RchiOBHm_Chr6g0259771

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
N/A
Physical Location & Seq
Reverse (-)
14992597 .. 14993442
846 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
ATGGTCTTTTCAGTTTTTTACTCTGAGCACCCAGCTTTCTCTCTCTGGGCACCCAGCATGAGGAAGCATCTACGATCACCTCCGGCAATATGGGTTACCCATCGGCCTCCTCCCCAAAGGCATCAAGGAATATTCCCTCGACGATTCCAGCGGCAAATTCCAGGTGTTTCTGCCCCAGCCTTGCCTGCCAAGCTCGAGAATCAGGTCCGGTACGATTTCAATGTCTCTGGGACTCTCACTTTCGGCCGAATCGCCGACTTATCCGGCGTCTCCGCTCAGGAACTCTTCCTCTGGTTTCCAGTTAAAGGTATCCGAGTTGATGTGCCCAGCTCCGGCCTCATTTACTTCGACGTCGGCGTTGTCGATAAGCAGTTCTCTTTGTCCCTCTTCGAGTCTCCGCCCTATTGCACTGCTCTTGATCCTTCTGATCCCTCCTCTCCTTCTTCTTCTTCCAATTCCCCATCTGAGCTCAAGCTCAAGGTAGATTCTGATTCTGATTCCAATTCCAATTCCAATTCTCAATTCGATTTCGTTGCTTCGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

181

Amino Acids

20.05

Weight (kDa)

6.9

Isoelectric Point (pI)

63.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 64 - 131 1.4e-17 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 354
AccB1I GGYRCC 1 cut(s) 49
AccBSI CCGCTC 1 cut(s) 275
AciI CCGC 3 cut(s) 151, 273, 398
AclWI GGATC 2 cut(s) 413, 422
AcoI YGGCCR 1 cut(s) 244
AcsI RAATTY 1 cut(s) 156
AcyI GRCGYC 2 cut(s) 267, 351
AfaI GTAC 1 cut(s) 212
AfiI CCNNNNNNNGG 3 cut(s) 60, 305, 332
AgsI TTSAA 1 cut(s) 220
AjnI CCWGG 1 cut(s) 160
AjuI GAANNNNNNNTTGG 2 cut(s) 506, 538
AluBI AGCT 5 cut(s) 35, 193, 330, 469, 475
AluI AGCT 5 cut(s) 35, 193, 330, 469, 475
Alw21I GWGCWC 2 cut(s) 30, 471
Alw26I GTCTC 3 cut(s) 229, 274, 399
AlwI GGATC 2 cut(s) 413, 422
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 3 cut(s) 104, 244, 334
ApoI RAATTY 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 205
AsuHPI GGTGA 1 cut(s) 69
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 1 cut(s) 205
BaeGI GKGCMC 2 cut(s) 52, 327
BanI GGYRCC 1 cut(s) 49
BanII GRGCYC 1 cut(s) 471
Bbv12I GWGCWC 2 cut(s) 30, 471
BccI CCATC 2 cut(s) 108, 469
BcgI CGANNNNNNTGC 1 cut(s) 515
BciT130I CCWGG 1 cut(s) 162
BciVI GTATCC 1 cut(s) 320
BcoDI GTCTC 3 cut(s) 229, 274, 399
BfuI GTATCC 1 cut(s) 320
BisI GCNGC 1 cut(s) 152
BlsI GCNGC 1 cut(s) 153
Bme1390I CCNGG 1 cut(s) 162
Bme18I GGWCC 1 cut(s) 205
BmeT110I CYCGRG 1 cut(s) 194
BmgT120I GGNCC 1 cut(s) 205
BmiI GGNNCC 1 cut(s) 51
BmrFI CCNGG 1 cut(s) 162
BmsI GCATC 2 cut(s) 76, 130
Bpu10I CCTNAGC 1 cut(s) 276
BpuEI CTTGAG 2 cut(s) 455, 461
BsaHI GRCGYC 2 cut(s) 267, 351
BsaWI WCCGGW 1 cut(s) 207
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 305, 332
Bse1I ACTGG 1 cut(s) 299
BseBI CCWGG 1 cut(s) 162
BseLI CCNNNNNNNGG 3 cut(s) 60, 305, 332
BseMII CTCAG 3 cut(s) 15, 290, 456
BseNI ACTGG 1 cut(s) 299
BseRI GAGGAG 2 cut(s) 99, 424
BseSI GKGCMC 2 cut(s) 52, 327
BseX3I CGGCCG 1 cut(s) 244
BseYI CCCAGC 4 cut(s) 31, 53, 175, 326
Bsh1285I CGRYCG 1 cut(s) 247
BshFI GGCC 3 cut(s) 106, 246, 336
BshNI GGYRCC 1 cut(s) 49
BsiEI CGRYCG 1 cut(s) 247
BsiHKAI GWGCWC 2 cut(s) 30, 471
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 4 cut(s) 83, 208, 264, 333
BslFI GGGAC 2 cut(s) 244, 367
BslI CCNNNNNNNGG 3 cut(s) 60, 305, 332
BsmAI GTCTC 3 cut(s) 229, 274, 399
BsmBI CGTCTC 1 cut(s) 274
BsmFI GGGAC 2 cut(s) 244, 367
BsnI GGCC 3 cut(s) 106, 246, 336
BsoBI CYCGRG 1 cut(s) 194
Bsp1286I GDGCHC 4 cut(s) 30, 52, 327, 471
Bsp143I GATC 3 cut(s) 74, 418, 427
BspACI CCGC 3 cut(s) 151, 273, 398
BspANI GGCC 3 cut(s) 106, 246, 336
BspCNI CTCAG 3 cut(s) 16, 289, 457
BspLI GGNNCC 1 cut(s) 51
BspPI GGATC 2 cut(s) 413, 422
BspT107I GGYRCC 1 cut(s) 49
BsrBI CCGCTC 1 cut(s) 275
BsrI ACTGG 1 cut(s) 299
BssMI GATC 3 cut(s) 74, 418, 427
BssNI GRCGYC 2 cut(s) 267, 351
Bst2UI CCWGG 1 cut(s) 162
Bst6I CTCTTC 2 cut(s) 290, 392
BstACI GRCGYC 2 cut(s) 267, 351
BstC8I GCNNGC 1 cut(s) 186
BstDEI CTNAG 3 cut(s) 24, 276, 465
BstEII GGTNACC 1 cut(s) 94
BstKTI GATC 3 cut(s) 77, 421, 430
BstMAI GTCTC 3 cut(s) 229, 274, 399
BstMBI GATC 3 cut(s) 74, 418, 427
BstMCI CGRYCG 1 cut(s) 247
BstMWI GCNNNNNNNGC 2 cut(s) 185, 190
BstNI CCWGG 1 cut(s) 162
BstPI GGTNACC 1 cut(s) 94
BstSCI CCNGG 1 cut(s) 160
BstSLI GKGCMC 2 cut(s) 52, 327
BstZI CGGCCG 1 cut(s) 244
BsuI GTATCC 1 cut(s) 320
BsuRI GGCC 3 cut(s) 106, 246, 336
BtsI GCAGTG 1 cut(s) 408
BtsIMutI CAGTG 1 cut(s) 408
Cac8I GCNNGC 1 cut(s) 186
Cfr13I GGNCC 1 cut(s) 205
CseI GACGC 1 cut(s) 256
Csp6I GTAC 1 cut(s) 211
CviAII CATG 1 cut(s) 58
CviJI RGCY 9 cut(s) 35, 106, 179, 193, 246, 330, 336, 469, 475
CviKI_1 RGCY 9 cut(s) 35, 106, 179, 193, 246, 330, 336, 469, 475
CviQI GTAC 1 cut(s) 211
DdeI CTNAG 3 cut(s) 24, 276, 465
DpnI GATC 3 cut(s) 76, 420, 429
DpnII GATC 3 cut(s) 74, 418, 427
EaeI YGGCCR 1 cut(s) 244
EagI CGGCCG 1 cut(s) 244
Eam1104I CTCTTC 2 cut(s) 290, 392
EarI CTCTTC 2 cut(s) 290, 392
EciI GGCGGA 1 cut(s) 387
Ecl136II GAGCTC 1 cut(s) 469
EclXI CGGCCG 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 471
Eco47I GGWCC 1 cut(s) 205
Eco52I CGGCCG 1 cut(s) 244
Eco53kI GAGCTC 1 cut(s) 469
Eco88I CYCGRG 1 cut(s) 194
Eco91I GGTNACC 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 469
EcoO65I GGTNACC 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 160
EcoT38I GRGCYC 1 cut(s) 471
Esp3I CGTCTC 1 cut(s) 274
FaeI CATG 1 cut(s) 61
FaiI YATR 2 cut(s) 59, 91
FaqI GGGAC 2 cut(s) 244, 367
FatI CATG 1 cut(s) 57
Fnu4HI GCNGC 1 cut(s) 152
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 1 cut(s) 152
GluI GCNGC 1 cut(s) 152
GsaI CCCAGC 4 cut(s) 35, 57, 179, 330
HaeIII GGCC 3 cut(s) 106, 246, 336
HapII CCGG 4 cut(s) 83, 208, 264, 333
HgaI GACGC 1 cut(s) 256
Hin1I GRCGYC 2 cut(s) 267, 351
Hin1II CATG 1 cut(s) 61
HinfI GANTC 8 cut(s) 144, 199, 232, 249, 392, 485, 491, 497
HpaII CCGG 4 cut(s) 83, 208, 264, 333
HphI GGTGA 1 cut(s) 69
Hpy188I TCNGA 6 cut(s) 25, 314, 427, 466, 490, 496
Hpy188III TCNNGA 3 cut(s) 196, 278, 416
Hpy99I CGWCG 3 cut(s) 144, 353, 356
HpyAV CCTTC 2 cut(s) 432, 450
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 1 cut(s) 408
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 190
HpyF3I CTNAG 3 cut(s) 24, 276, 465
HpySE526I ACGT 1 cut(s) 351
Hsp92I GRCGYC 2 cut(s) 267, 351
Hsp92II CATG 1 cut(s) 61
Kzo9I GATC 3 cut(s) 74, 418, 427
LmnI GCTCC 1 cut(s) 335
LweI GCATC 2 cut(s) 76, 130
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 1 cut(s) 94
MalI GATC 3 cut(s) 76, 420, 429
MbiI CCGCTC 1 cut(s) 275
MboI GATC 3 cut(s) 74, 418, 427
MboII GAAGA 5 cut(s) 277, 379, 435, 438, 441
MhlI GDGCHC 4 cut(s) 30, 52, 327, 471
MluCI AATT 6 cut(s) 156, 454, 502, 508, 514, 521
MlyI GAGTC 2 cut(s) 226, 401
MseI TTAA 2 cut(s) 303, 544
MspA1I CMGCKG 1 cut(s) 151
MspI CCGG 4 cut(s) 83, 208, 264, 333
MspR9I CCNGG 1 cut(s) 162
MvaI CCWGG 1 cut(s) 162
MwoI GCNNNNNNNGC 2 cut(s) 185, 190
NdeII GATC 3 cut(s) 74, 418, 427
NlaIII CATG 1 cut(s) 61
NlaIV GGNNCC 1 cut(s) 51
PaeR7I CTCGAG 1 cut(s) 194
PcsI WCGNNNNNNNCGW 2 cut(s) 354, 360
PfeI GAWTC 6 cut(s) 144, 199, 249, 485, 491, 497
PkrI GCNGC 1 cut(s) 153
PleI GAGTC 2 cut(s) 226, 400
PpsI GAGTC 2 cut(s) 226, 400
Psp124BI GAGCTC 1 cut(s) 471
Psp6I CCWGG 1 cut(s) 160
PspEI GGTNACC 1 cut(s) 94
PspFI CCCAGC 4 cut(s) 31, 53, 175, 326
PspGI CCWGG 1 cut(s) 160
PspN4I GGNNCC 1 cut(s) 51
PspPI GGNCC 1 cut(s) 205
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
SacI GAGCTC 1 cut(s) 471
SaqAI TTAA 2 cut(s) 303, 544
SatI GCNGC 1 cut(s) 152
Sau3AI GATC 3 cut(s) 74, 418, 427
Sau96I GGNCC 1 cut(s) 205
SchI GAGTC 2 cut(s) 226, 401
ScrFI CCNGG 1 cut(s) 162
SduI GDGCHC 4 cut(s) 30, 52, 327, 471
SfaNI GCATC 2 cut(s) 76, 130
Sfr274I CTCGAG 1 cut(s) 194
SinI GGWCC 1 cut(s) 205
SlaI CTCGAG 1 cut(s) 194
SmlI CTYRAG 3 cut(s) 194, 470, 476
SmoI CTYRAG 3 cut(s) 194, 470, 476
Sse9I AATT 6 cut(s) 156, 454, 502, 508, 514, 521
SsiI CCGC 3 cut(s) 151, 273, 398
SspI AATATT 1 cut(s) 132
SstI GAGCTC 1 cut(s) 471
StyD4I CCNGG 1 cut(s) 160
TaiI ACGT 1 cut(s) 354
TaqI TCGA 7 cut(s) 139, 195, 348, 363, 390, 525, 539
TasI AATT 6 cut(s) 156, 454, 502, 508, 514, 521
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 6 cut(s) 144, 199, 249, 485, 491, 497
Tru1I TTAA 2 cut(s) 303, 544
Tru9I TTAA 2 cut(s) 303, 544
TscAI CASTG 1 cut(s) 415
TspRI CASTG 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 205
XapI RAATTY 1 cut(s) 156
XhoI CTCGAG 1 cut(s) 194
ZraI GACGTC 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.