cut(s)	284
BspACI	CCGC	4 cut(s)	444, 1365, 1464, 1540
BspANI	GGCC	6 cut(s)	252, 518, 1304, 1377, 1478, 1494
BspCNI	CTCAG	4 cut(s)	170, 190, 1075, 1343
BspEI	TCCGGA	1 cut(s)	1554
BspHI	TCATGA	1 cut(s)	262
BspLI	GGNNCC	3 cut(s)	838, 1303, 1494
BspPI	GGATC	5 cut(s)	104, 155, 394, 887, 1160
BspTNI	GGTCTC	1 cut(s)	1072
BsrDI	GCAATG	1 cut(s)	649
BsrFI	RCCGGY	1 cut(s)	1199
BsrI	ACTGG	7 cut(s)	148, 586, 590, 763, 964, 1441, 1494
BssAI	RCCGGY	1 cut(s)	1199
BssECI	CCNNGG	2 cut(s)	284, 848
BssMI	GATC	5 cut(s)	96, 147, 386, 892, 1152
BssNAI	GTATAC	1 cut(s)	1140
BssT1I	CCWWGG	2 cut(s)	284, 848
Bst1107I	GTATAC	1 cut(s)	1140
Bst4CI	ACNGT	3 cut(s)	671, 1186, 1316
BstAPI	GCANNNNNTGC	4 cut(s)	40, 701, 710, 1367
BstC8I	GCNNGC	2 cut(s)	574, 702
BstDEI	CTNAG	7 cut(s)	104, 157, 198, 376, 1083, 1330, 1645
BstDSI	CCRYGG	1 cut(s)	284
BstENI	CCTNNNNNAGG	1 cut(s)	826
BstF5I	GGATG	4 cut(s)	615, 1308, 1361, 1403
BstH2I	RGCGCY	1 cut(s)	1452
BstHHI	GCGC	1 cut(s)	1451
BstKTI	GATC	5 cut(s)	99, 150, 389, 895, 1155
BstMAI	GTCTC	4 cut(s)	972, 1072, 1079, 1597
BstMBI	GATC	5 cut(s)	96, 147, 386, 892, 1152
BstMWI	GCNNNNNNNGC	5 cut(s)	40, 701, 710, 1367, 1513
BstNSI	RCATGY	2 cut(s)	538, 713
BstSCI	CCNGG	1 cut(s)	727
BstSFI	CTRYAG	1 cut(s)	1597
BstSLI	GKGCMC	2 cut(s)	1443, 1496
BstV1I	GCAGC	2 cut(s)	1259, 1354
BstX2I	RGATCY	2 cut(s)	147, 1152
BstXI	CCANNNNNNTGG	2 cut(s)	833, 883
BstYI	RGATCY	2 cut(s)	147, 1152
BstZ17I	GTATAC	1 cut(s)	1140
Bsu36I	CCTNAGG	1 cut(s)	104
BsuRI	GGCC	6 cut(s)	252, 518, 1304, 1377, 1478, 1494
BtgI	CCRYGG	1 cut(s)	284
BtsCI	GGATG	4 cut(s)	615, 1308, 1361, 1403
BtsIMutI	CAGTG	2 cut(s)	723, 1434
Cac8I	GCNNGC	2 cut(s)	574, 702
CaiI	CAGNNNCTG	2 cut(s)	164, 197
CciI	TCATGA	1 cut(s)	262
CfoI	GCGC	1 cut(s)	1451
Cfr10I	RCCGGY	1 cut(s)	1199
Cfr13I	GGNCC	4 cut(s)	1302, 1492, 1493, 1520
Csp6I	GTAC	1 cut(s)	1148
CspAI	ACCGGT	1 cut(s)	1199
CviAII	CATG	9 cut(s)	58, 254, 263, 285, 454, 535, 710, 1012, 1504
CviQI	GTAC	1 cut(s)	1148
DdeI	CTNAG	7 cut(s)	104, 157, 198, 376, 1083, 1330, 1645
DpnI	GATC	5 cut(s)	98, 149, 388, 894, 1154
DpnII	GATC	5 cut(s)	96, 147, 386, 892, 1152
DraI	TTTAAA	1 cut(s)	360
DriI	GACNNNNNGTC	1 cut(s)	135
EaeI	YGGCCR	1 cut(s)	1375
Eam1105I	GACNNNNNGTC	1 cut(s)	135
EciI	GGCGGA	1 cut(s)	459
Eco130I	CCWWGG	2 cut(s)	284, 848
Eco24I	GRGCYC	3 cut(s)	300, 927, 1496
Eco31I	GGTCTC	1 cut(s)	1072
Eco47I	GGWCC	1 cut(s)	1520
Eco47III	AGCGCT	1 cut(s)	1450
Eco57I	CTGAAG	3 cut(s)	645, 699, 1272
Eco81I	CCTNAGG	1 cut(s)	104
EcoNI	CCTNNNNNAGG	1 cut(s)	826
EcoO109I	RGGNCCY	1 cut(s)	1302
EcoRI	GAATTC	1 cut(s)	319
EcoT14I	CCWWGG	2 cut(s)	284, 848
EcoT22I	ATGCAT	1 cut(s)	1509
EcoT38I	GRGCYC	3 cut(s)	300, 927, 1496
ErhI	CCWWGG	2 cut(s)	284, 848
Esp3I	CGTCTC	1 cut(s)	1079
FaeI	CATG	9 cut(s)	61, 257, 266, 288, 457, 538, 713, 1015, 1507
FalI	AAGNNNNNCTT	6 cut(s)	697, 729, 1157, 1189, 1326, 1358
FatI	CATG	9 cut(s)	57, 253, 262, 284, 453, 534, 709, 1011, 1503
FauI	CCCGC	1 cut(s)	1471
FblI	GTMKAC	1 cut(s)	1139
Fnu4HI	GCNGC	2 cut(s)	1248, 1368
FokI	GGATG	4 cut(s)	622, 1295, 1348, 1410
FriOI	GRGCYC	3 cut(s)	300, 927, 1496
Fsp4HI	GCNGC	2 cut(s)	1248, 1368
FspBI	CTAG	2 cut(s)	437, 482
GlaI	GCGC	1 cut(s)	1450
GluI	GCNGC	2 cut(s)	1248, 1368
GsuI	CTGGAG	3 cut(s)	780, 1131, 1177
HaeII	RGCGCY	1 cut(s)	1452
HaeIII	GGCC	6 cut(s)	252, 518, 1304, 1377, 1478, 1494
HapII	CCGG	3 cut(s)	729, 1200, 1555
HhaI	GCGC	1 cut(s)	1451
Hin1II	CATG	9 cut(s)	61, 257, 266, 288, 457, 538, 713, 1015, 1507
Hin6I	GCGC	1 cut(s)	1449
HinP1I	GCGC	1 cut(s)	1449
HindIII	AAGCTT	1 cut(s)	1340
HinfI	GANTC	4 cut(s)	447, 762, 959, 1428
HpaII	CCGG	3 cut(s)	729, 1200, 1555
HphI	GGTGA	3 cut(s)	898, 1268, 1585
Hpy166II	GTNNAC	3 cut(s)	1140, 1241, 1259
Hpy188I	TCNGA	6 cut(s)	566, 625, 1084, 1296, 1333, 1473
Hpy188III	TCNNGA	8 cut(s)	29, 191, 263, 406, 905, 1110, 1156, 1555
Hpy8I	GTNNAC	3 cut(s)	1140, 1241, 1259
Hpy99I	CGWCG	2 cut(s)	870, 1320
HpyAV	CCTTC	5 cut(s)	630, 832, 1247, 1328, 1468
HpyCH4III	ACNGT	3 cut(s)	671, 1186, 1316
HpyCH4IV	ACGT	2 cut(s)	505, 1079
HpyCH4V	TGCA	6 cut(s)	682, 695, 704, 1171, 1423, 1507
HpyF10VI	GCNNNNNNNGC	5 cut(s)	40, 701, 710, 1367, 1513
HpyF3I	CTNAG	7 cut(s)	104, 157, 198, 376, 1083, 1330, 1645
HpySE526I	ACGT	2 cut(s)	505, 1079
Hsp92II	CATG	9 cut(s)	61, 257, 266, 288, 457, 538, 713, 1015, 1507
HspAI	GCGC	1 cut(s)	1449
Kpn2I	TCCGGA	1 cut(s)	1554
Kzo9I	GATC	5 cut(s)	96, 147, 386, 892, 1152
LmnI	GCTCC	2 cut(s)	61, 1281
Lsp1109I	GCAGC	2 cut(s)	1259, 1354
LweI	GCATC	2 cut(s)	1158, 1370
MaeI	CTAG	2 cut(s)	437, 482
MaeII	ACGT	2 cut(s)	505, 1079
MaeIII	GTNAC	1 cut(s)	530
MalI	GATC	5 cut(s)	98, 149, 388, 894, 1154
MboI	GATC	5 cut(s)	96, 147, 386, 892, 1152
MboII	GAAGA	4 cut(s)	459, 462, 638, 818
MflI	RGATCY	2 cut(s)	147, 1152
MhlI	GDGCHC	4 cut(s)	300, 927, 1443, 1496
MlsI	TGGCCA	1 cut(s)	1377
MluNI	TGGCCA	1 cut(s)	1377
MlyI	GAGTC	2 cut(s)	771, 1422
MmeI	TCCRAC	2 cut(s)	774, 1547
Mox20I	TGGCCA	1 cut(s)	1377
Mph1103I	ATGCAT	1 cut(s)	1509
MroI	TCCGGA	1 cut(s)	1554
MroXI	GAANNNNTTC	1 cut(s)	813
MscI	TGGCCA	1 cut(s)	1377
MseI	TTAA	9 cut(s)	359, 425, 801, 1127, 1163, 1233, 1353, 1586, 1629
MslI	CAYNNNNRTG	1 cut(s)	881
Msp20I	TGGCCA	1 cut(s)	1377
MspA1I	CMGCKG	3 cut(s)	88, 1250, 1367
MspI	CCGG	3 cut(s)	729, 1200, 1555
MspR9I	CCNGG	1 cut(s)	729
Mva1269I	GAATGC	2 cut(s)	525, 1507
MwoI	GCNNNNNNNGC	5 cut(s)	40, 701, 710, 1367, 1513
NciI	CCSGG	1 cut(s)	729
NcoI	CCATGG	1 cut(s)	284
NdeII	GATC	5 cut(s)	96, 147, 386, 892, 1152
NlaIII	CATG	9 cut(s)	61, 257, 266, 288, 457, 538, 713, 1015, 1507
NlaIV	GGNNCC	3 cut(s)	838, 1303, 1494
NsiI	ATGCAT	1 cut(s)	1509
NspI	RCATGY	2 cut(s)	538, 713
PagI	TCATGA	1 cut(s)	262
PciI	ACATGT	1 cut(s)	534
PcsI	WCGNNNNNNNCGW	1 cut(s)	1085
PctI	GAATGC	2 cut(s)	525, 1507
PdmI	GAANNNNTTC	1 cut(s)	813
PfeI	GAWTC	2 cut(s)	447, 959
PfoI	TCCNGGA	1 cut(s)	727
PinAI	ACCGGT	1 cut(s)	1199
PkrI	GCNGC	2 cut(s)	1249, 1369
PleI	GAGTC	2 cut(s)	770, 1422
PpsI	GAGTC	2 cut(s)	770, 1422
PscI	ACATGT	1 cut(s)	534
PspN4I	GGNNCC	3 cut(s)	838, 1303, 1494
PspOMI	GGGCCC	1 cut(s)	1492
PspPI	GGNCC	4 cut(s)	1302, 1492, 1493, 1520
PstNI	CAGNNNCTG	2 cut(s)	164, 197
PsuI	RGATCY	2 cut(s)	147, 1152
PvuII	CAGCTG	2 cut(s)	88, 1250
RsaI	GTAC	1 cut(s)	1149
RsaNI	GTAC	1 cut(s)	1148
RseI	CAYNNNNRTG	1 cut(s)	881
SaqAI	TTAA	9 cut(s)	359, 425, 801, 1127, 1163, 1233, 1353, 1586, 1629
SatI	GCNGC	2 cut(s)	1248, 1368
Sau3AI	GATC	5 cut(s)	96, 147, 386, 892, 1152
Sau96I	GGNCC	4 cut(s)	1302, 1492, 1493, 1520
SchI	GAGTC	2 cut(s)	771, 1422
ScrFI	CCNGG	1 cut(s)	729
SduI	GDGCHC	4 cut(s)	300, 927, 1443, 1496
SfaNI	GCATC	2 cut(s)	1158, 1370
SfcI	CTRYAG	1 cut(s)	1597
SinI	GGWCC	1 cut(s)	1520
SmiMI	CAYNNNNRTG	1 cut(s)	881
SmlI	CTYRAG	1 cut(s)	404
SmoI	CTYRAG	1 cut(s)	404
SsiI	CCGC	4 cut(s)	444, 1365, 1464, 1540
SspI	AATATT	1 cut(s)	346
SspMI	CTAG	2 cut(s)	437, 482
StyD4I	CCNGG	1 cut(s)	727
StyI	CCWWGG	2 cut(s)	284, 848
TaaI	ACNGT	3 cut(s)	671, 1186, 1316
TaiI	ACGT	2 cut(s)	508, 1082
TaqI	TCGA	5 cut(s)	99, 868, 919, 1269, 1511
TfiI	GAWTC	2 cut(s)	447, 959
Tru1I	TTAA	9 cut(s)	359, 425, 801, 1127, 1163, 1233, 1353, 1586, 1629
Tru9I	TTAA	9 cut(s)	359, 425, 801, 1127, 1163, 1233, 1353, 1586, 1629
TscAI	CASTG	2 cut(s)	723, 1441
TseI	GCWGC	2 cut(s)	1247, 1367
TspDTI	ATGAA	5 cut(s)	819, 997, 1199, 1206, 1312
TspGWI	ACGGA	1 cut(s)	1165
TspRI	CASTG	2 cut(s)	723, 1441
VpaK11BI	GGWCC	1 cut(s)	1520
XagI	CCTNNNNNAGG	1 cut(s)	826
XapI	RAATTY	3 cut(s)	319, 355, 1385
XceI	RCATGY	2 cut(s)	538, 713
XcmI	CCANNNNNNNNNTGG	1 cut(s)	971
XmiI	GTMKAC	1 cut(s)	1139
XmnI	GAANNNNTTC	1 cut(s)	813
XspI	CTAG	2 cut(s)	437, 482
Zsp2I	ATGCAT	1 cut(s)	1509

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.