RchiOBHm_Chr7g0228191

Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
N/A
Physical Location & Seq
Reverse (-)
51543082 .. 51544742
1661 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 561 bp
ATGTCTGTAGTAAATTTACTGTCTTTTTACCTTGCGCATTTCCAGCTGGTCGGTGTATTTTATCCATACAACCGAGGAGCTCTGTTCACTGCTCTTGTGGTCAGAAATTTATTACTTACTGGCTGCCTTTTTTGTGGGTCTCTGTTCCTGACATTCTGCTTCCTTAACACAGTTTACATTGAGCTTTACTACATATTTGCCAGTGTCTGGGGTCACAGGATTTACACCATCTACAGCATTCTGTTTATTGTTTTTCTTATTCTTTTGCTTGTCACTGTCTTTATAACTGTGGCTTTGACCTACTTCCAACTTGCTGCTGAAGATCACGAGTGGTGGCCTGATTTGTTGTTGATGTGTACATCGTTCTTCTTTGGTTACATGGCTTGCATTTGCTATGGCTTCTTTCTCATGCTTGGCACTGTTGGATTTCGTGCTGCTTTGCTCTTTGTTTGTCACATTTACAAGTCTATCAAGCGGGTTAGCTGGATCTTTTTGGTGAATTGGTACTTGTTTCTTCCTTTAGAACATTGGAAAAAGTCTACGATTTTTGGTAGAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP70

Protein Analysis

186

Amino Acids

21.9

Weight (kDa)

8.85

Isoelectric Point (pI)

31.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 57 - 135 1.2e-17 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 284
Acc16I TGCGCA 1 cut(s) 36
AccB7I CCANNNNNTGG 1 cut(s) 207
AccI GTMKAC 1 cut(s) 539
AciI CCGC 1 cut(s) 475
AclWI GGATC 1 cut(s) 494
AcsI RAATTY 2 cut(s) 13, 106
AcuI CTGAAG 1 cut(s) 339
AfaI GTAC 2 cut(s) 358, 506
AfiI CCNNNNNNNGG 1 cut(s) 207
AluBI AGCT 4 cut(s) 46, 80, 184, 483
AluI AGCT 4 cut(s) 46, 80, 184, 483
Alw21I GWGCWC 1 cut(s) 82
Alw26I GTCTC 1 cut(s) 144
AlwI GGATC 1 cut(s) 494
AlwNI CAGNNNCTG 1 cut(s) 207
AoxI GGCC 1 cut(s) 335
ApeKI GCWGC 3 cut(s) 123, 314, 434
ApoI RAATTY 2 cut(s) 13, 106
AspLEI GCGC 1 cut(s) 37
AsuHPI GGTGA 1 cut(s) 508
BanII GRGCYC 1 cut(s) 82
BauI CACGAG 1 cut(s) 326
Bbv12I GWGCWC 1 cut(s) 82
BbvI GCAGC 3 cut(s) 110, 301, 421
BccI CCATC 1 cut(s) 236
BcoDI GTCTC 1 cut(s) 144
BfmI CTRYAG 2 cut(s) 6, 232
BisI GCNGC 3 cut(s) 124, 315, 435
BlsI GCNGC 3 cut(s) 125, 316, 436
BsaI GGTCTC 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 73
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse1I ACTGG 2 cut(s) 124, 201
BseDI CCNNGG 1 cut(s) 73
BseLI CCNNNNNNNGG 1 cut(s) 207
BseNI ACTGG 2 cut(s) 124, 201
BseRI GAGGAG 1 cut(s) 90
BseXI GCAGC 3 cut(s) 110, 301, 421
BshFI GGCC 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 82
BslI CCNNNNNNNGG 1 cut(s) 207
BsmAI GTCTC 1 cut(s) 144
BsmI GAATGC 1 cut(s) 237
BsnI GGCC 1 cut(s) 337
Bso31I GGTCTC 1 cut(s) 144
Bsp1286I GDGCHC 1 cut(s) 82
Bsp1407I TGTACA 1 cut(s) 356
Bsp143I GATC 2 cut(s) 322, 486
BspACI CCGC 1 cut(s) 475
BspANI GGCC 1 cut(s) 337
BspPI GGATC 1 cut(s) 494
BspTNI GGTCTC 1 cut(s) 144
BsrGI TGTACA 1 cut(s) 356
BsrI ACTGG 2 cut(s) 124, 201
BssECI CCNNGG 1 cut(s) 73
BssMI GATC 2 cut(s) 322, 486
BssSI CACGAG 1 cut(s) 326
Bst2BI CACGAG 1 cut(s) 326
Bst4CI ACNGT 5 cut(s) 21, 172, 277, 289, 421
BstAUI TGTACA 1 cut(s) 356
BstC8I GCNNGC 1 cut(s) 385
BstHHI GCGC 1 cut(s) 37
BstKTI GATC 2 cut(s) 325, 489
BstMAI GTCTC 1 cut(s) 144
BstMBI GATC 2 cut(s) 322, 486
BstMWI GCNNNNNNNGC 1 cut(s) 43
BstSFI CTRYAG 2 cut(s) 6, 232
BstV1I GCAGC 3 cut(s) 110, 301, 421
BstX2I RGATCY 1 cut(s) 486
BstYI RGATCY 1 cut(s) 486
BsuRI GGCC 1 cut(s) 337
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 4 cut(s) 87, 208, 273, 417
Cac8I GCNNGC 1 cut(s) 385
CaiI CAGNNNCTG 1 cut(s) 207
CfoI GCGC 1 cut(s) 37
Csp6I GTAC 2 cut(s) 357, 505
CviAII CATG 2 cut(s) 379, 409
CviJI RGCY 9 cut(s) 46, 80, 123, 184, 293, 337, 383, 399, 483
CviKI_1 RGCY 9 cut(s) 46, 80, 123, 184, 293, 337, 383, 399, 483
CviQI GTAC 2 cut(s) 357, 505
DpnI GATC 2 cut(s) 324, 488
DpnII GATC 2 cut(s) 322, 486
Ecl136II GAGCTC 1 cut(s) 80
Eco24I GRGCYC 1 cut(s) 82
Eco31I GGTCTC 1 cut(s) 144
Eco53kI GAGCTC 1 cut(s) 80
Eco57I CTGAAG 1 cut(s) 339
EcoICRI GAGCTC 1 cut(s) 80
EcoT38I GRGCYC 1 cut(s) 82
FaeI CATG 2 cut(s) 382, 412
FaiI YATR 6 cut(s) 67, 194, 284, 380, 396, 410
FatI CATG 2 cut(s) 378, 408
FauI CCCGC 1 cut(s) 468
FblI GTMKAC 1 cut(s) 539
Fnu4HI GCNGC 3 cut(s) 124, 315, 435
FriOI GRGCYC 1 cut(s) 82
Fsp4HI GCNGC 3 cut(s) 124, 315, 435
FspI TGCGCA 1 cut(s) 36
GlaI GCGC 1 cut(s) 36
GluI GCNGC 3 cut(s) 124, 315, 435
HaeIII GGCC 1 cut(s) 337
HhaI GCGC 1 cut(s) 37
Hin1II CATG 2 cut(s) 382, 412
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HphI GGTGA 1 cut(s) 508
Hpy166II GTNNAC 4 cut(s) 87, 175, 357, 540
Hpy188I TCNGA 1 cut(s) 104
Hpy188III TCNNGA 2 cut(s) 148, 326
Hpy8I GTNNAC 4 cut(s) 87, 175, 357, 540
HpyAV CCTTC 1 cut(s) 549
HpyCH4III ACNGT 5 cut(s) 21, 172, 277, 289, 421
HpyCH4V TGCA 1 cut(s) 387
HpyF10VI GCNNNNNNNGC 1 cut(s) 43
Hsp92II CATG 2 cut(s) 382, 412
HspAI GCGC 1 cut(s) 35
Kzo9I GATC 2 cut(s) 322, 486
LmnI GCTCC 1 cut(s) 77
LpnPI CCDG 9 cut(s) 32, 56, 105, 161, 193, 202, 214, 351, 469
Lsp1109I GCAGC 3 cut(s) 110, 301, 421
MaeIII GTNAC 4 cut(s) 212, 271, 374, 452
MalI GATC 2 cut(s) 324, 488
MboI GATC 2 cut(s) 322, 486
MboII GAAGA 3 cut(s) 332, 358, 506
MflI RGATCY 1 cut(s) 486
MhlI GDGCHC 1 cut(s) 82
MluCI AATT 3 cut(s) 13, 106, 499
MmeI TCCRAC 2 cut(s) 331, 403
MnlI CCTC 1 cut(s) 68
MseI TTAA 1 cut(s) 165
MspA1I CMGCKG 1 cut(s) 46
Mva1269I GAATGC 1 cut(s) 237
MwoI GCNNNNNNNGC 1 cut(s) 43
NdeII GATC 2 cut(s) 322, 486
NlaIII CATG 2 cut(s) 382, 412
NmuCI GTSAC 3 cut(s) 212, 271, 452
NsbI TGCGCA 1 cut(s) 36
PctI GAATGC 1 cut(s) 237
PflMI CCANNNNNTGG 1 cut(s) 207
PkrI GCNGC 3 cut(s) 125, 316, 436
PsiI TTATAA 1 cut(s) 284
Psp124BI GAGCTC 1 cut(s) 82
PstNI CAGNNNCTG 1 cut(s) 207
PsuI RGATCY 1 cut(s) 486
PvuII CAGCTG 1 cut(s) 46
RsaI GTAC 2 cut(s) 358, 506
RsaNI GTAC 2 cut(s) 357, 505
SacI GAGCTC 1 cut(s) 82
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 3 cut(s) 124, 315, 435
Sau3AI GATC 2 cut(s) 322, 486
SduI GDGCHC 1 cut(s) 82
SetI ASST 7 cut(s) 33, 48, 82, 186, 302, 485, 560
SfcI CTRYAG 2 cut(s) 6, 232
Sse9I AATT 3 cut(s) 13, 106, 499
SsiI CCGC 1 cut(s) 475
SstI GAGCTC 1 cut(s) 82
TaaI ACNGT 5 cut(s) 21, 172, 277, 289, 421
TasI AATT 3 cut(s) 13, 106, 499
TatI WGTACW 1 cut(s) 356
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 4 cut(s) 94, 208, 280, 424
TseFI GTSAC 3 cut(s) 212, 271, 452
TseI GCWGC 3 cut(s) 123, 314, 434
Tsp45I GTSAC 3 cut(s) 212, 271, 452
TspRI CASTG 4 cut(s) 94, 208, 280, 424
Van91I CCANNNNNTGG 1 cut(s) 207
XapI RAATTY 2 cut(s) 13, 106
XmiI GTMKAC 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.