RchiOBHm_Chr4g0402801

nicotianamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
N/A
Physical Location & Seq
Forward (+)
21656476 .. 21656685
210 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 210 bp
ATGGAATCCCAAATTCCTGCTGAGCTTCTCAAAACTCACATTATGCAACTCCATGCTAGCATATCCAAGATCGACTCCCTTCGCCCTTCAAAGCAAGTCAACTCCCTCTTCACTCAGCTGGTCAAGTTGTGCACTCTTCCATCTTCGGTGGATGTTCATGACTTATCCGAAGACGTCCAAATTATGCGCGAGAGTCTCATCAATCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAS

Protein Analysis

69

Amino Acids

7.77

Weight (kDa)

5.8

Isoelectric Point (pI)

49.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAS PF03059 8 - 69 3.3e-14 Nicotianamine synthase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 177
AccII CGCG 1 cut(s) 189
AcsI RAATTY 1 cut(s) 12
AcyI GRCGYC 1 cut(s) 174
AgsI TTSAA 1 cut(s) 90
AluBI AGCT 2 cut(s) 25, 118
AluI AGCT 2 cut(s) 25, 118
Alw21I GWGCWC 1 cut(s) 134
Alw26I GTCTC 1 cut(s) 200
Alw44I GTGCAC 1 cut(s) 130
ApaLI GTGCAC 1 cut(s) 130
ApoI RAATTY 1 cut(s) 12
AspLEI GCGC 1 cut(s) 189
AsuNHI GCTAGC 1 cut(s) 56
BaeGI GKGCMC 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 177
Bbv12I GWGCWC 1 cut(s) 134
BccI CCATC 1 cut(s) 148
BcoDI GTCTC 1 cut(s) 200
BfaI CTAG 1 cut(s) 57
BlpI GCTNAGC 1 cut(s) 21
BmtI GCTAGC 1 cut(s) 60
BpiI GAAGAC 1 cut(s) 177
Bpu1102I GCTNAGC 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 174
BseGI GGATG 1 cut(s) 157
BseMII CTCAG 2 cut(s) 12, 128
BseSI GKGCMC 1 cut(s) 134
Bsh1236I CGCG 1 cut(s) 189
BsiHKAI GWGCWC 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 200
Bsp1286I GDGCHC 1 cut(s) 134
Bsp143I GATC 1 cut(s) 69
Bsp1720I GCTNAGC 1 cut(s) 21
BspCNI CTCAG 2 cut(s) 13, 127
BspFNI CGCG 1 cut(s) 189
BspHI TCATGA 1 cut(s) 157
BspOI GCTAGC 1 cut(s) 60
BssMI GATC 1 cut(s) 69
BssNI GRCGYC 1 cut(s) 174
Bst6I CTCTTC 2 cut(s) 113, 141
BstACI GRCGYC 1 cut(s) 174
BstC8I GCNNGC 1 cut(s) 58
BstDEI CTNAG 2 cut(s) 21, 114
BstF5I GGATG 1 cut(s) 157
BstFNI CGCG 1 cut(s) 189
BstHHI GCGC 1 cut(s) 189
BstKTI GATC 1 cut(s) 72
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 1 cut(s) 69
BstSLI GKGCMC 1 cut(s) 134
BstUI CGCG 1 cut(s) 189
BstV2I GAAGAC 1 cut(s) 177
BtsCI GGATG 1 cut(s) 157
Cac8I GCNNGC 1 cut(s) 58
CciI TCATGA 1 cut(s) 157
CfoI GCGC 1 cut(s) 189
CviAII CATG 2 cut(s) 53, 158
CviJI RGCY 2 cut(s) 25, 118
CviKI_1 RGCY 2 cut(s) 25, 118
DdeI CTNAG 2 cut(s) 21, 114
DpnI GATC 1 cut(s) 71
DpnII GATC 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 113, 141
EarI CTCTTC 2 cut(s) 113, 141
FaeI CATG 2 cut(s) 56, 161
FaiI YATR 5 cut(s) 44, 54, 62, 159, 185
FatI CATG 2 cut(s) 52, 157
FokI GGATG 1 cut(s) 164
FspBI CTAG 1 cut(s) 57
GlaI GCGC 1 cut(s) 188
HhaI GCGC 1 cut(s) 189
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 2 cut(s) 56, 161
Hin6I GCGC 1 cut(s) 187
HinP1I GCGC 1 cut(s) 187
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 3 cut(s) 5, 74, 193
Hpy166II GTNNAC 2 cut(s) 100, 132
Hpy188I TCNGA 2 cut(s) 169, 209
Hpy188III TCNNGA 1 cut(s) 158
Hpy8I GTNNAC 2 cut(s) 100, 132
HpyAV CCTTC 2 cut(s) 89, 96
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 2 cut(s) 46, 132
HpyF3I CTNAG 2 cut(s) 21, 114
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 2 cut(s) 56, 161
HspAI GCGC 1 cut(s) 187
Kzo9I GATC 1 cut(s) 69
LpnPI CCDG 2 cut(s) 30, 104
MaeI CTAG 1 cut(s) 57
MaeII ACGT 1 cut(s) 174
MalI GATC 1 cut(s) 71
MboI GATC 1 cut(s) 69
MboII GAAGA 4 cut(s) 100, 128, 135, 182
MhlI GDGCHC 1 cut(s) 134
MluCI AATT 2 cut(s) 12, 180
MlyI GAGTC 2 cut(s) 68, 202
MnlI CCTC 1 cut(s) 116
MspA1I CMGCKG 1 cut(s) 118
MvnI CGCG 1 cut(s) 189
NdeII GATC 1 cut(s) 69
NheI GCTAGC 1 cut(s) 56
NlaIII CATG 2 cut(s) 56, 161
PagI TCATGA 1 cut(s) 157
PfeI GAWTC 1 cut(s) 5
PleI GAGTC 2 cut(s) 68, 201
PpsI GAGTC 2 cut(s) 68, 201
PvuII CAGCTG 1 cut(s) 118
Sau3AI GATC 1 cut(s) 69
SchI GAGTC 2 cut(s) 68, 202
SduI GDGCHC 1 cut(s) 134
SetI ASST 3 cut(s) 27, 120, 177
Sse9I AATT 2 cut(s) 12, 180
SspMI CTAG 1 cut(s) 57
TaiI ACGT 1 cut(s) 177
TaqI TCGA 1 cut(s) 72
TasI AATT 2 cut(s) 12, 180
TfiI GAWTC 1 cut(s) 5
TspDTI ATGAA 1 cut(s) 146
VneI GTGCAC 1 cut(s) 130
XapI RAATTY 1 cut(s) 12
XspI CTAG 1 cut(s) 57
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.