RchiOBHm_Chr6g0288741

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
N/A
Physical Location & Seq
Reverse (-)
51889260 .. 51895112
5853 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1101 bp
ATGGAAGCTGTAGATCATAGTGAGGCTGAGAAGACGGTGAAAGAATTTGACGAAACCAAAGCTGGTGTCAAAGGAATTGTTGACTCTGGAGTGACCAAGATCCCAAGAATGTTCATACACCCTCTAGAGGATTTGCAACAAAACAACATGGATCATGATCTCCGAGTTCCAATGATTGATCTCAAAGGCCTTGATCAGAGCCCAGAGAGGCGAAAAGAAGTCATCCATCAGATAAGCAAAGCAGCTGAAGCGTGGGGCTTCTTTCAGATAGTGAACCATGGAGTTCCACTTGATGTTATGGAGGAGGTGCTGAAAAGTGTTCGACGCTTTCACGAGCAGCCCCATGAAGCCAAGGTGGAATGGTACTCACGCGATTTTACAAAAAAGGTAAACTACTTCAGCAATGGCGATTTGAAGGCATCCACTCCTGCGGATTGGAGGGACACGTTATCCTGCAACTGCCAGTTCCTAGAAGATGAAAGCAACTTCCAGGCGCTGCCTCGAGTATGCAGTGACTACCTTTCAACTCTAGGGTGCTTCAAATCATCAACACTATCGTGCCATTATTACCCAGTTTGTCCCGAGCCCCATTTGACTTTAGGTGCAATCAAGCACTCTGACTTCCCCTTTCTCACTTTGCTGCTTCAGGACAGTAGTGGTGGCTTCCAAGTGCTTCATCAAAATGTTTGGGTTAATGTTCCTCCACTGCAGGGAGTTTTGGTAGCAAATATTGGCGACATGATGCAGATCACTAGCAATGACAAGTTTTGTTGCCCAATTACTAGAAGGTCTACTACATGCTATTATGTTTTTCTTGGACCCTCTTTGGTTTCATGGAGATCAAAACGGCAGAAAACAGTTTCTCTCTCTTCAGCAGAGGCAGAGTATAGAGCAATGACGGGGGCTTGCTGTGAATTAACCTGGTTGGAATTTGTTCTCCGGGATTTGGGTGTATTACAGCCAAAACCTGCACTACTATATTGTGATAATAAAGCAGCGCTGCATATTGCTGCTAACCCTGTTTTCCATGAAAGAACAAGGCACATTGAAATGGATTGTCACTTCATCAGAGATAAAATTCAAGACGGTCAAAGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

366

Amino Acids

41.64

Weight (kDa)

6.01

Isoelectric Point (pI)

47.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 56 - 161 1.5e-23 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 183 - 257 1.7e-20 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 976
AccI GTMKAC 1 cut(s) 791
AccII CGCG 1 cut(s) 372
AciI CCGC 1 cut(s) 431
AclWI GGATC 2 cut(s) 94, 159
AcsI RAATTY 3 cut(s) 44, 929, 1077
AcuI CTGAAG 4 cut(s) 267, 382, 629, 855
AfaI GTAC 1 cut(s) 365
AfeI AGCGCT 1 cut(s) 999
AfiI CCNNNNNNNGG 3 cut(s) 127, 710, 946
AflIII ACRYGT 1 cut(s) 444
AgsI TTSAA 5 cut(s) 415, 525, 541, 1049, 1082
AjnI CCWGG 2 cut(s) 489, 920
AleI CACNNNNGTG 1 cut(s) 556
AluBI AGCT 3 cut(s) 8, 62, 245
AluI AGCT 3 cut(s) 8, 62, 245
AlwI GGATC 2 cut(s) 94, 159
AlwNI CAGNNNCTG 1 cut(s) 496
Ama87I CYCGRG 2 cut(s) 501, 581
Aor51HI AGCGCT 1 cut(s) 999
AoxI GGCC 1 cut(s) 187
ApeKI GCWGC 7 cut(s) 242, 337, 496, 640, 995, 1000, 1010
ApoI RAATTY 3 cut(s) 44, 929, 1077
ArsI GACNNNNNNTTYG 2 cut(s) 51, 83
Asp700I GAANNNNTTC 1 cut(s) 933
AspLEI GCGC 2 cut(s) 496, 1000
AspS9I GGNCC 1 cut(s) 818
AsuC2I CCSGG 1 cut(s) 941
AsuHPI GGTGA 1 cut(s) 49
AvaI CYCGRG 2 cut(s) 501, 581
AvaII GGWCC 1 cut(s) 818
BanII GRGCYC 2 cut(s) 203, 588
BarI GAAGNNNNNNTAC 2 cut(s) 778, 810
BauI CACGAG 1 cut(s) 332
BbsI GAAGAC 1 cut(s) 38
BbvI GCAGC 7 cut(s) 254, 349, 483, 627, 987, 997, 1007
BccI CCATC 1 cut(s) 234
BceAI ACGGC 1 cut(s) 863
BciT130I CCWGG 2 cut(s) 491, 922
BclI TGATCA 1 cut(s) 193
BcnI CCSGG 1 cut(s) 941
BfaI CTAG 5 cut(s) 125, 470, 530, 753, 783
BfmI CTRYAG 2 cut(s) 9, 707
BfoI RGCGCY 2 cut(s) 497, 1001
BfuAI ACCTGC 1 cut(s) 976
BisI GCNGC 7 cut(s) 243, 338, 497, 641, 996, 1001, 1011
BlsI GCNGC 7 cut(s) 244, 339, 498, 642, 997, 1002, 1012
Bme1390I CCNGG 3 cut(s) 491, 922, 941
Bme18I GGWCC 1 cut(s) 818
BmeT110I CYCGRG 2 cut(s) 501, 581
BmgT120I GGNCC 1 cut(s) 818
BmiI GGNNCC 1 cut(s) 820
BmrFI CCNGG 3 cut(s) 491, 922, 941
BmrI ACTGGG 1 cut(s) 566
BmsI GCATC 2 cut(s) 428, 732
BmuI ACTGGG 1 cut(s) 566
BpiI GAAGAC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 108
BpuMI CCSGG 1 cut(s) 941
BsaBI GATNNNNATC 2 cut(s) 156, 746
BsaJI CCNNGG 2 cut(s) 277, 351
BsaXI ACNNNNNCTCC 2 cut(s) 705, 735
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 710, 946
Bse1I ACTGG 2 cut(s) 463, 572
Bse3DI GCAATG 3 cut(s) 409, 763, 900
Bse8I GATNNNNATC 2 cut(s) 156, 746
BseBI CCWGG 2 cut(s) 491, 922
BseDI CCNNGG 2 cut(s) 277, 351
BseGI GGATG 2 cut(s) 222, 419
BseJI GATNNNNATC 2 cut(s) 156, 746
BseLI CCNNNNNNNGG 3 cut(s) 127, 710, 946
BseMI GCAATG 3 cut(s) 409, 763, 900
BseMII CTCAG 1 cut(s) 18
BseNI ACTGG 2 cut(s) 463, 572
BseRI GAGGAG 1 cut(s) 317
BseXI GCAGC 7 cut(s) 254, 349, 483, 627, 987, 997, 1007
BsgI GTGCAG 1 cut(s) 954
Bsh1236I CGCG 1 cut(s) 372
BshFI GGCC 1 cut(s) 189
BsiHKCI CYCGRG 2 cut(s) 501, 581
BsiSI CCGG 1 cut(s) 940
BslFI GGGAC 2 cut(s) 455, 564
BslI CCNNNNNNNGG 3 cut(s) 127, 710, 946
BsmFI GGGAC 2 cut(s) 455, 564
BsnI GGCC 1 cut(s) 189
BsoBI CYCGRG 2 cut(s) 501, 581
Bsp1286I GDGCHC 2 cut(s) 203, 588
Bsp143I GATC 8 cut(s) 13, 99, 151, 157, 178, 193, 747, 839
Bsp19I CCATGG 1 cut(s) 277
BspACI CCGC 1 cut(s) 431
BspANI GGCC 1 cut(s) 189
BspCNI CTCAG 1 cut(s) 19
BspFNI CGCG 1 cut(s) 372
BspHI TCATGA 1 cut(s) 154
BspLI GGNNCC 1 cut(s) 820
BspMAI CTGCAG 1 cut(s) 711
BspMI ACCTGC 1 cut(s) 976
BspPI GGATC 2 cut(s) 94, 159
BsrDI GCAATG 3 cut(s) 409, 763, 900
BsrI ACTGG 2 cut(s) 463, 572
BssECI CCNNGG 2 cut(s) 277, 351
BssMI GATC 8 cut(s) 13, 99, 151, 157, 178, 193, 747, 839
BssSI CACGAG 1 cut(s) 332
BssT1I CCWWGG 2 cut(s) 277, 351
Bst2BI CACGAG 1 cut(s) 332
Bst2UI CCWGG 2 cut(s) 491, 922
Bst4CI ACNGT 4 cut(s) 37, 653, 859, 1088
Bst6I CTCTTC 1 cut(s) 874
BstC8I GCNNGC 1 cut(s) 907
BstDEI CTNAG 1 cut(s) 27
BstDSI CCRYGG 1 cut(s) 277
BstF5I GGATG 2 cut(s) 222, 419
BstFNI CGCG 1 cut(s) 372
BstH2I RGCGCY 2 cut(s) 497, 1001
BstHHI GCGC 2 cut(s) 496, 1000
BstKTI GATC 8 cut(s) 16, 102, 154, 160, 181, 196, 750, 842
BstMBI GATC 8 cut(s) 13, 99, 151, 157, 178, 193, 747, 839
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstNI CCWGG 2 cut(s) 491, 922
BstNSI RCATGY 1 cut(s) 801
BstSCI CCNGG 3 cut(s) 489, 920, 939
BstSFI CTRYAG 2 cut(s) 9, 707
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 7 cut(s) 254, 349, 483, 627, 987, 997, 1007
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 1 cut(s) 99
BstYI RGATCY 1 cut(s) 99
BsuRI GGCC 1 cut(s) 189
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 2 cut(s) 222, 419
BtsI GCAGTG 2 cut(s) 517, 704
BtsIMutI CAGTG 2 cut(s) 517, 704
BveI ACCTGC 1 cut(s) 976
Cac8I GCNNGC 1 cut(s) 907
CaiI CAGNNNCTG 1 cut(s) 496
CciI TCATGA 1 cut(s) 154
CfoI GCGC 2 cut(s) 496, 1000
Cfr13I GGNCC 1 cut(s) 818
CseI GACGC 1 cut(s) 333
CsiI ACCWGGT 1 cut(s) 920
Csp6I GTAC 1 cut(s) 364
CviAII CATG 8 cut(s) 148, 155, 278, 344, 739, 798, 834, 1028
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 1 cut(s) 27
DpnI GATC 8 cut(s) 15, 101, 153, 159, 180, 195, 749, 841
DpnII GATC 8 cut(s) 13, 99, 151, 157, 178, 193, 747, 839
Eam1104I CTCTTC 1 cut(s) 874
EarI CTCTTC 1 cut(s) 874
Eco130I CCWWGG 2 cut(s) 277, 351
Eco147I AGGCCT 1 cut(s) 189
Eco24I GRGCYC 2 cut(s) 203, 588
Eco47I GGWCC 1 cut(s) 818
Eco47III AGCGCT 1 cut(s) 999
Eco57I CTGAAG 4 cut(s) 267, 382, 629, 855
Eco88I CYCGRG 2 cut(s) 501, 581
EcoRII CCWGG 2 cut(s) 489, 920
EcoT14I CCWWGG 2 cut(s) 277, 351
EcoT38I GRGCYC 2 cut(s) 203, 588
ErhI CCWWGG 2 cut(s) 277, 351
FaeI CATG 8 cut(s) 151, 158, 281, 347, 742, 801, 837, 1031
FaqI GGGAC 2 cut(s) 455, 564
FatI CATG 8 cut(s) 147, 154, 277, 343, 738, 797, 833, 1027
FbaI TGATCA 1 cut(s) 193
FblI GTMKAC 1 cut(s) 791
Fnu4HI GCNGC 7 cut(s) 243, 338, 497, 641, 996, 1001, 1011
FokI GGATG 2 cut(s) 209, 406
FriOI GRGCYC 2 cut(s) 203, 588
Fsp4HI GCNGC 7 cut(s) 243, 338, 497, 641, 996, 1001, 1011
FspBI CTAG 5 cut(s) 125, 470, 530, 753, 783
GlaI GCGC 2 cut(s) 495, 999
GluI GCNGC 7 cut(s) 243, 338, 497, 641, 996, 1001, 1011
GsuI CTGGAG 1 cut(s) 108
HaeII RGCGCY 2 cut(s) 497, 1001
HaeIII GGCC 1 cut(s) 189
HapII CCGG 1 cut(s) 940
HgaI GACGC 1 cut(s) 333
HhaI GCGC 2 cut(s) 496, 1000
Hin1II CATG 8 cut(s) 151, 158, 281, 347, 742, 801, 837, 1031
Hin6I GCGC 2 cut(s) 494, 998
HinP1I GCGC 2 cut(s) 494, 998
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HinfI GANTC 1 cut(s) 83
HpaII CCGG 1 cut(s) 940
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 4 cut(s) 82, 274, 391, 792
Hpy188I TCNGA 6 cut(s) 164, 198, 231, 267, 619, 1070
Hpy188III TCNNGA 7 cut(s) 87, 125, 155, 332, 581, 647, 1082
Hpy8I GTNNAC 4 cut(s) 82, 274, 391, 792
Hpy99I CGWCG 1 cut(s) 327
HpyAV CCTTC 2 cut(s) 409, 780
HpyCH4III ACNGT 4 cut(s) 37, 653, 859, 1088
HpyCH4IV ACGT 1 cut(s) 446
HpyCH4V TGCA 8 cut(s) 136, 456, 510, 605, 709, 745, 971, 1003
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 1 cut(s) 27
HpySE526I ACGT 1 cut(s) 446
Hsp92II CATG 8 cut(s) 151, 158, 281, 347, 742, 801, 837, 1031
HspAI GCGC 2 cut(s) 494, 998
Ksp22I TGATCA 1 cut(s) 193
Kzo9I GATC 8 cut(s) 13, 99, 151, 157, 178, 193, 747, 839
Lsp1109I GCAGC 7 cut(s) 254, 349, 483, 627, 987, 997, 1007
LweI GCATC 2 cut(s) 428, 732
MabI ACCWGGT 1 cut(s) 920
MaeI CTAG 5 cut(s) 125, 470, 530, 753, 783
MaeII ACGT 1 cut(s) 446
MaeIII GTNAC 3 cut(s) 91, 512, 1058
MalI GATC 8 cut(s) 15, 101, 153, 159, 180, 195, 749, 841
MboI GATC 8 cut(s) 13, 99, 151, 157, 178, 193, 747, 839
MboII GAAGA 3 cut(s) 43, 485, 861
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 2 cut(s) 203, 588
MluCI AATT 6 cut(s) 44, 75, 777, 914, 929, 1077
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 906
MroXI GAANNNNTTC 1 cut(s) 933
MseI TTAA 2 cut(s) 693, 917
MslI CAYNNNNRTG 3 cut(s) 556, 681, 1049
MspA1I CMGCKG 1 cut(s) 245
MspI CCGG 1 cut(s) 940
MspR9I CCNGG 3 cut(s) 491, 922, 941
MvaI CCWGG 2 cut(s) 491, 922
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 1 cut(s) 248
NciI CCSGG 1 cut(s) 941
NcoI CCATGG 1 cut(s) 277
NdeII GATC 8 cut(s) 13, 99, 151, 157, 178, 193, 747, 839
NlaIII CATG 8 cut(s) 151, 158, 281, 347, 742, 801, 837, 1031
NlaIV GGNNCC 1 cut(s) 820
NmuCI GTSAC 3 cut(s) 91, 512, 1058
NspI RCATGY 1 cut(s) 801
OliI CACNNNNGTG 1 cut(s) 556
PaeR7I CTCGAG 1 cut(s) 501
PagI TCATGA 1 cut(s) 154
PceI AGGCCT 1 cut(s) 189
PdmI GAANNNNTTC 1 cut(s) 933
PfoI TCCNGGA 1 cut(s) 939
PkrI GCNGC 7 cut(s) 244, 339, 498, 642, 997, 1002, 1012
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Psp6I CCWGG 2 cut(s) 489, 920
PspGI CCWGG 2 cut(s) 489, 920
PspN4I GGNNCC 1 cut(s) 820
PspPI GGNCC 1 cut(s) 818
PspXI VCTCGAGB 1 cut(s) 501
PstI CTGCAG 1 cut(s) 711
PstNI CAGNNNCTG 1 cut(s) 496
PsuI RGATCY 1 cut(s) 99
PvuII CAGCTG 1 cut(s) 245
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 3 cut(s) 556, 681, 1049
SaqAI TTAA 2 cut(s) 693, 917
SatI GCNGC 7 cut(s) 243, 338, 497, 641, 996, 1001, 1011
Sau3AI GATC 8 cut(s) 13, 99, 151, 157, 178, 193, 747, 839
Sau96I GGNCC 1 cut(s) 818
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 3 cut(s) 491, 922, 941
SduI GDGCHC 2 cut(s) 203, 588
SexAI ACCWGGT 1 cut(s) 920
SfaNI GCATC 2 cut(s) 428, 732
SfcI CTRYAG 2 cut(s) 9, 707
Sfr274I CTCGAG 1 cut(s) 501
SinI GGWCC 1 cut(s) 818
SlaI CTCGAG 1 cut(s) 501
SmiMI CAYNNNNRTG 3 cut(s) 556, 681, 1049
SmlI CTYRAG 1 cut(s) 501
SmoI CTYRAG 1 cut(s) 501
Sse9I AATT 6 cut(s) 44, 75, 777, 914, 929, 1077
SseBI AGGCCT 1 cut(s) 189
SsiI CCGC 1 cut(s) 431
SspI AATATT 1 cut(s) 730
SspMI CTAG 5 cut(s) 125, 470, 530, 753, 783
StuI AGGCCT 1 cut(s) 189
StyD4I CCNGG 3 cut(s) 489, 920, 939
StyI CCWWGG 2 cut(s) 277, 351
TaaI ACNGT 4 cut(s) 37, 653, 859, 1088
TaiI ACGT 1 cut(s) 449
TaqI TCGA 2 cut(s) 322, 502
TasI AATT 6 cut(s) 44, 75, 777, 914, 929, 1077
Tru1I TTAA 2 cut(s) 693, 917
Tru9I TTAA 2 cut(s) 693, 917
TscAI CASTG 2 cut(s) 517, 711
TseFI GTSAC 3 cut(s) 91, 512, 1058
TseI GCWGC 7 cut(s) 242, 337, 496, 640, 995, 1000, 1010
Tsp45I GTSAC 3 cut(s) 91, 512, 1058
TspDTI ATGAA 7 cut(s) 103, 360, 492, 665, 822, 1044, 1054
TspRI CASTG 2 cut(s) 517, 711
VpaK11BI GGWCC 1 cut(s) 818
XapI RAATTY 3 cut(s) 44, 929, 1077
XbaI TCTAGA 1 cut(s) 124
XceI RCATGY 1 cut(s) 801
XhoI CTCGAG 1 cut(s) 501
XmiI GTMKAC 1 cut(s) 791
XmnI GAANNNNTTC 1 cut(s) 933
XspI CTAG 5 cut(s) 125, 470, 530, 753, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.