RchiOBHm_Chr2g0096981

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0029702
Physical Location & Seq
Forward (+)
9713032 .. 9713562
531 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 459 bp
ATGACGACTAGAATAGCTCCTGGAGTCGGAGCCAACTTGCTCGGTCAACACTCCACCGAGAGAAACCAAGACGCTACTCTTTACGTCCGCAATCTCGACCCTCAGAGAGTGACAAACTTGCATCAAGGATACAGATTGGTGGAATTTCGAAGTGAAGAGGATGCTAGTTACGCAATCAAGGTGCTTAATATGATCAAGCTTTATGGAAAGCCAATTCGTGTAAATAAGGCTTCCCAAGATAAAAAGAGCTTGGATGTAGGGGCAAACCTTTTCGTCGGAAATCTTGATCCTGATGTGGATGAGAAACTTCTCTATGATACTTTCAGTGCATTTGGTGTCATAGTTACAAACCCAAAGATAATGAGAGATCCTGAAACTGGAAACTCGTGTGGATTTGGCTTCATCAGTTATGATTCCTTTGAGGCATCTGATGCAGCTATCGAGGCAATGTATGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
RRM_1

Protein Analysis

152

Amino Acids

16.83

Weight (kDa)

5.14

Isoelectric Point (pI)

23.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 90 - 152 1.6e-16 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029702)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0096981
rosa_samantha Rh2DG113500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 88
AclWI GGATC 2 cut(s) 281, 362
AcsI RAATTY 1 cut(s) 143
AfiI CCNNNNNNNGG 2 cut(s) 26, 377
AjnI CCWGG 1 cut(s) 19
AluBI AGCT 4 cut(s) 17, 199, 249, 437
AluI AGCT 4 cut(s) 17, 199, 249, 437
AlwI GGATC 2 cut(s) 281, 362
ApeKI GCWGC 1 cut(s) 434
ApoI RAATTY 1 cut(s) 143
AsuII TTCGAA 1 cut(s) 148
BauI CACGAG 1 cut(s) 385
BbvI GCAGC 1 cut(s) 446
BciT130I CCWGG 1 cut(s) 21
BciVI GTATCC 1 cut(s) 122
BclI TGATCA 1 cut(s) 192
BfaI CTAG 3 cut(s) 9, 165, 457
BfuI GTATCC 1 cut(s) 122
BisI GCNGC 1 cut(s) 435
BlsI GCNGC 1 cut(s) 436
Bme1390I CCNGG 1 cut(s) 21
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 1 cut(s) 21
BmsI GCATC 4 cut(s) 130, 151, 421, 434
BpmI CTGGAG 1 cut(s) 42
Bpu14I TTCGAA 1 cut(s) 148
Bsc4I CCNNNNNNNGG 2 cut(s) 26, 377
Bse1I ACTGG 1 cut(s) 382
Bse3DI GCAATG 1 cut(s) 453
BseBI CCWGG 1 cut(s) 21
BseGI GGATG 3 cut(s) 166, 259, 304
BseLI CCNNNNNNNGG 2 cut(s) 26, 377
BseMI GCAATG 1 cut(s) 453
BseMII CTCAG 1 cut(s) 116
BseNI ACTGG 1 cut(s) 382
BseXI GCAGC 1 cut(s) 446
BslI CCNNNNNNNGG 2 cut(s) 26, 377
Bsp119I TTCGAA 1 cut(s) 148
Bsp143I GATC 3 cut(s) 192, 286, 367
BspACI CCGC 1 cut(s) 88
BspCNI CTCAG 1 cut(s) 115
BspLI GGNNCC 1 cut(s) 31
BspPI GGATC 2 cut(s) 281, 362
BspT104I TTCGAA 1 cut(s) 148
BsrDI GCAATG 1 cut(s) 453
BsrI ACTGG 1 cut(s) 382
BssMI GATC 3 cut(s) 192, 286, 367
BssSI CACGAG 1 cut(s) 385
Bst2BI CACGAG 1 cut(s) 385
Bst2UI CCWGG 1 cut(s) 21
Bst6I CTCTTC 1 cut(s) 150
BstAPI GCANNNNNTGC 1 cut(s) 431
BstBI TTCGAA 1 cut(s) 148
BstDEI CTNAG 1 cut(s) 102
BstF5I GGATG 3 cut(s) 166, 259, 304
BstKTI GATC 3 cut(s) 195, 289, 370
BstMBI GATC 3 cut(s) 192, 286, 367
BstMWI GCNNNNNNNGC 3 cut(s) 170, 431, 443
BstNI CCWGG 1 cut(s) 21
BstSCI CCNGG 1 cut(s) 19
BstV1I GCAGC 1 cut(s) 446
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
BsuI GTATCC 1 cut(s) 122
BtsCI GGATG 3 cut(s) 166, 259, 304
BtsIMutI CAGTG 1 cut(s) 331
CseI GACGC 1 cut(s) 80
CviJI RGCY 9 cut(s) 17, 32, 199, 211, 230, 249, 399, 437, 456
CviKI_1 RGCY 9 cut(s) 17, 32, 199, 211, 230, 249, 399, 437, 456
DdeI CTNAG 1 cut(s) 102
DpnI GATC 3 cut(s) 194, 288, 369
DpnII GATC 3 cut(s) 192, 286, 367
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
EcoRII CCWGG 1 cut(s) 19
FaiI YATR 6 cut(s) 191, 204, 315, 341, 411, 453
FbaI TGATCA 1 cut(s) 192
Fnu4HI GCNGC 1 cut(s) 435
FokI GGATG 3 cut(s) 173, 266, 311
Fsp4HI GCNGC 1 cut(s) 435
FspBI CTAG 3 cut(s) 9, 165, 457
GluI GCNGC 1 cut(s) 435
GsuI CTGGAG 1 cut(s) 42
HgaI GACGC 1 cut(s) 80
HincII GTYRAC 1 cut(s) 47
HindII GTYRAC 1 cut(s) 47
HindIII AAGCTT 1 cut(s) 197
HinfI GANTC 2 cut(s) 24, 413
Hpy166II GTNNAC 1 cut(s) 47
Hpy188I TCNGA 4 cut(s) 29, 105, 278, 430
Hpy188III TCNNGA 4 cut(s) 95, 284, 290, 371
Hpy8I GTNNAC 1 cut(s) 47
Hpy99I CGWCG 1 cut(s) 278
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 3 cut(s) 121, 329, 434
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 431, 443
HpyF3I CTNAG 1 cut(s) 102
HpySE526I ACGT 1 cut(s) 84
Ksp22I TGATCA 1 cut(s) 192
Kzo9I GATC 3 cut(s) 192, 286, 367
LmnI GCTCC 2 cut(s) 22, 29
LpnPI CCDG 5 cut(s) 6, 33, 303, 363, 384
Lsp1109I GCAGC 1 cut(s) 446
LweI GCATC 4 cut(s) 130, 151, 421, 434
MaeI CTAG 3 cut(s) 9, 165, 457
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 3 cut(s) 109, 167, 343
MalI GATC 3 cut(s) 194, 288, 369
MboI GATC 3 cut(s) 192, 286, 367
MboII GAAGA 1 cut(s) 167
MflI RGATCY 1 cut(s) 367
MluCI AATT 2 cut(s) 143, 213
MlyI GAGTC 1 cut(s) 33
MmeI TCCRAC 2 cut(s) 7, 256
MnlI CCTC 4 cut(s) 111, 151, 415, 436
MseI TTAA 1 cut(s) 186
MspR9I CCNGG 1 cut(s) 21
MvaI CCWGG 1 cut(s) 21
MwoI GCNNNNNNNGC 3 cut(s) 170, 431, 443
NdeII GATC 3 cut(s) 192, 286, 367
NlaIV GGNNCC 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 109
NspV TTCGAA 1 cut(s) 148
PfeI GAWTC 1 cut(s) 413
PfoI TCCNGGA 1 cut(s) 19
PkrI GCNGC 1 cut(s) 436
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
Psp6I CCWGG 1 cut(s) 19
PspGI CCWGG 1 cut(s) 19
PspN4I GGNNCC 1 cut(s) 31
PsuI RGATCY 1 cut(s) 367
SaqAI TTAA 1 cut(s) 186
SatI GCNGC 1 cut(s) 435
Sau3AI GATC 3 cut(s) 192, 286, 367
SchI GAGTC 1 cut(s) 33
ScrFI CCNGG 1 cut(s) 21
SetI ASST 7 cut(s) 19, 87, 183, 201, 251, 270, 439
SfaNI GCATC 4 cut(s) 130, 151, 421, 434
SfuI TTCGAA 1 cut(s) 148
Sse9I AATT 2 cut(s) 143, 213
SsiI CCGC 1 cut(s) 88
SspMI CTAG 3 cut(s) 9, 165, 457
StyD4I CCNGG 1 cut(s) 19
TaiI ACGT 1 cut(s) 87
TaqI TCGA 3 cut(s) 96, 148, 441
TaqII GACCGA 1 cut(s) 32
TasI AATT 2 cut(s) 143, 213
TfiI GAWTC 1 cut(s) 413
Tru1I TTAA 1 cut(s) 186
Tru9I TTAA 1 cut(s) 186
TscAI CASTG 1 cut(s) 331
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 1 cut(s) 434
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 1 cut(s) 391
TspRI CASTG 1 cut(s) 331
XapI RAATTY 1 cut(s) 143
XspI CTAG 3 cut(s) 9, 165, 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.