pycom520g00900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
N/A
Physical Location & Seq
Forward (+)
690434 .. 691036
603 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 363 bp
ATGTCAACATCAATAATTTCTACCATAGTTTTGAAATTATTTTGCTTTTGCTTTTTGCAGGTTCTCAAACAGATTGAAACAGTTATAAAGTTAACATGGGTTAGGAACACACACTATGTGGAAAGGTTGGTGGACGAACTTGCTGTTTTGTACAAAGAAGCAGCATTGAAAAGGTCTCGTGAAGGAATTAGAGATCTTGTTATTCGGATACTGATCCTGCTCCACCAATGCAAAGGCATGCAGTTCGAAGCAGCCTGGGGAAAGCACAGAGATGATCCAAACTTAGCGACCATTGGTGCCAGTTTAGGTGAACGAAAAGCAACGGTAAATTTACCATCATGCAGCATATTTGCAATAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

13.66

Weight (kDa)

9.23

Isoelectric Point (pI)

28.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 86
Acc36I ACCTGC 1 cut(s) 49
AccB1I GGYRCC 1 cut(s) 296
AclWI GGATC 2 cut(s) 208, 269
AcsI RAATTY 1 cut(s) 328
AdeI CACNNNGTG 1 cut(s) 118
AfaI GTAC 1 cut(s) 152
AgsI TTSAA 3 cut(s) 34, 77, 169
AjnI CCWGG 1 cut(s) 254
Alw26I GTCTC 1 cut(s) 180
AlwI GGATC 2 cut(s) 208, 269
ApeKI GCWGC 3 cut(s) 161, 251, 342
ApoI RAATTY 1 cut(s) 328
AsuHPI GGTGA 1 cut(s) 320
AsuII TTCGAA 1 cut(s) 246
BanI GGYRCC 1 cut(s) 296
BauI CACGAG 1 cut(s) 177
BbvI GCAGC 3 cut(s) 173, 263, 354
BccI CCATC 1 cut(s) 343
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BciT130I CCWGG 1 cut(s) 256
BciVI GTATCC 1 cut(s) 201
BcoDI GTCTC 1 cut(s) 180
BfuAI ACCTGC 1 cut(s) 49
BfuI GTATCC 1 cut(s) 201
BglII AGATCT 1 cut(s) 193
BisI GCNGC 3 cut(s) 162, 252, 343
BlsI GCNGC 3 cut(s) 163, 253, 344
Bme1390I CCNGG 1 cut(s) 256
BmiI GGNNCC 1 cut(s) 298
BmrFI CCNGG 1 cut(s) 256
Bpu14I TTCGAA 1 cut(s) 246
BsaBI GATNNNNATC 1 cut(s) 212
BsaI GGTCTC 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 255
Bse1I ACTGG 1 cut(s) 300
Bse8I GATNNNNATC 1 cut(s) 212
BseBI CCWGG 1 cut(s) 256
BseDI CCNNGG 1 cut(s) 255
BseJI GATNNNNATC 1 cut(s) 212
BseNI ACTGG 1 cut(s) 300
BseXI GCAGC 3 cut(s) 173, 263, 354
BshNI GGYRCC 1 cut(s) 296
BsmAI GTCTC 1 cut(s) 180
Bso31I GGTCTC 1 cut(s) 180
Bsp119I TTCGAA 1 cut(s) 246
Bsp1407I TGTACA 1 cut(s) 150
Bsp143I GATC 3 cut(s) 193, 213, 274
BspLI GGNNCC 1 cut(s) 298
BspMI ACCTGC 1 cut(s) 49
BspPI GGATC 2 cut(s) 208, 269
BspT104I TTCGAA 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 296
BspTNI GGTCTC 1 cut(s) 180
BsrGI TGTACA 1 cut(s) 150
BsrI ACTGG 1 cut(s) 300
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 3 cut(s) 193, 213, 274
BssSI CACGAG 1 cut(s) 177
Bst2BI CACGAG 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 256
Bst4CI ACNGT 2 cut(s) 82, 325
BstAUI TGTACA 1 cut(s) 150
BstBI TTCGAA 1 cut(s) 246
BstC8I GCNNGC 1 cut(s) 239
BstDEI CTNAG 1 cut(s) 283
BstKTI GATC 3 cut(s) 196, 216, 277
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 3 cut(s) 193, 213, 274
BstNI CCWGG 1 cut(s) 256
BstNSI RCATGY 1 cut(s) 241
BstSCI CCNGG 1 cut(s) 254
BstV1I GCAGC 3 cut(s) 173, 263, 354
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BsuI GTATCC 1 cut(s) 201
BveI ACCTGC 1 cut(s) 49
Cac8I GCNNGC 1 cut(s) 239
Csp6I GTAC 1 cut(s) 151
CviAII CATG 3 cut(s) 96, 238, 339
CviJI RGCY 1 cut(s) 254
CviKI_1 RGCY 1 cut(s) 254
CviQI GTAC 1 cut(s) 151
DdeI CTNAG 1 cut(s) 283
DpnI GATC 3 cut(s) 195, 215, 276
DpnII GATC 3 cut(s) 193, 213, 274
DraIII CACNNNGTG 1 cut(s) 118
Eco31I GGTCTC 1 cut(s) 180
EcoRII CCWGG 1 cut(s) 254
FaeI CATG 3 cut(s) 99, 241, 342
FaiI YATR 7 cut(s) 26, 86, 97, 117, 239, 340, 347
FatI CATG 3 cut(s) 95, 237, 338
Fnu4HI GCNGC 3 cut(s) 162, 252, 343
Fsp4HI GCNGC 3 cut(s) 162, 252, 343
GluI GCNGC 3 cut(s) 162, 252, 343
Hin1II CATG 3 cut(s) 99, 241, 342
HincII GTYRAC 2 cut(s) 6, 93
HindII GTYRAC 2 cut(s) 6, 93
HpaI GTTAAC 1 cut(s) 93
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 4 cut(s) 6, 93, 133, 311
Hpy188I TCNGA 1 cut(s) 207
Hpy188III TCNNGA 1 cut(s) 179
Hpy8I GTNNAC 4 cut(s) 6, 93, 133, 311
HpyAV CCTTC 1 cut(s) 176
HpyCH4III ACNGT 2 cut(s) 82, 325
HpyCH4V TGCA 5 cut(s) 58, 231, 241, 342, 353
HpyF3I CTNAG 1 cut(s) 283
Hsp92II CATG 3 cut(s) 99, 241, 342
KspAI GTTAAC 1 cut(s) 93
Kzo9I GATC 3 cut(s) 193, 213, 274
LmnI GCTCC 1 cut(s) 225
LpnPI CCDG 5 cut(s) 44, 230, 241, 268, 313
Lsp1109I GCAGC 3 cut(s) 173, 263, 354
MalI GATC 3 cut(s) 195, 215, 276
MboI GATC 3 cut(s) 193, 213, 274
MflI RGATCY 1 cut(s) 193
MluCI AATT 4 cut(s) 15, 35, 186, 328
MseI TTAA 1 cut(s) 92
MslI CAYNNNNRTG 1 cut(s) 270
MspR9I CCNGG 1 cut(s) 256
MvaI CCWGG 1 cut(s) 256
NdeII GATC 3 cut(s) 193, 213, 274
NlaIII CATG 3 cut(s) 99, 241, 342
NlaIV GGNNCC 1 cut(s) 298
NspI RCATGY 1 cut(s) 241
NspV TTCGAA 1 cut(s) 246
PaeI GCATGC 1 cut(s) 241
PkrI GCNGC 3 cut(s) 163, 253, 344
PsiI TTATAA 1 cut(s) 86
Psp6I CCWGG 1 cut(s) 254
PspGI CCWGG 1 cut(s) 254
PspN4I GGNNCC 1 cut(s) 298
PsuI RGATCY 1 cut(s) 193
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
RseI CAYNNNNRTG 1 cut(s) 270
SaqAI TTAA 1 cut(s) 92
SatI GCNGC 3 cut(s) 162, 252, 343
Sau3AI GATC 3 cut(s) 193, 213, 274
ScrFI CCNGG 1 cut(s) 256
SetI ASST 4 cut(s) 63, 128, 176, 310
SfuI TTCGAA 1 cut(s) 246
SmiMI CAYNNNNRTG 1 cut(s) 270
SphI GCATGC 1 cut(s) 241
Sse9I AATT 4 cut(s) 15, 35, 186, 328
StyD4I CCNGG 1 cut(s) 254
TaaI ACNGT 2 cut(s) 82, 325
TaqI TCGA 1 cut(s) 246
TasI AATT 4 cut(s) 15, 35, 186, 328
TatI WGTACW 1 cut(s) 150
Tru1I TTAA 1 cut(s) 92
Tru9I TTAA 1 cut(s) 92
TseI GCWGC 3 cut(s) 161, 251, 342
XapI RAATTY 1 cut(s) 328
XceI RCATGY 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.