RchiOBHm_Chr5g0073421

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
N/A
Physical Location & Seq
Reverse (-)
79455165 .. 79458106
2942 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1026 bp
ATGGTGGCGACATGGAAAGAAAGCGTGACAAGCTCCATCAACACCATCTATCTCCTCTTCTCTGCCTATCTTGTCTTCCTCATGCAGCTCGGCTTTGCCAAGCTCCGTGCTGGCTCTGTTCTATCCAAGAATGCCACAAACATAATGCTCTCCAATGTAGTGGATGCCATTGTAGGTAGCCTCTCTTTCGGTATCTTTGGATTCGCTTTTGCATTTGGAGAAGGCTCCAATTCCAACGCTTTCATTGCTGAGAGAACCCAATTCAATGCATACCTCATTTTCTCTTGTTTTCTGTCTGGGTTTGTGTACCCTGTGGTGGCTCACTGGGTTTGGTCATCAAGTGCCCGCACCAATCCGAGGCCACAAAACTACCCTCGTGGTGCTCGGGATTTGTGGTTCGGATGGCTTGGGTTTAATCCGGGCTCATTCGATAAGATTCTCGTGGCCTATCCAGACACCATTGATGAAGGGAATTGGACTGCAGTGGGCCGATCGGTTGTTGTGACACCGTTAGCCGAGTCAACTTCTGGAATCGTAACCCTGTACGTAGTTCCGCCCCGTGCACTAGATGTTTGCAATGGAGTGCTTGGTGGGTTTGTTGCAATAACTTCCAGATGTGCGGTGGTTGAGCCATGTGCCGCAGTTGTTTGTGGATTTTTCGCCGCTTGGGTCTTAATTGGGATGAATATCTTGGCCTTCAAGCTTCAATTTGACGACCCTTTAGAGGTGGCCCAATTACACGGTTGGTGTGGTGCTTGGGCTTGGCCTTATGGTCCATTAATGGGTGGGGTTGGGCTGCTCCGCGCCCAAGTGGTTGAGGCTTTGGTGATTTTATGCTGGGTTAGCTTGACAATGGGTCCTCTATTCTACACCCTTCACAAGCTTAACGTTCTGAGGATCTCAGTCGATGAAGAAATTACAGGTCTTGATGTCTGTAGCCATGGAGGCCATGTACATGTTAATACATACGAGATTCAGCCTCCCTTTTATGCAGAATATCTGCCTATGCGAGAAGATGGGTCATGA

Protein Analysis

341

Amino Acids

37.24

Weight (kDa)

5.49

Isoelectric Point (pI)

23.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 18 - 82 3.5e-12 Ammonium Transporter Family
Ammonium_transp PF00909 81 - 112 3.8e-08 Ammonium Transporter Family
Ammonium_transp PF00909 130 - 316 1e-35 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 804
AciI CCGC 6 cut(s) 346, 554, 620, 639, 663, 802
AclI AACGTT 1 cut(s) 888
AclWI GGATC 1 cut(s) 905
AfaI GTAC 3 cut(s) 308, 545, 954
AfiI CCNNNNNNNGG 4 cut(s) 316, 357, 724, 782
AflIII ACRYGT 1 cut(s) 955
AgsI TTSAA 3 cut(s) 265, 700, 707
AhdI GACNNNNNGTC 1 cut(s) 855
AloI GAACNNNNNNTCC 2 cut(s) 380, 412
AluBI AGCT 6 cut(s) 33, 88, 103, 703, 846, 883
AluI AGCT 6 cut(s) 33, 88, 103, 703, 846, 883
Alw21I GWGCWC 2 cut(s) 385, 565
Alw44I GTGCAC 1 cut(s) 561
AlwI GGATC 1 cut(s) 905
Ama87I CYCGRG 1 cut(s) 384
AoxI GGCC 7 cut(s) 359, 444, 487, 693, 729, 764, 946
ApaLI GTGCAC 1 cut(s) 561
ApeKI GCWGC 2 cut(s) 85, 796
AseI ATTAAT 1 cut(s) 779
AspLEI GCGC 1 cut(s) 806
AspS9I GGNCC 4 cut(s) 487, 730, 773, 857
AsuC2I CCSGG 1 cut(s) 420
AsuHPI GGTGA 1 cut(s) 838
AvaI CYCGRG 1 cut(s) 384
AvaII GGWCC 2 cut(s) 773, 857
BaeGI GKGCMC 2 cut(s) 346, 565
BanII GRGCYC 1 cut(s) 425
BauI CACGAG 2 cut(s) 375, 440
BbsI GAAGAC 1 cut(s) 67
Bbv12I GWGCWC 2 cut(s) 385, 565
BbvI GCAGC 2 cut(s) 97, 783
BccI CCATC 4 cut(s) 44, 53, 396, 1010
BcnI CCSGG 1 cut(s) 420
BfaI CTAG 1 cut(s) 566
BfmI CTRYAG 2 cut(s) 480, 934
BglI GCCNNNNNGGC 1 cut(s) 945
BisI GCNGC 4 cut(s) 86, 639, 663, 797
BlsI GCNGC 4 cut(s) 87, 640, 664, 798
Bme1390I CCNGG 1 cut(s) 420
Bme18I GGWCC 2 cut(s) 773, 857
BmeRI GACNNNNNGTC 1 cut(s) 855
BmeT110I CYCGRG 1 cut(s) 384
BmgT120I GGNCC 4 cut(s) 487, 730, 773, 857
BmiI GGNNCC 2 cut(s) 226, 858
BmrFI CCNGG 1 cut(s) 420
BmrI ACTGGG 1 cut(s) 334
BmsI GCATC 1 cut(s) 154
BmuI ACTGGG 1 cut(s) 334
BpiI GAAGAC 1 cut(s) 67
BpuMI CCSGG 1 cut(s) 420
BsaAI YACGTR 1 cut(s) 547
BsaBI GATNNNNATC 1 cut(s) 686
BsaJI CCNNGG 2 cut(s) 356, 940
BsaXI ACNNNNNCTCC 2 cut(s) 936, 966
Bsc4I CCNNNNNNNGG 4 cut(s) 316, 357, 724, 782
Bse1I ACTGG 1 cut(s) 329
Bse3DI GCAATG 2 cut(s) 243, 583
Bse8I GATNNNNATC 1 cut(s) 686
BseDI CCNNGG 2 cut(s) 356, 940
BseGI GGATG 3 cut(s) 169, 407, 687
BseJI GATNNNNATC 1 cut(s) 686
BseLI CCNNNNNNNGG 4 cut(s) 316, 357, 724, 782
BseMI GCAATG 2 cut(s) 243, 583
BseMII CTCAG 3 cut(s) 240, 884, 915
BseNI ACTGG 1 cut(s) 329
BseRI GAGGAG 1 cut(s) 44
BseSI GKGCMC 2 cut(s) 346, 565
BseXI GCAGC 2 cut(s) 97, 783
BseYI CCCAGC 1 cut(s) 837
Bsh1236I CGCG 1 cut(s) 804
Bsh1285I CGRYCG 1 cut(s) 494
BshFI GGCC 7 cut(s) 361, 446, 489, 695, 731, 766, 948
BsiEI CGRYCG 1 cut(s) 494
BsiHKAI GWGCWC 2 cut(s) 385, 565
BsiHKCI CYCGRG 1 cut(s) 384
BsiSI CCGG 1 cut(s) 419
BslI CCNNNNNNNGG 4 cut(s) 316, 357, 724, 782
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 7 cut(s) 361, 446, 489, 695, 731, 766, 948
BsoBI CYCGRG 1 cut(s) 384
Bsp1286I GDGCHC 4 cut(s) 346, 385, 425, 565
Bsp1407I TGTACA 1 cut(s) 952
Bsp143I GATC 2 cut(s) 491, 897
Bsp19I CCATGG 1 cut(s) 940
BspACI CCGC 6 cut(s) 346, 554, 620, 639, 663, 802
BspANI GGCC 7 cut(s) 361, 446, 489, 695, 731, 766, 948
BspCNI CTCAG 3 cut(s) 241, 885, 914
BspFNI CGCG 1 cut(s) 804
BspHI TCATGA 1 cut(s) 1022
BspLI GGNNCC 2 cut(s) 226, 858
BspMAI CTGCAG 1 cut(s) 484
BspPI GGATC 1 cut(s) 905
BsrDI GCAATG 2 cut(s) 243, 583
BsrGI TGTACA 1 cut(s) 952
BsrI ACTGG 1 cut(s) 329
BssECI CCNNGG 2 cut(s) 356, 940
BssMI GATC 2 cut(s) 491, 897
BssSI CACGAG 2 cut(s) 375, 440
BssT1I CCWWGG 1 cut(s) 940
Bst2BI CACGAG 2 cut(s) 375, 440
Bst4CI ACNGT 2 cut(s) 510, 743
Bst6I CTCTTC 1 cut(s) 62
BstAUI TGTACA 1 cut(s) 952
BstBAI YACGTR 1 cut(s) 547
BstC8I GCNNGC 2 cut(s) 112, 346
BstDEI CTNAG 3 cut(s) 249, 893, 901
BstDSI CCRYGG 1 cut(s) 940
BstF5I GGATG 3 cut(s) 169, 407, 687
BstFNI CGCG 1 cut(s) 804
BstHHI GCGC 1 cut(s) 806
BstKTI GATC 2 cut(s) 494, 900
BstMBI GATC 2 cut(s) 491, 897
BstMCI CGRYCG 1 cut(s) 494
BstMWI GCNNNNNNNGC 4 cut(s) 30, 245, 843, 945
BstNSI RCATGY 1 cut(s) 959
BstSCI CCNGG 1 cut(s) 418
BstSFI CTRYAG 2 cut(s) 480, 934
BstSLI GKGCMC 2 cut(s) 346, 565
BstSNI TACGTA 1 cut(s) 547
BstUI CGCG 1 cut(s) 804
BstV1I GCAGC 2 cut(s) 97, 783
BstV2I GAAGAC 1 cut(s) 67
BstX2I RGATCY 1 cut(s) 897
BstXI CCANNNNNNTGG 1 cut(s) 160
BstYI RGATCY 1 cut(s) 897
BsuRI GGCC 7 cut(s) 361, 446, 489, 695, 731, 766, 948
BtgI CCRYGG 1 cut(s) 940
BtsCI GGATG 3 cut(s) 169, 407, 687
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 2 cut(s) 322, 489
Cac8I GCNNGC 2 cut(s) 112, 346
CciI TCATGA 1 cut(s) 1022
CfoI GCGC 1 cut(s) 806
Cfr13I GGNCC 4 cut(s) 487, 730, 773, 857
Csp6I GTAC 3 cut(s) 307, 544, 953
CviAII CATG 7 cut(s) 12, 82, 633, 941, 950, 956, 1023
CviQI GTAC 3 cut(s) 307, 544, 953
DdeI CTNAG 3 cut(s) 249, 893, 901
DpnI GATC 2 cut(s) 493, 899
DpnII GATC 2 cut(s) 491, 897
DriI GACNNNNNGTC 1 cut(s) 855
Eam1104I CTCTTC 1 cut(s) 62
Eam1105I GACNNNNNGTC 1 cut(s) 855
EarI CTCTTC 1 cut(s) 62
EciI GGCGGA 1 cut(s) 543
Eco105I TACGTA 1 cut(s) 547
Eco130I CCWWGG 1 cut(s) 940
Eco24I GRGCYC 1 cut(s) 425
Eco47I GGWCC 2 cut(s) 773, 857
Eco88I CYCGRG 1 cut(s) 384
EcoO109I RGGNCCY 1 cut(s) 857
EcoT14I CCWWGG 1 cut(s) 940
EcoT22I ATGCAT 1 cut(s) 271
EcoT38I GRGCYC 1 cut(s) 425
ErhI CCWWGG 1 cut(s) 940
FaeI CATG 7 cut(s) 15, 85, 636, 944, 953, 959, 1026
FatI CATG 7 cut(s) 11, 81, 632, 940, 949, 955, 1022
FauI CCCGC 1 cut(s) 353
Fnu4HI GCNGC 4 cut(s) 86, 639, 663, 797
FokI GGATG 3 cut(s) 176, 414, 694
FriOI GRGCYC 1 cut(s) 425
Fsp4HI GCNGC 4 cut(s) 86, 639, 663, 797
FspBI CTAG 1 cut(s) 566
GlaI GCGC 1 cut(s) 805
GluI GCNGC 4 cut(s) 86, 639, 663, 797
GsaI CCCAGC 1 cut(s) 841
HaeIII GGCC 7 cut(s) 361, 446, 489, 695, 731, 766, 948
HapII CCGG 1 cut(s) 419
HhaI GCGC 1 cut(s) 806
Hin1II CATG 7 cut(s) 15, 85, 636, 944, 953, 959, 1026
Hin6I GCGC 1 cut(s) 804
HinP1I GCGC 1 cut(s) 804
HincII GTYRAC 1 cut(s) 522
HindII GTYRAC 1 cut(s) 522
HindIII AAGCTT 2 cut(s) 701, 881
HinfI GANTC 5 cut(s) 201, 436, 518, 531, 973
HpaII CCGG 1 cut(s) 419
HphI GGTGA 1 cut(s) 838
Hpy166II GTNNAC 3 cut(s) 307, 522, 563
Hpy188I TCNGA 3 cut(s) 357, 401, 894
Hpy188III TCNNGA 6 cut(s) 386, 452, 528, 612, 926, 1023
Hpy8I GTNNAC 3 cut(s) 307, 522, 563
HpyAV CCTTC 4 cut(s) 215, 461, 706, 884
HpyCH4III ACNGT 2 cut(s) 510, 743
HpyCH4IV ACGT 2 cut(s) 546, 888
HpyCH4V TGCA 8 cut(s) 85, 212, 269, 482, 563, 576, 602, 992
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 245, 843, 945
HpyF3I CTNAG 3 cut(s) 249, 893, 901
HpySE526I ACGT 2 cut(s) 546, 888
Hsp92II CATG 7 cut(s) 15, 85, 636, 944, 953, 959, 1026
HspAI GCGC 1 cut(s) 804
Kzo9I GATC 2 cut(s) 491, 897
LmnI GCTCC 4 cut(s) 38, 108, 230, 804
Lsp1109I GCAGC 2 cut(s) 97, 783
LweI GCATC 1 cut(s) 154
MaeI CTAG 1 cut(s) 566
MaeII ACGT 2 cut(s) 546, 888
MaeIII GTNAC 3 cut(s) 25, 502, 535
MalI GATC 2 cut(s) 493, 899
MboI GATC 2 cut(s) 491, 897
MboII GAAGA 4 cut(s) 49, 67, 923, 1025
MflI RGATCY 1 cut(s) 897
MhlI GDGCHC 4 cut(s) 346, 385, 425, 565
MluCI AATT 7 cut(s) 229, 260, 472, 675, 707, 734, 915
MlyI GAGTC 1 cut(s) 527
MmeI TCCRAC 1 cut(s) 258
Mph1103I ATGCAT 1 cut(s) 271
MseI TTAA 5 cut(s) 414, 674, 779, 885, 960
MslI CAYNNNNRTG 1 cut(s) 954
MspI CCGG 1 cut(s) 419
MspR9I CCNGG 1 cut(s) 420
Mva1269I GAATGC 1 cut(s) 136
MvnI CGCG 1 cut(s) 804
MwoI GCNNNNNNNGC 4 cut(s) 30, 245, 843, 945
NciI CCSGG 1 cut(s) 420
NcoI CCATGG 1 cut(s) 940
NdeII GATC 2 cut(s) 491, 897
NlaIII CATG 7 cut(s) 15, 85, 636, 944, 953, 959, 1026
NlaIV GGNNCC 2 cut(s) 226, 858
NmeAIII GCCGAG 2 cut(s) 69, 541
NmuCI GTSAC 2 cut(s) 25, 502
NsiI ATGCAT 1 cut(s) 271
NspI RCATGY 1 cut(s) 959
PagI TCATGA 1 cut(s) 1022
PciI ACATGT 1 cut(s) 955
PctI GAATGC 1 cut(s) 136
PfeI GAWTC 4 cut(s) 201, 436, 531, 973
PkrI GCNGC 4 cut(s) 87, 640, 664, 798
Ple19I CGATCG 1 cut(s) 494
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
Ppu21I YACGTR 1 cut(s) 547
PpuMI RGGWCCY 1 cut(s) 857
PscI ACATGT 1 cut(s) 955
PshBI ATTAAT 1 cut(s) 779
Psp1406I AACGTT 1 cut(s) 888
Psp5II RGGWCCY 1 cut(s) 857
PspFI CCCAGC 1 cut(s) 837
PspN4I GGNNCC 2 cut(s) 226, 858
PspPI GGNCC 4 cut(s) 487, 730, 773, 857
PspPPI RGGWCCY 1 cut(s) 857
PstI CTGCAG 1 cut(s) 484
PsuI RGATCY 1 cut(s) 897
PvuI CGATCG 1 cut(s) 494
RsaI GTAC 3 cut(s) 308, 545, 954
RsaNI GTAC 3 cut(s) 307, 544, 953
RseI CAYNNNNRTG 1 cut(s) 954
SaqAI TTAA 5 cut(s) 414, 674, 779, 885, 960
SatI GCNGC 4 cut(s) 86, 639, 663, 797
Sau3AI GATC 2 cut(s) 491, 897
Sau96I GGNCC 4 cut(s) 487, 730, 773, 857
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 1 cut(s) 420
SduI GDGCHC 4 cut(s) 346, 385, 425, 565
SfaNI GCATC 1 cut(s) 154
SfcI CTRYAG 2 cut(s) 480, 934
SinI GGWCC 2 cut(s) 773, 857
SmiMI CAYNNNNRTG 1 cut(s) 954
SnaBI TACGTA 1 cut(s) 547
Sse9I AATT 7 cut(s) 229, 260, 472, 675, 707, 734, 915
SsiI CCGC 6 cut(s) 346, 554, 620, 639, 663, 802
SspMI CTAG 1 cut(s) 566
StyD4I CCNGG 1 cut(s) 418
StyI CCWWGG 1 cut(s) 940
TaaI ACNGT 2 cut(s) 510, 743
TaiI ACGT 2 cut(s) 549, 891
TaqI TCGA 2 cut(s) 429, 906
TasI AATT 7 cut(s) 229, 260, 472, 675, 707, 734, 915
TatI WGTACW 1 cut(s) 952
TauI GCSGC 2 cut(s) 641, 665
TfiI GAWTC 4 cut(s) 201, 436, 531, 973
Tru1I TTAA 5 cut(s) 414, 674, 779, 885, 960
Tru9I TTAA 5 cut(s) 414, 674, 779, 885, 960
TscAI CASTG 2 cut(s) 329, 489
TseFI GTSAC 2 cut(s) 25, 502
TseI GCWGC 2 cut(s) 85, 796
Tsp45I GTSAC 2 cut(s) 25, 502
TspDTI ATGAA 4 cut(s) 232, 480, 698, 924
TspGWI ACGGA 1 cut(s) 95
TspRI CASTG 2 cut(s) 329, 489
VneI GTGCAC 1 cut(s) 561
VpaK11BI GGWCC 2 cut(s) 773, 857
VspI ATTAAT 1 cut(s) 779
XceI RCATGY 1 cut(s) 959
XcmI CCANNNNNNNNNTGG 1 cut(s) 619
XspI CTAG 1 cut(s) 566
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.