RchiOBHm_Chr7g0190621

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
N/A
Physical Location & Seq
Reverse (-)
9634847 .. 9637425
2579 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 396 bp
ATGGTTTATGGAACCATTGGTGTACGGAGAATATCCAAAAAGTATGAAAGAGATGGTGAAGGAAAGGCTGCCTACTTTTTCTGCAGAAGAGAAAACTTTTTTTTTTTGGATGGAACAGAAGAGAAAACTTTGATCAAGGGAAGTTATGATTTTGTTGGAATCAATTACCATAAATCATTTTATGGCCGAGCCAAGGCGGCAAGGCTAAGAGGAAGAGGAGTCAGACAAGGGTACTTTGTTTGGACTCTGTTTGACAGCTTTGAATGGGGTATGGGGCTGACGAAGAGACTTGGGCTTTACTACATTGATTTCAATGACAATTTCAACCGGATCCCCAATCTTTCTGTTAAGTGGTTCCAAGCTTTCCTTAAAAGCAATCAGACCCGATCGAGTTAA

Protein Analysis

131

Amino Acids

15.49

Weight (kDa)

9.95

Isoelectric Point (pI)

25.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 54 - 127 4e-14 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 197
AclWI GGATC 2 cut(s) 325, 338
AcoI YGGCCR 1 cut(s) 184
AfaI GTAC 2 cut(s) 24, 233
AfiI CCNNNNNNNGG 1 cut(s) 193
AgsI TTSAA 3 cut(s) 263, 313, 325
AluBI AGCT 2 cut(s) 258, 362
AluI AGCT 2 cut(s) 258, 362
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 2 cut(s) 325, 338
AoxI GGCC 1 cut(s) 184
ApeKI GCWGC 1 cut(s) 68
AsuHPI GGTGA 1 cut(s) 68
BamHI GGATCC 1 cut(s) 330
BbvI GCAGC 1 cut(s) 55
BccI CCATC 2 cut(s) 47, 104
BclI TGATCA 1 cut(s) 132
BcoDI GTCTC 1 cut(s) 280
BfmI CTRYAG 1 cut(s) 82
BglI GCCNNNNNGGC 1 cut(s) 197
BisI GCNGC 2 cut(s) 69, 198
BlsI GCNGC 2 cut(s) 70, 199
BmiI GGNNCC 3 cut(s) 13, 332, 356
BsaJI CCNNGG 1 cut(s) 192
BsaWI WCCGGW 1 cut(s) 327
Bsc4I CCNNNNNNNGG 1 cut(s) 193
BseDI CCNNGG 1 cut(s) 192
BseGI GGATG 1 cut(s) 115
BseLI CCNNNNNNNGG 1 cut(s) 193
BseRI GAGGAG 1 cut(s) 231
BseXI GCAGC 1 cut(s) 55
Bsh1285I CGRYCG 1 cut(s) 389
BshFI GGCC 1 cut(s) 186
BsiEI CGRYCG 1 cut(s) 389
BsiSI CCGG 1 cut(s) 328
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 1 cut(s) 280
BsnI GGCC 1 cut(s) 186
Bsp143I GATC 3 cut(s) 132, 330, 386
BspACI CCGC 1 cut(s) 197
BspANI GGCC 1 cut(s) 186
BspLI GGNNCC 3 cut(s) 13, 332, 356
BspMAI CTGCAG 1 cut(s) 86
BspPI GGATC 2 cut(s) 325, 338
BssECI CCNNGG 1 cut(s) 192
BssMI GATC 3 cut(s) 132, 330, 386
BssT1I CCWWGG 1 cut(s) 192
Bst6I CTCTTC 4 cut(s) 82, 114, 208, 278
BstDEI CTNAG 1 cut(s) 206
BstF5I GGATG 1 cut(s) 115
BstKTI GATC 3 cut(s) 135, 333, 389
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 3 cut(s) 132, 330, 386
BstMCI CGRYCG 1 cut(s) 389
BstMWI GCNNNNNNNGC 1 cut(s) 197
BstSFI CTRYAG 1 cut(s) 82
BstV1I GCAGC 1 cut(s) 55
BstX2I RGATCY 1 cut(s) 330
BstYI RGATCY 1 cut(s) 330
BsuRI GGCC 1 cut(s) 186
BtsCI GGATG 1 cut(s) 115
Csp6I GTAC 2 cut(s) 23, 232
CviJI RGCY 8 cut(s) 68, 186, 191, 205, 258, 277, 295, 362
CviKI_1 RGCY 8 cut(s) 68, 186, 191, 205, 258, 277, 295, 362
CviQI GTAC 2 cut(s) 23, 232
DdeI CTNAG 1 cut(s) 206
DpnI GATC 3 cut(s) 134, 332, 388
DpnII GATC 3 cut(s) 132, 330, 386
EaeI YGGCCR 1 cut(s) 184
Eam1104I CTCTTC 4 cut(s) 82, 114, 208, 278
EarI CTCTTC 4 cut(s) 82, 114, 208, 278
Eco130I CCWWGG 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 192
ErhI CCWWGG 1 cut(s) 192
FaiI YATR 6 cut(s) 9, 45, 147, 171, 183, 272
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FbaI TGATCA 1 cut(s) 132
Fnu4HI GCNGC 2 cut(s) 69, 198
FokI GGATG 1 cut(s) 122
Fsp4HI GCNGC 2 cut(s) 69, 198
GluI GCNGC 2 cut(s) 69, 198
HaeIII GGCC 1 cut(s) 186
HapII CCGG 1 cut(s) 328
HindIII AAGCTT 1 cut(s) 360
HinfI GANTC 3 cut(s) 159, 219, 244
HpaII CCGG 1 cut(s) 328
HphI GGTGA 1 cut(s) 68
Hpy166II GTNNAC 1 cut(s) 23
Hpy188I TCNGA 2 cut(s) 224, 381
Hpy8I GTNNAC 1 cut(s) 23
HpyAV CCTTC 1 cut(s) 53
HpyCH4V TGCA 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 1 cut(s) 197
HpyF3I CTNAG 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 132
Kzo9I GATC 3 cut(s) 132, 330, 386
LpnPI CCDG 1 cut(s) 341
Lsp1109I GCAGC 1 cut(s) 55
MalI GATC 3 cut(s) 134, 332, 388
MboI GATC 3 cut(s) 132, 330, 386
MboII GAAGA 4 cut(s) 99, 131, 225, 295
MflI RGATCY 1 cut(s) 330
MluCI AATT 2 cut(s) 163, 319
MlyI GAGTC 2 cut(s) 228, 238
MmeI TCCRAC 1 cut(s) 136
MnlI CCTC 2 cut(s) 203, 209
MseI TTAA 3 cut(s) 348, 369, 394
MspI CCGG 1 cut(s) 328
MwoI GCNNNNNNNGC 1 cut(s) 197
NdeII GATC 3 cut(s) 132, 330, 386
NlaIV GGNNCC 3 cut(s) 13, 332, 356
NmeAIII GCCGAG 1 cut(s) 212
PfeI GAWTC 1 cut(s) 159
PkrI GCNGC 2 cut(s) 70, 199
Ple19I CGATCG 1 cut(s) 389
PleI GAGTC 2 cut(s) 227, 238
PpsI GAGTC 2 cut(s) 227, 238
PspN4I GGNNCC 3 cut(s) 13, 332, 356
PstI CTGCAG 1 cut(s) 86
PsuI RGATCY 1 cut(s) 330
PvuI CGATCG 1 cut(s) 389
RsaI GTAC 2 cut(s) 24, 233
RsaNI GTAC 2 cut(s) 23, 232
SaqAI TTAA 3 cut(s) 348, 369, 394
SatI GCNGC 2 cut(s) 69, 198
Sau3AI GATC 3 cut(s) 132, 330, 386
SchI GAGTC 2 cut(s) 228, 238
SetI ASST 2 cut(s) 260, 364
SfcI CTRYAG 1 cut(s) 82
SgeI CNNG 8 cut(s) 148, 200, 205, 213, 239, 302, 340, 371
Sse9I AATT 2 cut(s) 163, 319
SsiI CCGC 1 cut(s) 197
StyI CCWWGG 1 cut(s) 192
TaqI TCGA 1 cut(s) 389
TasI AATT 2 cut(s) 163, 319
TauI GCSGC 1 cut(s) 200
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 3 cut(s) 348, 369, 394
Tru9I TTAA 3 cut(s) 348, 369, 394
TseI GCWGC 1 cut(s) 68
TspDTI ATGAA 1 cut(s) 60
TspGWI ACGGA 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.