RchiOBHm_Chr2g0113571
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
N/A
Physical Location & Seq
Forward (+)
25688845 .. 25692744
3900 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 408 bp
ATGTTTCTCTTCGACTGGTTCTACGGCGTGCTCGCTTCCCTCGGTCTATGGCAGAAGGAGGCCAAGATCTTGTTCCTAGGGCTCGATAATGCTGGCAAGACCACCTTGCTTCACATGCTCAAAGATGATTCCACAGCAGAGGCCAAGTCTAAACTTATCACGGGGGCACCAGTAATTAATCAAAACAAACAGCTTAGTCCGCAGCTCAGAATACAAATAGTTTACTTGAAAACAATCCAAATCCACCTAGTGAAAGACTTGAGTTTATGCCCAAAAAGTTCAATATTTCGCATGAATTTCCATGGCGGTACGAGGCTGGTGCAGCATCAACCTACGCAATATCCCACATCCGAGGAGTTGAGTATTGGCAAAATTAAGTTCAAGGCCTTTGATTTGGGAGGTCATTAG

Protein Analysis

135

Amino Acids

15.23

Weight (kDa)

9.55

Isoelectric Point (pI)

29.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 47 2.5e-11 ADP-ribosylation factor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 166
AciI CCGC 2 cut(s) 200, 306
AcsI RAATTY 1 cut(s) 295
AdeI CACNNNGTG 1 cut(s) 250
AfaI GTAC 1 cut(s) 310
AgsI TTSAA 3 cut(s) 229, 282, 382
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 2 cut(s) 193, 205
AluI AGCT 2 cut(s) 193, 205
Alw21I GWGCWC 1 cut(s) 33
AoxI GGCC 3 cut(s) 60, 141, 384
ApeKI GCWGC 2 cut(s) 202, 322
ApoI RAATTY 1 cut(s) 295
AseI ATTAAT 1 cut(s) 177
AspA2I CCTAGG 1 cut(s) 76
AvrII CCTAGG 1 cut(s) 76
BaeGI GKGCMC 1 cut(s) 169
BanI GGYRCC 1 cut(s) 166
BanII GRGCYC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 2 cut(s) 214, 334
BceAI ACGGC 1 cut(s) 40
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BfaI CTAG 2 cut(s) 77, 248
BglII AGATCT 1 cut(s) 66
BisI GCNGC 2 cut(s) 203, 323
BlnI CCTAGG 1 cut(s) 76
BlsI GCNGC 2 cut(s) 204, 324
BmiI GGNNCC 1 cut(s) 168
BmsI GCATC 1 cut(s) 334
BpuEI CTTGAG 1 cut(s) 280
BsaJI CCNNGG 4 cut(s) 40, 76, 301, 351
Bse1I ACTGG 2 cut(s) 20, 170
BseDI CCNNGG 4 cut(s) 40, 76, 301, 351
BseGI GGATG 1 cut(s) 347
BseMII CTCAG 1 cut(s) 220
BseNI ACTGG 2 cut(s) 20, 170
BseRI GAGGAG 1 cut(s) 368
BseSI GKGCMC 1 cut(s) 169
BseXI GCAGC 2 cut(s) 214, 334
BsgI GTGCAG 1 cut(s) 341
BshFI GGCC 3 cut(s) 62, 143, 386
BshNI GGYRCC 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 33
BsnI GGCC 3 cut(s) 62, 143, 386
Bsp1286I GDGCHC 3 cut(s) 33, 84, 169
Bsp143I GATC 1 cut(s) 66
Bsp19I CCATGG 1 cut(s) 301
BspACI CCGC 2 cut(s) 200, 306
BspANI GGCC 3 cut(s) 62, 143, 386
BspCNI CTCAG 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 168
BspT107I GGYRCC 1 cut(s) 166
BsrI ACTGG 2 cut(s) 20, 170
BssECI CCNNGG 4 cut(s) 40, 76, 301, 351
BssMI GATC 1 cut(s) 66
BssT1I CCWWGG 2 cut(s) 76, 301
Bst6I CTCTTC 1 cut(s) 14
BstC8I GCNNGC 3 cut(s) 29, 33, 94
BstDEI CTNAG 2 cut(s) 194, 206
BstDSI CCRYGG 1 cut(s) 301
BstF5I GGATG 1 cut(s) 347
BstKTI GATC 1 cut(s) 69
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 3 cut(s) 115, 199, 322
BstNSI RCATGY 1 cut(s) 118
BstSLI GKGCMC 1 cut(s) 169
BstV1I GCAGC 2 cut(s) 214, 334
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuRI GGCC 3 cut(s) 62, 143, 386
BtgI CCRYGG 1 cut(s) 301
BtsCI GGATG 1 cut(s) 347
Cac8I GCNNGC 3 cut(s) 29, 33, 94
Csp6I GTAC 1 cut(s) 309
CviAII CATG 3 cut(s) 115, 292, 302
CviJI RGCY 7 cut(s) 62, 82, 143, 193, 205, 316, 386
CviKI_1 RGCY 7 cut(s) 62, 82, 143, 193, 205, 316, 386
CviQI GTAC 1 cut(s) 309
DdeI CTNAG 2 cut(s) 194, 206
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
DraIII CACNNNGTG 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Eco130I CCWWGG 2 cut(s) 76, 301
Eco147I AGGCCT 1 cut(s) 386
Eco24I GRGCYC 1 cut(s) 84
EcoT14I CCWWGG 2 cut(s) 76, 301
EcoT38I GRGCYC 1 cut(s) 84
ErhI CCWWGG 2 cut(s) 76, 301
FaeI CATG 3 cut(s) 118, 295, 305
FaiI YATR 5 cut(s) 49, 116, 268, 293, 303
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FatI CATG 3 cut(s) 114, 291, 301
Fnu4HI GCNGC 2 cut(s) 203, 323
FokI GGATG 1 cut(s) 334
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 2 cut(s) 203, 323
FspBI CTAG 2 cut(s) 77, 248
GluI GCNGC 2 cut(s) 203, 323
HaeIII GGCC 3 cut(s) 62, 143, 386
Hin1II CATG 3 cut(s) 118, 295, 305
HinfI GANTC 1 cut(s) 128
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 2 cut(s) 209, 352
Hpy8I GTNNAC 1 cut(s) 223
HpyAV CCTTC 1 cut(s) 49
HpyCH4V TGCA 1 cut(s) 322
HpyF10VI GCNNNNNNNGC 3 cut(s) 115, 199, 322
HpyF3I CTNAG 2 cut(s) 194, 206
Hsp92II CATG 3 cut(s) 118, 295, 305
Kzo9I GATC 1 cut(s) 66
LpnPI CCDG 3 cut(s) 78, 183, 302
Lsp1109I GCAGC 2 cut(s) 214, 334
LweI GCATC 1 cut(s) 334
MaeI CTAG 2 cut(s) 77, 248
MalI GATC 1 cut(s) 68
MboI GATC 1 cut(s) 66
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 3 cut(s) 33, 84, 169
MluCI AATT 3 cut(s) 174, 295, 372
MnlI CCTC 6 cut(s) 50, 52, 133, 306, 346, 392
MseI TTAA 2 cut(s) 177, 375
MwoI GCNNNNNNNGC 3 cut(s) 115, 199, 322
NcoI CCATGG 1 cut(s) 301
NdeII GATC 1 cut(s) 66
NlaIII CATG 3 cut(s) 118, 295, 305
NlaIV GGNNCC 1 cut(s) 168
NspI RCATGY 1 cut(s) 118
PceI AGGCCT 1 cut(s) 386
PfeI GAWTC 1 cut(s) 128
PkrI GCNGC 2 cut(s) 204, 324
PshBI ATTAAT 1 cut(s) 177
PspN4I GGNNCC 1 cut(s) 168
PsuI RGATCY 1 cut(s) 66
RsaI GTAC 1 cut(s) 310
RsaNI GTAC 1 cut(s) 309
SaqAI TTAA 2 cut(s) 177, 375
SatI GCNGC 2 cut(s) 203, 323
Sau3AI GATC 1 cut(s) 66
SduI GDGCHC 3 cut(s) 33, 84, 169
SetI ASST 6 cut(s) 107, 195, 207, 249, 334, 403
SfaNI GCATC 1 cut(s) 334
SmlI CTYRAG 1 cut(s) 259
SmoI CTYRAG 1 cut(s) 259
Sse9I AATT 3 cut(s) 174, 295, 372
SseBI AGGCCT 1 cut(s) 386
SsiI CCGC 2 cut(s) 200, 306
SspI AATATT 1 cut(s) 285
SspMI CTAG 2 cut(s) 77, 248
StuI AGGCCT 1 cut(s) 386
StyI CCWWGG 2 cut(s) 76, 301
TaqI TCGA 2 cut(s) 12, 84
TaqII GACCGA 1 cut(s) 32
TasI AATT 3 cut(s) 174, 295, 372
TfiI GAWTC 1 cut(s) 128
Tru1I TTAA 2 cut(s) 177, 375
Tru9I TTAA 2 cut(s) 177, 375
TseI GCWGC 2 cut(s) 202, 322
TspDTI ATGAA 1 cut(s) 308
VspI ATTAAT 1 cut(s) 177
XapI RAATTY 1 cut(s) 295
XceI RCATGY 1 cut(s) 118
XmaJI CCTAGG 1 cut(s) 76
XspI CTAG 2 cut(s) 77, 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.