RchiOBHm_Chr4g0400951

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
N/A
Physical Location & Seq
Forward (+)
18913583 .. 18915612
2030 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 951 bp
ATGAATGAGAGAAAGAAGCACGTCTACACCGCTAAGCTCGCCGAGCAAGCCGAGCGCTACGATGAAATGCTGGAGGCGATGACGAAAGTGGTGAAGCTTGGCGTGGAGCTCACTGTCGAGGAGCGAAACCTTCTGTCTGTGGGGTACAAGAACGTGATCGGAGCTCGTAGGGCTTCGTGGAGGATACTGTCGTCAATCAAGCAGAAGGAGGAGAGCAAAGGGAATGAGCAGAATGTGAGCCGTATCAAGGAGTTCAGCAACAAGGTGGAGACTGAGCTCCCGAGCATTTGCACTGACATCATGACGGTTATTGATGAGCATCTCCTTCCATCTTGCTCAGATTTCGAGTCCACTGTGTTCTTCTACCAAATGAAAGGAGATTACTATCGCTACTTGGCAGAGTTTAAGACTGGCGATGAGAGGAAAAAGGTTGCTGATCAGTCAATGGAAGCTTATCAGGCAGCTTCTAGCGAAGCAGAGACTCATTTACCTCCCACACATCCCATCAGGCTGAGATTGGCCTTGAACTTCTCTGTCTTTTACTATGAAATCTTGAACTCTCCTGAAAGAGCTTGTCACCTTGCAAAGCAAGCCTTTGATGAAGCTATCTCTGAGCTGGATACTCTCTATGAGGAATCATACAAGGGCAGCACCTTAATCATGGAACTCTTGAGGGACAACCTTACATTGTGGACTTCTGACATTCCAGAGAATGGAGGTAATTATAAGTTAAGCACGCTACCTTGTGCATCTGTATCGGAAGAGTTTGTCTCAAGTCCGGTTTTTATCTTCTTCTTCTTTTTGTCCTTTTATCTCGATGATGGTGGCTTTCTGGATGATGTCAGTTCATGTGGATGGATTAGTAAGGACTTGTTGTATTACTCTGCTGCAGTTGAAGAAGTGAAGAAGCTGGACCAGTATCTAGGTGGTGCCTTCTTTTGCAAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

316

Amino Acids

36.12

Weight (kDa)

4.92

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 10 - 232 3e-102 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 726
AccB1I GGYRCC 1 cut(s) 929
AccB7I CCANNNNNTGG 1 cut(s) 713
AccI GTMKAC 1 cut(s) 24
AciI CCGC 1 cut(s) 30
AfaI GTAC 1 cut(s) 146
AfeI AGCGCT 1 cut(s) 56
AfiI CCNNNNNNNGG 2 cut(s) 247, 713
AgsI TTSAA 3 cut(s) 526, 556, 896
AjiI CACGTC 1 cut(s) 22
Alw21I GWGCWC 3 cut(s) 111, 166, 279
Alw26I GTCTC 3 cut(s) 263, 473, 775
Ama87I CYCGRG 1 cut(s) 280
Aor51HI AGCGCT 1 cut(s) 56
AoxI GGCC 1 cut(s) 519
ApeKI GCWGC 3 cut(s) 461, 648, 887
AspLEI GCGC 1 cut(s) 57
AspS9I GGNCC 1 cut(s) 913
AsuHPI GGTGA 2 cut(s) 103, 569
AvaI CYCGRG 1 cut(s) 280
AvaII GGWCC 1 cut(s) 913
BaeI ACNNNNGTAYC 2 cut(s) 176, 209
BanI GGYRCC 1 cut(s) 929
BanII GRGCYC 3 cut(s) 111, 166, 279
BarI GAAGNNNNNNTAC 2 cut(s) 8, 40
Bbv12I GWGCWC 3 cut(s) 111, 166, 279
BbvI GCAGC 3 cut(s) 473, 660, 874
BccI CCATC 4 cut(s) 337, 512, 815, 849
BceAI ACGGC 1 cut(s) 225
BcgI CGANNNNNNTGC 2 cut(s) 738, 772
BciVI GTATCC 2 cut(s) 177, 613
BclI TGATCA 1 cut(s) 436
BcoDI GTCTC 3 cut(s) 263, 473, 775
BfaI CTAG 2 cut(s) 468, 923
BfmI CTRYAG 1 cut(s) 888
BfoI RGCGCY 1 cut(s) 58
BfuI GTATCC 2 cut(s) 177, 613
BisI GCNGC 3 cut(s) 462, 649, 888
BlpI GCTNAGC 1 cut(s) 33
BlsI GCNGC 3 cut(s) 463, 650, 889
Bme18I GGWCC 1 cut(s) 913
BmeT110I CYCGRG 1 cut(s) 280
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 913
BmiI GGNNCC 1 cut(s) 931
BmsI GCATC 2 cut(s) 328, 758
BplI GAGNNNNNCTC 2 cut(s) 755, 787
BpmI CTGGAG 1 cut(s) 92
Bpu1102I GCTNAGC 1 cut(s) 33
BpuEI CTTGAG 2 cut(s) 691, 757
BsaBI GATNNNNATC 2 cut(s) 318, 384
BsaWI WCCGGW 1 cut(s) 778
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 713
Bse1I ACTGG 2 cut(s) 415, 916
Bse8I GATNNNNATC 2 cut(s) 318, 384
BseGI GGATG 3 cut(s) 499, 841, 860
BseJI GATNNNNATC 2 cut(s) 318, 384
BseLI CCNNNNNNNGG 2 cut(s) 247, 713
BseMII CTCAG 4 cut(s) 264, 351, 503, 603
BseNI ACTGG 2 cut(s) 415, 916
BseRI GAGGAG 2 cut(s) 134, 224
BseXI GCAGC 3 cut(s) 473, 660, 874
BshFI GGCC 1 cut(s) 521
BshNI GGYRCC 1 cut(s) 929
BsiHKAI GWGCWC 3 cut(s) 111, 166, 279
BsiHKCI CYCGRG 1 cut(s) 280
BsiSI CCGG 1 cut(s) 779
BslFI GGGAC 1 cut(s) 689
BslI CCNNNNNNNGG 2 cut(s) 247, 713
BsmAI GTCTC 3 cut(s) 263, 473, 775
BsmFI GGGAC 1 cut(s) 689
BsnI GGCC 1 cut(s) 521
BsoBI CYCGRG 1 cut(s) 280
Bsp1286I GDGCHC 3 cut(s) 111, 166, 279
Bsp143I GATC 2 cut(s) 156, 436
Bsp1720I GCTNAGC 1 cut(s) 33
BspACI CCGC 1 cut(s) 30
BspANI GGCC 1 cut(s) 521
BspCNI CTCAG 4 cut(s) 265, 350, 504, 604
BspHI TCATGA 1 cut(s) 300
BspLI GGNNCC 1 cut(s) 931
BspMAI CTGCAG 1 cut(s) 892
BspT107I GGYRCC 1 cut(s) 929
BsrI ACTGG 2 cut(s) 415, 916
BssMI GATC 2 cut(s) 156, 436
Bst4CI ACNGT 4 cut(s) 115, 189, 307, 355
Bst6I CTCTTC 1 cut(s) 756
BstC8I GCNNGC 4 cut(s) 39, 48, 591, 737
BstDEI CTNAG 5 cut(s) 33, 273, 337, 512, 612
BstF5I GGATG 3 cut(s) 499, 841, 860
BstH2I RGCGCY 1 cut(s) 58
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 2 cut(s) 159, 439
BstMAI GTCTC 3 cut(s) 263, 473, 775
BstMBI GATC 2 cut(s) 156, 436
BstMWI GCNNNNNNNGC 7 cut(s) 38, 43, 47, 52, 170, 458, 590
BstSFI CTRYAG 1 cut(s) 888
BstV1I GCAGC 3 cut(s) 473, 660, 874
BsuI GTATCC 2 cut(s) 177, 613
BsuRI GGCC 1 cut(s) 521
BtgZI GCGATG 2 cut(s) 92, 429
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 3 cut(s) 499, 841, 860
BtsIMutI CAGTG 3 cut(s) 111, 291, 351
Cac8I GCNNGC 4 cut(s) 39, 48, 591, 737
CciI TCATGA 1 cut(s) 300
CfoI GCGC 1 cut(s) 57
Cfr13I GGNCC 1 cut(s) 913
Csp6I GTAC 1 cut(s) 145
CviAII CATG 3 cut(s) 301, 661, 849
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 5 cut(s) 33, 273, 337, 512, 612
DpnI GATC 2 cut(s) 158, 438
DpnII GATC 2 cut(s) 156, 436
Eam1104I CTCTTC 1 cut(s) 756
EarI CTCTTC 1 cut(s) 756
Ecl136II GAGCTC 3 cut(s) 109, 164, 277
Eco24I GRGCYC 3 cut(s) 111, 166, 279
Eco47I GGWCC 1 cut(s) 913
Eco47III AGCGCT 1 cut(s) 56
Eco53kI GAGCTC 3 cut(s) 109, 164, 277
Eco88I CYCGRG 1 cut(s) 280
EcoICRI GAGCTC 3 cut(s) 109, 164, 277
EcoT38I GRGCYC 3 cut(s) 111, 166, 279
FaeI CATG 3 cut(s) 304, 664, 852
FaiI YATR 7 cut(s) 302, 546, 630, 640, 662, 726, 850
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FaqI GGGAC 1 cut(s) 689
FatI CATG 3 cut(s) 300, 660, 848
FbaI TGATCA 1 cut(s) 436
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 3 cut(s) 462, 649, 888
FokI GGATG 3 cut(s) 486, 848, 867
FriOI GRGCYC 3 cut(s) 111, 166, 279
Fsp4HI GCNGC 3 cut(s) 462, 649, 888
FspBI CTAG 2 cut(s) 468, 923
GlaI GCGC 1 cut(s) 56
GluI GCNGC 3 cut(s) 462, 649, 888
GsuI CTGGAG 1 cut(s) 92
HaeII RGCGCY 1 cut(s) 58
HaeIII GGCC 1 cut(s) 521
HapII CCGG 1 cut(s) 779
HhaI GCGC 1 cut(s) 57
Hin1II CATG 3 cut(s) 304, 664, 852
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HindIII AAGCTT 2 cut(s) 95, 450
HinfI GANTC 3 cut(s) 347, 481, 635
HpaII CCGG 1 cut(s) 779
HphI GGTGA 2 cut(s) 103, 569
Hpy166II GTNNAC 3 cut(s) 25, 351, 693
Hpy188I TCNGA 5 cut(s) 161, 340, 613, 700, 760
Hpy188III TCNNGA 8 cut(s) 280, 301, 553, 563, 670, 707, 815, 833
Hpy8I GTNNAC 3 cut(s) 25, 351, 693
HpyAV CCTTC 4 cut(s) 140, 199, 335, 943
HpyCH4III ACNGT 4 cut(s) 115, 189, 307, 355
HpyCH4IV ACGT 2 cut(s) 21, 153
HpyCH4V TGCA 5 cut(s) 291, 584, 749, 890, 942
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 43, 47, 52, 170, 458, 590
HpyF3I CTNAG 5 cut(s) 33, 273, 337, 512, 612
HpySE526I ACGT 2 cut(s) 21, 153
Hsp92II CATG 3 cut(s) 304, 664, 852
HspAI GCGC 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 436
Kzo9I GATC 2 cut(s) 156, 436
LmnI GCTCC 4 cut(s) 106, 121, 161, 282
Lsp1109I GCAGC 3 cut(s) 473, 660, 874
LweI GCATC 2 cut(s) 328, 758
MaeI CTAG 2 cut(s) 468, 923
MaeII ACGT 2 cut(s) 21, 153
MaeIII GTNAC 1 cut(s) 575
MalI GATC 2 cut(s) 158, 438
MboI GATC 2 cut(s) 156, 436
MboII GAAGA 7 cut(s) 352, 773, 781, 784, 787, 908, 916
MhlI GDGCHC 3 cut(s) 111, 166, 279
MluCI AATT 1 cut(s) 721
MlyI GAGTC 2 cut(s) 356, 475
MnlI CCTC 9 cut(s) 67, 112, 174, 202, 414, 501, 625, 666, 710
MseI TTAA 3 cut(s) 405, 656, 731
MslI CAYNNNNRTG 1 cut(s) 853
MspI CCGG 1 cut(s) 779
MwoI GCNNNNNNNGC 7 cut(s) 38, 43, 47, 52, 170, 458, 590
NdeII GATC 2 cut(s) 156, 436
NlaIII CATG 3 cut(s) 304, 664, 852
NlaIV GGNNCC 1 cut(s) 931
NmeAIII GCCGAG 2 cut(s) 67, 76
NmuCI GTSAC 1 cut(s) 575
PagI TCATGA 1 cut(s) 300
PfeI GAWTC 1 cut(s) 635
PflMI CCANNNNNTGG 1 cut(s) 713
PkrI GCNGC 3 cut(s) 463, 650, 889
PleI GAGTC 2 cut(s) 355, 475
PpsI GAGTC 2 cut(s) 355, 475
PsiI TTATAA 1 cut(s) 726
Psp124BI GAGCTC 3 cut(s) 111, 166, 279
PspN4I GGNNCC 1 cut(s) 931
PspPI GGNCC 1 cut(s) 913
PstI CTGCAG 1 cut(s) 892
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
RseI CAYNNNNRTG 1 cut(s) 853
SacI GAGCTC 3 cut(s) 111, 166, 279
SaqAI TTAA 3 cut(s) 405, 656, 731
SatI GCNGC 3 cut(s) 462, 649, 888
Sau3AI GATC 2 cut(s) 156, 436
Sau96I GGNCC 1 cut(s) 913
SchI GAGTC 2 cut(s) 356, 475
SduI GDGCHC 3 cut(s) 111, 166, 279
SfaNI GCATC 2 cut(s) 328, 758
SfcI CTRYAG 1 cut(s) 888
SinI GGWCC 1 cut(s) 913
SmiMI CAYNNNNRTG 1 cut(s) 853
SmlI CTYRAG 2 cut(s) 670, 772
SmoI CTYRAG 2 cut(s) 670, 772
Sse9I AATT 1 cut(s) 721
SsiI CCGC 1 cut(s) 30
SspMI CTAG 2 cut(s) 468, 923
SstI GAGCTC 3 cut(s) 111, 166, 279
TaaI ACNGT 4 cut(s) 115, 189, 307, 355
TaiI ACGT 2 cut(s) 24, 156
TaqI TCGA 3 cut(s) 117, 345, 816
TasI AATT 1 cut(s) 721
TfiI GAWTC 1 cut(s) 635
Tru1I TTAA 3 cut(s) 405, 656, 731
Tru9I TTAA 3 cut(s) 405, 656, 731
TscAI CASTG 3 cut(s) 118, 298, 358
TseFI GTSAC 1 cut(s) 575
TseI GCWGC 3 cut(s) 461, 648, 887
Tsp45I GTSAC 1 cut(s) 575
TspDTI ATGAA 6 cut(s) 17, 78, 386, 561, 615, 837
TspRI CASTG 3 cut(s) 118, 298, 358
Van91I CCANNNNNTGG 1 cut(s) 713
VpaK11BI GGWCC 1 cut(s) 913
XcmI CCANNNNNNNNNTGG 1 cut(s) 923
XmiI GTMKAC 1 cut(s) 24
XspI CTAG 2 cut(s) 468, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.