RchiOBHm_Chr5g0063121

histone H2B.1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
N/A
Physical Location & Seq
Forward (+)
68870342 .. 68873965
3624 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
ATGGATCTCTCTCCCCCATATATTAAAAACCACCCCACACGTCTCCAAATCCCACACCAACCCCTCTCGCTCCCAATCGGTCTCTACTCTCTTAATTTCCCAAATTCCCGAACCCCAACTTTCTGTCTCCAACCCCAAATGGCACCCAAGGCCGAGAAGAAGCCCGCCGAGAAGAAGCCAGCAGAGGAGAAGAAGTCCACCGTCGCCGAGAAGGCCCCCGCCGAGAAGAAGCCCAAGGCCGGCAAGAAGCTCCCCAAGGAGGCCGGAGCTGCCGCCGGAGACAAGAAGAAGAAGAGGAACAAGAAGAGCGTAGAGACCTACAAGATCTACATCTTCAAGGTCCTCAAGCAGGTCCACCCTGACATCGGGATCTCCAGCAAGGCCATGGGCATCATGAACAGCTTCATCAACGATATCTTTGAGAAGCTCGCTCAGGAGTCGTCGAGGCTTGCGAGGTACAACAAGAAGCCGACGATTACTTCTCGGGAGATCCAGACTGCTGTCAGGCTTGTGCTTCCCGGTGAATTGGCTAAGCACGCTGTTTCTGAGGGTACAAAGGCTGTGACCAAGTTCACGAGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

194

Amino Acids

21.5

Weight (kDa)

10.05

Isoelectric Point (pI)

37.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 94 - 170 4.7e-20 Core histone H2A/H2B/H3/H4 domain
Histone_ABTB PF26281 124 - 192 9.2e-08 Histone domain in ABTB family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 340
AccB1I GGYRCC 1 cut(s) 142
AciI CCGC 3 cut(s) 165, 219, 273
AclWI GGATC 3 cut(s) 12, 377, 484
AcsI RAATTY 1 cut(s) 103
AfaI GTAC 2 cut(s) 458, 553
AfiI CCNNNNNNNGG 4 cut(s) 239, 259, 349, 365
AflIII ACRYGT 1 cut(s) 38
AgsI TTSAA 1 cut(s) 337
AjiI CACGTC 1 cut(s) 41
AluBI AGCT 4 cut(s) 250, 269, 402, 427
AluI AGCT 4 cut(s) 250, 269, 402, 427
Alw26I GTCTC 5 cut(s) 47, 86, 131, 273, 308
AlwI GGATC 3 cut(s) 12, 377, 484
Ama87I CYCGRG 1 cut(s) 483
AoxI GGCC 5 cut(s) 150, 213, 237, 261, 381
ApeKI GCWGC 1 cut(s) 269
ApoI RAATTY 1 cut(s) 103
Asp700I GAANNNNTTC 1 cut(s) 401
AspS9I GGNCC 3 cut(s) 214, 340, 352
AsuC2I CCSGG 1 cut(s) 519
AsuHPI GGTGA 1 cut(s) 533
AvaI CYCGRG 1 cut(s) 483
AvaII GGWCC 2 cut(s) 340, 352
BanI GGYRCC 1 cut(s) 142
BauI CACGAG 1 cut(s) 574
BbvI GCAGC 1 cut(s) 256
BcnI CCSGG 1 cut(s) 519
BcoDI GTCTC 5 cut(s) 47, 86, 131, 273, 308
BfuAI ACCTGC 1 cut(s) 340
BglI GCCNNNNNGGC 1 cut(s) 212
BglII AGATCT 1 cut(s) 324
BisI GCNGC 2 cut(s) 270, 273
BlpI GCTNAGC 1 cut(s) 531
BlsI GCNGC 2 cut(s) 271, 274
Bme1390I CCNGG 1 cut(s) 519
Bme18I GGWCC 2 cut(s) 340, 352
BmeT110I CYCGRG 1 cut(s) 483
BmgBI CACGTC 1 cut(s) 41
BmgT120I GGNCC 3 cut(s) 214, 340, 352
BmiI GGNNCC 2 cut(s) 144, 216
BmrFI CCNGG 1 cut(s) 519
BmsI GCATC 1 cut(s) 399
BoxI GACNNNNGTC 1 cut(s) 500
BpmI CTGGAG 1 cut(s) 358
Bpu10I CCTNAGC 1 cut(s) 432
Bpu1102I GCTNAGC 1 cut(s) 531
BpuEI CTTGAG 1 cut(s) 329
BpuMI CCSGG 1 cut(s) 519
BsaBI GATNNNNATC 1 cut(s) 329
BsaI GGTCTC 2 cut(s) 86, 308
BsaJI CCNNGG 4 cut(s) 147, 234, 255, 384
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bsc4I CCNNNNNNNGG 4 cut(s) 239, 259, 349, 365
Bse118I RCCGGY 1 cut(s) 239
Bse8I GATNNNNATC 1 cut(s) 329
BseDI CCNNGG 4 cut(s) 147, 234, 255, 384
BseJI GATNNNNATC 1 cut(s) 329
BseLI CCNNNNNNNGG 4 cut(s) 239, 259, 349, 365
BseMII CTCAG 2 cut(s) 446, 537
BseRI GAGGAG 1 cut(s) 200
BseXI GCAGC 1 cut(s) 256
BshFI GGCC 5 cut(s) 152, 215, 239, 263, 383
BshNI GGYRCC 1 cut(s) 142
BsiHKCI CYCGRG 1 cut(s) 483
BsiSI CCGG 4 cut(s) 240, 264, 276, 519
BslI CCNNNNNNNGG 4 cut(s) 239, 259, 349, 365
BsmAI GTCTC 5 cut(s) 47, 86, 131, 273, 308
BsmBI CGTCTC 1 cut(s) 47
BsnI GGCC 5 cut(s) 152, 215, 239, 263, 383
Bso31I GGTCTC 2 cut(s) 86, 308
BsoBI CYCGRG 1 cut(s) 483
Bsp143I GATC 4 cut(s) 4, 324, 369, 489
Bsp1720I GCTNAGC 1 cut(s) 531
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 3 cut(s) 165, 219, 273
BspANI GGCC 5 cut(s) 152, 215, 239, 263, 383
BspCNI CTCAG 2 cut(s) 445, 538
BspHI TCATGA 1 cut(s) 393
BspLI GGNNCC 2 cut(s) 144, 216
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 3 cut(s) 12, 377, 484
BspQI GCTCTTC 1 cut(s) 299
BspT107I GGYRCC 1 cut(s) 142
BspTNI GGTCTC 2 cut(s) 86, 308
BsrFI RCCGGY 1 cut(s) 239
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 4 cut(s) 147, 234, 255, 384
BssMI GATC 4 cut(s) 4, 324, 369, 489
BssSI CACGAG 1 cut(s) 574
BssT1I CCWWGG 4 cut(s) 147, 234, 255, 384
Bst2BI CACGAG 1 cut(s) 574
Bst4CI ACNGT 1 cut(s) 202
Bst6I CTCTTC 2 cut(s) 287, 299
BstC8I GCNNGC 6 cut(s) 165, 180, 241, 429, 450, 537
BstDEI CTNAG 3 cut(s) 432, 531, 546
BstDSI CCRYGG 1 cut(s) 384
BstENI CCTNNNNNAGG 1 cut(s) 347
BstKTI GATC 4 cut(s) 7, 327, 372, 492
BstMAI GTCTC 5 cut(s) 47, 86, 131, 273, 308
BstMBI GATC 4 cut(s) 4, 324, 369, 489
BstMWI GCNNNNNNNGC 4 cut(s) 149, 212, 269, 536
BstPAI GACNNNNGTC 1 cut(s) 500
BstSCI CCNGG 1 cut(s) 517
BstV1I GCAGC 1 cut(s) 256
BstX2I RGATCY 4 cut(s) 4, 324, 369, 489
BstYI RGATCY 4 cut(s) 4, 324, 369, 489
BsuRI GGCC 5 cut(s) 152, 215, 239, 263, 383
BtgI CCRYGG 1 cut(s) 384
BtrI CACGTC 1 cut(s) 41
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 6 cut(s) 165, 180, 241, 429, 450, 537
CciI TCATGA 1 cut(s) 393
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 3 cut(s) 214, 340, 352
Csp6I GTAC 2 cut(s) 457, 552
CviAII CATG 2 cut(s) 385, 394
CviQI GTAC 2 cut(s) 457, 552
DdeI CTNAG 3 cut(s) 432, 531, 546
DpnI GATC 4 cut(s) 6, 326, 371, 491
DpnII GATC 4 cut(s) 4, 324, 369, 489
Eam1104I CTCTTC 2 cut(s) 287, 299
EarI CTCTTC 2 cut(s) 287, 299
Eco130I CCWWGG 4 cut(s) 147, 234, 255, 384
Eco31I GGTCTC 2 cut(s) 86, 308
Eco32I GATATC 1 cut(s) 415
Eco47I GGWCC 2 cut(s) 340, 352
Eco88I CYCGRG 1 cut(s) 483
EcoNI CCTNNNNNAGG 1 cut(s) 347
EcoO109I RGGNCCY 2 cut(s) 214, 340
EcoRV GATATC 1 cut(s) 415
EcoT14I CCWWGG 4 cut(s) 147, 234, 255, 384
ErhI CCWWGG 4 cut(s) 147, 234, 255, 384
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 2 cut(s) 388, 397
FaiI YATR 4 cut(s) 19, 21, 386, 395
FatI CATG 2 cut(s) 384, 393
FauI CCCGC 2 cut(s) 172, 226
Fnu4HI GCNGC 2 cut(s) 270, 273
Fsp4HI GCNGC 2 cut(s) 270, 273
GluI GCNGC 2 cut(s) 270, 273
GsuI CTGGAG 1 cut(s) 358
HaeIII GGCC 5 cut(s) 152, 215, 239, 263, 383
HapII CCGG 4 cut(s) 240, 264, 276, 519
Hin1II CATG 2 cut(s) 388, 397
HinfI GANTC 1 cut(s) 437
HpaII CCGG 4 cut(s) 240, 264, 276, 519
HphI GGTGA 1 cut(s) 533
Hpy166II GTNNAC 3 cut(s) 198, 355, 573
Hpy188I TCNGA 1 cut(s) 547
Hpy188III TCNNGA 8 cut(s) 108, 367, 394, 434, 485, 493, 574, 582
Hpy8I GTNNAC 3 cut(s) 198, 355, 573
Hpy99I CGWCG 3 cut(s) 206, 445, 475
HpyAV CCTTC 1 cut(s) 205
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4IV ACGT 1 cut(s) 40
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 212, 269, 536
HpyF3I CTNAG 3 cut(s) 432, 531, 546
HpySE526I ACGT 1 cut(s) 40
Hsp92II CATG 2 cut(s) 388, 397
KroI GCCGGC 1 cut(s) 239
KroNI GCCGGC 1 cut(s) 241
Kzo9I GATC 4 cut(s) 4, 324, 369, 489
LguI GCTCTTC 1 cut(s) 299
LmnI GCTCC 3 cut(s) 75, 255, 266
Lsp1109I GCAGC 1 cut(s) 256
LweI GCATC 1 cut(s) 399
MaeII ACGT 1 cut(s) 40
MaeIII GTNAC 1 cut(s) 562
MalI GATC 4 cut(s) 6, 326, 371, 491
MboI GATC 4 cut(s) 4, 324, 369, 489
MboII GAAGA 9 cut(s) 169, 184, 202, 238, 298, 301, 304, 316, 325
MflI RGATCY 4 cut(s) 4, 324, 369, 489
MluCI AATT 3 cut(s) 94, 103, 524
MlyI GAGTC 1 cut(s) 446
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 8 cut(s) 74, 178, 253, 288, 353, 438, 447, 541
MroNI GCCGGC 1 cut(s) 239
MroXI GAANNNNTTC 1 cut(s) 401
MseI TTAA 2 cut(s) 24, 93
MspI CCGG 4 cut(s) 240, 264, 276, 519
MspR9I CCNGG 1 cut(s) 519
MwoI GCNNNNNNNGC 4 cut(s) 149, 212, 269, 536
NaeI GCCGGC 1 cut(s) 241
NciI CCSGG 1 cut(s) 519
NcoI CCATGG 1 cut(s) 384
NdeII GATC 4 cut(s) 4, 324, 369, 489
NgoMIV GCCGGC 1 cut(s) 239
NlaIII CATG 2 cut(s) 388, 397
NlaIV GGNNCC 2 cut(s) 144, 216
NmeAIII GCCGAG 4 cut(s) 178, 193, 232, 247
NmuCI GTSAC 1 cut(s) 562
PagI TCATGA 1 cut(s) 393
PciSI GCTCTTC 1 cut(s) 299
PcsI WCGNNNNNNNCGW 1 cut(s) 449
PdiI GCCGGC 1 cut(s) 241
PdmI GAANNNNTTC 1 cut(s) 401
PkrI GCNGC 2 cut(s) 271, 274
PleI GAGTC 1 cut(s) 445
PpsI GAGTC 1 cut(s) 445
PpuMI RGGWCCY 1 cut(s) 340
PshAI GACNNNNGTC 1 cut(s) 500
Psp5II RGGWCCY 1 cut(s) 340
PspN4I GGNNCC 2 cut(s) 144, 216
PspPI GGNCC 3 cut(s) 214, 340, 352
PspPPI RGGWCCY 1 cut(s) 340
PsuI RGATCY 4 cut(s) 4, 324, 369, 489
RsaI GTAC 2 cut(s) 458, 553
RsaNI GTAC 2 cut(s) 457, 552
SapI GCTCTTC 1 cut(s) 299
SaqAI TTAA 2 cut(s) 24, 93
SatI GCNGC 2 cut(s) 270, 273
Sau3AI GATC 4 cut(s) 4, 324, 369, 489
Sau96I GGNCC 3 cut(s) 214, 340, 352
SchI GAGTC 1 cut(s) 446
ScrFI CCNGG 1 cut(s) 519
SetI ASST 9 cut(s) 43, 252, 271, 320, 342, 354, 404, 429, 458
SfaNI GCATC 1 cut(s) 399
SinI GGWCC 2 cut(s) 340, 352
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 3 cut(s) 94, 103, 524
SsiI CCGC 3 cut(s) 165, 219, 273
StyD4I CCNGG 1 cut(s) 517
StyI CCWWGG 4 cut(s) 147, 234, 255, 384
TaaI ACNGT 1 cut(s) 202
TaiI ACGT 1 cut(s) 43
TaqI TCGA 1 cut(s) 443
TaqII GACCGA 1 cut(s) 68
TasI AATT 3 cut(s) 94, 103, 524
TauI GCSGC 1 cut(s) 275
Tru1I TTAA 2 cut(s) 24, 93
Tru9I TTAA 2 cut(s) 24, 93
TseFI GTSAC 1 cut(s) 562
TseI GCWGC 1 cut(s) 269
Tsp45I GTSAC 1 cut(s) 562
TspDTI ATGAA 2 cut(s) 394, 410
VpaK11BI GGWCC 2 cut(s) 340, 352
XagI CCTNNNNNAGG 1 cut(s) 347
XapI RAATTY 1 cut(s) 103
XcmI CCANNNNNNNNNTGG 1 cut(s) 382
XmnI GAANNNNTTC 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.