RchiOBHm_Chr4g0401061

CSC1-like protein At4g02900

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
N/A
Physical Location & Seq
Forward (+)
19049113 .. 19049655
543 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 543 bp
ATGGATCCTGGTTCCTTGGCCTTTACTACAAATGAACCTAGAATACAGTTATATCTGTTGCTGGGGCTTGTATATTATGTTGTCACACCTTTACTTCTTCCATTTATTCTTTTCTTCTTTGCTTTTGCCTGCATGGTTTATAGTCACCAGGTGGGTTATACTACATCTCAGTCAGGCTCAAAATTCTGGTCTATCATCCTCTGCTTATCTCATTGTTTACAAGTAACTCATTGTTTTTGTCCCCTTGTTGCCAGATTATTAATGTCTATGATCAGAAATGAGAGTGCTGCAGCCTTCTGGCCACGTGTGCATATGCGTGTAATTATTGGCCTAATCGTATCCCAATTCCTTGTAATTGGACTATTTAGCACATTAGATGTGCTGTTTTCAACATATATTCTCATTCCACAACCTGTTTTGACATTCTGGTTCCATAGAGTCTGCAAGGGTCGTTTCGAACCAGCATTCGTGAAATTTCCTTTACAGGCCAGAATGAATTTTCTAGTACGAGTTACTTTGGTTATACGTTTACTTCTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

180

Amino Acids

20.69

Weight (kDa)

9.54

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_7TM PF02714 2 - 67 4e-10 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 299
AcsI RAATTY 3 cut(s) 182, 473, 496
AcvI CACGTG 1 cut(s) 305
AdeI CACNNNGTG 1 cut(s) 151
AfaI GTAC 1 cut(s) 507
AflIII ACRYGT 1 cut(s) 304
AgsI TTSAA 1 cut(s) 390
AjnI CCWGG 2 cut(s) 7, 147
AlwI GGATC 1 cut(s) 12
AoxI GGCC 4 cut(s) 18, 299, 328, 486
ApeKI GCWGC 2 cut(s) 287, 290
ApoI RAATTY 3 cut(s) 182, 473, 496
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
AseI ATTAAT 1 cut(s) 260
AsuHPI GGTGA 1 cut(s) 137
AsuII TTCGAA 1 cut(s) 456
BalI TGGCCA 1 cut(s) 301
BamHI GGATCC 1 cut(s) 4
BbrPI CACGTG 1 cut(s) 305
BbvI GCAGC 2 cut(s) 274, 302
BciT130I CCWGG 2 cut(s) 9, 149
BciVI GTATCC 1 cut(s) 349
BclI TGATCA 1 cut(s) 270
BfaI CTAG 2 cut(s) 39, 503
BfmI CTRYAG 1 cut(s) 288
BfuI GTATCC 1 cut(s) 349
BisI GCNGC 2 cut(s) 288, 291
BlsI GCNGC 2 cut(s) 289, 292
Bme1390I CCNGG 2 cut(s) 9, 149
BmiI GGNNCC 3 cut(s) 6, 13, 431
BmrFI CCNGG 2 cut(s) 9, 149
Bpu14I TTCGAA 1 cut(s) 456
BsaAI YACGTR 1 cut(s) 305
BsaJI CCNNGG 1 cut(s) 15
BseBI CCWGG 2 cut(s) 9, 149
BseDI CCNNGG 1 cut(s) 15
BseGI GGATG 1 cut(s) 195
BseMII CTCAG 1 cut(s) 182
BseXI GCAGC 2 cut(s) 274, 302
BseYI CCCAGC 1 cut(s) 61
BshFI GGCC 4 cut(s) 20, 301, 330, 488
BslFI GGGAC 1 cut(s) 225
BsmFI GGGAC 1 cut(s) 225
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 4 cut(s) 20, 301, 330, 488
Bsp119I TTCGAA 1 cut(s) 456
Bsp143I GATC 2 cut(s) 4, 270
BspANI GGCC 4 cut(s) 20, 301, 330, 488
BspCNI CTCAG 1 cut(s) 181
BspLI GGNNCC 3 cut(s) 6, 13, 431
BspMAI CTGCAG 1 cut(s) 292
BspPI GGATC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 456
BssECI CCNNGG 1 cut(s) 15
BssMI GATC 2 cut(s) 4, 270
BssT1I CCWWGG 1 cut(s) 15
Bst2UI CCWGG 2 cut(s) 9, 149
Bst4CI ACNGT 1 cut(s) 48
BstBAI YACGTR 1 cut(s) 305
BstBI TTCGAA 1 cut(s) 456
BstC8I GCNNGC 1 cut(s) 130
BstDEI CTNAG 1 cut(s) 168
BstF5I GGATG 1 cut(s) 195
BstKTI GATC 2 cut(s) 7, 273
BstMBI GATC 2 cut(s) 4, 270
BstMWI GCNNNNNNNGC 1 cut(s) 307
BstNI CCWGG 2 cut(s) 9, 149
BstSCI CCNGG 2 cut(s) 7, 147
BstSFI CTRYAG 1 cut(s) 288
BstV1I GCAGC 2 cut(s) 274, 302
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuI GTATCC 1 cut(s) 349
BsuRI GGCC 4 cut(s) 20, 301, 330, 488
BtsCI GGATG 1 cut(s) 195
Cac8I GCNNGC 1 cut(s) 130
CsiI ACCWGGT 1 cut(s) 147
Csp6I GTAC 1 cut(s) 506
CviAII CATG 1 cut(s) 133
CviJI RGCY 7 cut(s) 20, 67, 177, 293, 301, 330, 488
CviKI_1 RGCY 7 cut(s) 20, 67, 177, 293, 301, 330, 488
CviQI GTAC 1 cut(s) 506
DdeI CTNAG 1 cut(s) 168
DpnI GATC 2 cut(s) 6, 272
DpnII GATC 2 cut(s) 4, 270
DraIII CACNNNGTG 1 cut(s) 151
EaeI YGGCCR 1 cut(s) 299
Eco130I CCWWGG 1 cut(s) 15
Eco72I CACGTG 1 cut(s) 305
EcoRII CCWGG 2 cut(s) 7, 147
EcoT14I CCWWGG 1 cut(s) 15
ErhI CCWWGG 1 cut(s) 15
FaeI CATG 1 cut(s) 136
FaqI GGGAC 1 cut(s) 225
FatI CATG 1 cut(s) 132
FauNDI CATATG 1 cut(s) 312
FbaI TGATCA 1 cut(s) 270
Fnu4HI GCNGC 2 cut(s) 288, 291
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 2 cut(s) 288, 291
FspBI CTAG 2 cut(s) 39, 503
GluI GCNGC 2 cut(s) 288, 291
GsaI CCCAGC 1 cut(s) 65
HaeIII GGCC 4 cut(s) 20, 301, 330, 488
Hin1II CATG 1 cut(s) 136
HinfI GANTC 1 cut(s) 438
HphI GGTGA 1 cut(s) 137
Hpy166II GTNNAC 2 cut(s) 218, 530
Hpy188I TCNGA 1 cut(s) 275
Hpy188III TCNNGA 1 cut(s) 469
Hpy8I GTNNAC 2 cut(s) 218, 530
HpyAV CCTTC 1 cut(s) 304
HpyCH4III ACNGT 1 cut(s) 48
HpyCH4IV ACGT 2 cut(s) 304, 526
HpyCH4V TGCA 4 cut(s) 132, 290, 310, 444
HpyF10VI GCNNNNNNNGC 1 cut(s) 307
HpyF3I CTNAG 1 cut(s) 168
HpySE526I ACGT 2 cut(s) 304, 526
Hsp92II CATG 1 cut(s) 136
Ksp22I TGATCA 1 cut(s) 270
Kzo9I GATC 2 cut(s) 4, 270
Lsp1109I GCAGC 2 cut(s) 274, 302
MabI ACCWGGT 1 cut(s) 147
MaeI CTAG 2 cut(s) 39, 503
MaeII ACGT 2 cut(s) 304, 526
MaeIII GTNAC 4 cut(s) 82, 143, 223, 511
MalI GATC 2 cut(s) 6, 272
MboI GATC 2 cut(s) 4, 270
MboII GAAGA 2 cut(s) 89, 106
MflI RGATCY 1 cut(s) 4
MlsI TGGCCA 1 cut(s) 301
MluCI AATT 6 cut(s) 182, 321, 344, 354, 473, 496
MluNI TGGCCA 1 cut(s) 301
MlyI GAGTC 1 cut(s) 447
MnlI CCTC 1 cut(s) 209
Mox20I TGGCCA 1 cut(s) 301
MscI TGGCCA 1 cut(s) 301
MseI TTAA 2 cut(s) 260, 541
MslI CAYNNNNRTG 1 cut(s) 315
Msp20I TGGCCA 1 cut(s) 301
MspR9I CCNGG 2 cut(s) 9, 149
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 2 cut(s) 9, 149
MwoI GCNNNNNNNGC 1 cut(s) 307
NdeI CATATG 1 cut(s) 312
NdeII GATC 2 cut(s) 4, 270
NlaIII CATG 1 cut(s) 136
NlaIV GGNNCC 3 cut(s) 6, 13, 431
NmuCI GTSAC 2 cut(s) 82, 143
NspV TTCGAA 1 cut(s) 456
PctI GAATGC 1 cut(s) 464
PkrI GCNGC 2 cut(s) 289, 292
PleI GAGTC 1 cut(s) 446
PmaCI CACGTG 1 cut(s) 305
PmlI CACGTG 1 cut(s) 305
PpsI GAGTC 1 cut(s) 446
Ppu21I YACGTR 1 cut(s) 305
PshBI ATTAAT 1 cut(s) 260
Psp6I CCWGG 2 cut(s) 7, 147
PspCI CACGTG 1 cut(s) 305
PspFI CCCAGC 1 cut(s) 61
PspGI CCWGG 2 cut(s) 7, 147
PspN4I GGNNCC 3 cut(s) 6, 13, 431
PsrI GAACNNNNNNTAC 2 cut(s) 27, 59
PstI CTGCAG 1 cut(s) 292
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 1 cut(s) 507
RsaNI GTAC 1 cut(s) 506
RseI CAYNNNNRTG 1 cut(s) 315
SaqAI TTAA 2 cut(s) 260, 541
SatI GCNGC 2 cut(s) 288, 291
Sau3AI GATC 2 cut(s) 4, 270
SchI GAGTC 1 cut(s) 447
ScrFI CCNGG 2 cut(s) 9, 149
SetI ASST 6 cut(s) 40, 91, 153, 307, 415, 529
SexAI ACCWGGT 1 cut(s) 147
SfcI CTRYAG 1 cut(s) 288
SfuI TTCGAA 1 cut(s) 456
SmiMI CAYNNNNRTG 1 cut(s) 315
Sse9I AATT 6 cut(s) 182, 321, 344, 354, 473, 496
SspMI CTAG 2 cut(s) 39, 503
StyD4I CCNGG 2 cut(s) 7, 147
StyI CCWWGG 1 cut(s) 15
TaaI ACNGT 1 cut(s) 48
TaiI ACGT 2 cut(s) 307, 529
TaqI TCGA 1 cut(s) 456
TasI AATT 6 cut(s) 182, 321, 344, 354, 473, 496
Tru1I TTAA 2 cut(s) 260, 541
Tru9I TTAA 2 cut(s) 260, 541
TseFI GTSAC 2 cut(s) 82, 143
TseI GCWGC 2 cut(s) 287, 290
Tsp45I GTSAC 2 cut(s) 82, 143
TspDTI ATGAA 2 cut(s) 48, 509
VspI ATTAAT 1 cut(s) 260
XapI RAATTY 3 cut(s) 182, 473, 496
XspI CTAG 2 cut(s) 39, 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.