RchiOBHm_Chr7g0183721

ATP-dependent helicase hrq1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
N/A
Physical Location & Seq
Reverse (-)
4494890 .. 4495898
1009 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 738 bp
ATGGCAGCGACTCGGATTCAAATCGAGGTCCGTACATTGACCGGCGAAGCGACCACCGTCTCAGCCTCCGTCAACGACGCCGTTTCAGACCTCAAGCTACTTCTCAACCAGTCCTTCCCTCCCGCCACCAACTCTCCCAATTTCCACCTCTTCTTCAAGGGTGAGAAGTTGAGGTTGCAGAGTCGAATAGGCACACACGGGATTAAGCCGGGTGAGGTTCTAGTGCTGGTCCCGTTTGTTAAAAAGGTGGCCAATAATCAGAGCCAGAAATGTGATCAATCTGATCCATCGTTGAATACTTCTGCTAATCGCTCCATGTCCAAGTTTGCGAACTCCGCATGGTCTGATATGATGCAGGACTTGTCATATTTGCGTGATGGTTCGAGTGATGGAACCCCCAGTGTGTCAAATATAGGGAGTTTTAGTGTTGGAGATGGAAGTGGAGATTGCTCTTGTGAAGTGAAGCGGAAGAGGGGGTTTGGTTGTGATGAGATGATATTGGATATGTTGTGGGCTAGTAGAAGCAAGAGTGTTCTTGATGAACAGAATTCTAGAAGGTTTGTTGAGCTTTTGGAGTCGGTGAATTGTTTATCGGATCCATATTCTGGGGACTGCATGTTGTGGAGAAGAAAAGCTAGCTTACAAGGCTTTGGCTTGGGGCTGCCTAACAGCAATGGAGAGGACAACCTCAACTCTATTGGAGCAAGCACTGAAACAGCTTGCGACGTTTGGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DUF1998 Helicase_C

Protein Analysis

245

Amino Acids

26.61

Weight (kDa)

5.49

Isoelectric Point (pI)

53.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 605
AciI CCGC 3 cut(s) 123, 336, 466
AclWI GGATC 3 cut(s) 278, 590, 603
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 1 cut(s) 547
AcyI GRCGYC 1 cut(s) 78
AfaI GTAC 1 cut(s) 34
AfiI CCNNNNNNNGG 1 cut(s) 605
AgsI TTSAA 3 cut(s) 20, 157, 295
AluBI AGCT 5 cut(s) 97, 568, 635, 639, 719
AluI AGCT 5 cut(s) 97, 568, 635, 639, 719
Alw26I GTCTC 1 cut(s) 64
AlwI GGATC 3 cut(s) 278, 590, 603
AoxI GGCC 1 cut(s) 249
ApeKI GCWGC 2 cut(s) 5, 661
ApoI RAATTY 1 cut(s) 547
AspS9I GGNCC 2 cut(s) 28, 229
AsuC2I CCSGG 1 cut(s) 210
AsuHPI GGTGA 3 cut(s) 173, 224, 592
AsuNHI GCTAGC 1 cut(s) 635
AvaII GGWCC 2 cut(s) 28, 229
BalI TGGCCA 1 cut(s) 251
BamHI GGATCC 1 cut(s) 595
BbvI GCAGC 2 cut(s) 17, 648
BccI CCATC 4 cut(s) 295, 371, 383, 428
BceAI ACGGC 1 cut(s) 65
BclI TGATCA 1 cut(s) 274
BcnI CCSGG 1 cut(s) 210
BcoDI GTCTC 1 cut(s) 64
BfaI CTAG 4 cut(s) 221, 516, 552, 636
BisI GCNGC 2 cut(s) 6, 662
BlsI GCNGC 2 cut(s) 7, 663
Bme1390I CCNGG 1 cut(s) 210
Bme18I GGWCC 2 cut(s) 28, 229
BmgT120I GGNCC 2 cut(s) 28, 229
BmiI GGNNCC 3 cut(s) 231, 394, 597
BmrFI CCNGG 1 cut(s) 210
BmrI ACTGGG 1 cut(s) 393
BmsI GCATC 1 cut(s) 342
BmtI GCTAGC 1 cut(s) 639
BmuI ACTGGG 1 cut(s) 393
BoxI GACNNNNGTC 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 77
BpuMI CCSGG 1 cut(s) 210
BsaBI GATNNNNATC 1 cut(s) 20
BsaHI GRCGYC 1 cut(s) 78
BsaXI ACNNNNNCTCC 4 cut(s) 118, 148, 409, 439
Bsc4I CCNNNNNNNGG 1 cut(s) 605
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 2 cut(s) 109, 399
Bse3DI GCAATG 1 cut(s) 679
Bse8I GATNNNNATC 1 cut(s) 20
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 1 cut(s) 605
BseMI GCAATG 1 cut(s) 679
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 2 cut(s) 109, 399
BseXI GCAGC 2 cut(s) 17, 648
BshFI GGCC 1 cut(s) 251
BsiSI CCGG 2 cut(s) 42, 209
BslFI GGGAC 2 cut(s) 215, 623
BslI CCNNNNNNNGG 1 cut(s) 605
BsmAI GTCTC 1 cut(s) 64
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 2 cut(s) 215, 623
BsnI GGCC 1 cut(s) 251
Bsp143I GATC 3 cut(s) 274, 283, 595
BspACI CCGC 3 cut(s) 123, 336, 466
BspANI GGCC 1 cut(s) 251
BspCNI CTCAG 1 cut(s) 74
BspLI GGNNCC 3 cut(s) 231, 394, 597
BspOI GCTAGC 1 cut(s) 639
BspPI GGATC 3 cut(s) 278, 590, 603
BsrDI GCAATG 1 cut(s) 679
BsrFI RCCGGY 1 cut(s) 41
BsrI ACTGG 2 cut(s) 109, 399
BssAI RCCGGY 1 cut(s) 41
BssMI GATC 3 cut(s) 274, 283, 595
BssNI GRCGYC 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 58
Bst6I CTCTTC 2 cut(s) 155, 464
BstACI GRCGYC 1 cut(s) 78
BstC8I GCNNGC 3 cut(s) 637, 706, 721
BstDEI CTNAG 1 cut(s) 61
BstKTI GATC 3 cut(s) 277, 286, 598
BstMAI GTCTC 1 cut(s) 64
BstMBI GATC 3 cut(s) 274, 283, 595
BstMWI GCNNNNNNNGC 2 cut(s) 335, 645
BstNSI RCATGY 1 cut(s) 619
BstPAI GACNNNNGTC 1 cut(s) 56
BstSCI CCNGG 1 cut(s) 208
BstV1I GCAGC 2 cut(s) 17, 648
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
BsuRI GGCC 1 cut(s) 251
BtsIMutI CAGTG 2 cut(s) 406, 708
Cac8I GCNNGC 3 cut(s) 637, 706, 721
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 2 cut(s) 28, 229
CseI GACGC 1 cut(s) 86
Csp6I GTAC 1 cut(s) 33
CviAII CATG 3 cut(s) 316, 339, 616
CviQI GTAC 1 cut(s) 33
DdeI CTNAG 1 cut(s) 61
DpnI GATC 3 cut(s) 276, 285, 597
DpnII GATC 3 cut(s) 274, 283, 595
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 2 cut(s) 155, 464
EarI CTCTTC 2 cut(s) 155, 464
Eco47I GGWCC 2 cut(s) 28, 229
EcoRI GAATTC 1 cut(s) 547
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 3 cut(s) 319, 342, 619
FaiI YATR 8 cut(s) 317, 340, 350, 367, 413, 506, 601, 617
FaqI GGGAC 2 cut(s) 215, 623
FatI CATG 3 cut(s) 315, 338, 615
FauI CCCGC 1 cut(s) 130
FbaI TGATCA 1 cut(s) 274
Fnu4HI GCNGC 2 cut(s) 6, 662
Fsp4HI GCNGC 2 cut(s) 6, 662
FspBI CTAG 4 cut(s) 221, 516, 552, 636
GluI GCNGC 2 cut(s) 6, 662
HaeIII GGCC 1 cut(s) 251
HapII CCGG 2 cut(s) 42, 209
HgaI GACGC 1 cut(s) 86
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 3 cut(s) 319, 342, 619
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HinfI GANTC 4 cut(s) 10, 16, 181, 575
HpaII CCGG 2 cut(s) 42, 209
HphI GGTGA 3 cut(s) 173, 224, 592
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 6 cut(s) 15, 88, 261, 283, 346, 595
Hpy188III TCNNGA 2 cut(s) 536, 552
Hpy8I GTNNAC 1 cut(s) 73
Hpy99I CGWCG 2 cut(s) 80, 728
HpyAV CCTTC 2 cut(s) 124, 549
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 1 cut(s) 726
HpyCH4V TGCA 3 cut(s) 178, 355, 615
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 645
HpyF3I CTNAG 1 cut(s) 61
HpySE526I ACGT 1 cut(s) 726
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 3 cut(s) 319, 342, 619
Ksp22I TGATCA 1 cut(s) 274
Kzo9I GATC 3 cut(s) 274, 283, 595
LmnI GCTCC 2 cut(s) 317, 701
LpnPI CCDG 8 cut(s) 55, 122, 212, 222, 278, 341, 412, 591
Lsp1109I GCAGC 2 cut(s) 17, 648
LweI GCATC 1 cut(s) 342
MaeI CTAG 4 cut(s) 221, 516, 552, 636
MaeII ACGT 1 cut(s) 726
MalI GATC 3 cut(s) 276, 285, 597
MboI GATC 3 cut(s) 274, 283, 595
MboII GAAGA 4 cut(s) 142, 145, 481, 639
MflI RGATCY 1 cut(s) 595
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 3 cut(s) 139, 547, 583
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 3 cut(s) 4, 190, 584
MmeI TCCRAC 1 cut(s) 409
Mox20I TGGCCA 1 cut(s) 251
MscI TGGCCA 1 cut(s) 251
MseI TTAA 3 cut(s) 204, 240, 736
Msp20I TGGCCA 1 cut(s) 251
MspI CCGG 2 cut(s) 42, 209
MspR9I CCNGG 1 cut(s) 210
MwoI GCNNNNNNNGC 2 cut(s) 335, 645
NciI CCSGG 1 cut(s) 210
NdeII GATC 3 cut(s) 274, 283, 595
NheI GCTAGC 1 cut(s) 635
NlaIII CATG 3 cut(s) 319, 342, 619
NlaIV GGNNCC 3 cut(s) 231, 394, 597
NspI RCATGY 1 cut(s) 619
PfeI GAWTC 1 cut(s) 16
PflMI CCANNNNNTGG 1 cut(s) 605
PkrI GCNGC 2 cut(s) 7, 663
PleI GAGTC 3 cut(s) 4, 189, 583
PpsI GAGTC 3 cut(s) 4, 189, 583
PshAI GACNNNNGTC 1 cut(s) 56
PspN4I GGNNCC 3 cut(s) 231, 394, 597
PspPI GGNCC 2 cut(s) 28, 229
PsuI RGATCY 1 cut(s) 595
RsaI GTAC 1 cut(s) 34
RsaNI GTAC 1 cut(s) 33
SaqAI TTAA 3 cut(s) 204, 240, 736
SatI GCNGC 2 cut(s) 6, 662
Sau3AI GATC 3 cut(s) 274, 283, 595
Sau96I GGNCC 2 cut(s) 28, 229
SchI GAGTC 3 cut(s) 4, 190, 584
ScrFI CCNGG 1 cut(s) 210
SfaNI GCATC 1 cut(s) 342
SinI GGWCC 2 cut(s) 28, 229
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 3 cut(s) 139, 547, 583
SsiI CCGC 3 cut(s) 123, 336, 466
SspMI CTAG 4 cut(s) 221, 516, 552, 636
StyD4I CCNGG 1 cut(s) 208
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 1 cut(s) 729
TaqI TCGA 3 cut(s) 24, 184, 383
TasI AATT 3 cut(s) 139, 547, 583
TfiI GAWTC 1 cut(s) 16
Tru1I TTAA 3 cut(s) 204, 240, 736
Tru9I TTAA 3 cut(s) 204, 240, 736
TscAI CASTG 2 cut(s) 406, 715
TseI GCWGC 2 cut(s) 5, 661
TspDTI ATGAA 1 cut(s) 555
TspGWI ACGGA 2 cut(s) 20, 58
TspRI CASTG 2 cut(s) 406, 715
Van91I CCANNNNNTGG 1 cut(s) 605
VpaK11BI GGWCC 2 cut(s) 28, 229
XapI RAATTY 1 cut(s) 547
XbaI TCTAGA 1 cut(s) 551
XceI RCATGY 1 cut(s) 619
XspI CTAG 4 cut(s) 221, 516, 552, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.