RchiOBHm_Chr3g0483881

DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
N/A
Physical Location & Seq
Forward (+)
30211559 .. 30214366
2808 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 957 bp
ATGCAAAGCTTCGACCCGTCAAAAATGCTCACTGATTCGGGAAAACTTCAGACACTTGATATATTGTTGAAACGCTTGAGGGCAGAAAATCATCGTGTTCTCTTGTTTGCACAGATGACAAAGATGCTGAATATTCTTGAGGACTACATGAACTACAGGAAATATAGATACCTCAGACTCGATGGATCTTCCACCATTATGGATCGTAGAGACATGGTCAGAGACTTCCAGCAACGAAGTGATATTTTTGTTTTCTTGCTGAGTACAAGAGCTGGTGGACTAGGCATTAACTTGACAGCTGCAGATACTGTCATATTTTATGAAAGTGATTGGAATCCGACCCTGGATCTACAGGCAATGGATAGAGCTCATCGGTTGGGCCAGACAAAAGATGTTACTGTCTACCGCCTAATTTGTAAAGAAACGGTTGAGGAGAAGATTCTGCAAAGAGCAAGTCAGAAAAATACTGTGCAGCAGCTTGTAATGATGGGTGGTCATGTTCAAGGTGACCTTTTGGCTCCCGAGGATGTTGTATCTCTACTTCTCGATGATGTCCAGTTGGAGCAGAAATTAAGAGAAGCTCCATCACAGGTTAAGGATAGACAAAAGAAGAAACAGACAAAGGGTATACGGGTAGATGCAGAAGGTGATGCCTCTTTGGAAGATTTAACAAACACTGCTGCATCACAAGGTACTGGAAATGAGGAATCTCCGGATGTCGAAAGATCAAAATCCAATAATAAAAAGAGGAAAACAGTGCCTGACAAGCATACTCCGCGGCCGAAGAATCCTCAAAGTATGGATGAACCAGAGGGCTATGAATTGGAGGATTCCTTACAAAATACTGATCCACAAGGTACAAGACCCAAGAGGCCAAAGAGGTCAAAGAAGAGTGTAAATGAAACTCTTGAACCTGCATTTACAGCGGCATCCCCTGTGGTCCCGAGGCAGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBINO Helicase_C SNF2_N

Protein Analysis

318

Amino Acids

36.19

Weight (kDa)

8.74

Isoelectric Point (pI)

52.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 16 - 127 2.3e-24 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 922
AccI GTMKAC 2 cut(s) 402, 628
AccII CGCG 1 cut(s) 778
AccIII TCCGGA 1 cut(s) 712
AciI CCGC 4 cut(s) 406, 776, 778, 926
AclWI GGATC 4 cut(s) 193, 210, 354, 842
AcoI YGGCCR 1 cut(s) 779
AcuI CTGAAG 1 cut(s) 32
AfaI GTAC 3 cut(s) 265, 694, 859
AgsI TTSAA 3 cut(s) 70, 503, 911
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 6 cut(s) 9, 272, 299, 368, 478, 581
AluI AGCT 6 cut(s) 9, 272, 299, 368, 478, 581
Alw21I GWGCWC 1 cut(s) 370
Alw26I GTCTC 2 cut(s) 204, 216
AlwI GGATC 4 cut(s) 193, 210, 354, 842
AlwNI CAGNNNCTG 2 cut(s) 308, 761
Ama87I CYCGRG 2 cut(s) 521, 943
Aor13HI TCCGGA 1 cut(s) 712
AoxI GGCC 3 cut(s) 379, 779, 872
ApeKI GCWGC 4 cut(s) 299, 472, 475, 680
ArsI GACNNNNNNTTYG 2 cut(s) 439, 471
AspS9I GGNCC 2 cut(s) 379, 940
AsuHPI GGTGA 2 cut(s) 518, 659
AvaI CYCGRG 2 cut(s) 521, 943
AvaII GGWCC 1 cut(s) 940
BanII GRGCYC 1 cut(s) 370
BarI GAAGNNNNNNTAC 2 cut(s) 525, 557
Bbv12I GWGCWC 1 cut(s) 370
BbvI GCAGC 4 cut(s) 286, 484, 487, 667
BccI CCATC 3 cut(s) 176, 481, 592
BciT130I CCWGG 1 cut(s) 344
BcoDI GTCTC 2 cut(s) 204, 216
BfaI CTAG 1 cut(s) 281
BfmI CTRYAG 3 cut(s) 154, 300, 350
BfuAI ACCTGC 1 cut(s) 922
BisI GCNGC 6 cut(s) 300, 473, 476, 681, 779, 927
BlsI GCNGC 6 cut(s) 301, 474, 477, 682, 780, 928
Bme1390I CCNGG 1 cut(s) 344
Bme18I GGWCC 1 cut(s) 940
BmeT110I CYCGRG 2 cut(s) 521, 943
BmgT120I GGNCC 2 cut(s) 379, 940
BmiI GGNNCC 2 cut(s) 519, 942
BmrFI CCNGG 1 cut(s) 344
BmsI GCATC 5 cut(s) 114, 628, 640, 692, 938
BpuEI CTTGAG 2 cut(s) 97, 158
BsaBI GATNNNNATC 2 cut(s) 333, 730
BsaJI CCNNGG 4 cut(s) 342, 522, 776, 944
BsaWI WCCGGW 1 cut(s) 712
Bse1I ACTGG 2 cut(s) 556, 700
Bse3DI GCAATG 1 cut(s) 363
Bse8I GATNNNNATC 2 cut(s) 333, 730
BseAI TCCGGA 1 cut(s) 712
BseBI CCWGG 1 cut(s) 344
BseDI CCNNGG 4 cut(s) 342, 522, 776, 944
BseGI GGATG 4 cut(s) 532, 721, 808, 929
BseJI GATNNNNATC 2 cut(s) 333, 730
BseMI GCAATG 1 cut(s) 363
BseMII CTCAG 2 cut(s) 187, 251
BseNI ACTGG 2 cut(s) 556, 700
BseRI GAGGAG 1 cut(s) 446
BseX3I CGGCCG 1 cut(s) 779
BseXI GCAGC 4 cut(s) 286, 484, 487, 667
BsgI GTGCAG 1 cut(s) 491
Bsh1236I CGCG 1 cut(s) 778
Bsh1285I CGRYCG 1 cut(s) 782
BshFI GGCC 3 cut(s) 381, 781, 874
BsiEI CGRYCG 1 cut(s) 782
BsiHKAI GWGCWC 1 cut(s) 370
BsiHKCI CYCGRG 2 cut(s) 521, 943
BsiSI CCGG 1 cut(s) 713
BslFI GGGAC 1 cut(s) 926
BsmAI GTCTC 2 cut(s) 204, 216
BsmFI GGGAC 1 cut(s) 926
BsnI GGCC 3 cut(s) 381, 781, 874
BsoBI CYCGRG 2 cut(s) 521, 943
Bsp1286I GDGCHC 1 cut(s) 370
Bsp13I TCCGGA 1 cut(s) 712
Bsp143I GATC 5 cut(s) 185, 202, 346, 725, 847
BspACI CCGC 4 cut(s) 406, 776, 778, 926
BspANI GGCC 3 cut(s) 381, 781, 874
BspCNI CTCAG 2 cut(s) 186, 252
BspEI TCCGGA 1 cut(s) 712
BspFNI CGCG 1 cut(s) 778
BspLI GGNNCC 2 cut(s) 519, 942
BspMAI CTGCAG 1 cut(s) 304
BspMI ACCTGC 1 cut(s) 922
BspPI GGATC 4 cut(s) 193, 210, 354, 842
BsrDI GCAATG 1 cut(s) 363
BsrI ACTGG 2 cut(s) 556, 700
BssECI CCNNGG 4 cut(s) 342, 522, 776, 944
BssMI GATC 5 cut(s) 185, 202, 346, 725, 847
BssNAI GTATAC 1 cut(s) 629
Bst1107I GTATAC 1 cut(s) 629
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 5 cut(s) 310, 400, 427, 469, 757
Bst6I CTCTTC 1 cut(s) 884
BstDEI CTNAG 2 cut(s) 173, 260
BstDSI CCRYGG 1 cut(s) 776
BstEII GGTNACC 1 cut(s) 506
BstF5I GGATG 4 cut(s) 532, 721, 808, 929
BstFNI CGCG 1 cut(s) 778
BstKTI GATC 5 cut(s) 188, 205, 349, 728, 850
BstMAI GTCTC 2 cut(s) 204, 216
BstMBI GATC 5 cut(s) 185, 202, 346, 725, 847
BstMCI CGRYCG 1 cut(s) 782
BstMWI GCNNNNNNNGC 3 cut(s) 766, 775, 923
BstNI CCWGG 1 cut(s) 344
BstPI GGTNACC 1 cut(s) 506
BstSCI CCNGG 1 cut(s) 342
BstSFI CTRYAG 3 cut(s) 154, 300, 350
BstUI CGCG 1 cut(s) 778
BstV1I GCAGC 4 cut(s) 286, 484, 487, 667
BstX2I RGATCY 2 cut(s) 185, 346
BstXI CCANNNNNNTGG 1 cut(s) 199
BstYI RGATCY 2 cut(s) 185, 346
BstZ17I GTATAC 1 cut(s) 629
BstZI CGGCCG 1 cut(s) 779
BsuRI GGCC 3 cut(s) 381, 781, 874
BtgI CCRYGG 1 cut(s) 776
BtsCI GGATG 4 cut(s) 532, 721, 808, 929
BtsI GCAGTG 1 cut(s) 675
BtsIMutI CAGTG 3 cut(s) 30, 675, 762
BveI ACCTGC 1 cut(s) 922
CaiI CAGNNNCTG 2 cut(s) 308, 761
Cfr13I GGNCC 2 cut(s) 379, 940
Cfr42I CCGCGG 1 cut(s) 779
Csp6I GTAC 3 cut(s) 264, 693, 858
CviAII CATG 3 cut(s) 148, 214, 497
CviQI GTAC 3 cut(s) 264, 693, 858
DdeI CTNAG 2 cut(s) 173, 260
DpnI GATC 5 cut(s) 187, 204, 348, 727, 849
DpnII GATC 5 cut(s) 185, 202, 346, 725, 847
EaeI YGGCCR 1 cut(s) 779
EagI CGGCCG 1 cut(s) 779
Eam1104I CTCTTC 1 cut(s) 884
EarI CTCTTC 1 cut(s) 884
Ecl136II GAGCTC 1 cut(s) 368
EclXI CGGCCG 1 cut(s) 779
Eco24I GRGCYC 1 cut(s) 370
Eco47I GGWCC 1 cut(s) 940
Eco52I CGGCCG 1 cut(s) 779
Eco53kI GAGCTC 1 cut(s) 368
Eco57I CTGAAG 1 cut(s) 32
Eco88I CYCGRG 2 cut(s) 521, 943
Eco91I GGTNACC 1 cut(s) 506
EcoICRI GAGCTC 1 cut(s) 368
EcoO65I GGTNACC 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 342
EcoT38I GRGCYC 1 cut(s) 370
FaeI CATG 3 cut(s) 151, 217, 500
FalI AAGNNNNNCTT 2 cut(s) 495, 527
FaqI GGGAC 1 cut(s) 926
FatI CATG 3 cut(s) 147, 213, 496
FblI GTMKAC 2 cut(s) 402, 628
Fnu4HI GCNGC 6 cut(s) 300, 473, 476, 681, 779, 927
FokI GGATG 4 cut(s) 539, 728, 815, 916
FriOI GRGCYC 1 cut(s) 370
Fsp4HI GCNGC 6 cut(s) 300, 473, 476, 681, 779, 927
FspBI CTAG 1 cut(s) 281
GluI GCNGC 6 cut(s) 300, 473, 476, 681, 779, 927
HaeIII GGCC 3 cut(s) 381, 781, 874
HapII CCGG 1 cut(s) 713
Hin1II CATG 3 cut(s) 151, 217, 500
HindIII AAGCTT 1 cut(s) 7
HinfI GANTC 7 cut(s) 35, 177, 334, 439, 707, 787, 830
HpaII CCGG 1 cut(s) 713
HphI GGTGA 2 cut(s) 518, 659
Hpy166II GTNNAC 3 cut(s) 278, 403, 629
Hpy188I TCNGA 5 cut(s) 51, 176, 221, 339, 459
Hpy188III TCNNGA 7 cut(s) 39, 137, 521, 545, 713, 908, 943
Hpy8I GTNNAC 3 cut(s) 278, 403, 629
HpyAV CCTTC 1 cut(s) 638
HpyCH4III ACNGT 5 cut(s) 310, 400, 427, 469, 757
HpyCH4V TGCA 8 cut(s) 4, 110, 302, 445, 472, 641, 683, 917
HpyF10VI GCNNNNNNNGC 3 cut(s) 766, 775, 923
HpyF3I CTNAG 2 cut(s) 173, 260
Hsp92II CATG 3 cut(s) 151, 217, 500
Kpn2I TCCGGA 1 cut(s) 712
KspI CCGCGG 1 cut(s) 779
Kzo9I GATC 5 cut(s) 185, 202, 346, 725, 847
LmnI GCTCC 3 cut(s) 523, 562, 586
Lsp1109I GCAGC 4 cut(s) 286, 484, 487, 667
LweI GCATC 5 cut(s) 114, 628, 640, 692, 938
MaeI CTAG 1 cut(s) 281
MaeIII GTNAC 2 cut(s) 394, 506
MalI GATC 5 cut(s) 187, 204, 348, 727, 849
MboI GATC 5 cut(s) 185, 202, 346, 725, 847
MboII GAAGA 6 cut(s) 180, 448, 622, 674, 796, 901
MflI RGATCY 2 cut(s) 185, 346
MhlI GDGCHC 1 cut(s) 370
MluCI AATT 3 cut(s) 411, 569, 821
MlyI GAGTC 1 cut(s) 171
MmeI TCCRAC 2 cut(s) 362, 540
MroI TCCGGA 1 cut(s) 712
MseI TTAA 4 cut(s) 288, 572, 594, 668
MslI CAYNNNNRTG 1 cut(s) 197
MspA1I CMGCKG 3 cut(s) 299, 778, 926
MspI CCGG 1 cut(s) 713
MspR9I CCNGG 1 cut(s) 344
MvaI CCWGG 1 cut(s) 344
MvnI CGCG 1 cut(s) 778
MwoI GCNNNNNNNGC 3 cut(s) 766, 775, 923
NdeII GATC 5 cut(s) 185, 202, 346, 725, 847
NlaIII CATG 3 cut(s) 151, 217, 500
NlaIV GGNNCC 2 cut(s) 519, 942
NmuCI GTSAC 1 cut(s) 506
PfeI GAWTC 6 cut(s) 35, 334, 439, 707, 787, 830
PflFI GACNNNGTC 1 cut(s) 215
PkrI GCNGC 6 cut(s) 301, 474, 477, 682, 780, 928
PleI GAGTC 1 cut(s) 171
PpsI GAGTC 1 cut(s) 171
Psp124BI GAGCTC 1 cut(s) 370
Psp6I CCWGG 1 cut(s) 342
PspEI GGTNACC 1 cut(s) 506
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 2 cut(s) 519, 942
PspPI GGNCC 2 cut(s) 379, 940
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 2 cut(s) 308, 761
PsuI RGATCY 2 cut(s) 185, 346
PsyI GACNNNGTC 1 cut(s) 215
PvuII CAGCTG 1 cut(s) 299
RsaI GTAC 3 cut(s) 265, 694, 859
RsaNI GTAC 3 cut(s) 264, 693, 858
RseI CAYNNNNRTG 1 cut(s) 197
SacI GAGCTC 1 cut(s) 370
SacII CCGCGG 1 cut(s) 779
SaqAI TTAA 4 cut(s) 288, 572, 594, 668
SatI GCNGC 6 cut(s) 300, 473, 476, 681, 779, 927
Sau3AI GATC 5 cut(s) 185, 202, 346, 725, 847
Sau96I GGNCC 2 cut(s) 379, 940
SchI GAGTC 1 cut(s) 171
ScrFI CCNGG 1 cut(s) 344
SduI GDGCHC 1 cut(s) 370
SfaNI GCATC 5 cut(s) 114, 628, 640, 692, 938
SfcI CTRYAG 3 cut(s) 154, 300, 350
Sfr303I CCGCGG 1 cut(s) 779
SgrBI CCGCGG 1 cut(s) 779
SinI GGWCC 1 cut(s) 940
SmiMI CAYNNNNRTG 1 cut(s) 197
SmlI CTYRAG 2 cut(s) 76, 137
SmoI CTYRAG 2 cut(s) 76, 137
Sse9I AATT 3 cut(s) 411, 569, 821
SsiI CCGC 4 cut(s) 406, 776, 778, 926
SspI AATATT 1 cut(s) 133
SspMI CTAG 1 cut(s) 281
SstI GAGCTC 1 cut(s) 370
StyD4I CCNGG 1 cut(s) 342
TaaI ACNGT 5 cut(s) 310, 400, 427, 469, 757
TaqI TCGA 4 cut(s) 12, 180, 546, 720
TasI AATT 3 cut(s) 411, 569, 821
TatI WGTACW 1 cut(s) 263
TauI GCSGC 2 cut(s) 781, 929
TfiI GAWTC 6 cut(s) 35, 334, 439, 707, 787, 830
Tru1I TTAA 4 cut(s) 288, 572, 594, 668
Tru9I TTAA 4 cut(s) 288, 572, 594, 668
TscAI CASTG 3 cut(s) 37, 682, 762
TseFI GTSAC 1 cut(s) 506
TseI GCWGC 4 cut(s) 299, 472, 475, 680
Tsp45I GTSAC 1 cut(s) 506
TspDTI ATGAA 5 cut(s) 164, 336, 819, 834, 915
TspRI CASTG 3 cut(s) 37, 682, 762
Tth111I GACNNNGTC 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 940
XmiI GTMKAC 2 cut(s) 402, 628
XspI CTAG 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.