RchiOBHm_Chr2g0109691

PRONE (Plant-specific Rop nucleotide exchanger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0029834
Physical Location & Seq
Forward (+)
21045749 .. 21046225
477 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 351 bp
ATGAAGGAGAGATTTGCAAAGCTTCTACTTGGAGAAGATATGTCAGGTAGAGGCAATGGAGTCTGCACTGCTCTTGCTATTTCCAGTGCCATCACAAATCTTTCTGCTACTATGTTTGGAGAGTTATGGAAGTTGGAGCCATTGCCACCACAGAAGAAGGCAATGTGGTGCCGAGAAATGGAATGGCTTTTGTGTGTGAGTGATTCGATTGTTGAGCTCATACCATCAACTCAAGAATTCCCTGGTGGTGGGACTTTTGAGGTTATGGTCACTTGTCCCCGCTCAGATCTCTATGTGAGCCTACCTACCCTCAAGAAACTGGATGCAATGCTGCTTAGCCTTCTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRONE

Protein Analysis

116

Amino Acids

12.81

Weight (kDa)

4.95

Isoelectric Point (pI)

32.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRONE PF03759 1 - 116 1.3e-58 PRONE (Plant-specific Rop nucleotide exchanger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0029834)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0109691
rosa_samantha Rh2CG216600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 168
AccBSI CCGCTC 1 cut(s) 282
AciI CCGC 1 cut(s) 280
AcsI RAATTY 1 cut(s) 236
AfiI CCNNNNNNNGG 2 cut(s) 178, 248
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 2 cut(s) 22, 217
AluI AGCT 2 cut(s) 22, 217
Alw21I GWGCWC 1 cut(s) 219
ApeKI GCWGC 1 cut(s) 331
ApoI RAATTY 1 cut(s) 236
BanI GGYRCC 1 cut(s) 168
BanII GRGCYC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 1 cut(s) 318
BccI CCATC 2 cut(s) 98, 232
BciT130I CCWGG 1 cut(s) 243
BglII AGATCT 1 cut(s) 286
BisI GCNGC 1 cut(s) 332
BlpI GCTNAGC 1 cut(s) 335
BlsI GCNGC 1 cut(s) 333
Bme1390I CCNGG 1 cut(s) 243
BmiI GGNNCC 2 cut(s) 138, 170
BmrFI CCNGG 1 cut(s) 243
BmsI GCATC 1 cut(s) 313
Bpu1102I GCTNAGC 1 cut(s) 335
BpuEI CTTGAG 2 cut(s) 216, 296
BsaJI CCNNGG 1 cut(s) 241
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 248
Bse1I ACTGG 2 cut(s) 84, 324
Bse3DI GCAATG 4 cut(s) 61, 140, 168, 333
BseBI CCWGG 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 241
BseGI GGATG 1 cut(s) 328
BseLI CCNNNNNNNGG 2 cut(s) 178, 248
BseMI GCAATG 4 cut(s) 61, 140, 168, 333
BseMII CTCAG 1 cut(s) 297
BseNI ACTGG 2 cut(s) 84, 324
BseXI GCAGC 1 cut(s) 318
BsgI GTGCAG 1 cut(s) 49
BshNI GGYRCC 1 cut(s) 168
BsiHKAI GWGCWC 1 cut(s) 219
BslFI GGGAC 2 cut(s) 261, 265
BslI CCNNNNNNNGG 2 cut(s) 178, 248
BsmFI GGGAC 2 cut(s) 261, 265
Bsp1286I GDGCHC 1 cut(s) 219
Bsp143I GATC 1 cut(s) 286
Bsp1720I GCTNAGC 1 cut(s) 335
BspACI CCGC 1 cut(s) 280
BspCNI CTCAG 1 cut(s) 296
BspLI GGNNCC 2 cut(s) 138, 170
BspT107I GGYRCC 1 cut(s) 168
BsrBI CCGCTC 1 cut(s) 282
BsrDI GCAATG 4 cut(s) 61, 140, 168, 333
BsrI ACTGG 2 cut(s) 84, 324
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 1 cut(s) 286
Bst2UI CCWGG 1 cut(s) 243
BstDEI CTNAG 2 cut(s) 283, 335
BstF5I GGATG 1 cut(s) 328
BstKTI GATC 1 cut(s) 289
BstMBI GATC 1 cut(s) 286
BstNI CCWGG 1 cut(s) 243
BstSCI CCNGG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 318
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
BtsCI GGATG 1 cut(s) 328
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 2 cut(s) 66, 91
CviJI RGCY 6 cut(s) 22, 139, 187, 217, 300, 339
CviKI_1 RGCY 6 cut(s) 22, 139, 187, 217, 300, 339
DdeI CTNAG 2 cut(s) 283, 335
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
Ecl136II GAGCTC 1 cut(s) 217
Eco24I GRGCYC 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 217
EcoICRI GAGCTC 1 cut(s) 217
EcoRI GAATTC 1 cut(s) 236
EcoRII CCWGG 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 219
FaiI YATR 6 cut(s) 41, 113, 127, 221, 266, 294
FaqI GGGAC 2 cut(s) 261, 265
FauI CCCGC 1 cut(s) 287
Fnu4HI GCNGC 1 cut(s) 332
FokI GGATG 1 cut(s) 335
FriOI GRGCYC 1 cut(s) 219
Fsp4HI GCNGC 1 cut(s) 332
GluI GCNGC 1 cut(s) 332
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 2 cut(s) 60, 203
Hpy188I TCNGA 1 cut(s) 286
Hpy188III TCNNGA 3 cut(s) 233, 313, 344
HpyAV CCTTC 2 cut(s) 151, 350
HpyCH4V TGCA 3 cut(s) 17, 66, 326
HpyF3I CTNAG 2 cut(s) 283, 335
Kzo9I GATC 1 cut(s) 286
LmnI GCTCC 1 cut(s) 136
LpnPI CCDG 6 cut(s) 30, 97, 228, 255, 305, 329
Lsp1109I GCAGC 1 cut(s) 318
LweI GCATC 1 cut(s) 313
MaeIII GTNAC 1 cut(s) 268
MalI GATC 1 cut(s) 288
MbiI CCGCTC 1 cut(s) 282
MboI GATC 1 cut(s) 286
MboII GAAGA 2 cut(s) 47, 166
MflI RGATCY 1 cut(s) 286
MhlI GDGCHC 1 cut(s) 219
MluCI AATT 1 cut(s) 236
MlyI GAGTC 1 cut(s) 69
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 3 cut(s) 44, 253, 320
MspR9I CCNGG 1 cut(s) 243
MvaI CCWGG 1 cut(s) 243
NdeII GATC 1 cut(s) 286
NlaIV GGNNCC 2 cut(s) 138, 170
NmeAIII GCCGAG 1 cut(s) 197
NmuCI GTSAC 1 cut(s) 268
PfeI GAWTC 1 cut(s) 203
PkrI GCNGC 1 cut(s) 333
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
Psp124BI GAGCTC 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 241
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 2 cut(s) 138, 170
PsuI RGATCY 1 cut(s) 286
SacI GAGCTC 1 cut(s) 219
SatI GCNGC 1 cut(s) 332
Sau3AI GATC 1 cut(s) 286
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 1 cut(s) 243
SduI GDGCHC 1 cut(s) 219
SetI ASST 5 cut(s) 24, 49, 219, 264, 307
SfaNI GCATC 1 cut(s) 313
SmlI CTYRAG 2 cut(s) 231, 311
SmoI CTYRAG 2 cut(s) 231, 311
Sse9I AATT 1 cut(s) 236
SsiI CCGC 1 cut(s) 280
SstI GAGCTC 1 cut(s) 219
StyD4I CCNGG 1 cut(s) 241
TaqI TCGA 1 cut(s) 206
TasI AATT 1 cut(s) 236
TfiI GAWTC 1 cut(s) 203
TscAI CASTG 2 cut(s) 73, 91
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 1 cut(s) 331
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 73, 91
XapI RAATTY 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.