Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| TGT | PF01702 | 1 - 80 | 3e-31 | Queuine tRNA-ribosyltransferase |
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No orthologs found for this gene in the current database.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 157 |
| AclWI | GGATC | 1 cut(s) | 161 |
| AcuI | CTGAAG | 2 cut(s) | 143, 149 |
| AfaI | GTAC | 1 cut(s) | 82 |
| AflIII | ACRYGT | 2 cut(s) | 54, 77 |
| AluBI | AGCT | 4 cut(s) | 101, 127, 140, 209 |
| AluI | AGCT | 4 cut(s) | 101, 127, 140, 209 |
| AlwI | GGATC | 1 cut(s) | 161 |
| AxyI | CCTNAGG | 1 cut(s) | 111 |
| BbvCI | CCTCAGC | 1 cut(s) | 147 |
| BfaI | CTAG | 2 cut(s) | 204, 224 |
| BmsI | GCATC | 1 cut(s) | 226 |
| Bpu10I | CCTNAGC | 1 cut(s) | 147 |
| Bsa29I | ATCGAT | 1 cut(s) | 165 |
| BsaAI | YACGTR | 1 cut(s) | 80 |
| Bse21I | CCTNAGG | 1 cut(s) | 111 |
| Bse3DI | GCAATG | 1 cut(s) | 217 |
| BseCI | ATCGAT | 1 cut(s) | 165 |
| BseMI | GCAATG | 1 cut(s) | 217 |
| BseMII | CTCAG | 2 cut(s) | 102, 138 |
| BshVI | ATCGAT | 1 cut(s) | 165 |
| Bsp143I | GATC | 2 cut(s) | 166, 245 |
| BspACI | CCGC | 1 cut(s) | 157 |
| BspCNI | CTCAG | 2 cut(s) | 103, 139 |
| BspDI | ATCGAT | 1 cut(s) | 165 |
| BspPI | GGATC | 1 cut(s) | 161 |
| BsrDI | GCAATG | 1 cut(s) | 217 |
| BssMI | GATC | 2 cut(s) | 166, 245 |
| Bst4CI | ACNGT | 1 cut(s) | 65 |
| BstBAI | YACGTR | 1 cut(s) | 80 |
| BstC8I | GCNNGC | 1 cut(s) | 88 |
| BstDEI | CTNAG | 3 cut(s) | 111, 147, 249 |
| BstKTI | GATC | 2 cut(s) | 169, 248 |
| BstMBI | GATC | 2 cut(s) | 166, 245 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 47 |
| BstNSI | RCATGY | 1 cut(s) | 58 |
| BstX2I | RGATCY | 1 cut(s) | 245 |
| BstYI | RGATCY | 1 cut(s) | 245 |
| Bsu15I | ATCGAT | 1 cut(s) | 165 |
| Bsu36I | CCTNAGG | 1 cut(s) | 111 |
| BsuTUI | ATCGAT | 1 cut(s) | 165 |
| BtsI | GCAGTG | 1 cut(s) | 43 |
| BtsIMutI | CAGTG | 1 cut(s) | 43 |
| Cac8I | GCNNGC | 1 cut(s) | 88 |
| ClaI | ATCGAT | 1 cut(s) | 165 |
| Csp6I | GTAC | 1 cut(s) | 81 |
| CviAII | CATG | 1 cut(s) | 55 |
| CviJI | RGCY | 5 cut(s) | 101, 127, 140, 146, 209 |
| CviKI_1 | RGCY | 5 cut(s) | 101, 127, 140, 146, 209 |
| CviQI | GTAC | 1 cut(s) | 81 |
| DdeI | CTNAG | 3 cut(s) | 111, 147, 249 |
| DpnI | GATC | 2 cut(s) | 168, 247 |
| DpnII | GATC | 2 cut(s) | 166, 245 |
| EciI | GGCGGA | 1 cut(s) | 146 |
| Eco32I | GATATC | 1 cut(s) | 154 |
| Eco57I | CTGAAG | 2 cut(s) | 143, 149 |
| Eco81I | CCTNAGG | 1 cut(s) | 111 |
| EcoRV | GATATC | 1 cut(s) | 154 |
| FaeI | CATG | 1 cut(s) | 58 |
| FaiI | YATR | 8 cut(s) | 56, 60, 107, 143, 185, 190, 201, 242 |
| FatI | CATG | 1 cut(s) | 54 |
| FspBI | CTAG | 2 cut(s) | 204, 224 |
| Hin1II | CATG | 1 cut(s) | 58 |
| HpyCH4III | ACNGT | 1 cut(s) | 65 |
| HpyCH4IV | ACGT | 1 cut(s) | 79 |
| HpyCH4V | TGCA | 2 cut(s) | 36, 192 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 47 |
| HpyF3I | CTNAG | 3 cut(s) | 111, 147, 249 |
| HpySE526I | ACGT | 1 cut(s) | 79 |
| Hsp92II | CATG | 1 cut(s) | 58 |
| Kzo9I | GATC | 2 cut(s) | 166, 245 |
| LpnPI | CCDG | 1 cut(s) | 123 |
| LweI | GCATC | 1 cut(s) | 226 |
| MaeI | CTAG | 2 cut(s) | 204, 224 |
| MaeII | ACGT | 1 cut(s) | 79 |
| MaeIII | GTNAC | 1 cut(s) | 226 |
| MalI | GATC | 2 cut(s) | 168, 247 |
| MboI | GATC | 2 cut(s) | 166, 245 |
| MflI | RGATCY | 1 cut(s) | 245 |
| MluCI | AATT | 1 cut(s) | 196 |
| MmeI | TCCRAC | 1 cut(s) | 98 |
| MnlI | CCTC | 2 cut(s) | 106, 142 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 47 |
| NdeII | GATC | 2 cut(s) | 166, 245 |
| NlaIII | CATG | 1 cut(s) | 58 |
| NspI | RCATGY | 1 cut(s) | 58 |
| PciI | ACATGT | 1 cut(s) | 54 |
| Ppu21I | YACGTR | 1 cut(s) | 80 |
| PscI | ACATGT | 1 cut(s) | 54 |
| PsuI | RGATCY | 1 cut(s) | 245 |
| RsaI | GTAC | 1 cut(s) | 82 |
| RsaNI | GTAC | 1 cut(s) | 81 |
| Sau3AI | GATC | 2 cut(s) | 166, 245 |
| SetI | ASST | 7 cut(s) | 49, 82, 103, 112, 129, 142, 211 |
| SfaNI | GCATC | 1 cut(s) | 226 |
| SgeI | CNNG | 9 cut(s) | 67, 90, 99, 122, 186, 192, 205, 216, 236 |
| Sse9I | AATT | 1 cut(s) | 196 |
| SsiI | CCGC | 1 cut(s) | 157 |
| SspMI | CTAG | 2 cut(s) | 204, 224 |
| TaaI | ACNGT | 1 cut(s) | 65 |
| TaiI | ACGT | 1 cut(s) | 82 |
| TaqI | TCGA | 1 cut(s) | 165 |
| TasI | AATT | 1 cut(s) | 196 |
| TscAI | CASTG | 1 cut(s) | 43 |
| TspDTI | ATGAA | 1 cut(s) | 206 |
| TspRI | CASTG | 1 cut(s) | 43 |
| XceI | RCATGY | 1 cut(s) | 58 |
| XspI | CTAG | 2 cut(s) | 204, 224 |