RchiOBHm_Chr5g0011891

Plug domain of Sec61p

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
N/A
Physical Location & Seq
Reverse (-)
7917270 .. 7919014
1745 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 558 bp
ATGTCAGGAGAATTTAAAGTGCTGCATTTGGTCAGACCGTTTCTTCCGTTTCTACCGGAGGTTGAAAGTGCTAACAGAAAGGTCCACTTGTCTGAGAAGTTGATATATACCTTGTTTGCCCTAGGCATCTTTCTGGTGTGCAGTCAGCTTCCACTGTATGGAATACACTCTACAACTGGTGCTGATCCGTTTTACTGGATTCGTGTTATAATCGCATCTAGCCGCGGGACTGTAATGGAGCTTGGTATTAGCCCAATTGTGACATCTGGAATGGTGATGCAACTCTTGGCTGGTTCAAAGATGATTAAAGTGGACAACAATGTCCGTGAGGATCGTGCACTATTAAACGGGGCACAAAAGTTACTCGGAATCCTCATTGCTGTTGGTCAAGCTGTTGCCTATGTCCTATCAGGAATGTATGGTCCTGTTGGTCAGCTCGGCGTTGGGAATGCCATTCTTATCATAACTCAACTTTGCTTTGCTGCAATTATTGTGATGTGCCTAGATGAACTTCTTCAGAAGGGATATGGTCTTGGATCGGGATCTCCCTTCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 ko04141 ko04145 ko05110 map03060 map04141 map04145 map05110
Pfam Domains
Protein Families
Plug_translocon SecY

Protein Analysis

185

Amino Acids

19.88

Weight (kDa)

8.46

Isoelectric Point (pI)

26.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plug_translocon PF10559 43 - 76 4.5e-16 Plug domain of Sec61p
SecY PF00344 77 - 182 1.7e-24 SecY
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 209
AasI GACNNNNNNGTC 1 cut(s) 320
AccB7I CCANNNNNTGG 1 cut(s) 158
AccII CGCG 1 cut(s) 225
AciI CCGC 2 cut(s) 223, 225
AclWI GGATC 4 cut(s) 179, 339, 544, 550
AcsI RAATTY 1 cut(s) 11
AcuI CTGAAG 1 cut(s) 500
AfiI CCNNNNNNNGG 1 cut(s) 158
AgsI TTSAA 2 cut(s) 65, 297
AluBI AGCT 4 cut(s) 148, 241, 392, 436
AluI AGCT 4 cut(s) 148, 241, 392, 436
Alw21I GWGCWC 1 cut(s) 340
Alw44I GTGCAC 1 cut(s) 336
AlwI GGATC 4 cut(s) 179, 339, 544, 550
ApaLI GTGCAC 1 cut(s) 336
ApeKI GCWGC 2 cut(s) 22, 482
ApoI RAATTY 1 cut(s) 11
Asp700I GAANNNNTTC 1 cut(s) 513
AspA2I CCTAGG 1 cut(s) 121
AspS9I GGNCC 2 cut(s) 82, 422
AsuHPI GGTGA 1 cut(s) 286
AvaII GGWCC 2 cut(s) 82, 422
AvrII CCTAGG 1 cut(s) 121
BaeGI GKGCMC 2 cut(s) 340, 355
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 2 cut(s) 9, 469
BfaI CTAG 3 cut(s) 122, 219, 503
BisI GCNGC 3 cut(s) 23, 223, 483
BlnI CCTAGG 1 cut(s) 121
BlsI GCNGC 3 cut(s) 24, 224, 484
Bme18I GGWCC 2 cut(s) 82, 422
BmgT120I GGNCC 2 cut(s) 82, 422
BmsI GCATC 3 cut(s) 135, 224, 267
BsaBI GATNNNNATC 1 cut(s) 541
BsaJI CCNNGG 2 cut(s) 121, 223
BsaWI WCCGGW 1 cut(s) 55
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse1I ACTGG 2 cut(s) 181, 200
Bse3DI GCAATG 1 cut(s) 375
Bse8I GATNNNNATC 1 cut(s) 541
BseDI CCNNGG 2 cut(s) 121, 223
BseJI GATNNNNATC 1 cut(s) 541
BseLI CCNNNNNNNGG 1 cut(s) 158
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 1 cut(s) 84
BseNI ACTGG 2 cut(s) 181, 200
BseSI GKGCMC 2 cut(s) 340, 355
BseXI GCAGC 2 cut(s) 9, 469
BsgI GTGCAG 1 cut(s) 160
Bsh1236I CGCG 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 340
BsiSI CCGG 1 cut(s) 56
BslFI GGGAC 1 cut(s) 241
BslI CCNNNNNNNGG 1 cut(s) 158
BsmFI GGGAC 1 cut(s) 241
BsmI GAATGC 1 cut(s) 454
Bsp1286I GDGCHC 2 cut(s) 340, 355
Bsp143I GATC 4 cut(s) 184, 331, 536, 542
BspACI CCGC 2 cut(s) 223, 225
BspCNI CTCAG 1 cut(s) 85
BspFNI CGCG 1 cut(s) 225
BspPI GGATC 4 cut(s) 179, 339, 544, 550
BsrDI GCAATG 1 cut(s) 375
BsrI ACTGG 2 cut(s) 181, 200
BssECI CCNNGG 2 cut(s) 121, 223
BssMI GATC 4 cut(s) 184, 331, 536, 542
BssT1I CCWWGG 1 cut(s) 121
Bst4CI ACNGT 3 cut(s) 39, 156, 232
BstDEI CTNAG 1 cut(s) 93
BstDSI CCRYGG 1 cut(s) 223
BstFNI CGCG 1 cut(s) 225
BstKTI GATC 4 cut(s) 187, 334, 539, 545
BstMBI GATC 4 cut(s) 184, 331, 536, 542
BstSLI GKGCMC 2 cut(s) 340, 355
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 2 cut(s) 9, 469
BstX2I RGATCY 1 cut(s) 542
BstYI RGATCY 1 cut(s) 542
BtgI CCRYGG 1 cut(s) 223
BtsIMutI CAGTG 1 cut(s) 152
Cfr13I GGNCC 2 cut(s) 82, 422
Cfr42I CCGCGG 1 cut(s) 226
CviJI RGCY 7 cut(s) 148, 222, 241, 252, 290, 392, 436
CviKI_1 RGCY 7 cut(s) 148, 222, 241, 252, 290, 392, 436
DdeI CTNAG 1 cut(s) 93
DpnI GATC 4 cut(s) 186, 333, 538, 544
DpnII GATC 4 cut(s) 184, 331, 536, 542
DraI TTTAAA 1 cut(s) 16
DrdI GACNNNNNNGTC 1 cut(s) 320
DseDI GACNNNNNNGTC 1 cut(s) 320
Eco130I CCWWGG 1 cut(s) 121
Eco47I GGWCC 2 cut(s) 82, 422
Eco57I CTGAAG 1 cut(s) 500
EcoT14I CCWWGG 1 cut(s) 121
ErhI CCWWGG 1 cut(s) 121
FaiI YATR 9 cut(s) 106, 108, 159, 209, 402, 420, 464, 528, 556
FalI AAGNNNNNCTT 2 cut(s) 71, 103
FaqI GGGAC 1 cut(s) 241
FauI CCCGC 1 cut(s) 218
Fnu4HI GCNGC 3 cut(s) 23, 223, 483
Fsp4HI GCNGC 3 cut(s) 23, 223, 483
FspBI CTAG 3 cut(s) 122, 219, 503
GluI GCNGC 3 cut(s) 23, 223, 483
HapII CCGG 1 cut(s) 56
HinfI GANTC 2 cut(s) 199, 369
HpaII CCGG 1 cut(s) 56
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 3 cut(s) 85, 313, 338
Hpy188I TCNGA 4 cut(s) 35, 94, 368, 519
Hpy188III TCNNGA 4 cut(s) 6, 267, 411, 540
Hpy8I GTNNAC 3 cut(s) 85, 313, 338
HpyAV CCTTC 1 cut(s) 514
HpyCH4III ACNGT 3 cut(s) 39, 156, 232
HpyCH4V TGCA 5 cut(s) 25, 141, 280, 338, 485
HpyF3I CTNAG 1 cut(s) 93
KspI CCGCGG 1 cut(s) 226
Kzo9I GATC 4 cut(s) 184, 331, 536, 542
LmnI GCTCC 1 cut(s) 238
LpnPI CCDG 8 cut(s) 69, 119, 162, 181, 252, 276, 396, 438
Lsp1109I GCAGC 2 cut(s) 9, 469
LweI GCATC 3 cut(s) 135, 224, 267
MaeI CTAG 3 cut(s) 122, 219, 503
MaeIII GTNAC 2 cut(s) 259, 360
MalI GATC 4 cut(s) 186, 333, 538, 544
MboI GATC 4 cut(s) 184, 331, 536, 542
MboII GAAGA 2 cut(s) 35, 506
MfeI CAATTG 1 cut(s) 255
MflI RGATCY 1 cut(s) 542
MhlI GDGCHC 2 cut(s) 340, 355
MluCI AATT 3 cut(s) 11, 255, 486
MnlI CCTC 3 cut(s) 52, 322, 383
MroXI GAANNNNTTC 1 cut(s) 513
MseI TTAA 3 cut(s) 15, 306, 344
MspA1I CMGCKG 1 cut(s) 225
MspI CCGG 1 cut(s) 56
MunI CAATTG 1 cut(s) 255
Mva1269I GAATGC 1 cut(s) 454
MvnI CGCG 1 cut(s) 225
NdeII GATC 4 cut(s) 184, 331, 536, 542
NmeAIII GCCGAG 1 cut(s) 417
NmuCI GTSAC 1 cut(s) 259
PctI GAATGC 1 cut(s) 454
PdmI GAANNNNTTC 1 cut(s) 513
PfeI GAWTC 2 cut(s) 199, 369
PflMI CCANNNNNTGG 1 cut(s) 158
PkrI GCNGC 3 cut(s) 24, 224, 484
PsiI TTATAA 1 cut(s) 209
PspPI GGNCC 2 cut(s) 82, 422
PsuI RGATCY 1 cut(s) 542
SacII CCGCGG 1 cut(s) 226
SaqAI TTAA 3 cut(s) 15, 306, 344
SatI GCNGC 3 cut(s) 23, 223, 483
Sau3AI GATC 4 cut(s) 184, 331, 536, 542
Sau96I GGNCC 2 cut(s) 82, 422
SduI GDGCHC 2 cut(s) 340, 355
SetI ASST 7 cut(s) 63, 84, 113, 150, 243, 394, 438
SfaNI GCATC 3 cut(s) 135, 224, 267
Sfr303I CCGCGG 1 cut(s) 226
SgrBI CCGCGG 1 cut(s) 226
SinI GGWCC 2 cut(s) 82, 422
Sse9I AATT 3 cut(s) 11, 255, 486
SsiI CCGC 2 cut(s) 223, 225
SspMI CTAG 3 cut(s) 122, 219, 503
StyI CCWWGG 1 cut(s) 121
TaaI ACNGT 3 cut(s) 39, 156, 232
TasI AATT 3 cut(s) 11, 255, 486
TauI GCSGC 1 cut(s) 225
TfiI GAWTC 2 cut(s) 199, 369
Tru1I TTAA 3 cut(s) 15, 306, 344
Tru9I TTAA 3 cut(s) 15, 306, 344
TscAI CASTG 1 cut(s) 159
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 2 cut(s) 22, 482
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 1 cut(s) 522
TspGWI ACGGA 3 cut(s) 36, 177, 314
TspRI CASTG 1 cut(s) 159
Van91I CCANNNNNTGG 1 cut(s) 158
VneI GTGCAC 1 cut(s) 336
VpaK11BI GGWCC 2 cut(s) 82, 422
XapI RAATTY 1 cut(s) 11
XmaJI CCTAGG 1 cut(s) 121
XmnI GAANNNNTTC 1 cut(s) 513
XspI CTAG 3 cut(s) 122, 219, 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.