RchiOBHm_Chr2g0104921

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
N/A
Physical Location & Seq
Reverse (-)
16168389 .. 16172989
4601 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1857 bp
ATGCCGACTAGCTTGGAAAAAAGAAAGATGGTGACAGTGCTAAGCATTGATGGAGGTGGAATTAAAGGAATCATACCCGGCACCCTTATTGCCTTTCTTGAAACCAAGCTTCAGGATTTGGATGGTCCGAGTGCAAGACTTGCAGATTATTTTGATATAATTGCCGGAATAAGCACAGGGGGGTTGGTCACTACCATGCTTACAGCTCCCAACAAGGACAATCGACCCATGTATGAAGCAAAGGACATCAACAACTTCTACTTTGAACAGACACCCAAGATCTTCCCTCAAAATAGGTCAGCAAAAGAAAGTTCTCTGAAAAATCCAAAGCTTTCGGATATCTGTATCAGTACCTCTGCAGCTCCAACTTTTCTCCCCGCACACTACTTCGAGGTCAAGGACAGCCAGGGAACAACCCGTACTTTTGATCTCGTCGATGGTGGGGTTGCCGCAAACAACCCTACAATGATGGCCATAAGCCACATAAACAGAGAGATGTTGAAGCACGGCTCAGAACCAATGGATGCTAATCGGTTGCTAGTGCTATCATTGGGCACAGGCACTGCCAAGTTCGAAGGGAAGTACAGTGCAACCAAGGCCTCAAAATGGGGTTTGATCAAAATATCAAACAAAAGAAAAACCGAAAAAGGAAAGAGGAAAAAGAATAAAGAAAAAGAGCATCCACTTAGTTGGCATATAATGTCGATTGGTTTGGCAAAGAGAAAGATGGTGACAGTTCTGAGCATTGATGGAGGGGGCATTAGAGGCATAATTCCCAGCACCATCCTCGGCTTTCTCGAATCTAAGCTTCAGGATTTGGATGGAGCAAATGCAAGAATTGCAGATTACTTTGACATAATCGCCGGAACTAGCACTGGTGGGTTGGTCACCACCATGCTTACAGCTCCCAACAAGAACAATCGACCCATGTATGAGGCAAAGGACATCAACAACTTTTATTTAGAACACTGTCCCCAGATTTTCCCTCAAAAAAGTCGGAAAAACTTCTTCACATCGATCACAAATGCAACGAAACCGGGCCCAAAATACGATGGGAAGTATCTGCGCTCATTGACAAACGGGCTGCTTGGTGACCTCACTCTCAAGCAGACTTTAACCAATGTGGTCATTCCAACTTTTGATATCAAACACCTTCAGCCTGTGGTTTTCACAACCATTGACGCAAAAGAAAATTCATTGAAGAACGCAAAGCTTTCAGATGTCTGCATCAGTACCTCTGCAGCTCCATCTTATCTCCCCCCACACTACTTTGAAGTCAAGGATGATCAGGGAAATATCCGCACGTTTGATCTTGTCGATGGTGGGGTTGCTGCAAACAACCCTACAATGATGGCCATAAGCCACATAAACAGAGAGATGTTGAAGAACAACTCAGAACCGATGGATGCGACTCGGTTGCAAGTGCTGTCATTGGGCACCGGTGCTGCCAAGTATGCAGAGAAGTACAGTGCAGTCGACGCATCAAAATGGGGTTTGGTGAATTGGATGTTTCATAATGGGAGCACACCTTTGCTGGACATTTTTGGCGATGCAAGTTCTGATATGGTTGACTTTCATGTCTCCACTCTCTTCCAGTCCCTCAATGTCAAGGACAATTACCTAAGAATTCAGGATGACACATTGAGCGGTGAAGAATCATCAGTGGATATTGCAACCGAGAAGAATCTGGAAAGCCTTGTGGTGATCGGAAACGGGTTGCTAAAGAAGCCAGTGTCAAGGGTGAATATGGATACAGGGAGGTATGAGAAATGTGAGGGAGAAGCCACCTATGAAGAAGCACTAGTCCATTTTGCCATACAGCTCTCGGAGGAAAGGAAGCTCAGACAAACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

618

Amino Acids

68.06

Weight (kDa)

8.64

Isoelectric Point (pI)

35.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 14 - 90 1.5e-07 Patatin-like phospholipase
Patatin PF01734 90 - 159 1.5e-06 Patatin-like phospholipase
Patatin PF01734 247 - 453 2.2e-27 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1577
AccB1I GGYRCC 2 cut(s) 80, 1436
AccBSI CCGCTC 1 cut(s) 1647
AccI GTMKAC 1 cut(s) 1476
AciI CCGC 4 cut(s) 378, 450, 1300, 1647
AcoI YGGCCR 2 cut(s) 471, 1353
AcsI RAATTY 2 cut(s) 1192, 1626
AcuI CTGAAG 3 cut(s) 95, 794, 1139
AfaI GTAC 5 cut(s) 352, 421, 584, 1234, 1466
AfiI CCNNNNNNNGG 1 cut(s) 606
AgeI ACCGGT 1 cut(s) 1439
AgsI TTSAA 6 cut(s) 101, 266, 502, 1201, 1274, 1384
AhlI ACTAGT 1 cut(s) 1801
AjnI CCWGG 1 cut(s) 405
Alw21I GWGCWC 1 cut(s) 1526
Alw26I GTCTC 1 cut(s) 1585
AlwNI CAGNNNCTG 1 cut(s) 563
AoxI GGCC 4 cut(s) 471, 597, 1039, 1353
ApaI GGGCCC 1 cut(s) 1043
ApeKI GCWGC 5 cut(s) 359, 1084, 1241, 1331, 1445
ApoI RAATTY 2 cut(s) 1192, 1626
AsiGI ACCGGT 1 cut(s) 1439
Asp700I GAANNNNTTC 1 cut(s) 1004
AspLEI GCGC 1 cut(s) 1068
AspS9I GGNCC 3 cut(s) 125, 1039, 1040
AsuC2I CCSGG 2 cut(s) 78, 1038
AsuHPI GGTGA 8 cut(s) 43, 742, 880, 1103, 1510, 1661, 1714, 1753
AsuII TTCGAA 1 cut(s) 573
AvaII GGWCC 1 cut(s) 125
BaeGI GKGCMC 3 cut(s) 557, 1043, 1439
BalI TGGCCA 2 cut(s) 473, 1355
BanI GGYRCC 2 cut(s) 80, 1436
BanII GRGCYC 1 cut(s) 1043
Bbv12I GWGCWC 1 cut(s) 1526
BbvI GCAGC 5 cut(s) 371, 1071, 1253, 1318, 1432
BceAI ACGGC 1 cut(s) 523
BcgI CGANNNNNNTGC 4 cut(s) 769, 803, 1399, 1433
BciT130I CCWGG 1 cut(s) 407
BciVI GTATCC 1 cut(s) 1744
BclI TGATCA 2 cut(s) 615, 1285
BcnI CCSGG 2 cut(s) 78, 1038
BcoDI GTCTC 1 cut(s) 1585
BcuI ACTAGT 1 cut(s) 1801
BfaI CTAG 4 cut(s) 9, 539, 870, 1802
BfmI CTRYAG 2 cut(s) 357, 1239
BfuI GTATCC 1 cut(s) 1744
BglII AGATCT 1 cut(s) 279
BisI GCNGC 6 cut(s) 360, 450, 1085, 1242, 1332, 1446
BlpI GCTNAGC 1 cut(s) 41
BlsI GCNGC 6 cut(s) 361, 451, 1086, 1243, 1333, 1447
Bme1390I CCNGG 3 cut(s) 78, 407, 1038
Bme18I GGWCC 1 cut(s) 125
BmgT120I GGNCC 3 cut(s) 125, 1039, 1040
BmiI GGNNCC 3 cut(s) 82, 1041, 1438
BmrFI CCNGG 3 cut(s) 78, 407, 1038
BmsI GCATC 6 cut(s) 514, 688, 1236, 1396, 1490, 1540
Bpu1102I GCTNAGC 1 cut(s) 41
Bpu14I TTCGAA 1 cut(s) 573
BpuEI CTTGAG 1 cut(s) 1088
BpuMI CCSGG 2 cut(s) 78, 1038
Bsa29I ATCGAT 1 cut(s) 1016
BsaBI GATNNNNATC 1 cut(s) 528
BsaJI CCNNGG 3 cut(s) 406, 594, 787
BsaWI WCCGGW 1 cut(s) 1439
Bsc4I CCNNNNNNNGG 1 cut(s) 606
Bse118I RCCGGY 1 cut(s) 1439
Bse1I ACTGG 3 cut(s) 880, 1594, 1730
Bse8I GATNNNNATC 1 cut(s) 528
BseBI CCWGG 1 cut(s) 407
BseCI ATCGAT 1 cut(s) 1016
BseDI CCNNGG 3 cut(s) 406, 594, 787
BseGI GGATG 9 cut(s) 127, 529, 679, 783, 826, 1288, 1411, 1512, 1639
BseJI GATNNNNATC 1 cut(s) 528
BseLI CCNNNNNNNGG 1 cut(s) 606
BseMII CTCAG 4 cut(s) 525, 731, 1407, 1855
BseNI ACTGG 3 cut(s) 880, 1594, 1730
BseSI GKGCMC 3 cut(s) 557, 1043, 1439
BseXI GCAGC 5 cut(s) 371, 1071, 1253, 1318, 1432
BseYI CCCAGC 1 cut(s) 776
BsgI GTGCAG 1 cut(s) 1491
BshFI GGCC 4 cut(s) 473, 599, 1041, 1355
BshNI GGYRCC 2 cut(s) 80, 1436
BshTI ACCGGT 1 cut(s) 1439
BshVI ATCGAT 1 cut(s) 1016
BsiHKAI GWGCWC 1 cut(s) 1526
BsiSI CCGG 5 cut(s) 78, 165, 864, 1037, 1440
BslFI GGGAC 2 cut(s) 957, 1582
BslI CCNNNNNNNGG 1 cut(s) 606
BsmAI GTCTC 1 cut(s) 1585
BsmFI GGGAC 2 cut(s) 957, 1582
BsnI GGCC 4 cut(s) 473, 599, 1041, 1355
Bsp119I TTCGAA 1 cut(s) 573
Bsp120I GGGCCC 1 cut(s) 1039
Bsp1286I GDGCHC 4 cut(s) 557, 1043, 1439, 1526
Bsp143I GATC 7 cut(s) 279, 427, 615, 1017, 1285, 1309, 1704
Bsp1720I GCTNAGC 1 cut(s) 41
BspACI CCGC 4 cut(s) 378, 450, 1300, 1647
BspANI GGCC 4 cut(s) 473, 599, 1041, 1355
BspCNI CTCAG 4 cut(s) 524, 732, 1406, 1854
BspDI ATCGAT 1 cut(s) 1016
BspLI GGNNCC 3 cut(s) 82, 1041, 1438
BspMAI CTGCAG 2 cut(s) 361, 1243
BspT104I TTCGAA 1 cut(s) 573
BspT107I GGYRCC 2 cut(s) 80, 1436
BsrBI CCGCTC 1 cut(s) 1647
BsrFI RCCGGY 1 cut(s) 1439
BsrI ACTGG 3 cut(s) 880, 1594, 1730
BssAI RCCGGY 1 cut(s) 1439
BssECI CCNNGG 3 cut(s) 406, 594, 787
BssMI GATC 7 cut(s) 279, 427, 615, 1017, 1285, 1309, 1704
BssT1I CCWWGG 1 cut(s) 594
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 5 cut(s) 37, 587, 736, 971, 1469
Bst6I CTCTTC 1 cut(s) 1595
BstAPI GCANNNNNTGC 2 cut(s) 140, 839
BstBI TTCGAA 1 cut(s) 573
BstDEI CTNAG 8 cut(s) 41, 511, 686, 740, 804, 1393, 1622, 1841
BstEII GGTNACC 2 cut(s) 886, 1091
BstF5I GGATG 9 cut(s) 127, 529, 679, 783, 826, 1288, 1411, 1512, 1639
BstHHI GCGC 1 cut(s) 1068
BstKTI GATC 7 cut(s) 282, 430, 618, 1020, 1288, 1312, 1707
BstMAI GTCTC 1 cut(s) 1585
BstMBI GATC 7 cut(s) 279, 427, 615, 1017, 1285, 1309, 1704
BstMWI GCNNNNNNNGC 7 cut(s) 140, 596, 765, 839, 1454, 1478, 1726
BstNI CCWGG 1 cut(s) 407
BstPI GGTNACC 2 cut(s) 886, 1091
BstSCI CCNGG 3 cut(s) 76, 405, 1036
BstSFI CTRYAG 2 cut(s) 357, 1239
BstSLI GKGCMC 3 cut(s) 557, 1043, 1439
BstV1I GCAGC 5 cut(s) 371, 1071, 1253, 1318, 1432
BstX2I RGATCY 1 cut(s) 279
BstXI CCANNNNNNTGG 1 cut(s) 690
BstYI RGATCY 1 cut(s) 279
Bsu15I ATCGAT 1 cut(s) 1016
BsuI GTATCC 1 cut(s) 1744
BsuRI GGCC 4 cut(s) 473, 599, 1041, 1355
BsuTUI ATCGAT 1 cut(s) 1016
BtgZI GCGATG 1 cut(s) 1563
BtsCI GGATG 9 cut(s) 127, 529, 679, 783, 826, 1288, 1411, 1512, 1639
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 8 cut(s) 42, 561, 592, 873, 967, 1474, 1668, 1737
CaiI CAGNNNCTG 1 cut(s) 563
CfoI GCGC 1 cut(s) 1068
Cfr10I RCCGGY 1 cut(s) 1439
Cfr13I GGNCC 3 cut(s) 125, 1039, 1040
ClaI ATCGAT 1 cut(s) 1016
CseI GACGC 2 cut(s) 1190, 1487
Csp6I GTAC 5 cut(s) 351, 420, 583, 1233, 1465
CspAI ACCGGT 1 cut(s) 1439
CviAII CATG 5 cut(s) 196, 229, 895, 928, 1577
CviQI GTAC 5 cut(s) 351, 420, 583, 1233, 1465
DdeI CTNAG 8 cut(s) 41, 511, 686, 740, 804, 1393, 1622, 1841
DpnI GATC 7 cut(s) 281, 429, 617, 1019, 1287, 1311, 1706
DpnII GATC 7 cut(s) 279, 427, 615, 1017, 1285, 1309, 1704
DrdI GACNNNNNNGTC 1 cut(s) 1577
DseDI GACNNNNNNGTC 1 cut(s) 1577
EaeI YGGCCR 2 cut(s) 471, 1353
Eam1104I CTCTTC 1 cut(s) 1595
EarI CTCTTC 1 cut(s) 1595
Eco130I CCWWGG 1 cut(s) 594
Eco147I AGGCCT 1 cut(s) 599
Eco24I GRGCYC 1 cut(s) 1043
Eco32I GATATC 2 cut(s) 340, 1144
Eco47I GGWCC 1 cut(s) 125
Eco57I CTGAAG 3 cut(s) 95, 794, 1139
Eco91I GGTNACC 2 cut(s) 886, 1091
EcoO65I GGTNACC 2 cut(s) 886, 1091
EcoRI GAATTC 1 cut(s) 1626
EcoRII CCWGG 1 cut(s) 405
EcoRV GATATC 2 cut(s) 340, 1144
EcoT14I CCWWGG 1 cut(s) 594
EcoT38I GRGCYC 1 cut(s) 1043
ErhI CCWWGG 1 cut(s) 594
FaeI CATG 5 cut(s) 199, 232, 898, 931, 1580
FaqI GGGAC 2 cut(s) 957, 1582
FatI CATG 5 cut(s) 195, 228, 894, 927, 1576
FauI CCCGC 1 cut(s) 385
FbaI TGATCA 2 cut(s) 615, 1285
FblI GTMKAC 1 cut(s) 1476
Fnu4HI GCNGC 6 cut(s) 360, 450, 1085, 1242, 1332, 1446
FokI GGATG 9 cut(s) 134, 536, 666, 770, 833, 1295, 1418, 1519, 1646
FriOI GRGCYC 1 cut(s) 1043
Fsp4HI GCNGC 6 cut(s) 360, 450, 1085, 1242, 1332, 1446
FspBI CTAG 4 cut(s) 9, 539, 870, 1802
GlaI GCGC 1 cut(s) 1067
GluI GCNGC 6 cut(s) 360, 450, 1085, 1242, 1332, 1446
GsaI CCCAGC 1 cut(s) 780
HaeIII GGCC 4 cut(s) 473, 599, 1041, 1355
HapII CCGG 5 cut(s) 78, 165, 864, 1037, 1440
HgaI GACGC 2 cut(s) 1190, 1487
HhaI GCGC 1 cut(s) 1068
Hin1II CATG 5 cut(s) 199, 232, 898, 931, 1580
Hin6I GCGC 1 cut(s) 1066
HinP1I GCGC 1 cut(s) 1066
HincII GTYRAC 2 cut(s) 1477, 1570
HindII GTYRAC 2 cut(s) 1477, 1570
HindIII AAGCTT 4 cut(s) 107, 329, 806, 1211
HinfI GANTC 5 cut(s) 69, 800, 1411, 1655, 1684
HpaII CCGG 5 cut(s) 78, 165, 864, 1037, 1440
HphI GGTGA 8 cut(s) 43, 742, 880, 1103, 1510, 1661, 1714, 1753
Hpy166II GTNNAC 2 cut(s) 1477, 1570
Hpy188III TCNNGA 6 cut(s) 98, 113, 797, 812, 1631, 1688
Hpy8I GTNNAC 2 cut(s) 1477, 1570
Hpy99I CGWCG 2 cut(s) 437, 1481
HpyAV CCTTC 2 cut(s) 569, 1163
HpyCH4III ACNGT 5 cut(s) 37, 587, 736, 971, 1469
HpyCH4IV ACGT 1 cut(s) 1304
HpyF10VI GCNNNNNNNGC 7 cut(s) 140, 596, 765, 839, 1454, 1478, 1726
HpyF3I CTNAG 8 cut(s) 41, 511, 686, 740, 804, 1393, 1622, 1841
HpySE526I ACGT 1 cut(s) 1304
Hsp92II CATG 5 cut(s) 199, 232, 898, 931, 1580
HspAI GCGC 1 cut(s) 1066
Ksp22I TGATCA 2 cut(s) 615, 1285
Kzo9I GATC 7 cut(s) 279, 427, 615, 1017, 1285, 1309, 1704
LmnI GCTCC 6 cut(s) 211, 367, 824, 910, 1249, 1521
Lsp1109I GCAGC 5 cut(s) 371, 1071, 1253, 1318, 1432
LweI GCATC 6 cut(s) 514, 688, 1236, 1396, 1490, 1540
MaeI CTAG 4 cut(s) 9, 539, 870, 1802
MaeII ACGT 1 cut(s) 1304
MaeIII GTNAC 5 cut(s) 31, 187, 730, 886, 1091
MalI GATC 7 cut(s) 281, 429, 617, 1019, 1287, 1311, 1706
MbiI CCGCTC 1 cut(s) 1647
MboI GATC 7 cut(s) 279, 427, 615, 1017, 1285, 1309, 1704
MboII GAAGA 8 cut(s) 274, 1000, 1213, 1396, 1582, 1664, 1693, 1805
MfeI CAATTG 1 cut(s) 1852
MflI RGATCY 1 cut(s) 279
MhlI GDGCHC 4 cut(s) 557, 1043, 1439, 1526
MlsI TGGCCA 2 cut(s) 473, 1355
MluCI AATT 9 cut(s) 60, 159, 771, 837, 1192, 1501, 1615, 1626, 1852
MluNI TGGCCA 2 cut(s) 473, 1355
MlyI GAGTC 1 cut(s) 1405
MmeI TCCRAC 3 cut(s) 389, 977, 1157
Mox20I TGGCCA 2 cut(s) 473, 1355
MroXI GAANNNNTTC 1 cut(s) 1004
MscI TGGCCA 2 cut(s) 473, 1355
MseI TTAA 2 cut(s) 63, 1115
MslI CAYNNNNRTG 3 cut(s) 194, 893, 1486
Msp20I TGGCCA 2 cut(s) 473, 1355
MspI CCGG 5 cut(s) 78, 165, 864, 1037, 1440
MspR9I CCNGG 3 cut(s) 78, 407, 1038
MunI CAATTG 1 cut(s) 1852
MvaI CCWGG 1 cut(s) 407
MwoI GCNNNNNNNGC 7 cut(s) 140, 596, 765, 839, 1454, 1478, 1726
NciI CCSGG 2 cut(s) 78, 1038
NdeII GATC 7 cut(s) 279, 427, 615, 1017, 1285, 1309, 1704
NlaIII CATG 5 cut(s) 199, 232, 898, 931, 1580
NlaIV GGNNCC 3 cut(s) 82, 1041, 1438
NmeAIII GCCGAG 1 cut(s) 768
NmuCI GTSAC 5 cut(s) 31, 187, 730, 886, 1091
NspV TTCGAA 1 cut(s) 573
PceI AGGCCT 1 cut(s) 599
PcsI WCGNNNNNNNCGW 1 cut(s) 795
PdmI GAANNNNTTC 1 cut(s) 1004
PfeI GAWTC 4 cut(s) 69, 800, 1655, 1684
PinAI ACCGGT 1 cut(s) 1439
PkrI GCNGC 6 cut(s) 361, 451, 1086, 1243, 1333, 1447
PleI GAGTC 1 cut(s) 1405
PpsI GAGTC 1 cut(s) 1405
Psp6I CCWGG 1 cut(s) 405
PspEI GGTNACC 2 cut(s) 886, 1091
PspFI CCCAGC 1 cut(s) 776
PspGI CCWGG 1 cut(s) 405
PspN4I GGNNCC 3 cut(s) 82, 1041, 1438
PspOMI GGGCCC 1 cut(s) 1039
PspPI GGNCC 3 cut(s) 125, 1039, 1040
PsrI GAACNNNNNNTAC 2 cut(s) 403, 435
PstI CTGCAG 2 cut(s) 361, 1243
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 1 cut(s) 279
RsaI GTAC 5 cut(s) 352, 421, 584, 1234, 1466
RsaNI GTAC 5 cut(s) 351, 420, 583, 1233, 1465
RseI CAYNNNNRTG 3 cut(s) 194, 893, 1486
SalI GTCGAC 1 cut(s) 1475
SaqAI TTAA 2 cut(s) 63, 1115
SatI GCNGC 6 cut(s) 360, 450, 1085, 1242, 1332, 1446
Sau3AI GATC 7 cut(s) 279, 427, 615, 1017, 1285, 1309, 1704
Sau96I GGNCC 3 cut(s) 125, 1039, 1040
SchI GAGTC 1 cut(s) 1405
ScrFI CCNGG 3 cut(s) 78, 407, 1038
SduI GDGCHC 4 cut(s) 557, 1043, 1439, 1526
SfaNI GCATC 6 cut(s) 514, 688, 1236, 1396, 1490, 1540
SfcI CTRYAG 2 cut(s) 357, 1239
SfuI TTCGAA 1 cut(s) 573
SgrAI CRCCGGYG 1 cut(s) 1439
SinI GGWCC 1 cut(s) 125
SmiMI CAYNNNNRTG 3 cut(s) 194, 893, 1486
SmlI CTYRAG 1 cut(s) 1103
SmoI CTYRAG 1 cut(s) 1103
SpeI ACTAGT 1 cut(s) 1801
Sse9I AATT 9 cut(s) 60, 159, 771, 837, 1192, 1501, 1615, 1626, 1852
SseBI AGGCCT 1 cut(s) 599
SsiI CCGC 4 cut(s) 378, 450, 1300, 1647
SspMI CTAG 4 cut(s) 9, 539, 870, 1802
StuI AGGCCT 1 cut(s) 599
StyD4I CCNGG 3 cut(s) 76, 405, 1036
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 5 cut(s) 37, 587, 736, 971, 1469
TaiI ACGT 1 cut(s) 1307
TasI AATT 9 cut(s) 60, 159, 771, 837, 1192, 1501, 1615, 1626, 1852
TatI WGTACW 2 cut(s) 582, 1464
TauI GCSGC 1 cut(s) 452
TfiI GAWTC 4 cut(s) 69, 800, 1655, 1684
Tru1I TTAA 2 cut(s) 63, 1115
Tru9I TTAA 2 cut(s) 63, 1115
TscAI CASTG 8 cut(s) 42, 568, 592, 880, 974, 1474, 1668, 1737
TseFI GTSAC 5 cut(s) 31, 187, 730, 886, 1091
TseI GCWGC 5 cut(s) 359, 1084, 1241, 1331, 1445
Tsp45I GTSAC 5 cut(s) 31, 187, 730, 886, 1091
TspDTI ATGAA 5 cut(s) 249, 1185, 1502, 1565, 1806
TspRI CASTG 8 cut(s) 42, 568, 592, 880, 974, 1474, 1668, 1737
VpaK11BI GGWCC 1 cut(s) 125
XapI RAATTY 2 cut(s) 1192, 1626
XmiI GTMKAC 1 cut(s) 1476
XmnI GAANNNNTTC 1 cut(s) 1004
XspI CTAG 4 cut(s) 9, 539, 870, 1802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.