RchiOBHm_Chr6g0244661

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
N/A
Physical Location & Seq
Reverse (-)
686268 .. 687629
1362 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 423 bp
ATGTTATTTGATTCAATCAAGGACGATGCTTTTCTGAAACAAACTTTTGTGCAGCATTTTACCACACTGAGGAAACAAGGTGCAATTTTGGTTGACAATCTTGAAATAGCCAATATTGAAGATGTTCTGGATTCTCATTCAAGTGGAGAATTTGATGCTATATTAGCTGAATTCAAAATAGCCTTGAATGAGTACCTAAGTGACCTAGTAAAATCTCCAGTGAAGTCCTTAAGGGAGGTGATAGAGTTCAACAAGAATCATTCAAAGGTGGAAAATATCAATGAATATGGGCAAGATGTCTTCGAATTAGCTGAGAAGACTAATGGGATGGGACCAAAAGAGCAGGAGGCATTGTCAAATTTAGAAAGACTATCTAGACAGGGGTTCAAGAAGTTGATGACCGATCATAGCCTAGAGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

140

Amino Acids

15.94

Weight (kDa)

4.89

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 149, 170, 358
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 1 cut(s) 69
AflII CTTAAG 1 cut(s) 229
AgsI TTSAA 9 cut(s) 15, 104, 119, 141, 175, 187, 250, 264, 388
AluBI AGCT 2 cut(s) 167, 311
AluI AGCT 2 cut(s) 167, 311
ApeKI GCWGC 1 cut(s) 52
ApoI RAATTY 3 cut(s) 149, 170, 358
Asp700I GAANNNNTTC 1 cut(s) 123
AspS9I GGNCC 1 cut(s) 332
AsuHPI GGTGA 1 cut(s) 250
AsuII TTCGAA 1 cut(s) 303
AvaII GGWCC 1 cut(s) 332
BanII GRGCYC 1 cut(s) 420
BbsI GAAGAC 2 cut(s) 292, 323
BbvI GCAGC 1 cut(s) 64
BccI CCATC 1 cut(s) 322
BfaI CTAG 4 cut(s) 206, 375, 413, 421
BfrI CTTAAG 1 cut(s) 229
BisI GCNGC 1 cut(s) 53
BlsI GCNGC 1 cut(s) 54
Bme18I GGWCC 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 332
BmiI GGNNCC 1 cut(s) 333
BmsI GCATC 2 cut(s) 16, 145
BpiI GAAGAC 2 cut(s) 292, 323
BpmI CTGGAG 1 cut(s) 201
Bpu14I TTCGAA 1 cut(s) 303
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse1I ACTGG 1 cut(s) 218
BseGI GGATG 1 cut(s) 333
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMII CTCAG 2 cut(s) 59, 303
BseNI ACTGG 1 cut(s) 218
BseXI GCAGC 1 cut(s) 64
BsgI GTGCAG 1 cut(s) 71
BslFI GGGAC 1 cut(s) 345
BslI CCNNNNNNNGG 1 cut(s) 69
BsmFI GGGAC 1 cut(s) 345
Bsp119I TTCGAA 1 cut(s) 303
Bsp1286I GDGCHC 1 cut(s) 420
Bsp143I GATC 1 cut(s) 403
BspCNI CTCAG 2 cut(s) 60, 304
BspLI GGNNCC 1 cut(s) 333
BspT104I TTCGAA 1 cut(s) 303
BspTI CTTAAG 1 cut(s) 229
BsrI ACTGG 1 cut(s) 218
BssMI GATC 1 cut(s) 403
BstAFI CTTAAG 1 cut(s) 229
BstBI TTCGAA 1 cut(s) 303
BstDEI CTNAG 3 cut(s) 68, 197, 312
BstF5I GGATG 1 cut(s) 333
BstKTI GATC 1 cut(s) 406
BstMBI GATC 1 cut(s) 403
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstV1I GCAGC 1 cut(s) 64
BstV2I GAAGAC 2 cut(s) 292, 323
BtsCI GGATG 1 cut(s) 333
BtsIMutI CAGTG 2 cut(s) 65, 225
Cfr13I GGNCC 1 cut(s) 332
Csp6I GTAC 1 cut(s) 193
CviJI RGCY 6 cut(s) 110, 167, 182, 311, 411, 418
CviKI_1 RGCY 6 cut(s) 110, 167, 182, 311, 411, 418
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 3 cut(s) 68, 197, 312
DpnI GATC 1 cut(s) 405
DpnII GATC 1 cut(s) 403
Eco24I GRGCYC 1 cut(s) 420
Eco47I GGWCC 1 cut(s) 332
EcoRI GAATTC 1 cut(s) 170
EcoT38I GRGCYC 1 cut(s) 420
FaiI YATR 3 cut(s) 161, 288, 408
FaqI GGGAC 1 cut(s) 345
Fnu4HI GCNGC 1 cut(s) 53
FokI GGATG 1 cut(s) 340
FriOI GRGCYC 1 cut(s) 420
Fsp4HI GCNGC 1 cut(s) 53
FspBI CTAG 4 cut(s) 206, 375, 413, 421
GluI GCNGC 1 cut(s) 53
GsuI CTGGAG 1 cut(s) 201
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 3 cut(s) 11, 131, 256
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 1 cut(s) 36
Hpy188III TCNNGA 4 cut(s) 101, 128, 375, 388
Hpy8I GTNNAC 1 cut(s) 94
HpyCH4V TGCA 2 cut(s) 52, 83
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 3 cut(s) 68, 197, 312
Kzo9I GATC 1 cut(s) 403
LpnPI CCDG 4 cut(s) 113, 231, 329, 365
Lsp1109I GCAGC 1 cut(s) 64
LweI GCATC 2 cut(s) 16, 145
MaeI CTAG 4 cut(s) 206, 375, 413, 421
MaeIII GTNAC 1 cut(s) 200
MalI GATC 1 cut(s) 405
MboI GATC 1 cut(s) 403
MboII GAAGA 3 cut(s) 131, 292, 328
MhlI GDGCHC 1 cut(s) 420
MluCI AATT 5 cut(s) 84, 149, 170, 305, 358
MnlI CCTC 3 cut(s) 63, 229, 340
MroXI GAANNNNTTC 1 cut(s) 123
MseI TTAA 1 cut(s) 230
MslI CAYNNNNRTG 1 cut(s) 141
MspCI CTTAAG 1 cut(s) 229
MwoI GCNNNNNNNGC 1 cut(s) 164
NdeII GATC 1 cut(s) 403
NlaIV GGNNCC 1 cut(s) 333
NmuCI GTSAC 1 cut(s) 200
NspV TTCGAA 1 cut(s) 303
PdmI GAANNNNTTC 1 cut(s) 123
PfeI GAWTC 3 cut(s) 11, 131, 256
PkrI GCNGC 1 cut(s) 54
PspN4I GGNNCC 1 cut(s) 333
PspPI GGNCC 1 cut(s) 332
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
RseI CAYNNNNRTG 1 cut(s) 141
SaqAI TTAA 1 cut(s) 230
SatI GCNGC 1 cut(s) 53
Sau3AI GATC 1 cut(s) 403
Sau96I GGNCC 1 cut(s) 332
SduI GDGCHC 1 cut(s) 420
SetI ASST 7 cut(s) 82, 169, 198, 207, 240, 270, 313
SfaNI GCATC 2 cut(s) 16, 145
SfuI TTCGAA 1 cut(s) 303
SinI GGWCC 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 141
SmlI CTYRAG 1 cut(s) 229
SmoI CTYRAG 1 cut(s) 229
Sse9I AATT 5 cut(s) 84, 149, 170, 305, 358
SspI AATATT 1 cut(s) 115
SspMI CTAG 4 cut(s) 206, 375, 413, 421
TaqI TCGA 1 cut(s) 303
TaqII GACCGA 1 cut(s) 416
TasI AATT 5 cut(s) 84, 149, 170, 305, 358
TfiI GAWTC 3 cut(s) 11, 131, 256
Tru1I TTAA 1 cut(s) 230
Tru9I TTAA 1 cut(s) 230
TscAI CASTG 2 cut(s) 72, 225
TseFI GTSAC 1 cut(s) 200
TseI GCWGC 1 cut(s) 52
Tsp45I GTSAC 1 cut(s) 200
TspDTI ATGAA 1 cut(s) 297
TspRI CASTG 2 cut(s) 72, 225
Vha464I CTTAAG 1 cut(s) 229
VpaK11BI GGWCC 1 cut(s) 332
XapI RAATTY 3 cut(s) 149, 170, 358
XbaI TCTAGA 1 cut(s) 374
XmnI GAANNNNTTC 1 cut(s) 123
XspI CTAG 4 cut(s) 206, 375, 413, 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.