RchiOBHm_Chr3g0465451

Tropinone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
N/A
Physical Location & Seq
Forward (+)
12208413 .. 12210210
1798 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 453 bp
ATGACCGCCCTCGTCACTGGTGGAACCAAGGGCATAGGGGTTCGAGAATGGAAGAACAAGGGTTTCAAAGTGACTGTTTCAGTTTGCGACCTCACCTCTAAATCTGAAAGAGAGGAGCTCATTAAGGAGGTCTCGTCTGTTTTTGATGGCAAGCTTAACATTCTTTCACACTCAGGAGTACTACAGATTATAGTCTTCGAAGACTTGTCAAGCATGATGAGTACCAACGTAGAATCTCCTTACCATCTTTGCCAACTTGCATATCCTCTCTTGAAGGCCTCTGGAAATGGAAGTATTGTGTTCGTCGCCTCTATCGCCGGTGTTATATCTCTACCTAGACTATCCGCCTATGCAGCAACGAAGACTGGTGGTCTTCAATGCCTTCCTTTCATTGTCTGTGGTTCCTCTTTCAATCAATGCACACAATATAAGCTCCTTTCCTTGCAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

150

Amino Acids

16.04

Weight (kDa)

8.62

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 15 - 124 4.4e-17 short chain dehydrogenase
adh_short_C2 PF13561 22 - 123 3.5e-11 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 6, 345
AfaI GTAC 2 cut(s) 180, 223
AgsI TTSAA 4 cut(s) 67, 274, 377, 412
AhdI GACNNNNNGTC 1 cut(s) 369
AluBI AGCT 3 cut(s) 118, 154, 433
AluI AGCT 3 cut(s) 118, 154, 433
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 1 cut(s) 136
AoxI GGCC 1 cut(s) 276
ApeKI GCWGC 1 cut(s) 353
AsuHPI GGTGA 1 cut(s) 85
AsuII TTCGAA 1 cut(s) 198
BanII GRGCYC 1 cut(s) 120
BbsI GAAGAC 4 cut(s) 187, 207, 365, 368
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 1 cut(s) 365
BccI CCATC 2 cut(s) 140, 252
BcoDI GTCTC 1 cut(s) 136
BfaI CTAG 1 cut(s) 336
BfmI CTRYAG 1 cut(s) 182
BisI GCNGC 1 cut(s) 354
BlsI GCNGC 1 cut(s) 355
BmcAI AGTACT 1 cut(s) 180
BmeRI GACNNNNNGTC 1 cut(s) 369
BmiI GGNNCC 2 cut(s) 25, 403
BpiI GAAGAC 4 cut(s) 187, 207, 365, 368
BplI GAGNNNNNCTC 2 cut(s) 102, 134
Bpu14I TTCGAA 1 cut(s) 198
BsaI GGTCTC 1 cut(s) 136
BsaJI CCNNGG 1 cut(s) 27
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 2 cut(s) 22, 370
BseDI CCNNGG 1 cut(s) 27
BseMII CTCAG 1 cut(s) 186
BseNI ACTGG 2 cut(s) 22, 370
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 1 cut(s) 365
BshFI GGCC 1 cut(s) 278
BsiHKAI GWGCWC 1 cut(s) 120
BsiSI CCGG 1 cut(s) 318
BsmAI GTCTC 1 cut(s) 136
BsnI GGCC 1 cut(s) 278
Bso31I GGTCTC 1 cut(s) 136
Bsp119I TTCGAA 1 cut(s) 198
Bsp1286I GDGCHC 1 cut(s) 120
BspACI CCGC 2 cut(s) 6, 345
BspANI GGCC 1 cut(s) 278
BspCNI CTCAG 1 cut(s) 185
BspLI GGNNCC 2 cut(s) 25, 403
BspT104I TTCGAA 1 cut(s) 198
BspTNI GGTCTC 1 cut(s) 136
BsrFI RCCGGY 1 cut(s) 317
BsrI ACTGG 2 cut(s) 22, 370
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 1 cut(s) 27
BssT1I CCWWGG 1 cut(s) 27
Bst4CI ACNGT 1 cut(s) 76
BstBI TTCGAA 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 152, 447
BstDEI CTNAG 1 cut(s) 172
BstMAI GTCTC 1 cut(s) 136
BstMWI GCNNNNNNNGC 2 cut(s) 314, 353
BstSFI CTRYAG 1 cut(s) 182
BstV1I GCAGC 1 cut(s) 365
BstV2I GAAGAC 4 cut(s) 187, 207, 365, 368
BsuRI GGCC 1 cut(s) 278
BtsIMutI CAGTG 1 cut(s) 15
Cac8I GCNNGC 2 cut(s) 152, 447
Cfr10I RCCGGY 1 cut(s) 317
Csp6I GTAC 2 cut(s) 179, 222
CviAII CATG 1 cut(s) 214
CviJI RGCY 4 cut(s) 118, 154, 278, 433
CviKI_1 RGCY 4 cut(s) 118, 154, 278, 433
CviQI GTAC 2 cut(s) 179, 222
DdeI CTNAG 1 cut(s) 172
DriI GACNNNNNGTC 1 cut(s) 369
Eam1105I GACNNNNNGTC 1 cut(s) 369
EciI GGCGGA 1 cut(s) 334
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 27
Eco147I AGGCCT 1 cut(s) 278
Eco24I GRGCYC 1 cut(s) 120
Eco31I GGTCTC 1 cut(s) 136
Eco53kI GAGCTC 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 118
EcoT14I CCWWGG 1 cut(s) 27
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 1 cut(s) 217
FaiI YATR 8 cut(s) 35, 191, 215, 262, 326, 351, 429, 451
FatI CATG 1 cut(s) 213
Fnu4HI GCNGC 1 cut(s) 354
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 1 cut(s) 354
FspBI CTAG 1 cut(s) 336
GluI GCNGC 1 cut(s) 354
HaeIII GGCC 1 cut(s) 278
HapII CCGG 1 cut(s) 318
Hin1II CATG 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 1 cut(s) 233
HpaII CCGG 1 cut(s) 318
HphI GGTGA 1 cut(s) 85
Hpy188I TCNGA 1 cut(s) 106
Hpy188III TCNNGA 4 cut(s) 44, 174, 271, 282
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 2 cut(s) 268, 392
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 4 cut(s) 260, 353, 420, 445
HpyF10VI GCNNNNNNNGC 2 cut(s) 314, 353
HpyF3I CTNAG 1 cut(s) 172
HpySE526I ACGT 1 cut(s) 228
Hsp92II CATG 1 cut(s) 217
LmnI GCTCC 2 cut(s) 115, 438
LpnPI CCDG 5 cut(s) 3, 159, 267, 331, 351
Lsp1109I GCAGC 1 cut(s) 365
MaeI CTAG 1 cut(s) 336
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 2 cut(s) 13, 70
MboII GAAGA 5 cut(s) 64, 187, 212, 365, 373
MhlI GDGCHC 1 cut(s) 120
MnlI CCTC 9 cut(s) 20, 101, 106, 106, 121, 276, 289, 319, 415
MseI TTAA 2 cut(s) 123, 156
MspI CCGG 1 cut(s) 318
MwoI GCNNNNNNNGC 2 cut(s) 314, 353
NlaIII CATG 1 cut(s) 217
NlaIV GGNNCC 2 cut(s) 25, 403
NmuCI GTSAC 2 cut(s) 13, 70
NspV TTCGAA 1 cut(s) 198
PceI AGGCCT 1 cut(s) 278
PfeI GAWTC 1 cut(s) 233
PkrI GCNGC 1 cut(s) 355
Psp124BI GAGCTC 1 cut(s) 120
PspN4I GGNNCC 2 cut(s) 25, 403
RsaI GTAC 2 cut(s) 180, 223
RsaNI GTAC 2 cut(s) 179, 222
SacI GAGCTC 1 cut(s) 120
SaqAI TTAA 2 cut(s) 123, 156
SatI GCNGC 1 cut(s) 354
ScaI AGTACT 1 cut(s) 180
SduI GDGCHC 1 cut(s) 120
SetI ASST 8 cut(s) 93, 98, 120, 132, 156, 231, 337, 435
SfcI CTRYAG 1 cut(s) 182
SfuI TTCGAA 1 cut(s) 198
SgrAI CRCCGGYG 1 cut(s) 317
SseBI AGGCCT 1 cut(s) 278
SsiI CCGC 2 cut(s) 6, 345
SspMI CTAG 1 cut(s) 336
SstI GAGCTC 1 cut(s) 120
StuI AGGCCT 1 cut(s) 278
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 1 cut(s) 231
TaqI TCGA 2 cut(s) 43, 198
TatI WGTACW 1 cut(s) 178
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 2 cut(s) 123, 156
Tru9I TTAA 2 cut(s) 123, 156
TscAI CASTG 1 cut(s) 22
TseFI GTSAC 2 cut(s) 13, 70
TseI GCWGC 1 cut(s) 353
Tsp45I GTSAC 2 cut(s) 13, 70
TspDTI ATGAA 1 cut(s) 379
TspRI CASTG 1 cut(s) 22
XspI CTAG 1 cut(s) 336
ZrmI AGTACT 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.