RchiOBHm_Chr4g0446641

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
N/A
Physical Location & Seq
Reverse (-)
66762905 .. 66763192
288 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 288 bp
ATGAGCTATATGGTTGGATATGGATCGAATTATCCGAAGCAAATTCATCACAGAGGAGCATCAATCGTCTCAATCAAGAAGGATCCAGCTCCTGTAACATGCCAAGGTGGGTTTGACTGGTTTCACAGACCCGAGCCCAACCCGAACGTGCTGGAGGGAGCAATTGTGACCCCAGATGAAAATGATGGTTTTGCGGACGACAGGAACAACTTTCAGTTAGCTGAACCAACGACCGTTACTAATGCTCCACTAGTTGGTGTCTTAGCTCACCTTGCTCAACTCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

95

Amino Acids

10.36

Weight (kDa)

5.34

Isoelectric Point (pI)

24.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 1 - 88 9.7e-23 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 254
AciI CCGC 1 cut(s) 194
AclWI GGATC 3 cut(s) 31, 77, 90
AcsI RAATTY 1 cut(s) 42
AfiI CCNNNNNNNGG 1 cut(s) 254
AhlI ACTAGT 1 cut(s) 250
AluBI AGCT 4 cut(s) 6, 89, 221, 266
AluI AGCT 4 cut(s) 6, 89, 221, 266
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 3 cut(s) 31, 77, 90
AlwNI CAGNNNCTG 1 cut(s) 92
Ama87I CYCGRG 1 cut(s) 131
ApoI RAATTY 1 cut(s) 42
AsuHPI GGTGA 1 cut(s) 260
AvaI CYCGRG 1 cut(s) 131
BamHI GGATCC 1 cut(s) 82
BanII GRGCYC 1 cut(s) 138
BccI CCATC 1 cut(s) 179
BcoDI GTCTC 1 cut(s) 73
BcuI ACTAGT 1 cut(s) 250
BfaI CTAG 1 cut(s) 251
BmeT110I CYCGRG 1 cut(s) 131
BmiI GGNNCC 1 cut(s) 84
BmsI GCATC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 173
BsaBI GATNNNNATC 1 cut(s) 22
BsaJI CCNNGG 1 cut(s) 103
BsaXI ACNNNNNCTCC 4 cut(s) 150, 180, 229, 259
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse1I ACTGG 1 cut(s) 122
Bse8I GATNNNNATC 1 cut(s) 22
BseDI CCNNGG 1 cut(s) 103
BseJI GATNNNNATC 1 cut(s) 22
BseLI CCNNNNNNNGG 1 cut(s) 254
BseNI ACTGG 1 cut(s) 122
BseRI GAGGAG 1 cut(s) 69
Bsh1285I CGRYCG 1 cut(s) 234
BsiEI CGRYCG 1 cut(s) 234
BsiHKCI CYCGRG 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 254
BsmAI GTCTC 1 cut(s) 73
BsmBI CGTCTC 1 cut(s) 73
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 138
Bsp143I GATC 2 cut(s) 23, 82
BspACI CCGC 1 cut(s) 194
BspLI GGNNCC 1 cut(s) 84
BspPI GGATC 3 cut(s) 31, 77, 90
BsrI ACTGG 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 103
BssMI GATC 2 cut(s) 23, 82
BssT1I CCWWGG 1 cut(s) 103
Bst4CI ACNGT 1 cut(s) 235
BstDEI CTNAG 1 cut(s) 262
BstKTI GATC 2 cut(s) 26, 85
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 2 cut(s) 23, 82
BstMCI CGRYCG 1 cut(s) 234
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
CaiI CAGNNNCTG 1 cut(s) 92
CviAII CATG 1 cut(s) 99
CviJI RGCY 5 cut(s) 6, 89, 136, 221, 266
CviKI_1 RGCY 5 cut(s) 6, 89, 136, 221, 266
DdeI CTNAG 1 cut(s) 262
DpnI GATC 2 cut(s) 25, 84
DpnII GATC 2 cut(s) 23, 82
Eco130I CCWWGG 1 cut(s) 103
Eco24I GRGCYC 1 cut(s) 138
Eco88I CYCGRG 1 cut(s) 131
EcoT14I CCWWGG 1 cut(s) 103
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 103
Esp3I CGTCTC 1 cut(s) 73
FaeI CATG 1 cut(s) 102
FaiI YATR 5 cut(s) 9, 11, 21, 100, 286
FatI CATG 1 cut(s) 98
FriOI GRGCYC 1 cut(s) 138
FspBI CTAG 1 cut(s) 251
GsuI CTGGAG 1 cut(s) 173
Hin1II CATG 1 cut(s) 102
HphI GGTGA 1 cut(s) 260
Hpy188I TCNGA 1 cut(s) 36
Hpy188III TCNNGA 1 cut(s) 76
HpyAV CCTTC 1 cut(s) 73
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4IV ACGT 1 cut(s) 147
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpyF3I CTNAG 1 cut(s) 262
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 1 cut(s) 102
Kzo9I GATC 2 cut(s) 23, 82
LmnI GCTCC 4 cut(s) 56, 94, 158, 250
LpnPI CCDG 6 cut(s) 99, 103, 105, 137, 186, 187
LweI GCATC 1 cut(s) 68
MaeI CTAG 1 cut(s) 251
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 3 cut(s) 94, 166, 235
MalI GATC 2 cut(s) 25, 84
MboI GATC 2 cut(s) 23, 82
MfeI CAATTG 1 cut(s) 162
MflI RGATCY 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 138
MluCI AATT 3 cut(s) 28, 42, 162
MnlI CCTC 2 cut(s) 47, 148
MunI CAATTG 1 cut(s) 162
MwoI GCNNNNNNNGC 1 cut(s) 272
NdeII GATC 2 cut(s) 23, 82
NlaIII CATG 1 cut(s) 102
NlaIV GGNNCC 1 cut(s) 84
NmuCI GTSAC 1 cut(s) 166
NspI RCATGY 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 32
PflMI CCANNNNNTGG 1 cut(s) 254
PspN4I GGNNCC 1 cut(s) 84
PstNI CAGNNNCTG 1 cut(s) 92
PsuI RGATCY 1 cut(s) 82
Sau3AI GATC 2 cut(s) 23, 82
SduI GDGCHC 1 cut(s) 138
SetI ASST 7 cut(s) 8, 91, 109, 150, 223, 268, 273
SfaNI GCATC 1 cut(s) 68
SpeI ACTAGT 1 cut(s) 250
Sse9I AATT 3 cut(s) 28, 42, 162
SsiI CCGC 1 cut(s) 194
SspMI CTAG 1 cut(s) 251
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 1 cut(s) 235
TaiI ACGT 1 cut(s) 150
TaqI TCGA 1 cut(s) 26
TasI AATT 3 cut(s) 28, 42, 162
TseFI GTSAC 1 cut(s) 166
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 2 cut(s) 35, 192
Van91I CCANNNNNTGG 1 cut(s) 254
XapI RAATTY 1 cut(s) 42
XceI RCATGY 1 cut(s) 102
XspI CTAG 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.