RchiOBHm_Chr6g0308071

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0030391
Physical Location & Seq
Reverse (-)
66198930 .. 66200796
1867 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 888 bp
ATGTCTAAACCATTCAACTTAGTTTTAAACATTGGTGTGCAGGAGAGGTGGTCGAGCCGGATTCATTATTATCGACATCAGTGTCGGGGAATCTTGACAGGAGATCGAATTGAATTAGACAAGAGCATCAAACCTGAGGATACAAGATACAAAGCGGCTCTCTCCATCATGGCTGCTAAAAGTTGTCATACGAGAACAAAGATCTCGTTCAATCCGTCGTTAATCACCACTGGAAGAGGCACTGCTCCATTTGATGCGGATGCATGGAGGACAGATTTCGACATCTCATGGTGCGAGTTCAACAAAGTCGGTAAGACGCACAGCGGCTTCATGAAAGCTCTCGGCCTACAACCAAACAATCGTTGGCCACTTGAACTAGAAACTGATCAACTACAAGGCAGTCACCAACATCCTCAGTTTGCTTACTACACAATCAGACAAATGCTGAAAGATTTACTGCAAGAGGATGAGAAAGCCAAGTTTATATTGACGGGGCACAGCTTGGGCGGGGCGTTGGCGATTTTGTTTGCCAGGGTGCTGGACTGCTGGCAATGCATGACTGTGACCCAGGTTGGAGATGGGAAGTTTGGGGTGTTTATGAAGGAAAAGTTTAGGAAATATGATGTGAGATATATGAGGTATGTTTACAGCAATGACCTGGTGCCTAGAATACCTTATGATGATAAAGCCTTCTTGTTCAAGCACTTTGGGCCAAGTCTTTACTACAACAGCTGCTATGGAGTAAAGGTTTTGAAGGAGGAACCCAACAAGAACTACCTTAGTTTGCTATGGTTCATACCTAAGAGGCTGAATGCAGTGTGGGAGCTGTTAAGGAGCTTCATACTCCCTTTGGTAAGGGGCTCAGACTACAAAGAGGGGTGGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

295

Amino Acids

34.41

Weight (kDa)

9.34

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 79 - 227 8.8e-20 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030391)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0308071
rosa_samantha Rh6AG472500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 81
AccB1I GGYRCC 1 cut(s) 661
AciI CCGC 4 cut(s) 155, 257, 324, 507
AcoI YGGCCR 1 cut(s) 365
AgsI TTSAA 7 cut(s) 16, 113, 211, 301, 374, 700, 754
AjnI CCWGG 3 cut(s) 530, 567, 657
AluBI AGCT 5 cut(s) 338, 501, 732, 826, 837
AluI AGCT 5 cut(s) 338, 501, 732, 826, 837
AoxI GGCC 3 cut(s) 343, 365, 710
ApeKI GCWGC 2 cut(s) 173, 732
AspS9I GGNCC 1 cut(s) 710
AsuHPI GGTGA 2 cut(s) 217, 395
AxyI CCTNAGG 1 cut(s) 135
BaeGI GKGCMC 1 cut(s) 498
BalI TGGCCA 1 cut(s) 367
BanI GGYRCC 1 cut(s) 661
BanII GRGCYC 1 cut(s) 863
BbvI GCAGC 2 cut(s) 160, 719
BccI CCATC 2 cut(s) 173, 572
BciT130I CCWGG 3 cut(s) 532, 569, 659
BciVI GTATCC 1 cut(s) 133
BclI TGATCA 1 cut(s) 385
BfaI CTAG 2 cut(s) 377, 666
BfuI GTATCC 1 cut(s) 133
BglII AGATCT 1 cut(s) 201
BisI GCNGC 4 cut(s) 156, 174, 325, 733
BlsI GCNGC 4 cut(s) 157, 175, 326, 734
Bme1390I CCNGG 3 cut(s) 532, 569, 659
BmgT120I GGNCC 1 cut(s) 710
BmiI GGNNCC 2 cut(s) 663, 762
BmrFI CCNGG 3 cut(s) 532, 569, 659
BmsI GCATC 3 cut(s) 135, 244, 250
BsaJI CCNNGG 2 cut(s) 531, 567
BsaXI ACNNNNNCTCC 4 cut(s) 35, 65, 732, 762
Bse1I ACTGG 1 cut(s) 235
Bse21I CCTNAGG 1 cut(s) 135
Bse3DI GCAATG 2 cut(s) 557, 658
BseBI CCWGG 3 cut(s) 532, 569, 659
BseDI CCNNGG 2 cut(s) 531, 567
BseGI GGATG 3 cut(s) 265, 409, 472
BseMI GCAATG 2 cut(s) 557, 658
BseMII CTCAG 3 cut(s) 126, 428, 876
BseNI ACTGG 1 cut(s) 235
BseSI GKGCMC 1 cut(s) 498
BseXI GCAGC 2 cut(s) 160, 719
BsgI GTGCAG 1 cut(s) 59
BshFI GGCC 3 cut(s) 345, 367, 712
BshNI GGYRCC 1 cut(s) 661
BsiSI CCGG 1 cut(s) 58
BsmI GAATGC 1 cut(s) 817
BsnI GGCC 3 cut(s) 345, 367, 712
Bsp1286I GDGCHC 2 cut(s) 498, 863
Bsp143I GATC 3 cut(s) 103, 201, 385
BspACI CCGC 4 cut(s) 155, 257, 324, 507
BspANI GGCC 3 cut(s) 345, 367, 712
BspCNI CTCAG 3 cut(s) 127, 427, 875
BspHI TCATGA 1 cut(s) 330
BspLI GGNNCC 2 cut(s) 663, 762
BspT107I GGYRCC 1 cut(s) 661
BsrDI GCAATG 2 cut(s) 557, 658
BsrI ACTGG 1 cut(s) 235
BssECI CCNNGG 2 cut(s) 531, 567
BssMI GATC 3 cut(s) 103, 201, 385
Bst2UI CCWGG 3 cut(s) 532, 569, 659
Bst4CI ACNGT 1 cut(s) 562
Bst6I CTCTTC 1 cut(s) 229
BstC8I GCNNGC 1 cut(s) 548
BstDEI CTNAG 6 cut(s) 19, 135, 414, 779, 801, 862
BstF5I GGATG 3 cut(s) 265, 409, 472
BstKTI GATC 3 cut(s) 106, 204, 388
BstMBI GATC 3 cut(s) 103, 201, 385
BstMWI GCNNNNNNNGC 2 cut(s) 552, 709
BstNI CCWGG 3 cut(s) 532, 569, 659
BstSCI CCNGG 3 cut(s) 530, 567, 657
BstSLI GKGCMC 1 cut(s) 498
BstV1I GCAGC 2 cut(s) 160, 719
BstX2I RGATCY 1 cut(s) 201
BstXI CCANNNNNNTGG 1 cut(s) 538
BstYI RGATCY 1 cut(s) 201
Bsu36I CCTNAGG 1 cut(s) 135
BsuI GTATCC 1 cut(s) 133
BsuRI GGCC 3 cut(s) 345, 367, 712
BtsCI GGATG 3 cut(s) 265, 409, 472
BtsI GCAGTG 2 cut(s) 240, 822
BtsIMutI CAGTG 4 cut(s) 86, 228, 240, 822
Cac8I GCNNGC 1 cut(s) 548
CciI TCATGA 1 cut(s) 330
Cfr13I GGNCC 1 cut(s) 710
CseI GACGC 1 cut(s) 325
CsiI ACCWGGT 1 cut(s) 657
CviAII CATG 5 cut(s) 169, 264, 288, 331, 556
DdeI CTNAG 6 cut(s) 19, 135, 414, 779, 801, 862
DpnI GATC 3 cut(s) 105, 203, 387
DpnII GATC 3 cut(s) 103, 201, 385
DraI TTTAAA 1 cut(s) 27
DrdI GACNNNNNNGTC 1 cut(s) 81
DseDI GACNNNNNNGTC 1 cut(s) 81
EaeI YGGCCR 1 cut(s) 365
Eam1104I CTCTTC 1 cut(s) 229
EarI CTCTTC 1 cut(s) 229
Eco24I GRGCYC 1 cut(s) 863
Eco81I CCTNAGG 1 cut(s) 135
EcoRII CCWGG 3 cut(s) 530, 567, 657
EcoT22I ATGCAT 2 cut(s) 265, 557
EcoT38I GRGCYC 1 cut(s) 863
FaeI CATG 5 cut(s) 172, 267, 291, 334, 559
FatI CATG 5 cut(s) 168, 263, 287, 330, 555
FauI CCCGC 1 cut(s) 500
FbaI TGATCA 1 cut(s) 385
Fnu4HI GCNGC 4 cut(s) 156, 174, 325, 733
FokI GGATG 3 cut(s) 272, 396, 479
FriOI GRGCYC 1 cut(s) 863
Fsp4HI GCNGC 4 cut(s) 156, 174, 325, 733
FspBI CTAG 2 cut(s) 377, 666
GluI GCNGC 4 cut(s) 156, 174, 325, 733
HaeIII GGCC 3 cut(s) 345, 367, 712
HapII CCGG 1 cut(s) 58
HgaI GACGC 1 cut(s) 325
Hin1II CATG 5 cut(s) 172, 267, 291, 334, 559
HinfI GANTC 2 cut(s) 61, 90
HpaII CCGG 1 cut(s) 58
HphI GGTGA 2 cut(s) 217, 395
Hpy166II GTNNAC 1 cut(s) 646
Hpy188I TCNGA 2 cut(s) 437, 865
Hpy188III TCNNGA 2 cut(s) 94, 331
Hpy8I GTNNAC 1 cut(s) 646
Hpy99I CGWCG 1 cut(s) 220
HpyAV CCTTC 3 cut(s) 595, 700, 748
HpyCH4III ACNGT 1 cut(s) 562
HpyCH4V TGCA 5 cut(s) 40, 263, 460, 555, 815
HpyF10VI GCNNNNNNNGC 2 cut(s) 552, 709
HpyF3I CTNAG 6 cut(s) 19, 135, 414, 779, 801, 862
Hsp92II CATG 5 cut(s) 172, 267, 291, 334, 559
Ksp22I TGATCA 1 cut(s) 385
Kzo9I GATC 3 cut(s) 103, 201, 385
LmnI GCTCC 3 cut(s) 250, 823, 834
Lsp1109I GCAGC 2 cut(s) 160, 719
LweI GCATC 3 cut(s) 135, 244, 250
MabI ACCWGGT 1 cut(s) 657
MaeI CTAG 2 cut(s) 377, 666
MaeIII GTNAC 2 cut(s) 401, 562
MalI GATC 3 cut(s) 105, 203, 387
MboI GATC 3 cut(s) 103, 201, 385
MboII GAAGA 1 cut(s) 246
MflI RGATCY 1 cut(s) 201
MhlI GDGCHC 2 cut(s) 498, 863
MlsI TGGCCA 1 cut(s) 367
MluCI AATT 2 cut(s) 108, 113
MluNI TGGCCA 1 cut(s) 367
MmeI TCCRAC 1 cut(s) 553
Mox20I TGGCCA 1 cut(s) 367
Mph1103I ATGCAT 2 cut(s) 265, 557
MscI TGGCCA 1 cut(s) 367
MseI TTAA 3 cut(s) 26, 221, 830
MslI CAYNNNNRTG 2 cut(s) 35, 560
Msp20I TGGCCA 1 cut(s) 367
MspA1I CMGCKG 2 cut(s) 324, 732
MspI CCGG 1 cut(s) 58
MspR9I CCNGG 3 cut(s) 532, 569, 659
Mva1269I GAATGC 1 cut(s) 817
MvaI CCWGG 3 cut(s) 532, 569, 659
MwoI GCNNNNNNNGC 2 cut(s) 552, 709
NdeII GATC 3 cut(s) 103, 201, 385
NlaIII CATG 5 cut(s) 172, 267, 291, 334, 559
NlaIV GGNNCC 2 cut(s) 663, 762
NmeAIII GCCGAG 1 cut(s) 321
NmuCI GTSAC 2 cut(s) 401, 562
NsiI ATGCAT 2 cut(s) 265, 557
PagI TCATGA 1 cut(s) 330
PcsI WCGNNNNNNNCGW 1 cut(s) 212
PctI GAATGC 1 cut(s) 817
PfeI GAWTC 2 cut(s) 61, 90
PkrI GCNGC 4 cut(s) 157, 175, 326, 734
Psp6I CCWGG 3 cut(s) 530, 567, 657
PspGI CCWGG 3 cut(s) 530, 567, 657
PspN4I GGNNCC 2 cut(s) 663, 762
PspPI GGNCC 1 cut(s) 710
PsuI RGATCY 1 cut(s) 201
PvuII CAGCTG 1 cut(s) 732
RseI CAYNNNNRTG 2 cut(s) 35, 560
SaqAI TTAA 3 cut(s) 26, 221, 830
SatI GCNGC 4 cut(s) 156, 174, 325, 733
Sau3AI GATC 3 cut(s) 103, 201, 385
Sau96I GGNCC 1 cut(s) 710
ScrFI CCNGG 3 cut(s) 532, 569, 659
SduI GDGCHC 2 cut(s) 498, 863
SexAI ACCWGGT 1 cut(s) 657
SfaNI GCATC 3 cut(s) 135, 244, 250
SmiMI CAYNNNNRTG 2 cut(s) 35, 560
Sse9I AATT 2 cut(s) 108, 113
SsiI CCGC 4 cut(s) 155, 257, 324, 507
SspMI CTAG 2 cut(s) 377, 666
StyD4I CCNGG 3 cut(s) 530, 567, 657
TaaI ACNGT 1 cut(s) 562
TaqI TCGA 4 cut(s) 53, 73, 106, 279
TasI AATT 2 cut(s) 108, 113
TauI GCSGC 2 cut(s) 158, 327
TfiI GAWTC 2 cut(s) 61, 90
Tru1I TTAA 3 cut(s) 26, 221, 830
Tru9I TTAA 3 cut(s) 26, 221, 830
TscAI CASTG 4 cut(s) 86, 235, 247, 822
TseFI GTSAC 2 cut(s) 401, 562
TseI GCWGC 2 cut(s) 173, 732
Tsp45I GTSAC 2 cut(s) 401, 562
TspDTI ATGAA 6 cut(s) 53, 319, 347, 614, 784, 829
TspGWI ACGGA 1 cut(s) 204
TspRI CASTG 4 cut(s) 86, 235, 247, 822
XcmI CCANNNNNNNNNTGG 2 cut(s) 360, 575
XspI CTAG 2 cut(s) 377, 666
Zsp2I ATGCAT 2 cut(s) 265, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.