RchiOBHm_Chr6g0278741

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
N/A
Physical Location & Seq
Forward (+)
41863642 .. 41865848
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 591 bp
ATGTTATCAGAGTTGATGAACTTCTTGAGGTCCTGTTTTCGGCCGAGGTCGAATGGATATGTTCATACCAGTTCAGATGCAGGGGGTCGCCAAGACGGGCTCCTGTGGTACAAAGACACAGGCCAGCACATCAATGGTGATTTCTCAATGGCTGTAGTTCAGGCCAACAATTTGCTTGAGGATCAGAGTCAGGTTGAGTCCGGGAGTTTGAGCACGAGTGAGTCTGGCCCTTTTGGAACTTTTATTGGTGTTTATGACGGGCATGGTGGACCGGAGACATCACGCTTTATCAATGATCACCTCTTTCAGCATCTCAAGAGATTTAGTTCGGAGCAGCAGTCCATGTCTGTGGATGTAATACGAAAAGCCTTTCAAGCAACCGAAGAGGGTTTTCTTTCTGTAGTTACCAGGCAGTGGCCTCTGAAACCACAAATTGCAGCAGTAGGATCCTGCTGCCTTGTTGGCGTTATTTGTAACGGAACTCTTTATATTGCCAACCTTGGTGATTCCCGTGCTGTTTTGGGGAGGATTGTGAAGGCAACAGGGAGGTACTCTCAATTCAGTTGTCACAAGAGCACAATGCATGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

196

Amino Acids

21.52

Weight (kDa)

7.04

Isoelectric Point (pI)

53.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 79 - 176 2.4e-13 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 414
AclWI GGATC 3 cut(s) 189, 441, 454
AcoI YGGCCR 1 cut(s) 41
AfaI GTAC 2 cut(s) 110, 551
AfiI CCNNNNNNNGG 2 cut(s) 39, 414
AgsI TTSAA 1 cut(s) 374
AjnI CCWGG 1 cut(s) 407
Alw21I GWGCWC 2 cut(s) 215, 578
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 3 cut(s) 189, 441, 454
AoxI GGCC 5 cut(s) 41, 121, 162, 226, 416
ApeKI GCWGC 3 cut(s) 334, 437, 453
AspS9I GGNCC 3 cut(s) 30, 227, 269
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 3 cut(s) 149, 290, 515
AvaII GGWCC 2 cut(s) 30, 269
BamHI GGATCC 1 cut(s) 446
BanII GRGCYC 1 cut(s) 102
BauI CACGAG 1 cut(s) 214
Bbv12I GWGCWC 2 cut(s) 215, 578
BbvI GCAGC 3 cut(s) 346, 440, 449
BciT130I CCWGG 1 cut(s) 409
BclI TGATCA 1 cut(s) 295
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 269
BfmI CTRYAG 2 cut(s) 153, 399
BglI GCCNNNNNGGC 1 cut(s) 462
BisI GCNGC 3 cut(s) 335, 438, 454
BlsI GCNGC 3 cut(s) 336, 439, 455
Bme1390I CCNGG 2 cut(s) 202, 409
Bme18I GGWCC 2 cut(s) 30, 269
BmgT120I GGNCC 3 cut(s) 30, 227, 269
BmiI GGNNCC 2 cut(s) 101, 448
BmrFI CCNGG 2 cut(s) 202, 409
BmsI GCATC 2 cut(s) 67, 319
BplI GAGNNNNNCTC 2 cut(s) 538, 570
BpuEI CTTGAG 3 cut(s) 46, 197, 299
BpuMI CCSGG 1 cut(s) 202
BsaJI CCNNGG 2 cut(s) 44, 499
BsaWI WCCGGW 1 cut(s) 271
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 414
Bse1I ACTGG 1 cut(s) 69
BseBI CCWGG 1 cut(s) 409
BseDI CCNNGG 2 cut(s) 44, 499
BseGI GGATG 1 cut(s) 358
BseLI CCNNNNNNNGG 2 cut(s) 39, 414
BseNI ACTGG 1 cut(s) 69
BseX3I CGGCCG 1 cut(s) 41
BseXI GCAGC 3 cut(s) 346, 440, 449
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 5 cut(s) 43, 123, 164, 228, 418
BsiEI CGRYCG 1 cut(s) 44
BsiHKAI GWGCWC 2 cut(s) 215, 578
BsiSI CCGG 2 cut(s) 201, 272
BslI CCNNNNNNNGG 2 cut(s) 39, 414
BsmAI GTCTC 1 cut(s) 269
BsnI GGCC 5 cut(s) 43, 123, 164, 228, 418
Bsp1286I GDGCHC 3 cut(s) 102, 215, 578
Bsp143I GATC 3 cut(s) 181, 295, 446
BspANI GGCC 5 cut(s) 43, 123, 164, 228, 418
BspLI GGNNCC 2 cut(s) 101, 448
BspPI GGATC 3 cut(s) 189, 441, 454
BsrI ACTGG 1 cut(s) 69
BssECI CCNNGG 2 cut(s) 44, 499
BssMI GATC 3 cut(s) 181, 295, 446
BssSI CACGAG 1 cut(s) 214
BssT1I CCWWGG 1 cut(s) 499
Bst2BI CACGAG 1 cut(s) 214
Bst2UI CCWGG 1 cut(s) 409
Bst6I CTCTTC 1 cut(s) 378
BstC8I GCNNGC 1 cut(s) 125
BstF5I GGATG 1 cut(s) 358
BstKTI GATC 3 cut(s) 184, 298, 449
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 3 cut(s) 181, 295, 446
BstMCI CGRYCG 1 cut(s) 44
BstMWI GCNNNNNNNGC 2 cut(s) 374, 462
BstNI CCWGG 1 cut(s) 409
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 2 cut(s) 200, 407
BstSFI CTRYAG 2 cut(s) 153, 399
BstV1I GCAGC 3 cut(s) 346, 440, 449
BstX2I RGATCY 1 cut(s) 446
BstXI CCANNNNNNTGG 1 cut(s) 349
BstYI RGATCY 1 cut(s) 446
BstZI CGGCCG 1 cut(s) 41
BsuRI GGCC 5 cut(s) 43, 123, 164, 228, 418
BtsCI GGATG 1 cut(s) 358
BtsI GCAGTG 1 cut(s) 419
BtsIMutI CAGTG 1 cut(s) 419
Cac8I GCNNGC 1 cut(s) 125
Cfr13I GGNCC 3 cut(s) 30, 227, 269
Csp6I GTAC 2 cut(s) 109, 550
CviAII CATG 3 cut(s) 263, 343, 584
CviJI RGCY 8 cut(s) 43, 100, 123, 152, 164, 228, 368, 418
CviKI_1 RGCY 8 cut(s) 43, 100, 123, 152, 164, 228, 368, 418
CviQI GTAC 2 cut(s) 109, 550
DpnI GATC 3 cut(s) 183, 297, 448
DpnII GATC 3 cut(s) 181, 295, 446
EaeI YGGCCR 1 cut(s) 41
EagI CGGCCG 1 cut(s) 41
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
EclXI CGGCCG 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 499
Eco24I GRGCYC 1 cut(s) 102
Eco47I GGWCC 2 cut(s) 30, 269
Eco52I CGGCCG 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 30
EcoRII CCWGG 1 cut(s) 407
EcoT14I CCWWGG 1 cut(s) 499
EcoT22I ATGCAT 1 cut(s) 585
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 499
FaeI CATG 3 cut(s) 266, 346, 587
FaiI YATR 8 cut(s) 60, 66, 255, 264, 344, 489, 585, 589
FatI CATG 3 cut(s) 262, 342, 583
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 3 cut(s) 335, 438, 454
FokI GGATG 1 cut(s) 365
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 3 cut(s) 335, 438, 454
GluI GCNGC 3 cut(s) 335, 438, 454
HaeIII GGCC 5 cut(s) 43, 123, 164, 228, 418
HapII CCGG 2 cut(s) 201, 272
Hin1II CATG 3 cut(s) 266, 346, 587
HinfI GANTC 4 cut(s) 187, 197, 221, 506
HpaII CCGG 2 cut(s) 201, 272
HphI GGTGA 3 cut(s) 149, 290, 515
Hpy166II GTNNAC 1 cut(s) 269
Hpy188I TCNGA 5 cut(s) 10, 76, 186, 331, 423
Hpy188III TCNNGA 2 cut(s) 25, 316
Hpy8I GTNNAC 1 cut(s) 269
HpyAV CCTTC 1 cut(s) 529
HpyCH4V TGCA 3 cut(s) 80, 437, 583
HpyF10VI GCNNNNNNNGC 2 cut(s) 374, 462
Hsp92II CATG 3 cut(s) 266, 346, 587
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 3 cut(s) 181, 295, 446
LmnI GCTCC 2 cut(s) 105, 331
Lsp1109I GCAGC 3 cut(s) 346, 440, 449
LweI GCATC 2 cut(s) 67, 319
MaeIII GTNAC 3 cut(s) 403, 473, 566
MalI GATC 3 cut(s) 183, 297, 448
MboI GATC 3 cut(s) 181, 295, 446
MboII GAAGA 1 cut(s) 395
MflI RGATCY 1 cut(s) 446
MhlI GDGCHC 3 cut(s) 102, 215, 578
MluCI AATT 3 cut(s) 169, 432, 557
MlyI GAGTC 3 cut(s) 196, 206, 230
MnlI CCTC 8 cut(s) 21, 39, 172, 311, 379, 429, 519, 540
Mph1103I ATGCAT 1 cut(s) 585
MslI CAYNNNNRTG 2 cut(s) 132, 347
MspI CCGG 2 cut(s) 201, 272
MspR9I CCNGG 2 cut(s) 202, 409
MvaI CCWGG 1 cut(s) 409
MwoI GCNNNNNNNGC 2 cut(s) 374, 462
NciI CCSGG 1 cut(s) 202
NdeII GATC 3 cut(s) 181, 295, 446
NlaIII CATG 3 cut(s) 266, 346, 587
NlaIV GGNNCC 2 cut(s) 101, 448
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 566
NsiI ATGCAT 1 cut(s) 585
NspI RCATGY 1 cut(s) 587
PfeI GAWTC 1 cut(s) 506
PflMI CCANNNNNTGG 1 cut(s) 414
PfoI TCCNGGA 1 cut(s) 200
PkrI GCNGC 3 cut(s) 336, 439, 455
PleI GAGTC 3 cut(s) 195, 205, 229
PpsI GAGTC 3 cut(s) 195, 205, 229
PpuMI RGGWCCY 1 cut(s) 30
Psp5II RGGWCCY 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 407
PspGI CCWGG 1 cut(s) 407
PspN4I GGNNCC 2 cut(s) 101, 448
PspPI GGNCC 3 cut(s) 30, 227, 269
PspPPI RGGWCCY 1 cut(s) 30
PsuI RGATCY 1 cut(s) 446
RsaI GTAC 2 cut(s) 110, 551
RsaNI GTAC 2 cut(s) 109, 550
RseI CAYNNNNRTG 2 cut(s) 132, 347
SatI GCNGC 3 cut(s) 335, 438, 454
Sau3AI GATC 3 cut(s) 181, 295, 446
Sau96I GGNCC 3 cut(s) 30, 227, 269
SchI GAGTC 3 cut(s) 196, 206, 230
ScrFI CCNGG 2 cut(s) 202, 409
SduI GDGCHC 3 cut(s) 102, 215, 578
SetI ASST 6 cut(s) 32, 50, 195, 303, 501, 551
SfaNI GCATC 2 cut(s) 67, 319
SfcI CTRYAG 2 cut(s) 153, 399
SinI GGWCC 2 cut(s) 30, 269
SmiMI CAYNNNNRTG 2 cut(s) 132, 347
SmlI CTYRAG 3 cut(s) 25, 176, 314
SmoI CTYRAG 3 cut(s) 25, 176, 314
Sse9I AATT 3 cut(s) 169, 432, 557
StyD4I CCNGG 2 cut(s) 200, 407
StyI CCWWGG 1 cut(s) 499
TaqI TCGA 1 cut(s) 50
TasI AATT 3 cut(s) 169, 432, 557
TfiI GAWTC 1 cut(s) 506
TscAI CASTG 1 cut(s) 419
TseFI GTSAC 1 cut(s) 566
TseI GCWGC 3 cut(s) 334, 437, 453
Tsp45I GTSAC 1 cut(s) 566
TspDTI ATGAA 2 cut(s) 32, 53
TspGWI ACGGA 1 cut(s) 492
TspRI CASTG 1 cut(s) 419
Van91I CCANNNNNTGG 1 cut(s) 414
VpaK11BI GGWCC 2 cut(s) 30, 269
XceI RCATGY 1 cut(s) 587
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
Zsp2I ATGCAT 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.