RchiOBHm_Chr4g0392181

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0030076
Physical Location & Seq
Reverse (-)
7575875 .. 7576598
724 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 357 bp
ATGGCTCCAGAGCCTCTTGATCCTCAAGAACTGCCTATGGTTTGCAGAGATATAACTATGGAGTACTCTCAACATGTTTTCAAACTGAGTGTGACTTTGTTTGAATTGCTATCCGAGGCGCTTAAGCTCAAACTTGACCACCTTATAAACTTGGATTGTGCGAAGGGGCATTCATTGCTTAGCCACTACTATCCACCATGCCCTGAGCCTGAACTCACCATGGGCACCAGCAAACATTTTGAGGCTGCTTTTCGCACCATCCTACTTCAGGACCACATCGGTAAACCCCAGCGACCAAGCACTCCTGCCCCAAAAGCGGAGACCGAACAGCCGCCAGGTTGTCGAGGACACGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

118

Amino Acids

13.26

Weight (kDa)

5.93

Isoelectric Point (pI)

50.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 57 - 95 9.6e-08 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030076)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0392181
rosa_samantha Rh4BG035600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 146
AccB1I GGYRCC 1 cut(s) 224
AciI CCGC 2 cut(s) 317, 332
AclWI GGATC 1 cut(s) 14
AcuI CTGAAG 1 cut(s) 251
AfaI GTAC 1 cut(s) 65
AfiI CCNNNNNNNGG 2 cut(s) 268, 316
AflII CTTAAG 1 cut(s) 122
AflIII ACRYGT 2 cut(s) 73, 349
AgsI TTSAA 2 cut(s) 82, 104
AjiI CACGTC 1 cut(s) 352
AjnI CCWGG 1 cut(s) 334
AluBI AGCT 1 cut(s) 127
AluI AGCT 1 cut(s) 127
Alw26I GTCTC 1 cut(s) 314
AlwI GGATC 1 cut(s) 14
ApeKI GCWGC 1 cut(s) 245
AspLEI GCGC 1 cut(s) 121
AspS9I GGNCC 1 cut(s) 271
AsuHPI GGTGA 1 cut(s) 208
AvaII GGWCC 1 cut(s) 271
BaeGI GKGCMC 1 cut(s) 227
BanI GGYRCC 1 cut(s) 224
BbvI GCAGC 1 cut(s) 232
BccI CCATC 1 cut(s) 266
BciT130I CCWGG 1 cut(s) 336
BcoDI GTCTC 1 cut(s) 314
BfoI RGCGCY 1 cut(s) 122
BfrI CTTAAG 1 cut(s) 122
BisI GCNGC 2 cut(s) 246, 332
BlpI GCTNAGC 1 cut(s) 179
BlsI GCNGC 2 cut(s) 247, 333
BmcAI AGTACT 1 cut(s) 65
Bme1390I CCNGG 1 cut(s) 336
Bme18I GGWCC 1 cut(s) 271
BmgBI CACGTC 1 cut(s) 352
BmgT120I GGNCC 1 cut(s) 271
BmiI GGNNCC 2 cut(s) 6, 226
BmrFI CCNGG 1 cut(s) 336
Bpu10I CCTNAGC 1 cut(s) 204
Bpu1102I GCTNAGC 1 cut(s) 179
BpuEI CTTGAG 1 cut(s) 9
BsaI GGTCTC 1 cut(s) 314
BsaJI CCNNGG 2 cut(s) 114, 219
Bsc4I CCNNNNNNNGG 2 cut(s) 268, 316
Bse3DI GCAATG 1 cut(s) 173
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 114, 219
BseGI GGATG 1 cut(s) 258
BseLI CCNNNNNNNGG 2 cut(s) 268, 316
BseMI GCAATG 1 cut(s) 173
BseMII CTCAG 2 cut(s) 77, 195
BseSI GKGCMC 1 cut(s) 227
BseXI GCAGC 1 cut(s) 232
BseYI CCCAGC 1 cut(s) 288
BshNI GGYRCC 1 cut(s) 224
BslI CCNNNNNNNGG 2 cut(s) 268, 316
BsmAI GTCTC 1 cut(s) 314
BsmI GAATGC 1 cut(s) 169
Bso31I GGTCTC 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 227
Bsp143I GATC 1 cut(s) 19
Bsp1720I GCTNAGC 1 cut(s) 179
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 2 cut(s) 317, 332
BspCNI CTCAG 2 cut(s) 78, 196
BspLI GGNNCC 2 cut(s) 6, 226
BspPI GGATC 1 cut(s) 14
BspT107I GGYRCC 1 cut(s) 224
BspTI CTTAAG 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 314
BsrDI GCAATG 1 cut(s) 173
BssECI CCNNGG 2 cut(s) 114, 219
BssMI GATC 1 cut(s) 19
BssT1I CCWWGG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 336
BstAFI CTTAAG 1 cut(s) 122
BstAPI GCANNNNNTGC 1 cut(s) 175
BstDEI CTNAG 3 cut(s) 86, 179, 204
BstDSI CCRYGG 1 cut(s) 219
BstENI CCTNNNNNAGG 1 cut(s) 266
BstF5I GGATG 1 cut(s) 258
BstH2I RGCGCY 1 cut(s) 122
BstHHI GCGC 1 cut(s) 121
BstKTI GATC 1 cut(s) 22
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 1 cut(s) 19
BstMWI GCNNNNNNNGC 2 cut(s) 175, 314
BstNI CCWGG 1 cut(s) 336
BstNSI RCATGY 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 334
BstSLI GKGCMC 1 cut(s) 227
BstV1I GCAGC 1 cut(s) 232
BtgI CCRYGG 1 cut(s) 219
BtrI CACGTC 1 cut(s) 352
BtsCI GGATG 1 cut(s) 258
CfoI GCGC 1 cut(s) 121
Cfr13I GGNCC 1 cut(s) 271
Csp6I GTAC 1 cut(s) 64
CviAII CATG 3 cut(s) 74, 198, 220
CviJI RGCY 7 cut(s) 5, 13, 127, 183, 208, 245, 331
CviKI_1 RGCY 7 cut(s) 5, 13, 127, 183, 208, 245, 331
CviQI GTAC 1 cut(s) 64
DdeI CTNAG 3 cut(s) 86, 179, 204
DpnI GATC 1 cut(s) 21
DpnII GATC 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 219
Eco31I GGTCTC 1 cut(s) 314
Eco47I GGWCC 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 251
EcoNI CCTNNNNNAGG 1 cut(s) 266
EcoRII CCWGG 1 cut(s) 334
EcoT14I CCWWGG 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 3 cut(s) 77, 201, 223
FaiI YATR 7 cut(s) 38, 53, 59, 75, 146, 199, 221
FatI CATG 3 cut(s) 73, 197, 219
Fnu4HI GCNGC 2 cut(s) 246, 332
FokI GGATG 1 cut(s) 245
Fsp4HI GCNGC 2 cut(s) 246, 332
GlaI GCGC 1 cut(s) 120
GluI GCNGC 2 cut(s) 246, 332
GsaI CCCAGC 1 cut(s) 292
HaeII RGCGCY 1 cut(s) 122
HhaI GCGC 1 cut(s) 121
Hin1II CATG 3 cut(s) 77, 201, 223
Hin6I GCGC 1 cut(s) 119
HinP1I GCGC 1 cut(s) 119
HphI GGTGA 1 cut(s) 208
Hpy166II GTNNAC 1 cut(s) 284
Hpy188I TCNGA 2 cut(s) 115, 356
Hpy188III TCNNGA 4 cut(s) 8, 17, 26, 269
Hpy8I GTNNAC 1 cut(s) 284
HpyAV CCTTC 1 cut(s) 157
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 1 cut(s) 45
HpyF10VI GCNNNNNNNGC 2 cut(s) 175, 314
HpyF3I CTNAG 3 cut(s) 86, 179, 204
HpySE526I ACGT 1 cut(s) 351
Hsp92II CATG 3 cut(s) 77, 201, 223
HspAI GCGC 1 cut(s) 119
Kzo9I GATC 1 cut(s) 19
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 9 cut(s) 21, 216, 222, 241, 254, 302, 318, 321, 348
Lsp1109I GCAGC 1 cut(s) 232
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 1 cut(s) 91
MalI GATC 1 cut(s) 21
MboI GATC 1 cut(s) 19
MhlI GDGCHC 1 cut(s) 227
MluCI AATT 1 cut(s) 104
MnlI CCTC 5 cut(s) 24, 33, 109, 235, 338
MseI TTAA 1 cut(s) 123
MspCI CTTAAG 1 cut(s) 122
MspR9I CCNGG 1 cut(s) 336
Mva1269I GAATGC 1 cut(s) 169
MvaI CCWGG 1 cut(s) 336
MwoI GCNNNNNNNGC 2 cut(s) 175, 314
NcoI CCATGG 1 cut(s) 219
NdeII GATC 1 cut(s) 19
NlaIII CATG 3 cut(s) 77, 201, 223
NlaIV GGNNCC 2 cut(s) 6, 226
NmuCI GTSAC 1 cut(s) 91
NspI RCATGY 1 cut(s) 77
PciI ACATGT 1 cut(s) 73
PctI GAATGC 1 cut(s) 169
PkrI GCNGC 2 cut(s) 247, 333
PscI ACATGT 1 cut(s) 73
PsiI TTATAA 1 cut(s) 146
Psp6I CCWGG 1 cut(s) 334
PspFI CCCAGC 1 cut(s) 288
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 2 cut(s) 6, 226
PspPI GGNCC 1 cut(s) 271
RsaI GTAC 1 cut(s) 65
RsaNI GTAC 1 cut(s) 64
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 2 cut(s) 246, 332
Sau3AI GATC 1 cut(s) 19
Sau96I GGNCC 1 cut(s) 271
ScaI AGTACT 1 cut(s) 65
ScrFI CCNGG 1 cut(s) 336
SduI GDGCHC 1 cut(s) 227
SetI ASST 4 cut(s) 129, 144, 340, 354
SinI GGWCC 1 cut(s) 271
SmlI CTYRAG 2 cut(s) 24, 122
SmoI CTYRAG 2 cut(s) 24, 122
Sse9I AATT 1 cut(s) 104
SsiI CCGC 2 cut(s) 317, 332
StyD4I CCNGG 1 cut(s) 334
StyI CCWWGG 1 cut(s) 219
TaiI ACGT 1 cut(s) 354
TaqI TCGA 1 cut(s) 343
TaqII GACCGA 1 cut(s) 338
TasI AATT 1 cut(s) 104
TatI WGTACW 1 cut(s) 63
TauI GCSGC 1 cut(s) 334
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 1 cut(s) 245
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 1 cut(s) 162
Vha464I CTTAAG 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 271
XagI CCTNNNNNAGG 1 cut(s) 266
XceI RCATGY 1 cut(s) 77
ZrmI AGTACT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.