RchiOBHm_Chr4g0387921

Perakine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
N/A
Physical Location & Seq
Reverse (-)
3090392 .. 3092446
2055 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
ATGGAGCACAAATCTGATATTGAAATCCCAAAGGTTAAACTGGGCAGTCAAGGCTTGGAGATTGGGTTTTGGATATGTGGGGGATTATCTGGGATATACAATGCTCCTCTCTCTCACGAAGCTGGATGTTCAGTGATAAAGGAAGCATTCAATAGGGGCATCACCTTCTATGATACTGCAGATGCTTATGGTCAGGATCACGATAGTGAAATCTTGGTCGGCAAGGCTTTGAAACAACTTCCTCGAGAAAATGTTCAATTGGCTACAAAATTTGGCATTTTCCAGTCAGAGAGCTTTGAATTCAGTGTAAATGGGACCCCGGAGTACCACCGTGTCAATGTTTCAGTGCCAATTGAGGATACGATTGGGGAGCTAGTGAATGAAGGAAAGATAAGATACATTGGCCTTTCAGAAGCTAGTATAGACACAATAACTAGAGCTCATGCTGTTCATCCCATTACTTCTGTGCAAATGGACTATTCTCTTTGGACTCGTGAAATCGAAAATGAGATAATTTCACTTTGCCGGCTTGTAAAATTTTCACCTCTGAATTCTACAATATCAAATTCCTCCTACTTTATTAATTCTAAGACTTGGATACGTAACCATGCATCCACATTTACAGGAAACTTGGAATTGGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

214

Amino Acids

23.79

Weight (kDa)

5.36

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 35 - 91 2.3e-10 Aldo/keto reductase family
Aldo_ket_red PF00248 110 - 177 2e-12 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 204
AcsI RAATTY 5 cut(s) 269, 299, 536, 550, 565
AfaI GTAC 1 cut(s) 326
AgsI TTSAA 5 cut(s) 23, 151, 232, 257, 299
AleI CACNNNNGTG 1 cut(s) 204
AluBI AGCT 5 cut(s) 122, 294, 373, 416, 440
AluI AGCT 5 cut(s) 122, 294, 373, 416, 440
Alw21I GWGCWC 2 cut(s) 9, 442
AlwI GGATC 1 cut(s) 204
Ama87I CYCGRG 1 cut(s) 243
AoxI GGCC 1 cut(s) 403
ApoI RAATTY 5 cut(s) 269, 299, 536, 550, 565
AseI ATTAAT 1 cut(s) 582
Asp700I GAANNNNTTC 1 cut(s) 252
AspS9I GGNCC 1 cut(s) 315
AsuC2I CCSGG 1 cut(s) 320
AsuHPI GGTGA 2 cut(s) 154, 534
AvaI CYCGRG 1 cut(s) 243
AvaII GGWCC 1 cut(s) 315
BanII GRGCYC 1 cut(s) 442
BauI CACGAG 1 cut(s) 492
Bbv12I GWGCWC 2 cut(s) 9, 442
BciVI GTATCC 2 cut(s) 352, 591
BcnI CCSGG 1 cut(s) 320
BfaI CTAG 3 cut(s) 374, 417, 435
BfmI CTRYAG 1 cut(s) 177
BfuI GTATCC 2 cut(s) 352, 591
Bme1390I CCNGG 1 cut(s) 320
Bme18I GGWCC 1 cut(s) 315
BmeT110I CYCGRG 1 cut(s) 243
BmgT120I GGNCC 1 cut(s) 315
BmiI GGNNCC 2 cut(s) 316, 317
BmrFI CCNGG 1 cut(s) 320
BmrI ACTGGG 1 cut(s) 50
BmsI GCATC 3 cut(s) 168, 172, 620
BmuI ACTGGG 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 320
BsaAI YACGTR 1 cut(s) 602
BsaJI CCNNGG 1 cut(s) 318
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bse118I RCCGGY 1 cut(s) 525
Bse1I ACTGG 2 cut(s) 45, 283
BseDI CCNNGG 1 cut(s) 318
BseGI GGATG 3 cut(s) 131, 451, 611
BseNI ACTGG 2 cut(s) 45, 283
BseRI GAGGAG 1 cut(s) 96
BshFI GGCC 1 cut(s) 405
BsiHKAI GWGCWC 2 cut(s) 9, 442
BsiHKCI CYCGRG 1 cut(s) 243
BsiSI CCGG 2 cut(s) 320, 526
BslFI GGGAC 1 cut(s) 328
BsmFI GGGAC 1 cut(s) 328
BsmI GAATGC 1 cut(s) 146
BsnI GGCC 1 cut(s) 405
BsoBI CYCGRG 1 cut(s) 243
Bsp1286I GDGCHC 2 cut(s) 9, 442
Bsp143I GATC 1 cut(s) 196
BspANI GGCC 1 cut(s) 405
BspLI GGNNCC 2 cut(s) 316, 317
BspMAI CTGCAG 1 cut(s) 181
BspPI GGATC 1 cut(s) 204
BsrFI RCCGGY 1 cut(s) 525
BsrI ACTGG 2 cut(s) 45, 283
BssAI RCCGGY 1 cut(s) 525
BssECI CCNNGG 1 cut(s) 318
BssMI GATC 1 cut(s) 196
BssSI CACGAG 1 cut(s) 492
Bst2BI CACGAG 1 cut(s) 492
Bst4CI ACNGT 1 cut(s) 332
BstBAI YACGTR 1 cut(s) 602
BstC8I GCNNGC 1 cut(s) 527
BstDEI CTNAG 1 cut(s) 588
BstF5I GGATG 3 cut(s) 131, 451, 611
BstKTI GATC 1 cut(s) 199
BstMBI GATC 1 cut(s) 196
BstMWI GCNNNNNNNGC 1 cut(s) 51
BstSCI CCNGG 1 cut(s) 318
BstSFI CTRYAG 1 cut(s) 177
BstSNI TACGTA 1 cut(s) 602
BsuI GTATCC 2 cut(s) 352, 591
BsuRI GGCC 1 cut(s) 405
BtsCI GGATG 3 cut(s) 131, 451, 611
BtsIMutI CAGTG 3 cut(s) 138, 310, 351
Cac8I GCNNGC 1 cut(s) 527
Cfr10I RCCGGY 1 cut(s) 525
Cfr13I GGNCC 1 cut(s) 315
Csp6I GTAC 1 cut(s) 325
CviAII CATG 2 cut(s) 443, 608
CviQI GTAC 1 cut(s) 325
DdeI CTNAG 1 cut(s) 588
DpnI GATC 1 cut(s) 198
DpnII GATC 1 cut(s) 196
Ecl136II GAGCTC 1 cut(s) 440
Eco105I TACGTA 1 cut(s) 602
Eco24I GRGCYC 1 cut(s) 442
Eco47I GGWCC 1 cut(s) 315
Eco53kI GAGCTC 1 cut(s) 440
Eco88I CYCGRG 1 cut(s) 243
EcoICRI GAGCTC 1 cut(s) 440
EcoO109I RGGNCCY 1 cut(s) 315
EcoRI GAATTC 2 cut(s) 299, 550
EcoT22I ATGCAT 1 cut(s) 613
EcoT38I GRGCYC 1 cut(s) 442
FaeI CATG 2 cut(s) 446, 611
FaiI YATR 7 cut(s) 76, 97, 171, 189, 422, 444, 609
FaqI GGGAC 1 cut(s) 328
FatI CATG 2 cut(s) 442, 607
FokI GGATG 3 cut(s) 138, 438, 598
FriOI GRGCYC 1 cut(s) 442
FspBI CTAG 3 cut(s) 374, 417, 435
HaeIII GGCC 1 cut(s) 405
HapII CCGG 2 cut(s) 320, 526
Hin1II CATG 2 cut(s) 446, 611
HinfI GANTC 1 cut(s) 490
HpaII CCGG 2 cut(s) 320, 526
HphI GGTGA 2 cut(s) 154, 534
Hpy188I TCNGA 4 cut(s) 16, 289, 412, 549
Hpy188III TCNNGA 5 cut(s) 116, 194, 200, 245, 494
HpyAV CCTTC 2 cut(s) 175, 377
HpyCH4III ACNGT 1 cut(s) 332
HpyCH4IV ACGT 1 cut(s) 601
HpyCH4V TGCA 3 cut(s) 179, 469, 611
HpyF10VI GCNNNNNNNGC 1 cut(s) 51
HpyF3I CTNAG 1 cut(s) 588
HpySE526I ACGT 1 cut(s) 601
Hsp92II CATG 2 cut(s) 446, 611
KflI GGGWCCC 1 cut(s) 315
KroI GCCGGC 1 cut(s) 525
KroNI GCCGGC 1 cut(s) 527
Kzo9I GATC 1 cut(s) 196
LmnI GCTCC 3 cut(s) 4, 109, 370
LpnPI CCDG 8 cut(s) 26, 75, 108, 179, 296, 333, 539, 609
LweI GCATC 3 cut(s) 168, 172, 620
MaeI CTAG 3 cut(s) 374, 417, 435
MaeII ACGT 1 cut(s) 601
MaeIII GTNAC 1 cut(s) 602
MalI GATC 1 cut(s) 198
MboI GATC 1 cut(s) 196
MfeI CAATTG 2 cut(s) 257, 351
MhlI GDGCHC 2 cut(s) 9, 442
MlyI GAGTC 1 cut(s) 484
MnlI CCTC 5 cut(s) 117, 252, 349, 555, 580
Mph1103I ATGCAT 1 cut(s) 613
MroNI GCCGGC 1 cut(s) 525
MroXI GAANNNNTTC 1 cut(s) 252
MseI TTAA 2 cut(s) 36, 582
MslI CAYNNNNRTG 1 cut(s) 204
MspI CCGG 2 cut(s) 320, 526
MspR9I CCNGG 1 cut(s) 320
MunI CAATTG 2 cut(s) 257, 351
Mva1269I GAATGC 1 cut(s) 146
MwoI GCNNNNNNNGC 1 cut(s) 51
NaeI GCCGGC 1 cut(s) 527
NciI CCSGG 1 cut(s) 320
NdeII GATC 1 cut(s) 196
NgoMIV GCCGGC 1 cut(s) 525
NlaIII CATG 2 cut(s) 446, 611
NlaIV GGNNCC 2 cut(s) 316, 317
NsiI ATGCAT 1 cut(s) 613
OliI CACNNNNGTG 1 cut(s) 204
PaeR7I CTCGAG 1 cut(s) 243
PctI GAATGC 1 cut(s) 146
PdiI GCCGGC 1 cut(s) 527
PdmI GAANNNNTTC 1 cut(s) 252
PleI GAGTC 1 cut(s) 484
PpsI GAGTC 1 cut(s) 484
Ppu21I YACGTR 1 cut(s) 602
PpuMI RGGWCCY 1 cut(s) 315
PshBI ATTAAT 1 cut(s) 582
Psp124BI GAGCTC 1 cut(s) 442
Psp5II RGGWCCY 1 cut(s) 315
PspN4I GGNNCC 2 cut(s) 316, 317
PspPI GGNCC 1 cut(s) 315
PspPPI RGGWCCY 1 cut(s) 315
PstI CTGCAG 1 cut(s) 181
RsaI GTAC 1 cut(s) 326
RsaNI GTAC 1 cut(s) 325
RseI CAYNNNNRTG 1 cut(s) 204
SacI GAGCTC 1 cut(s) 442
SaqAI TTAA 2 cut(s) 36, 582
Sau3AI GATC 1 cut(s) 196
Sau96I GGNCC 1 cut(s) 315
SchI GAGTC 1 cut(s) 484
ScrFI CCNGG 1 cut(s) 320
SduI GDGCHC 2 cut(s) 9, 442
SetI ASST 9 cut(s) 36, 124, 167, 296, 375, 418, 442, 547, 604
SfaNI GCATC 3 cut(s) 168, 172, 620
SfcI CTRYAG 1 cut(s) 177
Sfr274I CTCGAG 1 cut(s) 243
SinI GGWCC 1 cut(s) 315
SlaI CTCGAG 1 cut(s) 243
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 243
SmoI CTYRAG 1 cut(s) 243
SnaBI TACGTA 1 cut(s) 602
SspMI CTAG 3 cut(s) 374, 417, 435
SstI GAGCTC 1 cut(s) 442
StyD4I CCNGG 1 cut(s) 318
TaaI ACNGT 1 cut(s) 332
TaiI ACGT 1 cut(s) 604
TaqI TCGA 2 cut(s) 244, 501
Tru1I TTAA 2 cut(s) 36, 582
Tru9I TTAA 2 cut(s) 36, 582
TscAI CASTG 3 cut(s) 138, 310, 351
TspDTI ATGAA 2 cut(s) 396, 440
TspRI CASTG 3 cut(s) 138, 310, 351
VpaK11BI GGWCC 1 cut(s) 315
VspI ATTAAT 1 cut(s) 582
XapI RAATTY 5 cut(s) 269, 299, 536, 550, 565
XhoI CTCGAG 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 252
XspI CTAG 3 cut(s) 374, 417, 435
Zsp2I ATGCAT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.