RchiOBHm_Chr4g0385981

transcriptional co-repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0030079
Physical Location & Seq
Forward (+)
1601669 .. 1604725
3057 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1041 bp
ATGACTAATGATTTTTTATCACTCAAGAATAACCGCAAAAGGAAAGGGCCTTCATCCTCTGGAGCTGCTAACAGTACTGGTACAGGAAATACAGTTGGTCCTTCATCTAATTCCCAACCGTCAACTCCATCCTTCAATGAAGTCGGTTCAATATGTAAAAGTAGTAGCAAAGTTGTCTGCTGTCACTTCTCTTCAGATGGGAAATTATTAGCCAGTGCAGGACATGACAAGAAGGTTGTTATTTGGAATATGGAAACACTACAGACTGAAAGCACACCCGAGGATCACAACCTGATAATTACTGATGTTCGATTCAGACCATATTCAACTCAGTTGGCAACTTCTTCATTTGACACAACTCCAAATTGTTTACAGACATATAAAGGACATACCTCGCATGTCATGTCACTTGATTTCCGCCCTAAGAAGACTGACCTTTTCTGCTCATGTGACCCGAACAATGAGATTCGCTTTTGGAATATTAATCAGTATTCGTGCATTCATGTTTCTAAGGGAGGTTCTTCACAGGTGAGGTTCCAGCCAAGAATTGGACAATTTATGGCTGTGGCATCTGGGAATGTTGTGTTAATATTCGATTCTGAGAGTGATGGGCAGACACATTCACTGCAGGGCCACTCCACAGGGGTGCACTCTTTTTGTTGGGATACAAATGGAGATTATTTGGCTTCAGTCAGTCAAGATTCTGTCAGAGTGTGGTCATTATCCTCTGGGAAGTGCATTCACGAGCTCAGTTCTAGTGGAAACATGTTCCATTCTTGTGTTTTCCATCCAAGCTACTCCAAGAGGTCATCGGAGGCTACCAGGCCTGGGCCTCCAGTTTTGGTCAGGTTACATGGTATGATGCTTGAAGATTTGCGGAATGGTATTGTGGATGAGTCAATGGAGCTCTGGAACATGGCTGTGAACAAGTGTATGACGGTTGCTGCTCATGACTCTGTAATATCAGCTTTGGCACAGTCACCAGTTACAAGGATGGTCGCTTCAGCCAGTCATGATAAATCCGTTAAAGTCTGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

346

Amino Acids

37.82

Weight (kDa)

8.27

Isoelectric Point (pI)

44.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 35 - 112 2.4e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 41 - 119 4.1e-10 THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 45 - 241 1.3e-06 MABP1/WDR62 second WD40 domain
WD40_Gbeta PF25391 46 - 181 1.7e-06 G protein beta WD-40 repeat protein
Beta-prop_CAF1B_HIR1 PF24105 47 - 199 4.4e-06 CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st PF24817 48 - 118 3.4e-08 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 50 - 89 3.2e-06 TEP-1 second beta-propeller
Beta-prop_EML_2 PF23414 52 - 165 2.5e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 54 - 169 9.1e-20 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 54 - 178 2.5e-08 WDR3 second beta-propeller domain
WD40 PF00400 55 - 83 2.3e-06 WD domain, G-beta repeat
WD40_Prp19 PF24814 56 - 260 2.4e-25 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 56 - 161 2.4e-14 WDR3 first beta-propeller domain
Beta-prop_WDR3_1st PF25173 122 - 264 4.9e-15 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 124 - 193 2.6e-08 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 125 - 264 5.1e-14 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 149 - 250 6.8e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 163 - 252 6.8e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 171 - 265 3.4e-14 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 185 - 264 1.3e-10 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 187 - 345 2.8e-07 CDC20/Fizzy WD40 domain
WD40 PF00400 206 - 240 1e-05 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 295 - 345 2.9e-06 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0030079)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0385981
rosa_samantha Rh4DG007600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 548
AciI CCGC 3 cut(s) 34, 418, 877
AclWI GGATC 1 cut(s) 291
AcuI CTGAAG 3 cut(s) 177, 672, 987
AfaI GTAC 2 cut(s) 76, 82
AfiI CCNNNNNNNGG 2 cut(s) 548, 828
AflIII ACRYGT 1 cut(s) 765
AgsI TTSAA 4 cut(s) 136, 150, 327, 869
AjnI CCWGG 2 cut(s) 821, 826
AleI CACNNNNGTG 1 cut(s) 644
AluBI AGCT 5 cut(s) 65, 748, 795, 907, 968
AluI AGCT 5 cut(s) 65, 748, 795, 907, 968
Alw21I GWGCWC 3 cut(s) 651, 750, 909
Alw44I GTGCAC 1 cut(s) 647
AlwI GGATC 1 cut(s) 291
Ama87I CYCGRG 1 cut(s) 278
AoxI GGCC 4 cut(s) 47, 631, 824, 830
ApaLI GTGCAC 1 cut(s) 647
ApeKI GCWGC 2 cut(s) 65, 944
AseI ATTAAT 1 cut(s) 483
AspS9I GGNCC 4 cut(s) 47, 98, 631, 830
AsuHPI GGTGA 2 cut(s) 541, 972
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 1 cut(s) 98
BaeGI GKGCMC 1 cut(s) 651
BanII GRGCYC 2 cut(s) 750, 909
BauI CACGAG 1 cut(s) 743
BbsI GAAGAC 1 cut(s) 434
Bbv12I GWGCWC 3 cut(s) 651, 750, 909
BbvI GCAGC 2 cut(s) 52, 931
BccI CCATC 5 cut(s) 136, 191, 602, 795, 988
BciT130I CCWGG 2 cut(s) 823, 828
BciVI GTATCC 1 cut(s) 658
BfaI CTAG 1 cut(s) 756
BfmI CTRYAG 2 cut(s) 260, 626
BfuI GTATCC 1 cut(s) 658
BisI GCNGC 2 cut(s) 66, 945
BlsI GCNGC 2 cut(s) 67, 946
BmcAI AGTACT 1 cut(s) 76
Bme1390I CCNGG 2 cut(s) 823, 828
Bme18I GGWCC 1 cut(s) 98
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 4 cut(s) 47, 98, 631, 830
BmiI GGNNCC 1 cut(s) 536
BmrFI CCNGG 2 cut(s) 823, 828
BmsI GCATC 2 cut(s) 578, 852
BpiI GAAGAC 1 cut(s) 434
BpmI CTGGAG 2 cut(s) 81, 819
BpuEI CTTGAG 1 cut(s) 8
BsaJI CCNNGG 2 cut(s) 279, 827
Bsc4I CCNNNNNNNGG 2 cut(s) 548, 828
Bse1I ACTGG 5 cut(s) 82, 213, 836, 983, 1008
BseBI CCWGG 2 cut(s) 823, 828
BseDI CCNNGG 2 cut(s) 279, 827
BseGI GGATG 5 cut(s) 53, 128, 787, 898, 999
BseLI CCNNNNNNNGG 2 cut(s) 548, 828
BseMII CTCAG 3 cut(s) 344, 591, 763
BseNI ACTGG 5 cut(s) 82, 213, 836, 983, 1008
BseSI GKGCMC 1 cut(s) 651
BseXI GCAGC 2 cut(s) 52, 931
BsgI GTGCAG 1 cut(s) 237
BshFI GGCC 4 cut(s) 49, 633, 826, 832
BsiHKAI GWGCWC 3 cut(s) 651, 750, 909
BsiHKCI CYCGRG 1 cut(s) 278
BslI CCNNNNNNNGG 2 cut(s) 548, 828
BsmI GAATGC 2 cut(s) 498, 738
BsnI GGCC 4 cut(s) 49, 633, 826, 832
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 3 cut(s) 651, 750, 909
Bsp143I GATC 1 cut(s) 283
BspACI CCGC 3 cut(s) 34, 418, 877
BspANI GGCC 4 cut(s) 49, 633, 826, 832
BspCNI CTCAG 3 cut(s) 343, 592, 762
BspHI TCATGA 2 cut(s) 949, 1012
BspLI GGNNCC 1 cut(s) 536
BspMAI CTGCAG 1 cut(s) 630
BspPI GGATC 1 cut(s) 291
BsrI ACTGG 5 cut(s) 82, 213, 836, 983, 1008
BssECI CCNNGG 2 cut(s) 279, 827
BssMI GATC 1 cut(s) 283
BssSI CACGAG 1 cut(s) 743
Bst2BI CACGAG 1 cut(s) 743
Bst2UI CCWGG 2 cut(s) 823, 828
Bst4CI ACNGT 5 cut(s) 74, 94, 120, 940, 978
Bst6I CTCTTC 1 cut(s) 196
BstDEI CTNAG 5 cut(s) 330, 423, 510, 600, 749
BstF5I GGATG 5 cut(s) 53, 128, 787, 898, 999
BstKTI GATC 1 cut(s) 286
BstMBI GATC 1 cut(s) 283
BstNI CCWGG 2 cut(s) 823, 828
BstNSI RCATGY 2 cut(s) 401, 769
BstSCI CCNGG 2 cut(s) 821, 826
BstSFI CTRYAG 2 cut(s) 260, 626
BstSLI GKGCMC 1 cut(s) 651
BstV1I GCAGC 2 cut(s) 52, 931
BstV2I GAAGAC 1 cut(s) 434
BsuI GTATCC 1 cut(s) 658
BsuRI GGCC 4 cut(s) 49, 633, 826, 832
BtsCI GGATG 5 cut(s) 53, 128, 787, 898, 999
BtsI GCAGTG 1 cut(s) 623
BtsIMutI CAGTG 2 cut(s) 220, 623
CciI TCATGA 2 cut(s) 949, 1012
Cfr13I GGNCC 4 cut(s) 47, 98, 631, 830
Csp6I GTAC 2 cut(s) 75, 81
CviQI GTAC 2 cut(s) 75, 81
DdeI CTNAG 5 cut(s) 330, 423, 510, 600, 749
DpnI GATC 1 cut(s) 285
DpnII GATC 1 cut(s) 283
Eam1104I CTCTTC 1 cut(s) 196
EarI CTCTTC 1 cut(s) 196
EciI GGCGGA 1 cut(s) 407
Ecl136II GAGCTC 2 cut(s) 748, 907
Eco147I AGGCCT 1 cut(s) 826
Eco24I GRGCYC 2 cut(s) 750, 909
Eco47I GGWCC 1 cut(s) 98
Eco53kI GAGCTC 2 cut(s) 748, 907
Eco57I CTGAAG 3 cut(s) 177, 672, 987
Eco88I CYCGRG 1 cut(s) 278
EcoICRI GAGCTC 2 cut(s) 748, 907
EcoO109I RGGNCCY 1 cut(s) 47
EcoRII CCWGG 2 cut(s) 821, 826
EcoT38I GRGCYC 2 cut(s) 750, 909
Fnu4HI GCNGC 2 cut(s) 66, 945
FokI GGATG 5 cut(s) 40, 115, 774, 905, 1006
FriOI GRGCYC 2 cut(s) 750, 909
Fsp4HI GCNGC 2 cut(s) 66, 945
FspBI CTAG 1 cut(s) 756
GluI GCNGC 2 cut(s) 66, 945
GsuI CTGGAG 2 cut(s) 81, 819
HaeIII GGCC 4 cut(s) 49, 633, 826, 832
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HinfI GANTC 6 cut(s) 312, 466, 596, 701, 896, 953
HphI GGTGA 2 cut(s) 541, 972
Hpy166II GTNNAC 4 cut(s) 123, 371, 649, 925
Hpy188I TCNGA 5 cut(s) 196, 317, 601, 710, 814
Hpy188III TCNNGA 8 cut(s) 25, 60, 698, 743, 910, 950, 1013, 1033
Hpy8I GTNNAC 4 cut(s) 123, 371, 649, 925
HpyAV CCTTC 4 cut(s) 60, 111, 142, 226
HpyCH4III ACNGT 5 cut(s) 74, 94, 120, 940, 978
HpyCH4V TGCA 5 cut(s) 218, 498, 628, 649, 738
HpyF3I CTNAG 5 cut(s) 330, 423, 510, 600, 749
Kzo9I GATC 1 cut(s) 283
LmnI GCTCC 2 cut(s) 62, 904
Lsp1109I GCAGC 2 cut(s) 52, 931
LweI GCATC 2 cut(s) 578, 852
MaeI CTAG 1 cut(s) 756
MaeIII GTNAC 6 cut(s) 182, 405, 449, 849, 978, 985
MalI GATC 1 cut(s) 285
MboI GATC 1 cut(s) 283
MboII GAAGA 5 cut(s) 183, 336, 439, 513, 881
MhlI GDGCHC 3 cut(s) 651, 750, 909
MluCI AATT 6 cut(s) 109, 203, 297, 364, 546, 554
MlyI GAGTC 2 cut(s) 905, 947
MnlI CCTC 9 cut(s) 67, 274, 403, 509, 525, 736, 798, 808, 843
MseI TTAA 3 cut(s) 483, 587, 1026
MslI CAYNNNNRTG 3 cut(s) 644, 777, 920
MspR9I CCNGG 2 cut(s) 823, 828
Mva1269I GAATGC 2 cut(s) 498, 738
MvaI CCWGG 2 cut(s) 823, 828
NdeII GATC 1 cut(s) 283
NlaIV GGNNCC 1 cut(s) 536
NmuCI GTSAC 4 cut(s) 182, 405, 449, 978
NspI RCATGY 2 cut(s) 401, 769
OliI CACNNNNGTG 1 cut(s) 644
PagI TCATGA 2 cut(s) 949, 1012
PceI AGGCCT 1 cut(s) 826
PciI ACATGT 1 cut(s) 765
PctI GAATGC 2 cut(s) 498, 738
PfeI GAWTC 4 cut(s) 312, 466, 596, 701
PflMI CCANNNNNTGG 1 cut(s) 548
PkrI GCNGC 2 cut(s) 67, 946
PleI GAGTC 2 cut(s) 904, 947
PpsI GAGTC 2 cut(s) 904, 947
PscI ACATGT 1 cut(s) 765
PshBI ATTAAT 1 cut(s) 483
Psp124BI GAGCTC 2 cut(s) 750, 909
Psp6I CCWGG 2 cut(s) 821, 826
PspGI CCWGG 2 cut(s) 821, 826
PspN4I GGNNCC 1 cut(s) 536
PspPI GGNCC 4 cut(s) 47, 98, 631, 830
PstI CTGCAG 1 cut(s) 630
RsaI GTAC 2 cut(s) 76, 82
RsaNI GTAC 2 cut(s) 75, 81
RseI CAYNNNNRTG 3 cut(s) 644, 777, 920
SacI GAGCTC 2 cut(s) 750, 909
SaqAI TTAA 3 cut(s) 483, 587, 1026
SatI GCNGC 2 cut(s) 66, 945
Sau3AI GATC 1 cut(s) 283
Sau96I GGNCC 4 cut(s) 47, 98, 631, 830
ScaI AGTACT 1 cut(s) 76
SchI GAGTC 2 cut(s) 905, 947
ScrFI CCNGG 2 cut(s) 823, 828
SduI GDGCHC 3 cut(s) 651, 750, 909
SfaNI GCATC 2 cut(s) 578, 852
SfcI CTRYAG 2 cut(s) 260, 626
SinI GGWCC 1 cut(s) 98
SmiMI CAYNNNNRTG 3 cut(s) 644, 777, 920
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 6 cut(s) 109, 203, 297, 364, 546, 554
SseBI AGGCCT 1 cut(s) 826
SsiI CCGC 3 cut(s) 34, 418, 877
SspI AATATT 2 cut(s) 481, 591
SspMI CTAG 1 cut(s) 756
SstI GAGCTC 2 cut(s) 750, 909
StuI AGGCCT 1 cut(s) 826
StyD4I CCNGG 2 cut(s) 821, 826
TaaI ACNGT 5 cut(s) 74, 94, 120, 940, 978
TaqI TCGA 2 cut(s) 310, 594
TasI AATT 6 cut(s) 109, 203, 297, 364, 546, 554
TatI WGTACW 1 cut(s) 74
TfiI GAWTC 4 cut(s) 312, 466, 596, 701
Tru1I TTAA 3 cut(s) 483, 587, 1026
Tru9I TTAA 3 cut(s) 483, 587, 1026
TscAI CASTG 2 cut(s) 220, 630
TseFI GTSAC 4 cut(s) 182, 405, 449, 978
TseI GCWGC 2 cut(s) 65, 944
Tsp45I GTSAC 4 cut(s) 182, 405, 449, 978
TspDTI ATGAA 5 cut(s) 42, 93, 153, 336, 491
TspGWI ACGGA 1 cut(s) 1012
TspRI CASTG 2 cut(s) 220, 630
Van91I CCANNNNNTGG 1 cut(s) 548
VneI GTGCAC 1 cut(s) 647
VpaK11BI GGWCC 1 cut(s) 98
VspI ATTAAT 1 cut(s) 483
XceI RCATGY 2 cut(s) 401, 769
XcmI CCANNNNNNNNNTGG 1 cut(s) 545
XspI CTAG 1 cut(s) 756
ZrmI AGTACT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.