RchiOBHm_Chr4g0419431

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
N/A
Physical Location & Seq
Reverse (-)
44848850 .. 44849762
913 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 864 bp
ATGGTGAAACTCTCTAGATGTACTGCTCCCCCTCTTTGTAATTTGGCCTTTGACATTGCTACTGTTTTGCGCTTAGTTGTGACTGAAGAAGATCATCTTCTGTTGGATATGATTTCATTGGTTGGGCAAGGGGAAGCCGATGATAAACCATCTTTGTGTCTATTTGAGCAGAACCTTGTTATACAGACTAGTTTGGTTGACTTCTATGCAAGAACTGGGTGTGTGGACACTGCACGCCAAGTGATCGATAGAATTCCCCAACCAGACTTGGTTCCTTGGAATGCATTGATAGCTGGTTATTCGTCGAATGGATTCGATTGGGAAGCATTAGAGGTTTTTAGGGAGATTGTTTTCAGGTGTGTTCATGCTAGTAAGTCTCTTCATTGCTTTGCTGTCAAGTTTGGATACTTTTCGAATGATTTTCTGGTTCCTGCTTTGATTTCAATGTACGCAGGGGATGGGGATACATGTGGTGCTAGAAAGTTGTTTGATTCTGTAGTGGAAAAGAATGTTGCTGTTTGGAATGCCATGATTTCTGCATATACACAAAGGCAAGAGCCTGTGTTAGCTTTTGAGTTGTTCCGGTCGATGCTGCTAGTTGACATAAGGCCTAATTTGGTCACTTTCATGTCGATTATTCCCTCATTTGAGAATTTCATTAGCCTTTCTTTTGGCGAGTCTCTTCATGCTTGTGTAGTTAAACATGGATCAGAAAATCAGATTCCGGTGTTAACTGCCCTGGTATCAATGTATGCCAAATTTGGTAGTATTGATGAATCCAGGTATCTTTTCAACCAGATGCAAAGTAAAAACCTTCTTTTGTGGAATTCAATGATTTCTGGATACGTGTACAATGGGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 WEMBL

Protein Analysis

287

Amino Acids

32.16

Weight (kDa)

5.02

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 169 - 213 5.2e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 715
AcsI RAATTY 4 cut(s) 252, 652, 758, 826
AcuI CTGAAG 1 cut(s) 105
AfaI GTAC 3 cut(s) 22, 449, 851
AflIII ACRYGT 2 cut(s) 467, 846
AgsI TTSAA 3 cut(s) 444, 793, 831
AhlI ACTAGT 1 cut(s) 188
AjnI CCWGG 2 cut(s) 738, 779
AluBI AGCT 2 cut(s) 293, 569
AluI AGCT 2 cut(s) 293, 569
Alw26I GTCTC 2 cut(s) 381, 684
AlwI GGATC 1 cut(s) 715
AoxI GGCC 2 cut(s) 45, 608
ApeKI GCWGC 1 cut(s) 592
ApoI RAATTY 4 cut(s) 252, 652, 758, 826
Asp700I GAANNNNTTC 1 cut(s) 311
AspLEI GCGC 1 cut(s) 72
AsuHPI GGTGA 1 cut(s) 16
AsuII TTCGAA 1 cut(s) 413
BbvI GCAGC 1 cut(s) 579
BccI CCATC 2 cut(s) 157, 452
BciT130I CCWGG 2 cut(s) 740, 781
BciVI GTATCC 3 cut(s) 398, 457, 836
BcoDI GTCTC 2 cut(s) 381, 684
BcuI ACTAGT 1 cut(s) 188
BfaI CTAG 5 cut(s) 15, 189, 369, 477, 596
BfmI CTRYAG 1 cut(s) 495
BfuI GTATCC 3 cut(s) 398, 457, 836
BisI GCNGC 1 cut(s) 593
BlsI GCNGC 1 cut(s) 594
Bme1390I CCNGG 2 cut(s) 740, 781
BmiI GGNNCC 2 cut(s) 273, 429
BmrFI CCNGG 2 cut(s) 740, 781
BmrI ACTGGG 1 cut(s) 225
BmsI GCATC 2 cut(s) 579, 789
BmuI ACTGGG 1 cut(s) 225
Bpu14I TTCGAA 1 cut(s) 413
Bsa29I ATCGAT 1 cut(s) 246
BsaAI YACGTR 1 cut(s) 847
BsaJI CCNNGG 2 cut(s) 275, 738
BsaWI WCCGGW 2 cut(s) 582, 724
Bse1I ACTGG 1 cut(s) 220
Bse3DI GCAATG 2 cut(s) 54, 382
BseBI CCWGG 2 cut(s) 740, 781
BseCI ATCGAT 1 cut(s) 246
BseDI CCNNGG 2 cut(s) 275, 738
BseGI GGATG 1 cut(s) 463
BseMI GCAATG 2 cut(s) 54, 382
BseNI ACTGG 1 cut(s) 220
BseXI GCAGC 1 cut(s) 579
BsgI GTGCAG 1 cut(s) 216
Bsh1285I CGRYCG 1 cut(s) 587
BshFI GGCC 2 cut(s) 47, 610
BshVI ATCGAT 1 cut(s) 246
BsiEI CGRYCG 1 cut(s) 587
BsiSI CCGG 2 cut(s) 583, 725
BsmAI GTCTC 2 cut(s) 381, 684
BsmI GAATGC 2 cut(s) 286, 529
BsnI GGCC 2 cut(s) 47, 610
Bsp119I TTCGAA 1 cut(s) 413
Bsp1407I TGTACA 1 cut(s) 849
Bsp143I GATC 3 cut(s) 91, 243, 707
BspANI GGCC 2 cut(s) 47, 610
BspDI ATCGAT 1 cut(s) 246
BspLI GGNNCC 2 cut(s) 273, 429
BspPI GGATC 1 cut(s) 715
BspT104I TTCGAA 1 cut(s) 413
BsrDI GCAATG 2 cut(s) 54, 382
BsrGI TGTACA 1 cut(s) 849
BsrI ACTGG 1 cut(s) 220
BssECI CCNNGG 2 cut(s) 275, 738
BssMI GATC 3 cut(s) 91, 243, 707
BssT1I CCWWGG 1 cut(s) 275
Bst2UI CCWGG 2 cut(s) 740, 781
Bst4CI ACNGT 1 cut(s) 64
Bst6I CTCTTC 2 cut(s) 384, 687
BstAUI TGTACA 1 cut(s) 849
BstBAI YACGTR 1 cut(s) 847
BstBI TTCGAA 1 cut(s) 413
BstC8I GCNNGC 1 cut(s) 235
BstDEI CTNAG 1 cut(s) 73
BstF5I GGATG 1 cut(s) 463
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 3 cut(s) 94, 246, 710
BstMAI GTCTC 2 cut(s) 381, 684
BstMBI GATC 3 cut(s) 91, 243, 707
BstMCI CGRYCG 1 cut(s) 587
BstMWI GCNNNNNNNGC 1 cut(s) 290
BstNI CCWGG 2 cut(s) 740, 781
BstNSI RCATGY 1 cut(s) 471
BstSCI CCNGG 2 cut(s) 738, 779
BstSFI CTRYAG 1 cut(s) 495
BstV1I GCAGC 1 cut(s) 579
Bsu15I ATCGAT 1 cut(s) 246
BsuI GTATCC 3 cut(s) 398, 457, 836
BsuRI GGCC 2 cut(s) 47, 610
BsuTUI ATCGAT 1 cut(s) 246
BtsCI GGATG 1 cut(s) 463
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 1 cut(s) 228
Cac8I GCNNGC 1 cut(s) 235
CfoI GCGC 1 cut(s) 72
ClaI ATCGAT 1 cut(s) 246
Csp6I GTAC 3 cut(s) 21, 448, 850
CviAII CATG 6 cut(s) 365, 468, 529, 628, 686, 704
CviJI RGCY 7 cut(s) 47, 137, 293, 559, 569, 610, 663
CviKI_1 RGCY 7 cut(s) 47, 137, 293, 559, 569, 610, 663
CviQI GTAC 3 cut(s) 21, 448, 850
DdeI CTNAG 1 cut(s) 73
DpnI GATC 3 cut(s) 93, 245, 709
DpnII GATC 3 cut(s) 91, 243, 707
Eam1104I CTCTTC 2 cut(s) 384, 687
EarI CTCTTC 2 cut(s) 384, 687
Eco130I CCWWGG 1 cut(s) 275
Eco147I AGGCCT 1 cut(s) 610
Eco57I CTGAAG 1 cut(s) 105
EcoRI GAATTC 2 cut(s) 252, 826
EcoRII CCWGG 2 cut(s) 738, 779
EcoT14I CCWWGG 1 cut(s) 275
EcoT22I ATGCAT 1 cut(s) 286
ErhI CCWWGG 1 cut(s) 275
FaeI CATG 6 cut(s) 368, 471, 532, 631, 689, 707
FalI AAGNNNNNCTT 2 cut(s) 81, 113
FatI CATG 6 cut(s) 364, 467, 528, 627, 685, 703
Fnu4HI GCNGC 1 cut(s) 593
FokI GGATG 1 cut(s) 470
Fsp4HI GCNGC 1 cut(s) 593
FspBI CTAG 5 cut(s) 15, 189, 369, 477, 596
GlaI GCGC 1 cut(s) 71
GluI GCNGC 1 cut(s) 593
HaeIII GGCC 2 cut(s) 47, 610
HapII CCGG 2 cut(s) 583, 725
HhaI GCGC 1 cut(s) 72
Hin1II CATG 6 cut(s) 368, 471, 532, 631, 689, 707
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HincII GTYRAC 3 cut(s) 199, 601, 732
HindII GTYRAC 3 cut(s) 199, 601, 732
HinfI GANTC 5 cut(s) 312, 491, 677, 721, 776
HpaI GTTAAC 1 cut(s) 732
HpaII CCGG 2 cut(s) 583, 725
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 5 cut(s) 199, 226, 601, 732, 850
Hpy188I TCNGA 2 cut(s) 712, 720
Hpy188III TCNNGA 2 cut(s) 15, 840
Hpy8I GTNNAC 5 cut(s) 199, 226, 601, 732, 850
Hpy99I CGWCG 1 cut(s) 307
HpyAV CCTTC 1 cut(s) 824
HpyCH4III ACNGT 1 cut(s) 64
HpyCH4IV ACGT 1 cut(s) 846
HpyCH4V TGCA 5 cut(s) 209, 233, 284, 539, 802
HpyF10VI GCNNNNNNNGC 1 cut(s) 290
HpyF3I CTNAG 1 cut(s) 73
HpySE526I ACGT 1 cut(s) 846
Hsp92II CATG 6 cut(s) 368, 471, 532, 631, 689, 707
HspAI GCGC 1 cut(s) 70
KspAI GTTAAC 1 cut(s) 732
Kzo9I GATC 3 cut(s) 91, 243, 707
LmnI GCTCC 1 cut(s) 31
Lsp1109I GCAGC 1 cut(s) 579
LweI GCATC 2 cut(s) 579, 789
MaeI CTAG 5 cut(s) 15, 189, 369, 477, 596
MaeII ACGT 1 cut(s) 846
MaeIII GTNAC 2 cut(s) 79, 619
MalI GATC 3 cut(s) 93, 245, 709
MboI GATC 3 cut(s) 91, 243, 707
MboII GAAGA 5 cut(s) 89, 98, 101, 371, 674
MluCI AATT 6 cut(s) 40, 252, 613, 652, 758, 826
MlyI GAGTC 1 cut(s) 686
MmeI TCCRAC 1 cut(s) 84
MnlI CCTC 3 cut(s) 42, 325, 652
Mph1103I ATGCAT 1 cut(s) 286
MroXI GAANNNNTTC 1 cut(s) 311
MseI TTAA 2 cut(s) 699, 731
MslI CAYNNNNRTG 3 cut(s) 154, 626, 690
MspI CCGG 2 cut(s) 583, 725
MspR9I CCNGG 2 cut(s) 740, 781
Mva1269I GAATGC 2 cut(s) 286, 529
MvaI CCWGG 2 cut(s) 740, 781
MwoI GCNNNNNNNGC 1 cut(s) 290
NdeII GATC 3 cut(s) 91, 243, 707
NlaIII CATG 6 cut(s) 368, 471, 532, 631, 689, 707
NlaIV GGNNCC 2 cut(s) 273, 429
NmuCI GTSAC 2 cut(s) 79, 619
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 1 cut(s) 471
NspV TTCGAA 1 cut(s) 413
PceI AGGCCT 1 cut(s) 610
PciI ACATGT 1 cut(s) 467
PctI GAATGC 2 cut(s) 286, 529
PdmI GAANNNNTTC 1 cut(s) 311
PfeI GAWTC 4 cut(s) 312, 491, 721, 776
PkrI GCNGC 1 cut(s) 594
PleI GAGTC 1 cut(s) 685
PpsI GAGTC 1 cut(s) 685
Ppu21I YACGTR 1 cut(s) 847
PscI ACATGT 1 cut(s) 467
Psp6I CCWGG 2 cut(s) 738, 779
PspGI CCWGG 2 cut(s) 738, 779
PspN4I GGNNCC 2 cut(s) 273, 429
RsaI GTAC 3 cut(s) 22, 449, 851
RsaNI GTAC 3 cut(s) 21, 448, 850
RseI CAYNNNNRTG 3 cut(s) 154, 626, 690
SaqAI TTAA 2 cut(s) 699, 731
SatI GCNGC 1 cut(s) 593
Sau3AI GATC 3 cut(s) 91, 243, 707
SchI GAGTC 1 cut(s) 686
ScrFI CCNGG 2 cut(s) 740, 781
SetI ASST 8 cut(s) 177, 295, 336, 359, 571, 785, 816, 849
SfaNI GCATC 2 cut(s) 579, 789
SfcI CTRYAG 1 cut(s) 495
SfuI TTCGAA 1 cut(s) 413
SmiMI CAYNNNNRTG 3 cut(s) 154, 626, 690
SpeI ACTAGT 1 cut(s) 188
Sse9I AATT 6 cut(s) 40, 252, 613, 652, 758, 826
SseBI AGGCCT 1 cut(s) 610
SspMI CTAG 5 cut(s) 15, 189, 369, 477, 596
StuI AGGCCT 1 cut(s) 610
StyD4I CCNGG 2 cut(s) 738, 779
StyI CCWWGG 1 cut(s) 275
TaaI ACNGT 1 cut(s) 64
TaiI ACGT 1 cut(s) 849
TaqI TCGA 6 cut(s) 246, 305, 315, 413, 587, 632
TasI AATT 6 cut(s) 40, 252, 613, 652, 758, 826
TatI WGTACW 2 cut(s) 20, 849
TfiI GAWTC 4 cut(s) 312, 491, 721, 776
Tru1I TTAA 2 cut(s) 699, 731
Tru9I TTAA 2 cut(s) 699, 731
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 2 cut(s) 79, 619
TseI GCWGC 1 cut(s) 592
Tsp45I GTSAC 2 cut(s) 79, 619
TspDTI ATGAA 7 cut(s) 105, 353, 371, 616, 646, 674, 789
TspRI CASTG 1 cut(s) 235
XapI RAATTY 4 cut(s) 252, 652, 758, 826
XbaI TCTAGA 1 cut(s) 14
XceI RCATGY 1 cut(s) 471
XmnI GAANNNNTTC 1 cut(s) 311
XspI CTAG 5 cut(s) 15, 189, 369, 477, 596
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.