RchiOBHm_Chr3g0488591

regulation of parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
N/A
Physical Location & Seq
Reverse (-)
36245281 .. 36246490
1210 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 408 bp
ATGCAGGAAGAGTTAAATCAATTTGCTAGGAATGATGTATGGTACTTGGTACCTAGACTTGGAAATTGCAATGTCATAGGCACTAAGTGGATCTTCAGAAACAAAAGTGATGAAAAAGGGAATGTAATTAGAAACAAAGCTAGGTTAGTAGCTCAGGGTTACTCTCAGGTTGAAGGTCTTGATTTTGATGAAACCTTTGCTCATGTTGCTAGATTAGAATTAGTGAGGTTACTCTTGTCTGTAGCCTGTCATTTCAGGTTCAAACTGTTTCAAATGGATGTCAAAACTGCCTTCCTGAATGAGATCTTGCAGGAAGAAGTTTATGTGGAACAACCTCCGAGTTTCAAAGATCCACATAACTTAGATCATGTCTACCGACTCAAGAAAGCCTTGTATGGGCTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Chorein_N Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

135

Amino Acids

15.87

Weight (kDa)

8.55

Isoelectric Point (pI)

31.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 11 - 135 1.7e-37 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 49
AccB1I GGYRCC 1 cut(s) 49
AccI GTMKAC 1 cut(s) 372
AclWI GGATC 2 cut(s) 98, 344
AcuI CTGAAG 1 cut(s) 79
AdeI CACNNNGTG 1 cut(s) 87
AfaI GTAC 2 cut(s) 44, 51
AfiI CCNNNNNNNGG 2 cut(s) 59, 396
AgsI TTSAA 4 cut(s) 173, 262, 272, 346
AluBI AGCT 2 cut(s) 140, 152
AluI AGCT 2 cut(s) 140, 152
AlwI GGATC 2 cut(s) 98, 344
Asp718I GGTACC 1 cut(s) 49
BanI GGYRCC 1 cut(s) 49
BfaI CTAG 4 cut(s) 27, 54, 141, 210
BfmI CTRYAG 1 cut(s) 240
BglII AGATCT 1 cut(s) 303
BmiI GGNNCC 1 cut(s) 51
Bpu10I CCTNAGC 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 365
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 396
Bse3DI GCAATG 1 cut(s) 76
BseGI GGATG 1 cut(s) 283
BseLI CCNNNNNNNGG 2 cut(s) 59, 396
BseMI GCAATG 1 cut(s) 76
BseMII CTCAG 2 cut(s) 167, 179
BshNI GGYRCC 1 cut(s) 49
BslI CCNNNNNNNGG 2 cut(s) 59, 396
Bsp143I GATC 4 cut(s) 90, 303, 349, 364
BspCNI CTCAG 2 cut(s) 166, 178
BspLI GGNNCC 1 cut(s) 51
BspPI GGATC 2 cut(s) 98, 344
BspT107I GGYRCC 1 cut(s) 49
BsrDI GCAATG 1 cut(s) 76
BssMI GATC 4 cut(s) 90, 303, 349, 364
Bst4CI ACNGT 1 cut(s) 267
Bst6I CTCTTC 1 cut(s) 3
BstDEI CTNAG 4 cut(s) 84, 153, 165, 361
BstF5I GGATG 1 cut(s) 283
BstKTI GATC 4 cut(s) 93, 306, 352, 367
BstMBI GATC 4 cut(s) 90, 303, 349, 364
BstMWI GCNNNNNNNGC 1 cut(s) 206
BstSFI CTRYAG 1 cut(s) 240
BstX2I RGATCY 3 cut(s) 90, 303, 349
BstYI RGATCY 3 cut(s) 90, 303, 349
BtsCI GGATG 1 cut(s) 283
Csp6I GTAC 2 cut(s) 43, 50
CviAII CATG 2 cut(s) 203, 368
CviJI RGCY 5 cut(s) 140, 152, 245, 389, 400
CviKI_1 RGCY 5 cut(s) 140, 152, 245, 389, 400
CviQI GTAC 2 cut(s) 43, 50
DdeI CTNAG 4 cut(s) 84, 153, 165, 361
DpnI GATC 4 cut(s) 92, 305, 351, 366
DpnII GATC 4 cut(s) 90, 303, 349, 364
DraIII CACNNNGTG 1 cut(s) 87
Eam1104I CTCTTC 1 cut(s) 3
EarI CTCTTC 1 cut(s) 3
Eco57I CTGAAG 1 cut(s) 79
FaeI CATG 2 cut(s) 206, 371
FaiI YATR 7 cut(s) 40, 77, 204, 324, 357, 369, 396
FalI AAGNNNNNCTT 4 cut(s) 77, 109, 374, 406
FatI CATG 2 cut(s) 202, 367
FblI GTMKAC 1 cut(s) 372
FokI GGATG 1 cut(s) 290
FspBI CTAG 4 cut(s) 27, 54, 141, 210
Hin1II CATG 2 cut(s) 206, 371
HinfI GANTC 1 cut(s) 378
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 2 cut(s) 98, 339
Hpy188III TCNNGA 3 cut(s) 179, 295, 382
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 2 cut(s) 167, 301
HpyCH4III ACNGT 1 cut(s) 267
HpyCH4V TGCA 3 cut(s) 4, 69, 310
HpyF10VI GCNNNNNNNGC 1 cut(s) 206
HpyF3I CTNAG 4 cut(s) 84, 153, 165, 361
Hsp92II CATG 2 cut(s) 206, 371
KpnI GGTACC 1 cut(s) 53
Kzo9I GATC 4 cut(s) 90, 303, 349, 364
LpnPI CCDG 6 cut(s) 140, 152, 241, 259, 296, 308
MaeI CTAG 4 cut(s) 27, 54, 141, 210
MaeIII GTNAC 2 cut(s) 158, 228
MalI GATC 4 cut(s) 92, 305, 351, 366
MboI GATC 4 cut(s) 90, 303, 349, 364
MboII GAAGA 3 cut(s) 20, 85, 326
MflI RGATCY 3 cut(s) 90, 303, 349
MluCI AATT 4 cut(s) 20, 64, 126, 218
MlyI GAGTC 1 cut(s) 372
MnlI CCTC 2 cut(s) 219, 345
MseI TTAA 1 cut(s) 14
MwoI GCNNNNNNNGC 1 cut(s) 206
NdeII GATC 4 cut(s) 90, 303, 349, 364
NlaIII CATG 2 cut(s) 206, 371
NlaIV GGNNCC 1 cut(s) 51
PleI GAGTC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 372
PspN4I GGNNCC 1 cut(s) 51
PsuI RGATCY 3 cut(s) 90, 303, 349
RsaI GTAC 2 cut(s) 44, 51
RsaNI GTAC 2 cut(s) 43, 50
SaqAI TTAA 1 cut(s) 14
Sau3AI GATC 4 cut(s) 90, 303, 349, 364
SchI GAGTC 1 cut(s) 372
SfcI CTRYAG 1 cut(s) 240
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
Sse9I AATT 4 cut(s) 20, 64, 126, 218
SspMI CTAG 4 cut(s) 27, 54, 141, 210
TaaI ACNGT 1 cut(s) 267
TasI AATT 4 cut(s) 20, 64, 126, 218
Tru1I TTAA 1 cut(s) 14
Tru9I TTAA 1 cut(s) 14
TspDTI ATGAA 2 cut(s) 126, 204
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 4 cut(s) 27, 54, 141, 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.