RchiOBHm_Chr5g0039571

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0030192
Physical Location & Seq
Forward (+)
34317737 .. 34318487
751 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 573 bp
ATGGGTCCAGGCTCCCCTATCGACTTCGACCAAGGATGGGACGACATAGAGTGGGCTATCGTTAAGCTCACCCGAATCCTCGAGGGGTTGCCGGAGACGCCGTTCGACGCCGAATACCACATCTATGTCTACTCCACCGTCTGCAACATGTGCGACGATCACTCCCACCAGGTCTACGAGGCTTGTCGCGAGACCATCGAGGCCTACAACACTGAGATTGTGTTGCCTAGTTTGGCAGACAAGCACGGCGCGCTTTTGCTCCGGGAGTTGGTCAGAAGGTGGAGGAATAACAAGGCTCTGATGCGGTGGCTATGGCGTTTCTTTATTGTTCTTGATCAGTATTATGTTGAGAAAGCAAAGGTTCCTGGGATTAAGCAGGCTGGGATTATCGGGTTTCGCGACGAGGTTTATGAGAAGGTGAAGGAGAATGTTGGGGGTGCTGTGATGGGGATGATTAATGAGGAGAGGGAAGGGGGGCTGATTGACAGGGGACTGTTGAAAGATGTGGTGGAGATTTTTGTTAAAATGGGGATTTACGAAGCGGATTGTGAAGAGGAGATGATGAGGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000228 GO:0000793 GO:0000794 GO:0001101 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005819 GO:0005829 GO:0005856 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009507 GO:0009524 GO:0009526 GO:0009536 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009941 GO:0009987 GO:0010033 GO:0010087 GO:0010154 GO:0015630 GO:0019005 GO:0019899 GO:0022414 GO:0023052 GO:0031461 GO:0031625 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0042221 GO:0042752 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044389 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0099402 GO:1901700 GO:1901701 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04110 ko04111 ko04114 ko04120 ko04141 ko04310 ko04340 ko04341 ko04350 ko04710 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 ko05168 ko05200 map00190 map01100 map04110 map04111 map04114 map04120 map04141 map04310 map04340 map04341 map04350 map04710 map04714 map04723 map04932 map05010 map05012 map05016 map05168 map05200
Pfam Domains
Protein Families
Cullin Cullin_Nedd8

Protein Analysis

190

Amino Acids

22.06

Weight (kDa)

4.8

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 42 - 189 3.7e-25 Cullin alpha solenoid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030192)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0039571
rosa_multiflora Rmu_sc0001507.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 80
AccI GTMKAC 2 cut(s) 129, 174
AccII CGCG 3 cut(s) 189, 251, 399
AciI CCGC 2 cut(s) 304, 542
AcyI GRCGYC 2 cut(s) 98, 108
AfiI CCNNNNNNNGG 2 cut(s) 37, 268
AflIII ACRYGT 1 cut(s) 147
AgsI TTSAA 1 cut(s) 499
AjnI CCWGG 3 cut(s) 7, 168, 364
AloI GAACNNNNNNTCC 2 cut(s) 86, 118
AluBI AGCT 1 cut(s) 67
AluI AGCT 1 cut(s) 67
Alw26I GTCTC 2 cut(s) 89, 185
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 1 cut(s) 201
AseI ATTAAT 1 cut(s) 456
AspLEI GCGC 2 cut(s) 251, 253
AspS9I GGNCC 1 cut(s) 5
AsuC2I CCSGG 1 cut(s) 263
AsuHPI GGTGA 2 cut(s) 61, 430
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 1 cut(s) 5
BccI CCATC 3 cut(s) 30, 203, 439
BceAI ACGGC 2 cut(s) 85, 262
BciT130I CCWGG 3 cut(s) 9, 170, 366
BclI TGATCA 1 cut(s) 334
BcnI CCSGG 1 cut(s) 263
BcoDI GTCTC 2 cut(s) 89, 185
BfaI CTAG 1 cut(s) 228
Bme1390I CCNGG 4 cut(s) 9, 170, 263, 366
Bme18I GGWCC 1 cut(s) 5
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 5
BmiI GGNNCC 3 cut(s) 6, 13, 363
BmrFI CCNGG 4 cut(s) 9, 170, 263, 366
BmsI GCATC 1 cut(s) 291
BpuMI CCSGG 1 cut(s) 263
BsaHI GRCGYC 2 cut(s) 98, 108
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 2 cut(s) 31, 365
BsaXI ACNNNNNCTCC 4 cut(s) 86, 116, 146, 176
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 268
BseBI CCWGG 3 cut(s) 9, 170, 366
BseDI CCNNGG 2 cut(s) 31, 365
BseGI GGATG 2 cut(s) 41, 456
BseLI CCNNNNNNNGG 2 cut(s) 37, 268
BseMII CTCAG 1 cut(s) 204
BsePI GCGCGC 1 cut(s) 249
BseRI GAGGAG 2 cut(s) 476, 569
BseYI CCCAGC 1 cut(s) 380
Bsh1236I CGCG 3 cut(s) 189, 251, 399
BshFI GGCC 1 cut(s) 203
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 2 cut(s) 92, 262
BslFI GGGAC 2 cut(s) 53, 504
BslI CCNNNNNNNGG 2 cut(s) 37, 268
BsmAI GTCTC 2 cut(s) 89, 185
BsmBI CGTCTC 1 cut(s) 89
BsmFI GGGAC 2 cut(s) 53, 504
BsnI GGCC 1 cut(s) 203
Bso31I GGTCTC 1 cut(s) 185
BsoBI CYCGRG 1 cut(s) 80
Bsp143I GATC 2 cut(s) 157, 334
Bsp68I TCGCGA 2 cut(s) 189, 399
BspACI CCGC 2 cut(s) 304, 542
BspANI GGCC 1 cut(s) 203
BspCNI CTCAG 1 cut(s) 205
BspFNI CGCG 3 cut(s) 189, 251, 399
BspLI GGNNCC 3 cut(s) 6, 13, 363
BspTNI GGTCTC 1 cut(s) 185
BssECI CCNNGG 2 cut(s) 31, 365
BssHII GCGCGC 1 cut(s) 249
BssMI GATC 2 cut(s) 157, 334
BssNI GRCGYC 2 cut(s) 98, 108
BssT1I CCWWGG 1 cut(s) 31
Bst2UI CCWGG 3 cut(s) 9, 170, 366
Bst4CI ACNGT 2 cut(s) 139, 495
Bst6I CTCTTC 1 cut(s) 546
BstACI GRCGYC 2 cut(s) 98, 108
BstAPI GCANNNNNTGC 1 cut(s) 150
BstC8I GCNNGC 2 cut(s) 251, 378
BstDEI CTNAG 1 cut(s) 213
BstF5I GGATG 2 cut(s) 41, 456
BstFNI CGCG 3 cut(s) 189, 251, 399
BstHHI GCGC 2 cut(s) 251, 253
BstKTI GATC 2 cut(s) 160, 337
BstMAI GTCTC 2 cut(s) 89, 185
BstMBI GATC 2 cut(s) 157, 334
BstMWI GCNNNNNNNGC 3 cut(s) 97, 150, 250
BstNI CCWGG 3 cut(s) 9, 170, 366
BstNSI RCATGY 1 cut(s) 151
BstSCI CCNGG 4 cut(s) 7, 168, 261, 364
BstUI CGCG 3 cut(s) 189, 251, 399
BsuRI GGCC 1 cut(s) 203
BtsCI GGATG 2 cut(s) 41, 456
BtsIMutI CAGTG 1 cut(s) 210
BtuMI TCGCGA 2 cut(s) 189, 399
Cac8I GCNNGC 2 cut(s) 251, 378
CfoI GCGC 2 cut(s) 251, 253
Cfr13I GGNCC 1 cut(s) 5
CseI GACGC 2 cut(s) 106, 116
CsiI ACCWGGT 1 cut(s) 168
CviAII CATG 1 cut(s) 148
CviJI RGCY 9 cut(s) 12, 56, 67, 182, 203, 296, 310, 380, 478
CviKI_1 RGCY 9 cut(s) 12, 56, 67, 182, 203, 296, 310, 380, 478
DdeI CTNAG 1 cut(s) 213
DpnI GATC 2 cut(s) 159, 336
DpnII GATC 2 cut(s) 157, 334
Eam1104I CTCTTC 1 cut(s) 546
EarI CTCTTC 1 cut(s) 546
Eco130I CCWWGG 1 cut(s) 31
Eco147I AGGCCT 1 cut(s) 203
Eco31I GGTCTC 1 cut(s) 185
Eco47I GGWCC 1 cut(s) 5
Eco88I CYCGRG 1 cut(s) 80
EcoRII CCWGG 3 cut(s) 7, 168, 364
EcoT14I CCWWGG 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 31
Esp3I CGTCTC 1 cut(s) 89
FaeI CATG 1 cut(s) 151
FaiI YATR 6 cut(s) 47, 126, 149, 313, 345, 411
FaqI GGGAC 2 cut(s) 53, 504
FatI CATG 1 cut(s) 147
FbaI TGATCA 1 cut(s) 334
FblI GTMKAC 2 cut(s) 129, 174
FokI GGATG 2 cut(s) 48, 463
FspBI CTAG 1 cut(s) 228
GlaI GCGC 2 cut(s) 250, 252
GsaI CCCAGC 1 cut(s) 384
HaeIII GGCC 1 cut(s) 203
HapII CCGG 2 cut(s) 92, 262
HgaI GACGC 2 cut(s) 106, 116
HhaI GCGC 2 cut(s) 251, 253
Hin1I GRCGYC 2 cut(s) 98, 108
Hin1II CATG 1 cut(s) 151
Hin6I GCGC 2 cut(s) 249, 251
HinP1I GCGC 2 cut(s) 249, 251
HinfI GANTC 1 cut(s) 75
HpaII CCGG 2 cut(s) 92, 262
HphI GGTGA 2 cut(s) 61, 430
Hpy166II GTNNAC 2 cut(s) 130, 175
Hpy188I TCNGA 2 cut(s) 275, 300
Hpy188III TCNNGA 3 cut(s) 188, 332, 398
Hpy8I GTNNAC 2 cut(s) 130, 175
Hpy99I CGWCG 3 cut(s) 110, 158, 404
HpyAV CCTTC 4 cut(s) 270, 409, 415, 464
HpyCH4III ACNGT 2 cut(s) 139, 495
HpyCH4V TGCA 1 cut(s) 144
HpyF10VI GCNNNNNNNGC 3 cut(s) 97, 150, 250
HpyF3I CTNAG 1 cut(s) 213
Hsp92I GRCGYC 2 cut(s) 98, 108
Hsp92II CATG 1 cut(s) 151
HspAI GCGC 2 cut(s) 249, 251
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 2 cut(s) 157, 334
LmnI GCTCC 2 cut(s) 17, 264
LweI GCATC 1 cut(s) 291
MabI ACCWGGT 1 cut(s) 168
MaeI CTAG 1 cut(s) 228
MalI GATC 2 cut(s) 159, 336
MboI GATC 2 cut(s) 157, 334
MboII GAAGA 1 cut(s) 563
MseI TTAA 4 cut(s) 63, 372, 456, 522
MslI CAYNNNNRTG 1 cut(s) 123
MspI CCGG 2 cut(s) 92, 262
MspR9I CCNGG 4 cut(s) 9, 170, 263, 366
MvaI CCWGG 3 cut(s) 9, 170, 366
MvnI CGCG 3 cut(s) 189, 251, 399
MwoI GCNNNNNNNGC 3 cut(s) 97, 150, 250
NciI CCSGG 1 cut(s) 263
NdeII GATC 2 cut(s) 157, 334
NlaIII CATG 1 cut(s) 151
NlaIV GGNNCC 3 cut(s) 6, 13, 363
NruI TCGCGA 2 cut(s) 189, 399
NspI RCATGY 1 cut(s) 151
PaeR7I CTCGAG 1 cut(s) 80
PauI GCGCGC 1 cut(s) 249
PceI AGGCCT 1 cut(s) 203
PciI ACATGT 1 cut(s) 147
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PfeI GAWTC 1 cut(s) 75
PfoI TCCNGGA 1 cut(s) 261
PscI ACATGT 1 cut(s) 147
PshBI ATTAAT 1 cut(s) 456
Psp6I CCWGG 3 cut(s) 7, 168, 364
PspFI CCCAGC 1 cut(s) 380
PspGI CCWGG 3 cut(s) 7, 168, 364
PspN4I GGNNCC 3 cut(s) 6, 13, 363
PspPI GGNCC 1 cut(s) 5
PspXI VCTCGAGB 1 cut(s) 80
PteI GCGCGC 1 cut(s) 249
RruI TCGCGA 2 cut(s) 189, 399
RseI CAYNNNNRTG 1 cut(s) 123
SaqAI TTAA 4 cut(s) 63, 372, 456, 522
Sau3AI GATC 2 cut(s) 157, 334
Sau96I GGNCC 1 cut(s) 5
ScrFI CCNGG 4 cut(s) 9, 170, 263, 366
SetI ASST 6 cut(s) 69, 174, 281, 363, 408, 420
SexAI ACCWGGT 1 cut(s) 168
SfaNI GCATC 1 cut(s) 291
Sfr274I CTCGAG 1 cut(s) 80
SinI GGWCC 1 cut(s) 5
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 1 cut(s) 123
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
SseBI AGGCCT 1 cut(s) 203
SsiI CCGC 2 cut(s) 304, 542
SspMI CTAG 1 cut(s) 228
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 4 cut(s) 7, 168, 261, 364
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 2 cut(s) 139, 495
TaqI TCGA 5 cut(s) 21, 27, 81, 105, 198
TfiI GAWTC 1 cut(s) 75
Tru1I TTAA 4 cut(s) 63, 372, 456, 522
Tru9I TTAA 4 cut(s) 63, 372, 456, 522
TscAI CASTG 1 cut(s) 217
TspRI CASTG 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 5
VspI ATTAAT 1 cut(s) 456
XceI RCATGY 1 cut(s) 151
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 2 cut(s) 129, 174
XspI CTAG 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.