RchiOBHm_Chr5g0013191

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
N/A
Physical Location & Seq
Forward (+)
8938171 .. 8939233
1063 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 915 bp
ATGTCGATTTATACAGTCTTTGTCCTCGCTGGCCAGGCAATTGCAACACTATTGGGAAGACAGTACTATGACAAAGGTGGTAAAAGCAACTGGCTTGCTACACTAGTACAACTTTGTGGCTTTCCTATCTTCCTTCCGTATTATTGCATTCCAGCATTGAACAAAAACTGTCCAACCACAAACAATCGAGTCAAAGCCACTAGCAATCGAGTCAAAGCCACTGTCTATATTAGTCCTTGCATCAATTTATGCCTCTCTTGGCTCCCTTATAGCAGCAGACTACTATTTGTATTCAGTAGGACTATCTTACCTTCCTGTATCTACTTTCTCCCTCATTTCTGCATCCCAACTCACCTTCAATGCACTTTTCTCCTTCTTCCTCAACACGCAGAAGTTAACTTTTTTAATTGTCAACTCTCTTTTATACTGACCATCTCATCCAGCCTCCTAGTGCTTCAAGCAGACCACTCTGCAGACTCGAGTAAAGTTTCCAGGTCAAAGTACATAATAGGCTTTGTCTGCACTGTTGGGGCATCGGCTGGATATGCATTGACACTTTCTCTAACGCAGCTAGTCTTCATAAAGGTTATAAGAAAAGAAACATTTAGAGTGATCATGGATATGAGTCATATGACAGTGTATACATCAGTCATTGCAACCGCTATTATCTTGGCGTCATCCCTCTTCTCGAATTCTATAAGTGCTGTGGGTTTGCCTATTGTTCCAGTTCTAGCTGTGATCTTCTTCCATGAAAAAATGGATGGGCTAAAGGCAATGTCCCTGGTTTTGGCTATGTGGGGTTTTATTTCATATGTTTATCAGGACTACCTCGATGATCGTAAGTCCAAGAGTGAGAAGAAAGATGCCAATGATGAAGTTTCTAAAGCTGTCCCTGTGGAAAACATTAATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

304

Amino Acids

33.83

Weight (kDa)

9.02

Isoelectric Point (pI)

40.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 2 - 223 6.3e-35 Purine nucleobase transmembrane transport
PUNUT PF16913 220 - 281 2.1e-20 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 590
AccI GTMKAC 1 cut(s) 641
AciI CCGC 1 cut(s) 660
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 1 cut(s) 691
AcyI GRCGYC 1 cut(s) 674
AfaI GTAC 3 cut(s) 65, 108, 503
AfiI CCNNNNNNNGG 1 cut(s) 787
AgsI TTSAA 3 cut(s) 160, 359, 458
AhlI ACTAGT 1 cut(s) 103
AjnI CCWGG 3 cut(s) 33, 491, 780
AluBI AGCT 3 cut(s) 571, 734, 887
AluI AGCT 3 cut(s) 571, 734, 887
Ama87I CYCGRG 1 cut(s) 478
AoxI GGCC 1 cut(s) 31
ApeKI GCWGC 2 cut(s) 273, 568
ApoI RAATTY 1 cut(s) 691
ArsI GACNNNNNNTTYG 2 cut(s) 207, 239
AseI ATTAAT 1 cut(s) 906
AsuHPI GGTGA 1 cut(s) 344
AvaI CYCGRG 1 cut(s) 478
BalI TGGCCA 1 cut(s) 33
BbsI GAAGAC 2 cut(s) 64, 568
BbvI GCAGC 2 cut(s) 285, 580
BccI CCATC 2 cut(s) 440, 755
BciT130I CCWGG 3 cut(s) 35, 493, 782
BclI TGATCA 1 cut(s) 612
BcuI ACTAGT 1 cut(s) 103
BfaI CTAG 5 cut(s) 104, 201, 449, 572, 731
BfmI CTRYAG 1 cut(s) 471
BisI GCNGC 2 cut(s) 274, 569
BlsI GCNGC 2 cut(s) 275, 570
BmcAI AGTACT 1 cut(s) 65
Bme1390I CCNGG 3 cut(s) 35, 493, 782
BmeT110I CYCGRG 1 cut(s) 478
BmiI GGNNCC 1 cut(s) 263
BmrFI CCNGG 3 cut(s) 35, 493, 782
BmsI GCATC 4 cut(s) 249, 351, 542, 853
BpiI GAAGAC 2 cut(s) 64, 568
BsaHI GRCGYC 1 cut(s) 674
BsaJI CCNNGG 1 cut(s) 780
Bsc4I CCNNNNNNNGG 1 cut(s) 787
Bse1I ACTGG 2 cut(s) 95, 725
Bse3DI GCAATG 2 cut(s) 651, 780
BseBI CCWGG 3 cut(s) 35, 493, 782
BseDI CCNNGG 1 cut(s) 780
BseGI GGATG 4 cut(s) 342, 437, 677, 766
BseLI CCNNNNNNNGG 1 cut(s) 787
BseMI GCAATG 2 cut(s) 651, 780
BseNI ACTGG 2 cut(s) 95, 725
BseXI GCAGC 2 cut(s) 285, 580
BsgI GTGCAG 1 cut(s) 505
BshFI GGCC 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 478
BslFI GGGAC 2 cut(s) 763, 875
BslI CCNNNNNNNGG 1 cut(s) 787
BsmFI GGGAC 2 cut(s) 763, 875
BsmI GAATGC 1 cut(s) 147
BsnI GGCC 1 cut(s) 33
BsoBI CYCGRG 1 cut(s) 478
Bsp143I GATC 3 cut(s) 612, 738, 835
BspACI CCGC 1 cut(s) 660
BspANI GGCC 1 cut(s) 33
BspLI GGNNCC 1 cut(s) 263
BspMAI CTGCAG 1 cut(s) 475
BsrDI GCAATG 2 cut(s) 651, 780
BsrI ACTGG 2 cut(s) 95, 725
BssECI CCNNGG 1 cut(s) 780
BssMI GATC 3 cut(s) 612, 738, 835
BssNAI GTATAC 1 cut(s) 642
BssNI GRCGYC 1 cut(s) 674
Bst1107I GTATAC 1 cut(s) 642
Bst2UI CCWGG 3 cut(s) 35, 493, 782
Bst4CI ACNGT 6 cut(s) 16, 63, 170, 223, 526, 637
Bst6I CTCTTC 1 cut(s) 689
BstACI GRCGYC 1 cut(s) 674
BstC8I GCNNGC 2 cut(s) 31, 96
BstF5I GGATG 4 cut(s) 342, 437, 677, 766
BstKTI GATC 3 cut(s) 615, 741, 838
BstMBI GATC 3 cut(s) 612, 738, 835
BstMWI GCNNNNNNNGC 3 cut(s) 35, 519, 545
BstNI CCWGG 3 cut(s) 35, 493, 782
BstSCI CCNGG 3 cut(s) 33, 491, 780
BstSFI CTRYAG 1 cut(s) 471
BstV1I GCAGC 2 cut(s) 285, 580
BstV2I GAAGAC 2 cut(s) 64, 568
BstZ17I GTATAC 1 cut(s) 642
BsuRI GGCC 1 cut(s) 33
BtsCI GGATG 4 cut(s) 342, 437, 677, 766
BtsIMutI CAGTG 3 cut(s) 219, 522, 642
Cac8I GCNNGC 2 cut(s) 31, 96
CseI GACGC 1 cut(s) 663
Csp6I GTAC 3 cut(s) 64, 107, 502
CviAII CATG 2 cut(s) 616, 749
CviQI GTAC 3 cut(s) 64, 107, 502
DpnI GATC 3 cut(s) 614, 740, 837
DpnII GATC 3 cut(s) 612, 738, 835
EaeI YGGCCR 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 689
EarI CTCTTC 1 cut(s) 689
Eco88I CYCGRG 1 cut(s) 478
EcoRI GAATTC 1 cut(s) 691
EcoRII CCWGG 3 cut(s) 33, 491, 780
EcoT22I ATGCAT 1 cut(s) 550
FaeI CATG 2 cut(s) 619, 752
FaqI GGGAC 2 cut(s) 763, 875
FatI CATG 2 cut(s) 615, 748
FauNDI CATATG 2 cut(s) 630, 811
FbaI TGATCA 1 cut(s) 612
FblI GTMKAC 1 cut(s) 641
Fnu4HI GCNGC 2 cut(s) 274, 569
FokI GGATG 4 cut(s) 329, 424, 664, 773
Fsp4HI GCNGC 2 cut(s) 274, 569
FspBI CTAG 5 cut(s) 104, 201, 449, 572, 731
GluI GCNGC 2 cut(s) 274, 569
HaeIII GGCC 1 cut(s) 33
HgaI GACGC 1 cut(s) 663
Hin1I GRCGYC 1 cut(s) 674
Hin1II CATG 2 cut(s) 619, 752
HincII GTYRAC 2 cut(s) 397, 413
HindII GTYRAC 2 cut(s) 397, 413
HinfI GANTC 4 cut(s) 189, 210, 476, 625
HpaI GTTAAC 1 cut(s) 397
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 3 cut(s) 397, 413, 642
Hpy188III TCNNGA 2 cut(s) 688, 821
Hpy8I GTNNAC 3 cut(s) 397, 413, 642
HpyAV CCTTC 4 cut(s) 143, 321, 365, 383
HpyCH4III ACNGT 6 cut(s) 16, 63, 170, 223, 526, 637
HpyCH4V TGCA 9 cut(s) 44, 147, 240, 342, 363, 473, 522, 548, 656
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 519, 545
Hsp92I GRCGYC 1 cut(s) 674
Hsp92II CATG 2 cut(s) 619, 752
Ksp22I TGATCA 1 cut(s) 612
KspAI GTTAAC 1 cut(s) 397
Kzo9I GATC 3 cut(s) 612, 738, 835
LmnI GCTCC 1 cut(s) 267
Lsp1109I GCAGC 2 cut(s) 285, 580
LweI GCATC 4 cut(s) 249, 351, 542, 853
MaeI CTAG 5 cut(s) 104, 201, 449, 572, 731
MalI GATC 3 cut(s) 614, 740, 837
MboI GATC 3 cut(s) 612, 738, 835
MboII GAAGA 8 cut(s) 69, 121, 368, 568, 676, 733, 736, 868
MfeI CAATTG 1 cut(s) 39
MlsI TGGCCA 1 cut(s) 33
MluCI AATT 4 cut(s) 39, 244, 406, 691
MluNI TGGCCA 1 cut(s) 33
MlyI GAGTC 4 cut(s) 198, 219, 470, 634
MmeI TCCRAC 1 cut(s) 197
MnlI CCTC 7 cut(s) 35, 263, 342, 390, 455, 692, 839
Mox20I TGGCCA 1 cut(s) 33
Mph1103I ATGCAT 1 cut(s) 550
MscI TGGCCA 1 cut(s) 33
MseI TTAA 3 cut(s) 396, 405, 906
MslI CAYNNNNRTG 1 cut(s) 620
Msp20I TGGCCA 1 cut(s) 33
MspR9I CCNGG 3 cut(s) 35, 493, 782
MunI CAATTG 1 cut(s) 39
Mva1269I GAATGC 1 cut(s) 147
MvaI CCWGG 3 cut(s) 35, 493, 782
MwoI GCNNNNNNNGC 3 cut(s) 35, 519, 545
NdeI CATATG 2 cut(s) 630, 811
NdeII GATC 3 cut(s) 612, 738, 835
NlaIII CATG 2 cut(s) 619, 752
NlaIV GGNNCC 1 cut(s) 263
NsiI ATGCAT 1 cut(s) 550
PaeR7I CTCGAG 1 cut(s) 478
PctI GAATGC 1 cut(s) 147
PkrI GCNGC 2 cut(s) 275, 570
PleI GAGTC 4 cut(s) 197, 218, 470, 633
PpsI GAGTC 4 cut(s) 197, 218, 470, 633
PshBI ATTAAT 1 cut(s) 906
PsiI TTATAA 1 cut(s) 590
Psp6I CCWGG 3 cut(s) 33, 491, 780
PspGI CCWGG 3 cut(s) 33, 491, 780
PspN4I GGNNCC 1 cut(s) 263
PspXI VCTCGAGB 1 cut(s) 478
PstI CTGCAG 1 cut(s) 475
RsaI GTAC 3 cut(s) 65, 108, 503
RsaNI GTAC 3 cut(s) 64, 107, 502
RseI CAYNNNNRTG 1 cut(s) 620
SaqAI TTAA 3 cut(s) 396, 405, 906
SatI GCNGC 2 cut(s) 274, 569
Sau3AI GATC 3 cut(s) 612, 738, 835
ScaI AGTACT 1 cut(s) 65
SchI GAGTC 4 cut(s) 198, 219, 470, 634
ScrFI CCNGG 3 cut(s) 35, 493, 782
SetI ASST 9 cut(s) 79, 313, 357, 497, 573, 588, 736, 831, 889
SfaNI GCATC 4 cut(s) 249, 351, 542, 853
SfcI CTRYAG 1 cut(s) 471
Sfr274I CTCGAG 1 cut(s) 478
SlaI CTCGAG 1 cut(s) 478
SmiMI CAYNNNNRTG 1 cut(s) 620
SmlI CTYRAG 1 cut(s) 478
SmoI CTYRAG 1 cut(s) 478
SpeI ACTAGT 1 cut(s) 103
Sse9I AATT 4 cut(s) 39, 244, 406, 691
SsiI CCGC 1 cut(s) 660
SspMI CTAG 5 cut(s) 104, 201, 449, 572, 731
StyD4I CCNGG 3 cut(s) 33, 491, 780
TaaI ACNGT 6 cut(s) 16, 63, 170, 223, 526, 637
TaqI TCGA 6 cut(s) 5, 187, 208, 479, 689, 831
TasI AATT 4 cut(s) 39, 244, 406, 691
TatI WGTACW 3 cut(s) 63, 106, 501
Tru1I TTAA 3 cut(s) 396, 405, 906
Tru9I TTAA 3 cut(s) 396, 405, 906
TscAI CASTG 3 cut(s) 226, 529, 642
TseI GCWGC 2 cut(s) 273, 568
TspDTI ATGAA 4 cut(s) 568, 765, 798, 888
TspGWI ACGGA 1 cut(s) 126
TspRI CASTG 3 cut(s) 226, 529, 642
VspI ATTAAT 1 cut(s) 906
XapI RAATTY 1 cut(s) 691
XhoI CTCGAG 1 cut(s) 478
XmiI GTMKAC 1 cut(s) 641
XspI CTAG 5 cut(s) 104, 201, 449, 572, 731
ZrmI AGTACT 1 cut(s) 65
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.