RchiOBHm_Chr4g0437701

Cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
N/A
Physical Location & Seq
Reverse (-)
60650312 .. 60652820
2509 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1353 bp
ATGAGGTCTTCAGGAGGCCTACTGATCATTATTTCAAGTTTTCTGTGTCTTTTCTGTTTTGTAGCTCTGCTCTTTAAGATCTTTCACAAAATATGGTGGACTCCGAATTGCATACAGAAGTTGATGGCTTCACAGGGAATCAGAGGCCCTCCTTACAGACTTATCCATGGAAACACAAAAGAAATCTCCAACATGTACAAGGAAGCCAGGAGCAGACCCTTAAGTTTATCACATGACATATTTTCTTATGTTCAGTCTCATGTTCACTCATATATGGAGCAATATATTTTCTTAAGTAGTGTTTATCGCTTCGAGCCCCATGAATATCCGGATGGCATTTCCAAACTTGAAACTATGAGTATGATCCTCAATGAGTCTCTAAGGTTATATCCTCCTGCTGTTGCCATTCCACGGATCACTGGAAGGGTAGTTAGACTAGGAAAGCTCATTGTTCCTGCTAATGTTCAACTGCTGGTCTCAGTTCTAGCACCTCACCATGAACCTCAATTTTGGGGACCAGATGTGCATCTTTTCAAGCCTGAGAGATTCTCTGGAGGGGTTGCTAAAGCTACTAACAATAATGTAGGTGCTTTTGTGCCGTTTGGACTGGGACCTCGAACTTGTGTGGGCATGAACTTTGCAACCACTGAAGCTAAGATTGCCTTGTCGTTGATTCTACAACGCTCCTCCTTCACTCTCTCCCCGGCTTATATCCACGCCCCCTATCAGTCTATCATATTTCGGCCACAACATCGACTTCAAGGCTGTCGGAGACTTGGAGCTCGATCGCCAGAAAACGAAAAGAGAGCTTCGACATCACTATTGAAAAGGTTAAAGGTGAAAGCTTTTTCCAATTATGGAAGACCCAGTAAAACCGAGATCTTTCACAAAATATGGTGGACTCCGATTCGCGTACAGAGACTGATGGCTTCACAAGGAATCAGAGGACCTCCTTACAGACTTATCGATGGAAACGCCAAAGAAGTCTCCAACATGGGCAAGGAAGTCATGAGCAGGCCCTTGGATCTGTCACACAACATATTTCCAGTAGTTCTACCTCATATTCATGCATGGACCAAAATCTATGGGAAGAATTATCTTCAGTGGCATGGTACTCTACCTCAGTTGGTGGTGACGGAAGCTGACTTGTGCAAGGAGATATTCACTAACAAAGATGGAGTTTATCTGAAACCCAAGTTCAGACCTTATGCAAAGAAGGTATTTGGGAATGGCCTTCCGGCATTAGAAGGTGAGAAATGGGTGAAATCGCGAAGGCTGGCCAACCATGCCTTCCGTGGAGAGAGCTTAAACGTAAGTTGTTGCAGTAGTTATGTTAGAAACTGGAGTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00905 map00905
Pfam Domains
Protein Families
p450

Protein Analysis

450

Amino Acids

51.22

Weight (kDa)

10.03

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 113 - 235 2.2e-27 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1351
AccII CGCG 2 cut(s) 912, 1270
AccIII TCCGGA 1 cut(s) 328
AclWI GGATC 3 cut(s) 358, 422, 1032
AcoI YGGCCR 2 cut(s) 743, 1278
AcuI CTGAAG 2 cut(s) 669, 1085
AfaI GTAC 3 cut(s) 197, 915, 1114
AfiI CCNNNNNNNGG 1 cut(s) 411
AflII CTTAAG 2 cut(s) 220, 292
AflIII ACRYGT 1 cut(s) 192
AgsI TTSAA 6 cut(s) 36, 350, 467, 535, 761, 826
AjnI CCWGG 1 cut(s) 206
AjuI GAANNNNNNNTTGG 2 cut(s) 1274, 1306
AluBI AGCT 9 cut(s) 65, 445, 569, 653, 782, 809, 845, 1142, 1305
AluI AGCT 9 cut(s) 65, 445, 569, 653, 782, 809, 845, 1142, 1305
Alw21I GWGCWC 1 cut(s) 784
Alw26I GTCTC 6 cut(s) 261, 381, 481, 766, 913, 991
AlwI GGATC 3 cut(s) 358, 422, 1032
AlwNI CAGNNNCTG 1 cut(s) 922
Aor13HI TCCGGA 1 cut(s) 328
AoxI GGCC 6 cut(s) 16, 145, 743, 1016, 1231, 1278
AspS9I GGNCC 6 cut(s) 146, 515, 611, 947, 1017, 1074
AsuC2I CCSGG 1 cut(s) 704
AsuHPI GGTGA 5 cut(s) 485, 850, 1144, 1262, 1273
AvaII GGWCC 4 cut(s) 515, 611, 947, 1074
BalI TGGCCA 1 cut(s) 1280
BanII GRGCYC 2 cut(s) 318, 784
BbsI GAAGAC 1 cut(s) 868
Bbv12I GWGCWC 1 cut(s) 784
BccI CCATC 5 cut(s) 118, 326, 919, 962, 1169
BceAI ACGGC 1 cut(s) 583
BciT130I CCWGG 1 cut(s) 208
BclI TGATCA 1 cut(s) 24
BcnI CCSGG 1 cut(s) 704
BcoDI GTCTC 6 cut(s) 261, 381, 481, 766, 913, 991
BfaI CTAG 2 cut(s) 437, 485
BfrI CTTAAG 2 cut(s) 220, 292
BglII AGATCT 2 cut(s) 78, 879
Bme1390I CCNGG 2 cut(s) 208, 704
Bme18I GGWCC 4 cut(s) 515, 611, 947, 1074
BmgT120I GGNCC 6 cut(s) 146, 515, 611, 947, 1017, 1074
BmiI GGNNCC 2 cut(s) 516, 612
BmrFI CCNGG 2 cut(s) 208, 704
BmrI ACTGGG 2 cut(s) 617, 861
BmsI GCATC 1 cut(s) 535
BmuI ACTGGG 2 cut(s) 617, 861
BpiI GAAGAC 1 cut(s) 868
BplI GAGNNNNNCTC 2 cut(s) 533, 565
BpmI CTGGAG 1 cut(s) 573
BpuMI CCSGG 1 cut(s) 704
Bsa29I ATCGAT 1 cut(s) 966
BsaBI GATNNNNATC 1 cut(s) 525
BsaI GGTCTC 1 cut(s) 481
BsaJI CCNNGG 5 cut(s) 166, 410, 702, 1020, 1294
BsaWI WCCGGW 1 cut(s) 328
Bsc4I CCNNNNNNNGG 1 cut(s) 411
Bse1I ACTGG 5 cut(s) 424, 612, 867, 1046, 1346
Bse8I GATNNNNATC 1 cut(s) 525
BseAI TCCGGA 1 cut(s) 328
BseBI CCWGG 1 cut(s) 208
BseCI ATCGAT 1 cut(s) 966
BseDI CCNNGG 5 cut(s) 166, 410, 702, 1020, 1294
BseGI GGATG 1 cut(s) 337
BseJI GATNNNNATC 1 cut(s) 525
BseLI CCNNNNNNNGG 1 cut(s) 411
BseMII CTCAG 3 cut(s) 492, 531, 1136
BseNI ACTGG 5 cut(s) 424, 612, 867, 1046, 1346
BseRI GAGGAG 1 cut(s) 676
Bsh1236I CGCG 2 cut(s) 912, 1270
Bsh1285I CGRYCG 1 cut(s) 788
BshFI GGCC 6 cut(s) 18, 147, 745, 1018, 1233, 1280
BshVI ATCGAT 1 cut(s) 966
BsiEI CGRYCG 1 cut(s) 788
BsiHKAI GWGCWC 1 cut(s) 784
BsiSI CCGG 3 cut(s) 329, 704, 1238
BslFI GGGAC 2 cut(s) 528, 624
BslI CCNNNNNNNGG 1 cut(s) 411
BsmAI GTCTC 6 cut(s) 261, 381, 481, 766, 913, 991
BsmFI GGGAC 2 cut(s) 528, 624
BsnI GGCC 6 cut(s) 18, 147, 745, 1018, 1233, 1280
Bso31I GGTCTC 1 cut(s) 481
Bsp1286I GDGCHC 2 cut(s) 318, 784
Bsp13I TCCGGA 1 cut(s) 328
Bsp1407I TGTACA 1 cut(s) 195
Bsp143I GATC 7 cut(s) 24, 78, 363, 414, 785, 879, 1024
Bsp19I CCATGG 1 cut(s) 166
Bsp68I TCGCGA 1 cut(s) 1270
BspANI GGCC 6 cut(s) 18, 147, 745, 1018, 1233, 1280
BspCNI CTCAG 3 cut(s) 491, 532, 1135
BspDI ATCGAT 1 cut(s) 966
BspEI TCCGGA 1 cut(s) 328
BspFNI CGCG 2 cut(s) 912, 1270
BspHI TCATGA 1 cut(s) 1008
BspLI GGNNCC 2 cut(s) 516, 612
BspPI GGATC 3 cut(s) 358, 422, 1032
BspTI CTTAAG 2 cut(s) 220, 292
BspTNI GGTCTC 1 cut(s) 481
BsrGI TGTACA 1 cut(s) 195
BsrI ACTGG 5 cut(s) 424, 612, 867, 1046, 1346
BssECI CCNNGG 5 cut(s) 166, 410, 702, 1020, 1294
BssMI GATC 7 cut(s) 24, 78, 363, 414, 785, 879, 1024
BssT1I CCWWGG 2 cut(s) 166, 1020
Bst2UI CCWGG 1 cut(s) 208
BstAFI CTTAAG 2 cut(s) 220, 292
BstAUI TGTACA 1 cut(s) 195
BstC8I GCNNGC 2 cut(s) 1016, 1278
BstDEI CTNAG 5 cut(s) 380, 478, 540, 654, 1122
BstDSI CCRYGG 3 cut(s) 166, 410, 1294
BstF5I GGATG 1 cut(s) 337
BstFNI CGCG 2 cut(s) 912, 1270
BstKTI GATC 7 cut(s) 27, 81, 366, 417, 788, 882, 1027
BstMAI GTCTC 6 cut(s) 261, 381, 481, 766, 913, 991
BstMBI GATC 7 cut(s) 24, 78, 363, 414, 785, 879, 1024
BstMCI CGRYCG 1 cut(s) 788
BstMWI GCNNNNNNNGC 2 cut(s) 659, 1286
BstNI CCWGG 1 cut(s) 208
BstNSI RCATGY 1 cut(s) 196
BstSCI CCNGG 2 cut(s) 206, 702
BstUI CGCG 2 cut(s) 912, 1270
BstV2I GAAGAC 1 cut(s) 868
BstX2I RGATCY 3 cut(s) 78, 879, 1024
BstYI RGATCY 3 cut(s) 78, 879, 1024
Bsu15I ATCGAT 1 cut(s) 966
BsuRI GGCC 6 cut(s) 18, 147, 745, 1018, 1233, 1280
BsuTUI ATCGAT 1 cut(s) 966
BtgI CCRYGG 3 cut(s) 166, 410, 1294
BtsCI GGATG 1 cut(s) 337
BtsIMutI CAGTG 3 cut(s) 417, 645, 1109
BtuMI TCGCGA 1 cut(s) 1270
Cac8I GCNNGC 2 cut(s) 1016, 1278
CaiI CAGNNNCTG 1 cut(s) 922
CciI TCATGA 1 cut(s) 1008
Cfr13I GGNCC 6 cut(s) 146, 515, 611, 947, 1017, 1074
ClaI ATCGAT 1 cut(s) 966
Csp6I GTAC 3 cut(s) 196, 914, 1113
CviQI GTAC 3 cut(s) 196, 914, 1113
DdeI CTNAG 5 cut(s) 380, 478, 540, 654, 1122
DpnI GATC 7 cut(s) 26, 80, 365, 416, 787, 881, 1026
DpnII GATC 7 cut(s) 24, 78, 363, 414, 785, 879, 1024
EaeI YGGCCR 2 cut(s) 743, 1278
Ecl136II GAGCTC 1 cut(s) 782
Eco130I CCWWGG 2 cut(s) 166, 1020
Eco147I AGGCCT 1 cut(s) 18
Eco24I GRGCYC 2 cut(s) 318, 784
Eco31I GGTCTC 1 cut(s) 481
Eco47I GGWCC 4 cut(s) 515, 611, 947, 1074
Eco53kI GAGCTC 1 cut(s) 782
Eco57I CTGAAG 2 cut(s) 669, 1085
EcoICRI GAGCTC 1 cut(s) 782
EcoO109I RGGNCCY 4 cut(s) 146, 611, 947, 1017
EcoRII CCWGG 1 cut(s) 206
EcoT14I CCWWGG 2 cut(s) 166, 1020
EcoT22I ATGCAT 1 cut(s) 1072
EcoT38I GRGCYC 2 cut(s) 318, 784
ErhI CCWWGG 2 cut(s) 166, 1020
FalI AAGNNNNNCTT 2 cut(s) 647, 679
FaqI GGGAC 2 cut(s) 528, 624
FbaI TGATCA 1 cut(s) 24
FokI GGATG 1 cut(s) 344
FriOI GRGCYC 2 cut(s) 318, 784
FspBI CTAG 2 cut(s) 437, 485
GsuI CTGGAG 1 cut(s) 573
HaeIII GGCC 6 cut(s) 18, 147, 745, 1018, 1233, 1280
HapII CCGG 3 cut(s) 329, 704, 1238
HindIII AAGCTT 1 cut(s) 843
HinfI GANTC 8 cut(s) 100, 138, 374, 546, 673, 901, 907, 939
HpaII CCGG 3 cut(s) 329, 704, 1238
HphI GGTGA 5 cut(s) 485, 850, 1144, 1262, 1273
Hpy166II GTNNAC 3 cut(s) 99, 265, 900
Hpy188I TCNGA 7 cut(s) 105, 143, 771, 906, 944, 1188, 1202
Hpy188III TCNNGA 5 cut(s) 12, 329, 552, 1009, 1269
Hpy8I GTNNAC 3 cut(s) 99, 265, 900
HpyAV CCTTC 7 cut(s) 417, 700, 1210, 1241, 1244, 1266, 1300
HpyCH4IV ACGT 1 cut(s) 1311
HpyCH4V TGCA 7 cut(s) 111, 526, 641, 1070, 1152, 1211, 1323
HpyF10VI GCNNNNNNNGC 2 cut(s) 659, 1286
HpyF3I CTNAG 5 cut(s) 380, 478, 540, 654, 1122
HpySE526I ACGT 1 cut(s) 1311
Kpn2I TCCGGA 1 cut(s) 328
Ksp22I TGATCA 1 cut(s) 24
Kzo9I GATC 7 cut(s) 24, 78, 363, 414, 785, 879, 1024
LmnI GCTCC 4 cut(s) 210, 277, 689, 779
LweI GCATC 1 cut(s) 535
MaeI CTAG 2 cut(s) 437, 485
MaeII ACGT 1 cut(s) 1311
MaeIII GTNAC 2 cut(s) 1029, 1132
MalI GATC 7 cut(s) 26, 80, 365, 416, 787, 881, 1026
MboI GATC 7 cut(s) 24, 78, 363, 414, 785, 879, 1024
MboII GAAGA 3 cut(s) 873, 1091, 1102
MflI RGATCY 3 cut(s) 78, 879, 1024
MhlI GDGCHC 2 cut(s) 318, 784
MlsI TGGCCA 1 cut(s) 1280
MluCI AATT 4 cut(s) 106, 506, 853, 1093
MluNI TGGCCA 1 cut(s) 1280
MlyI GAGTC 3 cut(s) 94, 383, 895
MmeI TCCRAC 3 cut(s) 213, 749, 1014
Mox20I TGGCCA 1 cut(s) 1280
Mph1103I ATGCAT 1 cut(s) 1072
MroI TCCGGA 1 cut(s) 328
MscI TGGCCA 1 cut(s) 1280
MseI TTAA 5 cut(s) 75, 221, 293, 833, 1307
MslI CAYNNNNRTG 1 cut(s) 1065
Msp20I TGGCCA 1 cut(s) 1280
MspCI CTTAAG 2 cut(s) 220, 292
MspI CCGG 3 cut(s) 329, 704, 1238
MspR9I CCNGG 2 cut(s) 208, 704
MvaI CCWGG 1 cut(s) 208
MvnI CGCG 2 cut(s) 912, 1270
MwoI GCNNNNNNNGC 2 cut(s) 659, 1286
NciI CCSGG 1 cut(s) 704
NcoI CCATGG 1 cut(s) 166
NdeII GATC 7 cut(s) 24, 78, 363, 414, 785, 879, 1024
NlaIV GGNNCC 2 cut(s) 516, 612
NmuCI GTSAC 2 cut(s) 1029, 1132
NruI TCGCGA 1 cut(s) 1270
NsiI ATGCAT 1 cut(s) 1072
NspI RCATGY 1 cut(s) 196
PagI TCATGA 1 cut(s) 1008
PceI AGGCCT 1 cut(s) 18
PciI ACATGT 1 cut(s) 192
PfeI GAWTC 5 cut(s) 138, 546, 673, 907, 939
Ple19I CGATCG 1 cut(s) 788
PleI GAGTC 3 cut(s) 94, 382, 895
PpsI GAGTC 3 cut(s) 94, 382, 895
PpuMI RGGWCCY 2 cut(s) 611, 947
PscI ACATGT 1 cut(s) 192
PsiI TTATAA 1 cut(s) 1351
Psp124BI GAGCTC 1 cut(s) 784
Psp5II RGGWCCY 2 cut(s) 611, 947
Psp6I CCWGG 1 cut(s) 206
PspGI CCWGG 1 cut(s) 206
PspN4I GGNNCC 2 cut(s) 516, 612
PspPI GGNCC 6 cut(s) 146, 515, 611, 947, 1017, 1074
PspPPI RGGWCCY 2 cut(s) 611, 947
PstNI CAGNNNCTG 1 cut(s) 922
PsuI RGATCY 3 cut(s) 78, 879, 1024
PvuI CGATCG 1 cut(s) 788
RruI TCGCGA 1 cut(s) 1270
RsaI GTAC 3 cut(s) 197, 915, 1114
RsaNI GTAC 3 cut(s) 196, 914, 1113
RseI CAYNNNNRTG 1 cut(s) 1065
SacI GAGCTC 1 cut(s) 784
SaqAI TTAA 5 cut(s) 75, 221, 293, 833, 1307
Sau3AI GATC 7 cut(s) 24, 78, 363, 414, 785, 879, 1024
Sau96I GGNCC 6 cut(s) 146, 515, 611, 947, 1017, 1074
SchI GAGTC 3 cut(s) 94, 383, 895
ScrFI CCNGG 2 cut(s) 208, 704
SduI GDGCHC 2 cut(s) 318, 784
SfaNI GCATC 1 cut(s) 535
SinI GGWCC 4 cut(s) 515, 611, 947, 1074
SmiMI CAYNNNNRTG 1 cut(s) 1065
SmlI CTYRAG 2 cut(s) 220, 292
SmoI CTYRAG 2 cut(s) 220, 292
Sse9I AATT 4 cut(s) 106, 506, 853, 1093
SseBI AGGCCT 1 cut(s) 18
SspMI CTAG 2 cut(s) 437, 485
SstI GAGCTC 1 cut(s) 784
StuI AGGCCT 1 cut(s) 18
StyD4I CCNGG 2 cut(s) 206, 702
StyI CCWWGG 2 cut(s) 166, 1020
TaiI ACGT 1 cut(s) 1314
TaqI TCGA 6 cut(s) 312, 616, 754, 784, 812, 966
TasI AATT 4 cut(s) 106, 506, 853, 1093
TatI WGTACW 1 cut(s) 195
TfiI GAWTC 5 cut(s) 138, 546, 673, 907, 939
Tru1I TTAA 5 cut(s) 75, 221, 293, 833, 1307
Tru9I TTAA 5 cut(s) 75, 221, 293, 833, 1307
TscAI CASTG 3 cut(s) 424, 652, 1109
TseFI GTSAC 2 cut(s) 1029, 1132
Tsp45I GTSAC 2 cut(s) 1029, 1132
TspDTI ATGAA 4 cut(s) 336, 513, 647, 1055
TspGWI ACGGA 3 cut(s) 427, 1151, 1283
TspRI CASTG 3 cut(s) 424, 652, 1109
Vha464I CTTAAG 2 cut(s) 220, 292
VpaK11BI GGWCC 4 cut(s) 515, 611, 947, 1074
XceI RCATGY 1 cut(s) 196
XcmI CCANNNNNNNNNTGG 1 cut(s) 1292
XspI CTAG 2 cut(s) 437, 485
Zsp2I ATGCAT 1 cut(s) 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.