RchiOBHm_Chr4g0399231

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0030048
Physical Location & Seq
Forward (+)
16400062 .. 16401083
1022 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 363 bp
ATGCTTACTATGGAGCCAAAATTTGGAAAGATGCTCTTGGTAGGTGCCATCTTCAACTGCCTCGATCCTGTTTTGACTATTGTTTCCGGTCTTAGTGTCAGAGATCCTTTCTTAACACCATTTGACAAGGAAGATCTTGCGGATGCTGCTAAATCTCAGTTTTCCCGAGACCACAGTGATCACCTTGCACTTGTGCGTGCCTATGAGGGCTGGAAGGTAGCTGAAAGAGACTTTGCTGGATATGACTACTGCTGGAAAAATTTTCTTTCAGCACAATCAATGAAAGCTATTGATTCTCTCAGGAAGGAGTTCTACTCTTTGAAAGCTAGTGCCTACACCTCACACACACACATATATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

120

Amino Acids

13.66

Weight (kDa)

6.4

Isoelectric Point (pI)

33.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HA2_C PF21010 5 - 64 1.4e-17 Helicase associated domain (HA2), ratchet-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0030048)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0399231
rosa_multiflora Rmu_sc0002011.1_g000021

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 44
AccB7I CCANNNNNTGG 1 cut(s) 23
AciI CCGC 1 cut(s) 140
AclWI GGATC 2 cut(s) 59, 98
AcsI RAATTY 2 cut(s) 20, 259
AfiI CCNNNNNNNGG 1 cut(s) 23
AgsI TTSAA 2 cut(s) 55, 322
AluBI AGCT 3 cut(s) 221, 287, 326
AluI AGCT 3 cut(s) 221, 287, 326
Alw26I GTCTC 2 cut(s) 162, 222
AlwI GGATC 2 cut(s) 59, 98
Ama87I CYCGRG 1 cut(s) 165
ApeKI GCWGC 1 cut(s) 146
ApoI RAATTY 2 cut(s) 20, 259
Asp700I GAANNNNTTC 1 cut(s) 308
AsuHPI GGTGA 1 cut(s) 173
AvaI CYCGRG 1 cut(s) 165
BanI GGYRCC 1 cut(s) 44
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
BbvI GCAGC 1 cut(s) 133
BccI CCATC 1 cut(s) 56
BclI TGATCA 1 cut(s) 178
BcoDI GTCTC 2 cut(s) 162, 222
BfaI CTAG 1 cut(s) 327
BglII AGATCT 1 cut(s) 133
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
BmeT110I CYCGRG 1 cut(s) 165
BmiI GGNNCC 2 cut(s) 15, 46
BmsI GCATC 2 cut(s) 21, 133
BplI GAGNNNNNCTC 2 cut(s) 299, 331
BsaI GGTCTC 1 cut(s) 162
BsaWI WCCGGW 1 cut(s) 86
Bsc4I CCNNNNNNNGG 1 cut(s) 23
BseGI GGATG 1 cut(s) 148
BseLI CCNNNNNNNGG 1 cut(s) 23
BseMII CTCAG 2 cut(s) 170, 313
BseXI GCAGC 1 cut(s) 133
BshNI GGYRCC 1 cut(s) 44
BsiHKCI CYCGRG 1 cut(s) 165
BsiSI CCGG 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 23
BsmAI GTCTC 2 cut(s) 162, 222
Bso31I GGTCTC 1 cut(s) 162
BsoBI CYCGRG 1 cut(s) 165
Bsp143I GATC 4 cut(s) 64, 103, 133, 178
BspACI CCGC 1 cut(s) 140
BspCNI CTCAG 2 cut(s) 169, 312
BspLI GGNNCC 2 cut(s) 15, 46
BspPI GGATC 2 cut(s) 59, 98
BspT107I GGYRCC 1 cut(s) 44
BspTNI GGTCTC 1 cut(s) 162
BssMI GATC 4 cut(s) 64, 103, 133, 178
Bst4CI ACNGT 1 cut(s) 176
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 3 cut(s) 92, 156, 299
BstF5I GGATG 1 cut(s) 148
BstKTI GATC 4 cut(s) 67, 106, 136, 181
BstMAI GTCTC 2 cut(s) 162, 222
BstMBI GATC 4 cut(s) 64, 103, 133, 178
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstV1I GCAGC 1 cut(s) 133
BstX2I RGATCY 2 cut(s) 103, 133
BstYI RGATCY 2 cut(s) 103, 133
BtsCI GGATG 1 cut(s) 148
BtsIMutI CAGTG 1 cut(s) 181
Cac8I GCNNGC 1 cut(s) 198
CviJI RGCY 5 cut(s) 16, 210, 221, 287, 326
CviKI_1 RGCY 5 cut(s) 16, 210, 221, 287, 326
DdeI CTNAG 3 cut(s) 92, 156, 299
DpnI GATC 4 cut(s) 66, 105, 135, 180
DpnII GATC 4 cut(s) 64, 103, 133, 178
Eco31I GGTCTC 1 cut(s) 162
Eco88I CYCGRG 1 cut(s) 165
FaiI YATR 8 cut(s) 11, 204, 243, 353, 355, 357, 359, 361
FalI AAGNNNNNCTT 2 cut(s) 20, 52
FbaI TGATCA 1 cut(s) 178
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 1 cut(s) 155
Fsp4HI GCNGC 1 cut(s) 147
FspBI CTAG 1 cut(s) 327
GluI GCNGC 1 cut(s) 147
HapII CCGG 1 cut(s) 87
HinfI GANTC 1 cut(s) 293
HpaII CCGG 1 cut(s) 87
HphI GGTGA 1 cut(s) 173
Hpy188I TCNGA 1 cut(s) 101
Hpy188III TCNNGA 2 cut(s) 165, 301
HpyAV CCTTC 2 cut(s) 208, 298
HpyCH4III ACNGT 1 cut(s) 176
HpyCH4V TGCA 1 cut(s) 188
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 3 cut(s) 92, 156, 299
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 4 cut(s) 64, 103, 133, 178
LmnI GCTCC 1 cut(s) 13
LpnPI CCDG 6 cut(s) 81, 100, 196, 222, 238, 286
Lsp1109I GCAGC 1 cut(s) 133
LweI GCATC 2 cut(s) 21, 133
MaeI CTAG 1 cut(s) 327
MalI GATC 4 cut(s) 66, 105, 135, 180
MboI GATC 4 cut(s) 64, 103, 133, 178
MboII GAAGA 2 cut(s) 43, 143
MflI RGATCY 2 cut(s) 103, 133
MluCI AATT 2 cut(s) 20, 259
MnlI CCTC 3 cut(s) 71, 199, 349
MroXI GAANNNNTTC 1 cut(s) 308
MseI TTAA 1 cut(s) 113
MspI CCGG 1 cut(s) 87
MwoI GCNNNNNNNGC 1 cut(s) 146
NdeII GATC 4 cut(s) 64, 103, 133, 178
NlaIV GGNNCC 2 cut(s) 15, 46
PdmI GAANNNNTTC 1 cut(s) 308
PfeI GAWTC 1 cut(s) 293
PflMI CCANNNNNTGG 1 cut(s) 23
PkrI GCNGC 1 cut(s) 148
PspN4I GGNNCC 2 cut(s) 15, 46
PsuI RGATCY 2 cut(s) 103, 133
SaqAI TTAA 1 cut(s) 113
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 4 cut(s) 64, 103, 133, 178
SetI ASST 7 cut(s) 46, 186, 219, 223, 289, 328, 341
SfaNI GCATC 2 cut(s) 21, 133
Sse9I AATT 2 cut(s) 20, 259
SsiI CCGC 1 cut(s) 140
SspMI CTAG 1 cut(s) 327
TaaI ACNGT 1 cut(s) 176
TaqI TCGA 1 cut(s) 63
TasI AATT 2 cut(s) 20, 259
TfiI GAWTC 1 cut(s) 293
Tru1I TTAA 1 cut(s) 113
Tru9I TTAA 1 cut(s) 113
TscAI CASTG 1 cut(s) 181
TseI GCWGC 1 cut(s) 146
TspDTI ATGAA 1 cut(s) 296
TspRI CASTG 1 cut(s) 181
Van91I CCANNNNNTGG 1 cut(s) 23
XapI RAATTY 2 cut(s) 20, 259
XmnI GAANNNNTTC 1 cut(s) 308
XspI CTAG 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.