RchiOBHm_Chr5g0050191

Alkaline ceramidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
N/A
Physical Location & Seq
Reverse (-)
48716502 .. 48716909
408 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 408 bp
ATGCACCTAGACATGTATATCCTCTACTCCCCAGATTGGCACTATTGCAGTACAATGCCTACCTTCCTCTTCCTATATGGTGTTGTTTTTGCTGCAATTCATTCAGTGGTCCGATTTGAGATTGGTTTTGAGGTGCACTATGTGATCTTGTGTCTTTTTTACATACCTCGAATGTACAAGTATTATATATACACACAGGACGTCTATGCCAAGAGGCTCGCAAAGTTGTATGTGGCCACTCTTCTTAGAGGGAGTTTGTGTTGGCTCAATGACAATGTTTTCTGCATAGAGATATCGAGTTGGCCCATTAACCCGCAAGGTCATGCCTTATGGCATTTATTCATGGGTTTTAATTCCTATTTTGTAAACACTTTCTTGATGTTTTGTCGAGCTGAACAACGAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001101 GO:0002238 GO:0002376 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0006066 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006671 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0008150 GO:0008152 GO:0008284 GO:0008610 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009814 GO:0009987 GO:0010025 GO:0010033 GO:0010119 GO:0010166 GO:0012505 GO:0016020 GO:0016021 GO:0016787 GO:0016798 GO:0016810 GO:0016811 GO:0017040 GO:0019751 GO:0030104 GO:0030148 GO:0030173 GO:0030176 GO:0031090 GO:0031224 GO:0031227 GO:0031228 GO:0031300 GO:0031301 GO:0031984 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0042127 GO:0042175 GO:0042221 GO:0042592 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046165 GO:0046173 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0048518 GO:0048522 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0065007 GO:0065008 GO:0070774 GO:0071602 GO:0071704 GO:0090333 GO:0097164 GO:0097305 GO:0098542 GO:0098588 GO:0098791 GO:0098827 GO:1901564 GO:1901566 GO:1901568 GO:1901570 GO:1901576 GO:1901615 GO:1901617 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00600 map00600
Pfam Domains
Protein Families
Ceramidase

Protein Analysis

135

Amino Acids

16.18

Weight (kDa)

7.67

Isoelectric Point (pI)

59.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase PF05875 5 - 134 3.6e-20 Ceramidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 204
AciI CCGC 1 cut(s) 314
AcoI YGGCCR 1 cut(s) 234
AcyI GRCGYC 1 cut(s) 201
AdeI CACNNNGTG 1 cut(s) 142
AfaI GTAC 2 cut(s) 52, 176
AfiI CCNNNNNNNGG 1 cut(s) 36
AflIII ACRYGT 1 cut(s) 12
AluBI AGCT 1 cut(s) 392
AluI AGCT 1 cut(s) 392
Alw21I GWGCWC 1 cut(s) 138
Alw44I GTGCAC 1 cut(s) 134
AoxI GGCC 2 cut(s) 234, 302
ApaLI GTGCAC 1 cut(s) 134
ApeKI GCWGC 1 cut(s) 92
AspS9I GGNCC 2 cut(s) 109, 303
AvaII GGWCC 1 cut(s) 109
BaeGI GKGCMC 1 cut(s) 138
BalI TGGCCA 1 cut(s) 236
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 1 cut(s) 79
BfaI CTAG 1 cut(s) 8
BisI GCNGC 1 cut(s) 93
BlsI GCNGC 1 cut(s) 94
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 2 cut(s) 109, 303
BsaHI GRCGYC 1 cut(s) 201
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bsc4I CCNNNNNNNGG 1 cut(s) 36
BseLI CCNNNNNNNGG 1 cut(s) 36
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 1 cut(s) 79
BshFI GGCC 2 cut(s) 236, 304
BsiHKAI GWGCWC 1 cut(s) 138
BslI CCNNNNNNNGG 1 cut(s) 36
BsnI GGCC 2 cut(s) 236, 304
Bsp1286I GDGCHC 1 cut(s) 138
Bsp1407I TGTACA 1 cut(s) 174
Bsp143I GATC 1 cut(s) 144
BspACI CCGC 1 cut(s) 314
BspANI GGCC 2 cut(s) 236, 304
BsrGI TGTACA 1 cut(s) 174
BssMI GATC 1 cut(s) 144
BssNI GRCGYC 1 cut(s) 201
Bst6I CTCTTC 2 cut(s) 74, 246
BstACI GRCGYC 1 cut(s) 201
BstAUI TGTACA 1 cut(s) 174
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 1 cut(s) 245
BstKTI GATC 1 cut(s) 147
BstMBI GATC 1 cut(s) 144
BstNSI RCATGY 1 cut(s) 16
BstSLI GKGCMC 1 cut(s) 138
BstV1I GCAGC 1 cut(s) 79
BsuRI GGCC 2 cut(s) 236, 304
BtsIMutI CAGTG 1 cut(s) 111
Cac8I GCNNGC 1 cut(s) 219
Cfr13I GGNCC 2 cut(s) 109, 303
Csp6I GTAC 2 cut(s) 51, 175
CviAII CATG 3 cut(s) 13, 323, 343
CviJI RGCY 5 cut(s) 217, 236, 265, 304, 392
CviKI_1 RGCY 5 cut(s) 217, 236, 265, 304, 392
CviQI GTAC 2 cut(s) 51, 175
DdeI CTNAG 1 cut(s) 245
DpnI GATC 1 cut(s) 146
DpnII GATC 1 cut(s) 144
DraIII CACNNNGTG 1 cut(s) 142
EaeI YGGCCR 1 cut(s) 234
Eam1104I CTCTTC 2 cut(s) 74, 246
EarI CTCTTC 2 cut(s) 74, 246
Eco32I GATATC 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 109
EcoRV GATATC 1 cut(s) 294
FaeI CATG 3 cut(s) 16, 326, 346
FatI CATG 3 cut(s) 12, 322, 342
FauI CCCGC 1 cut(s) 321
Fnu4HI GCNGC 1 cut(s) 93
Fsp4HI GCNGC 1 cut(s) 93
FspBI CTAG 1 cut(s) 8
GluI GCNGC 1 cut(s) 93
HaeIII GGCC 2 cut(s) 236, 304
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 3 cut(s) 16, 326, 346
Hpy166II GTNNAC 2 cut(s) 136, 367
Hpy188I TCNGA 1 cut(s) 113
Hpy188III TCNNGA 1 cut(s) 376
Hpy8I GTNNAC 2 cut(s) 136, 367
HpyAV CCTTC 1 cut(s) 73
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 5 cut(s) 4, 48, 95, 136, 285
HpyF3I CTNAG 1 cut(s) 245
HpySE526I ACGT 1 cut(s) 201
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 3 cut(s) 16, 326, 346
Kzo9I GATC 1 cut(s) 144
LpnPI CCDG 2 cut(s) 45, 182
Lsp1109I GCAGC 1 cut(s) 79
MaeI CTAG 1 cut(s) 8
MaeII ACGT 1 cut(s) 201
MalI GATC 1 cut(s) 146
MboI GATC 1 cut(s) 144
MboII GAAGA 2 cut(s) 61, 233
MhlI GDGCHC 1 cut(s) 138
MlsI TGGCCA 1 cut(s) 236
MluCI AATT 2 cut(s) 96, 352
MluNI TGGCCA 1 cut(s) 236
MnlI CCTC 7 cut(s) 32, 77, 124, 177, 207, 242, 395
Mox20I TGGCCA 1 cut(s) 236
MscI TGGCCA 1 cut(s) 236
MseI TTAA 2 cut(s) 309, 351
Msp20I TGGCCA 1 cut(s) 236
NdeII GATC 1 cut(s) 144
NlaIII CATG 3 cut(s) 16, 326, 346
NspI RCATGY 1 cut(s) 16
PciI ACATGT 1 cut(s) 12
PkrI GCNGC 1 cut(s) 94
PscI ACATGT 1 cut(s) 12
PspPI GGNCC 2 cut(s) 109, 303
RsaI GTAC 2 cut(s) 52, 176
RsaNI GTAC 2 cut(s) 51, 175
SaqAI TTAA 2 cut(s) 309, 351
SatI GCNGC 1 cut(s) 93
Sau3AI GATC 1 cut(s) 144
Sau96I GGNCC 2 cut(s) 109, 303
SduI GDGCHC 1 cut(s) 138
SetI ASST 7 cut(s) 9, 65, 135, 169, 204, 322, 394
SinI GGWCC 1 cut(s) 109
Sse9I AATT 2 cut(s) 96, 352
SsiI CCGC 1 cut(s) 314
SspMI CTAG 1 cut(s) 8
TaiI ACGT 1 cut(s) 204
TaqI TCGA 3 cut(s) 169, 296, 388
TasI AATT 2 cut(s) 96, 352
TatI WGTACW 2 cut(s) 50, 174
Tru1I TTAA 2 cut(s) 309, 351
Tru9I TTAA 2 cut(s) 309, 351
TscAI CASTG 1 cut(s) 111
TseI GCWGC 1 cut(s) 92
TspDTI ATGAA 2 cut(s) 89, 331
TspRI CASTG 1 cut(s) 111
VneI GTGCAC 1 cut(s) 134
VpaK11BI GGWCC 1 cut(s) 109
XceI RCATGY 1 cut(s) 16
XspI CTAG 1 cut(s) 8
ZraI GACGTC 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.