RchiOBHm_Chr5g0035841

Phosphoenolpyruvate carboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
N/A
Physical Location & Seq
Forward (+)
29865777 .. 29866795
1019 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 402 bp
ATGGTGATTGAAGATCTGGTGCGAGAGATACTTCTGTATGGCAAACAGATGAGCTTAGGCACCATAAACCCACACCAGGTGATGAAGCTAGGGCAGGTAAACCACTTCAACATTAGATTCATTGATATTCATATGCTTTGGCTCGTTGAAATCGTGTGTATCTCTTACAGACAAAATAAAATTGTATATATTGTTTACCACAGGTTTGAACATTGTGGAGCGCAGTCCCTTTGGAAAGTTGTACCTCATTATTTACGTCGTGTCAACGATGCTTTAAAGAAGGTATGGGTCTTCAGAGGAGGGGAGGGTTGGTTTGATCGAGATGGAAATTCCAATGTGACAGCAATTTTTACTGTATTGATAATGGTGTTGGTAATTCATGGGGCAGTAAATCATGTTTGA

Protein Analysis

133

Amino Acids

15.57

Weight (kDa)

9.26

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 85
AccB1I GGYRCC 1 cut(s) 59
AcsI RAATTY 1 cut(s) 328
AcuI CTGAAG 1 cut(s) 277
AdeI CACNNNGTG 1 cut(s) 79
AfaI GTAC 1 cut(s) 243
AfiI CCNNNNNNNGG 1 cut(s) 76
AgsI TTSAA 4 cut(s) 11, 109, 149, 209
AjnI CCWGG 1 cut(s) 75
AluBI AGCT 2 cut(s) 54, 88
AluI AGCT 2 cut(s) 54, 88
ApoI RAATTY 1 cut(s) 328
AspLEI GCGC 1 cut(s) 223
AsuHPI GGTGA 2 cut(s) 16, 91
BaeI ACNNNNGTAYC 2 cut(s) 20, 53
BanI GGYRCC 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 283
BccI CCATC 1 cut(s) 317
BciT130I CCWGG 1 cut(s) 77
BfaI CTAG 1 cut(s) 89
BfuAI ACCTGC 1 cut(s) 85
BglII AGATCT 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 77
BmiI GGNNCC 1 cut(s) 61
BmrFI CCNGG 1 cut(s) 77
BmsI GCATC 1 cut(s) 259
BpiI GAAGAC 1 cut(s) 283
Bpu10I CCTNAGC 1 cut(s) 55
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 1 cut(s) 76
BseBI CCWGG 1 cut(s) 77
BseLI CCNNNNNNNGG 1 cut(s) 76
BseRI GAGGAG 1 cut(s) 312
BshNI GGYRCC 1 cut(s) 59
BslFI GGGAC 1 cut(s) 211
BslI CCNNNNNNNGG 1 cut(s) 76
BsmFI GGGAC 1 cut(s) 211
Bsp143I GATC 2 cut(s) 13, 316
BspLI GGNNCC 1 cut(s) 61
BspMI ACCTGC 1 cut(s) 85
BspT107I GGYRCC 1 cut(s) 59
BssMI GATC 2 cut(s) 13, 316
Bst2UI CCWGG 1 cut(s) 77
Bst4CI ACNGT 1 cut(s) 355
BstDEI CTNAG 1 cut(s) 55
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 2 cut(s) 16, 319
BstMBI GATC 2 cut(s) 13, 316
BstNI CCWGG 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 75
BstV2I GAAGAC 1 cut(s) 283
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BveI ACCTGC 1 cut(s) 85
CfoI GCGC 1 cut(s) 223
CsiI ACCWGGT 1 cut(s) 75
Csp6I GTAC 1 cut(s) 242
CviAII CATG 2 cut(s) 380, 395
CviJI RGCY 3 cut(s) 54, 88, 142
CviKI_1 RGCY 3 cut(s) 54, 88, 142
CviQI GTAC 1 cut(s) 242
DdeI CTNAG 1 cut(s) 55
DpnI GATC 2 cut(s) 15, 318
DpnII GATC 2 cut(s) 13, 316
DraI TTTAAA 1 cut(s) 276
DraIII CACNNNGTG 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 277
EcoRII CCWGG 1 cut(s) 75
FaeI CATG 2 cut(s) 383, 398
FaiI YATR 9 cut(s) 39, 65, 132, 134, 187, 189, 286, 381, 396
FaqI GGGAC 1 cut(s) 211
FatI CATG 2 cut(s) 379, 394
FauNDI CATATG 1 cut(s) 132
FspBI CTAG 1 cut(s) 89
GlaI GCGC 1 cut(s) 222
HhaI GCGC 1 cut(s) 223
Hin1II CATG 2 cut(s) 383, 398
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 1 cut(s) 117
HphI GGTGA 2 cut(s) 16, 91
Hpy166II GTNNAC 3 cut(s) 100, 196, 265
Hpy188I TCNGA 1 cut(s) 296
Hpy188III TCNNGA 1 cut(s) 320
Hpy8I GTNNAC 3 cut(s) 100, 196, 265
Hpy99I CGWCG 1 cut(s) 261
HpyAV CCTTC 1 cut(s) 274
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 1 cut(s) 256
HpyF3I CTNAG 1 cut(s) 55
HpySE526I ACGT 1 cut(s) 256
Hsp92II CATG 2 cut(s) 383, 398
HspAI GCGC 1 cut(s) 221
Kzo9I GATC 2 cut(s) 13, 316
LmnI GCTCC 1 cut(s) 218
LpnPI CCDG 5 cut(s) 2, 62, 80, 89, 187
LweI GCATC 1 cut(s) 259
MabI ACCWGGT 1 cut(s) 75
MaeI CTAG 1 cut(s) 89
MaeII ACGT 1 cut(s) 256
MaeIII GTNAC 1 cut(s) 337
MalI GATC 2 cut(s) 15, 318
MboI GATC 2 cut(s) 13, 316
MboII GAAGA 2 cut(s) 23, 283
MflI RGATCY 1 cut(s) 13
MluCI AATT 4 cut(s) 180, 328, 345, 375
MnlI CCTC 4 cut(s) 255, 290, 293, 298
MseI TTAA 1 cut(s) 275
MspR9I CCNGG 1 cut(s) 77
MvaI CCWGG 1 cut(s) 77
NdeI CATATG 1 cut(s) 132
NdeII GATC 2 cut(s) 13, 316
NlaIII CATG 2 cut(s) 383, 398
NlaIV GGNNCC 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 337
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PfeI GAWTC 1 cut(s) 117
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PspN4I GGNNCC 1 cut(s) 61
PsuI RGATCY 1 cut(s) 13
RsaI GTAC 1 cut(s) 243
RsaNI GTAC 1 cut(s) 242
SaqAI TTAA 1 cut(s) 275
Sau3AI GATC 2 cut(s) 13, 316
ScrFI CCNGG 1 cut(s) 77
SetI ASST 8 cut(s) 56, 81, 90, 99, 206, 247, 259, 285
SexAI ACCWGGT 1 cut(s) 75
SfaNI GCATC 1 cut(s) 259
Sse9I AATT 4 cut(s) 180, 328, 345, 375
SspMI CTAG 1 cut(s) 89
StyD4I CCNGG 1 cut(s) 75
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 1 cut(s) 259
TaqI TCGA 1 cut(s) 319
TasI AATT 4 cut(s) 180, 328, 345, 375
TfiI GAWTC 1 cut(s) 117
Tru1I TTAA 1 cut(s) 275
Tru9I TTAA 1 cut(s) 275
TseFI GTSAC 1 cut(s) 337
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 4 cut(s) 98, 109, 119, 368
XapI RAATTY 1 cut(s) 328
XspI CTAG 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.