Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1 NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1
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No orthologs found for this gene in the current database.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 183 |
| AgsI | TTSAA | 2 cut(s) | 94, 151 |
| AjnI | CCWGG | 1 cut(s) | 132 |
| AluBI | AGCT | 2 cut(s) | 10, 169 |
| AluI | AGCT | 2 cut(s) | 10, 169 |
| Alw26I | GTCTC | 1 cut(s) | 207 |
| AoxI | GGCC | 1 cut(s) | 64 |
| ApoI | RAATTY | 1 cut(s) | 183 |
| AsuHPI | GGTGA | 1 cut(s) | 16 |
| BciT130I | CCWGG | 1 cut(s) | 134 |
| BcoDI | GTCTC | 1 cut(s) | 207 |
| Bme1390I | CCNGG | 1 cut(s) | 134 |
| BmrFI | CCNGG | 1 cut(s) | 134 |
| BmrI | ACTGGG | 1 cut(s) | 81 |
| BmuI | ACTGGG | 1 cut(s) | 81 |
| BpuEI | CTTGAG | 1 cut(s) | 185 |
| BsaJI | CCNNGG | 1 cut(s) | 158 |
| Bse1I | ACTGG | 1 cut(s) | 76 |
| BseBI | CCWGG | 1 cut(s) | 134 |
| BseDI | CCNNGG | 1 cut(s) | 158 |
| BseGI | GGATG | 1 cut(s) | 142 |
| BseNI | ACTGG | 1 cut(s) | 76 |
| BshFI | GGCC | 1 cut(s) | 66 |
| BsmAI | GTCTC | 1 cut(s) | 207 |
| BsnI | GGCC | 1 cut(s) | 66 |
| Bsp143I | GATC | 1 cut(s) | 45 |
| Bsp19I | CCATGG | 1 cut(s) | 158 |
| BspANI | GGCC | 1 cut(s) | 66 |
| BsrI | ACTGG | 1 cut(s) | 76 |
| BssECI | CCNNGG | 1 cut(s) | 158 |
| BssMI | GATC | 1 cut(s) | 45 |
| BssT1I | CCWWGG | 1 cut(s) | 158 |
| Bst2UI | CCWGG | 1 cut(s) | 134 |
| Bst4CI | ACNGT | 1 cut(s) | 106 |
| BstC8I | GCNNGC | 2 cut(s) | 30, 55 |
| BstDEI | CTNAG | 1 cut(s) | 170 |
| BstDSI | CCRYGG | 1 cut(s) | 158 |
| BstF5I | GGATG | 1 cut(s) | 142 |
| BstKTI | GATC | 1 cut(s) | 48 |
| BstMAI | GTCTC | 1 cut(s) | 207 |
| BstMBI | GATC | 1 cut(s) | 45 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 63 |
| BstNI | CCWGG | 1 cut(s) | 134 |
| BstNSI | RCATGY | 1 cut(s) | 32 |
| BstSCI | CCNGG | 1 cut(s) | 132 |
| BsuRI | GGCC | 1 cut(s) | 66 |
| BtgI | CCRYGG | 1 cut(s) | 158 |
| BtsCI | GGATG | 1 cut(s) | 142 |
| BtsIMutI | CAGTG | 1 cut(s) | 69 |
| Cac8I | GCNNGC | 2 cut(s) | 30, 55 |
| CviAII | CATG | 3 cut(s) | 29, 159, 195 |
| CviJI | RGCY | 6 cut(s) | 10, 66, 76, 121, 163, 169 |
| CviKI_1 | RGCY | 6 cut(s) | 10, 66, 76, 121, 163, 169 |
| DdeI | CTNAG | 1 cut(s) | 170 |
| DpnI | GATC | 1 cut(s) | 47 |
| DpnII | GATC | 1 cut(s) | 45 |
| Eco130I | CCWWGG | 1 cut(s) | 158 |
| EcoRI | GAATTC | 1 cut(s) | 183 |
| EcoRII | CCWGG | 1 cut(s) | 132 |
| EcoT14I | CCWWGG | 1 cut(s) | 158 |
| ErhI | CCWWGG | 1 cut(s) | 158 |
| FaeI | CATG | 3 cut(s) | 32, 162, 198 |
| FaiI | YATR | 7 cut(s) | 22, 30, 63, 102, 160, 180, 196 |
| FatI | CATG | 3 cut(s) | 28, 158, 194 |
| FokI | GGATG | 1 cut(s) | 149 |
| HaeIII | GGCC | 1 cut(s) | 66 |
| Hin1II | CATG | 3 cut(s) | 32, 162, 198 |
| HinfI | GANTC | 1 cut(s) | 113 |
| HphI | GGTGA | 1 cut(s) | 16 |
| Hpy188I | TCNGA | 1 cut(s) | 218 |
| Hpy188III | TCNNGA | 1 cut(s) | 13 |
| HpyAV | CCTTC | 2 cut(s) | 88, 152 |
| HpyCH4III | ACNGT | 1 cut(s) | 106 |
| HpyCH4V | TGCA | 1 cut(s) | 32 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 63 |
| HpyF3I | CTNAG | 1 cut(s) | 170 |
| Hsp92II | CATG | 3 cut(s) | 32, 162, 198 |
| Kzo9I | GATC | 1 cut(s) | 45 |
| LpnPI | CCDG | 3 cut(s) | 57, 119, 146 |
| MalI | GATC | 1 cut(s) | 47 |
| MboI | GATC | 1 cut(s) | 45 |
| MluCI | AATT | 1 cut(s) | 183 |
| MlyI | GAGTC | 1 cut(s) | 122 |
| MnlI | CCTC | 2 cut(s) | 77, 167 |
| MslI | CAYNNNNRTG | 1 cut(s) | 33 |
| MspR9I | CCNGG | 1 cut(s) | 134 |
| MvaI | CCWGG | 1 cut(s) | 134 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 63 |
| NcoI | CCATGG | 1 cut(s) | 158 |
| NdeII | GATC | 1 cut(s) | 45 |
| NlaIII | CATG | 3 cut(s) | 32, 162, 198 |
| NspI | RCATGY | 1 cut(s) | 32 |
| PaeI | GCATGC | 1 cut(s) | 32 |
| PfoI | TCCNGGA | 1 cut(s) | 132 |
| PleI | GAGTC | 1 cut(s) | 121 |
| PpsI | GAGTC | 1 cut(s) | 121 |
| Psp6I | CCWGG | 1 cut(s) | 132 |
| PspGI | CCWGG | 1 cut(s) | 132 |
| RseI | CAYNNNNRTG | 1 cut(s) | 33 |
| Sau3AI | GATC | 1 cut(s) | 45 |
| SchI | GAGTC | 1 cut(s) | 122 |
| ScrFI | CCNGG | 1 cut(s) | 134 |
| SetI | ASST | 3 cut(s) | 12, 144, 171 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 33 |
| SmlI | CTYRAG | 2 cut(s) | 11, 164 |
| SmoI | CTYRAG | 2 cut(s) | 11, 164 |
| SphI | GCATGC | 1 cut(s) | 32 |
| Sse9I | AATT | 1 cut(s) | 183 |
| StyD4I | CCNGG | 1 cut(s) | 132 |
| StyI | CCWWGG | 1 cut(s) | 158 |
| TaaI | ACNGT | 1 cut(s) | 106 |
| TasI | AATT | 1 cut(s) | 183 |
| TscAI | CASTG | 1 cut(s) | 76 |
| TspDTI | ATGAA | 1 cut(s) | 183 |
| TspGWI | ACGGA | 1 cut(s) | 71 |
| TspRI | CASTG | 1 cut(s) | 76 |
| XapI | RAATTY | 1 cut(s) | 183 |
| XceI | RCATGY | 1 cut(s) | 32 |