RchiOBHm_Chr7g0219151

Iron-sulfur assembly protein IscA-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
N/A
Physical Location & Seq
Reverse (-)
37501320 .. 37502512
1193 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 327 bp
ATGCGGCGGCTGCTAGAGCAGCGCCAGCGGTCGTATCTGAAGCTCGGGGTTAAGGCGCGTGGCTGCAACGGCTTGTCGTACACTCTCAATTACGCAGGTGAGGCTTGCCGAATGCCGATGATAAGGGAAAGTTCGATGAGTTGGTTGAAGATATTGATCGATCCAAAGGCTCTGATGCATGTTATTGGAACCAAGATGGATTTTGTGGATGACAAACTCAGGTCTGAGTTTATATTTATCAATCCCAACTCAAAAGGACAATGCGGCTGTGGTGAATCTTTTATGACAACAGGTAGTACTGGAGCTACAAAGCAAAGCAGCCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fe-S_biosyn

Protein Analysis

108

Amino Acids

12.09

Weight (kDa)

9.55

Isoelectric Point (pI)

55.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-S_biosyn PF01521 3 - 92 9.8e-08 Iron-sulphur cluster biosynthesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 86
Acc36I ACCTGC 1 cut(s) 86
AccII CGCG 1 cut(s) 58
AciI CCGC 4 cut(s) 4, 7, 28, 264
AclWI GGATC 1 cut(s) 155
AcuI CTGAAG 1 cut(s) 59
AfaI GTAC 2 cut(s) 80, 298
AgsI TTSAA 1 cut(s) 148
AluBI AGCT 2 cut(s) 43, 305
AluI AGCT 2 cut(s) 43, 305
AlwI GGATC 1 cut(s) 155
Ama87I CYCGRG 1 cut(s) 44
ApeKI GCWGC 4 cut(s) 10, 19, 63, 318
AspLEI GCGC 2 cut(s) 24, 58
AsuHPI GGTGA 2 cut(s) 110, 284
AvaI CYCGRG 1 cut(s) 44
BbvI GCAGC 2 cut(s) 31, 50
BccI CCATC 1 cut(s) 190
BceAI ACGGC 1 cut(s) 85
BfaI CTAG 1 cut(s) 14
BfoI RGCGCY 1 cut(s) 25
BfuAI ACCTGC 1 cut(s) 86
BisI GCNGC 7 cut(s) 5, 8, 11, 20, 64, 265, 319
BlsI GCNGC 7 cut(s) 6, 9, 12, 21, 65, 266, 320
BmcAI AGTACT 1 cut(s) 298
BmeT110I CYCGRG 1 cut(s) 44
BmiI GGNNCC 1 cut(s) 190
BmsI GCATC 1 cut(s) 165
BpmI CTGGAG 1 cut(s) 321
Bsa29I ATCGAT 1 cut(s) 159
BsaBI GATNNNNATC 1 cut(s) 155
Bse1I ACTGG 1 cut(s) 304
Bse8I GATNNNNATC 1 cut(s) 155
BseCI ATCGAT 1 cut(s) 159
BseGI GGATG 1 cut(s) 214
BseJI GATNNNNATC 1 cut(s) 155
BseMII CTCAG 2 cut(s) 216, 232
BseNI ACTGG 1 cut(s) 304
BseXI GCAGC 2 cut(s) 31, 50
Bsh1236I CGCG 1 cut(s) 58
Bsh1285I CGRYCG 1 cut(s) 32
BshVI ATCGAT 1 cut(s) 159
BsiEI CGRYCG 1 cut(s) 32
BsiHKCI CYCGRG 1 cut(s) 44
BsmI GAATGC 1 cut(s) 117
BsoBI CYCGRG 1 cut(s) 44
Bsp143I GATC 2 cut(s) 156, 160
BspACI CCGC 4 cut(s) 4, 7, 28, 264
BspCNI CTCAG 2 cut(s) 217, 231
BspDI ATCGAT 1 cut(s) 159
BspFNI CGCG 1 cut(s) 58
BspLI GGNNCC 1 cut(s) 190
BspMI ACCTGC 1 cut(s) 86
BspPI GGATC 1 cut(s) 155
BsrI ACTGG 1 cut(s) 304
BssMI GATC 2 cut(s) 156, 160
BstC8I GCNNGC 2 cut(s) 26, 106
BstDEI CTNAG 2 cut(s) 218, 225
BstF5I GGATG 1 cut(s) 214
BstFNI CGCG 1 cut(s) 58
BstH2I RGCGCY 1 cut(s) 25
BstHHI GCGC 2 cut(s) 24, 58
BstKTI GATC 2 cut(s) 159, 163
BstMBI GATC 2 cut(s) 156, 160
BstMCI CGRYCG 1 cut(s) 32
BstMWI GCNNNNNNNGC 6 cut(s) 10, 16, 19, 25, 69, 101
BstNSI RCATGY 1 cut(s) 182
BstUI CGCG 1 cut(s) 58
BstV1I GCAGC 2 cut(s) 31, 50
Bsu15I ATCGAT 1 cut(s) 159
BsuTUI ATCGAT 1 cut(s) 159
BtsCI GGATG 1 cut(s) 214
BveI ACCTGC 1 cut(s) 86
Cac8I GCNNGC 2 cut(s) 26, 106
CfoI GCGC 2 cut(s) 24, 58
ClaI ATCGAT 1 cut(s) 159
Csp6I GTAC 2 cut(s) 79, 297
CviAII CATG 1 cut(s) 179
CviJI RGCY 9 cut(s) 10, 43, 63, 72, 104, 170, 267, 305, 321
CviKI_1 RGCY 9 cut(s) 10, 43, 63, 72, 104, 170, 267, 305, 321
CviQI GTAC 2 cut(s) 79, 297
DdeI CTNAG 2 cut(s) 218, 225
DpnI GATC 2 cut(s) 158, 162
DpnII GATC 2 cut(s) 156, 160
Eco57I CTGAAG 1 cut(s) 59
Eco88I CYCGRG 1 cut(s) 44
EcoT22I ATGCAT 1 cut(s) 180
FaeI CATG 1 cut(s) 182
FaiI YATR 3 cut(s) 180, 233, 284
FatI CATG 1 cut(s) 178
Fnu4HI GCNGC 7 cut(s) 5, 8, 11, 20, 64, 265, 319
FokI GGATG 1 cut(s) 221
Fsp4HI GCNGC 7 cut(s) 5, 8, 11, 20, 64, 265, 319
FspBI CTAG 1 cut(s) 14
GlaI GCGC 2 cut(s) 23, 57
GluI GCNGC 7 cut(s) 5, 8, 11, 20, 64, 265, 319
GsuI CTGGAG 1 cut(s) 321
HaeII RGCGCY 1 cut(s) 25
HhaI GCGC 2 cut(s) 24, 58
Hin1II CATG 1 cut(s) 182
Hin6I GCGC 2 cut(s) 22, 56
HinP1I GCGC 2 cut(s) 22, 56
HinfI GANTC 1 cut(s) 275
HphI GGTGA 2 cut(s) 110, 284
Hpy166II GTNNAC 1 cut(s) 81
Hpy188I TCNGA 3 cut(s) 39, 174, 226
Hpy8I GTNNAC 1 cut(s) 81
HpyCH4V TGCA 2 cut(s) 66, 178
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 16, 19, 25, 69, 101
HpyF3I CTNAG 2 cut(s) 218, 225
Hsp92II CATG 1 cut(s) 182
HspAI GCGC 2 cut(s) 22, 56
Kzo9I GATC 2 cut(s) 156, 160
LmnI GCTCC 1 cut(s) 302
LpnPI CCDG 5 cut(s) 38, 81, 205, 276, 285
Lsp1109I GCAGC 2 cut(s) 31, 50
LweI GCATC 1 cut(s) 165
MaeI CTAG 1 cut(s) 14
MalI GATC 2 cut(s) 158, 162
MboI GATC 2 cut(s) 156, 160
MboII GAAGA 1 cut(s) 160
MluCI AATT 1 cut(s) 88
MnlI CCTC 1 cut(s) 94
Mph1103I ATGCAT 1 cut(s) 180
MseI TTAA 1 cut(s) 51
MspA1I CMGCKG 1 cut(s) 28
Mva1269I GAATGC 1 cut(s) 117
MvnI CGCG 1 cut(s) 58
MwoI GCNNNNNNNGC 6 cut(s) 10, 16, 19, 25, 69, 101
NdeII GATC 2 cut(s) 156, 160
NlaIII CATG 1 cut(s) 182
NlaIV GGNNCC 1 cut(s) 190
NsiI ATGCAT 1 cut(s) 180
NspI RCATGY 1 cut(s) 182
PaqCI CACCTGC 1 cut(s) 86
PctI GAATGC 1 cut(s) 117
PfeI GAWTC 1 cut(s) 275
PkrI GCNGC 7 cut(s) 6, 9, 12, 21, 65, 266, 320
PspN4I GGNNCC 1 cut(s) 190
RsaI GTAC 2 cut(s) 80, 298
RsaNI GTAC 2 cut(s) 79, 297
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 7 cut(s) 5, 8, 11, 20, 64, 265, 319
Sau3AI GATC 2 cut(s) 156, 160
ScaI AGTACT 1 cut(s) 298
SetI ASST 5 cut(s) 45, 100, 224, 295, 307
SfaNI GCATC 1 cut(s) 165
Sse9I AATT 1 cut(s) 88
SsiI CCGC 4 cut(s) 4, 7, 28, 264
SspMI CTAG 1 cut(s) 14
TaqI TCGA 2 cut(s) 134, 159
TasI AATT 1 cut(s) 88
TatI WGTACW 1 cut(s) 296
TauI GCSGC 3 cut(s) 7, 10, 267
TfiI GAWTC 1 cut(s) 275
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TseI GCWGC 4 cut(s) 10, 19, 63, 318
XceI RCATGY 1 cut(s) 182
XspI CTAG 1 cut(s) 14
ZrmI AGTACT 1 cut(s) 298
Zsp2I ATGCAT 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.