RchiOBHm_Chr2g0116861

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
N/A
Physical Location & Seq
Reverse (-)
29114507 .. 29115590
1084 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 501 bp
ATGGAGCCACGGTTAGTATCTATTGAATTTCAAACTTTGAGTTTTACAAACTCAAAAGACATTAGAGTTGAAGGATTATCATTGAACAACCAAATGTTTCACATTGTGATAAACGGCTGCCATGATGTACTCATTCAAGGCGTCAAAATTATTGCTGCTGGCAATAGCCCTAACACCGACGGCATTCATGTCCAATTTTCCAAAAATGTTGCAATCTTCGACACCTCCATCAAAACCAGGGATGATTGTGTCTCTATTGGCCCCGGCGCCAAGGACTTGTGGATGGAACGCATATCATGCGGACCTGATCATGGCATTAGAACATACGAGAACATGCTTGAAGCAGTTGCTGTAAAATTTGATTGCAGCGCAACAAATCCCTGCACTAAGATAAGACTCGAAAACGTGAATTTGACTTGCCGGAAGCAAGTAGTTCAATCAATTTGTACAAATGTCAATGGTCAGAGTGTTGGCAGTGTTCATCCGGACAGTTGTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

166

Amino Acids

18.19

Weight (kDa)

6.18

Isoelectric Point (pI)

27.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 12 - 106 8.2e-25 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 266
AccIII TCCGGA 1 cut(s) 484
AciI CCGC 1 cut(s) 300
AcsI RAATTY 3 cut(s) 26, 356, 409
AcyI GRCGYC 2 cut(s) 141, 267
AdeI CACNNNGTG 1 cut(s) 106
AfaI GTAC 2 cut(s) 129, 448
AfiI CCNNNNNNNGG 1 cut(s) 311
AgsI TTSAA 7 cut(s) 26, 32, 71, 85, 137, 341, 437
AjnI CCWGG 1 cut(s) 236
Alw26I GTCTC 1 cut(s) 256
AlwNI CAGNNNCTG 1 cut(s) 350
Aor13HI TCCGGA 1 cut(s) 484
AoxI GGCC 1 cut(s) 259
ApeKI GCWGC 3 cut(s) 117, 155, 366
ApoI RAATTY 3 cut(s) 26, 356, 409
AspLEI GCGC 2 cut(s) 269, 371
AspS9I GGNCC 2 cut(s) 260, 302
AsuC2I CCSGG 1 cut(s) 264
AvaII GGWCC 1 cut(s) 302
BanI GGYRCC 1 cut(s) 266
BbvI GCAGC 3 cut(s) 104, 142, 378
BccI CCATC 2 cut(s) 236, 277
BceAI ACGGC 2 cut(s) 130, 196
BciT130I CCWGG 1 cut(s) 238
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 1 cut(s) 264
BcoDI GTCTC 1 cut(s) 256
BfoI RGCGCY 1 cut(s) 270
BisI GCNGC 3 cut(s) 118, 156, 367
BlsI GCNGC 3 cut(s) 119, 157, 368
Bme1390I CCNGG 2 cut(s) 238, 264
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 2 cut(s) 260, 302
BmiI GGNNCC 3 cut(s) 6, 262, 268
BmrFI CCNGG 2 cut(s) 238, 264
BoxI GACNNNNGTC 1 cut(s) 492
BpuMI CCSGG 1 cut(s) 264
BsaHI GRCGYC 2 cut(s) 141, 267
BsaJI CCNNGG 4 cut(s) 8, 237, 262, 270
BsaWI WCCGGW 1 cut(s) 484
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 311
BseAI TCCGGA 1 cut(s) 484
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 4 cut(s) 8, 237, 262, 270
BseGI GGATG 3 cut(s) 247, 288, 481
BseLI CCNNNNNNNGG 1 cut(s) 311
BseXI GCAGC 3 cut(s) 104, 142, 378
BsgI GTGCAG 1 cut(s) 367
BshFI GGCC 1 cut(s) 261
BshNI GGYRCC 1 cut(s) 266
BsiSI CCGG 3 cut(s) 264, 421, 485
BslI CCNNNNNNNGG 1 cut(s) 311
BsmAI GTCTC 1 cut(s) 256
BsmI GAATGC 1 cut(s) 183
BsnI GGCC 1 cut(s) 261
Bsp13I TCCGGA 1 cut(s) 484
Bsp1407I TGTACA 1 cut(s) 446
Bsp143I GATC 1 cut(s) 307
BspACI CCGC 1 cut(s) 300
BspANI GGCC 1 cut(s) 261
BspEI TCCGGA 1 cut(s) 484
BspLI GGNNCC 3 cut(s) 6, 262, 268
BspT107I GGYRCC 1 cut(s) 266
BsrGI TGTACA 1 cut(s) 446
BssECI CCNNGG 4 cut(s) 8, 237, 262, 270
BssMI GATC 1 cut(s) 307
BssNI GRCGYC 2 cut(s) 141, 267
BssT1I CCWWGG 1 cut(s) 270
Bst2UI CCWGG 1 cut(s) 238
Bst4CI ACNGT 2 cut(s) 12, 491
BstACI GRCGYC 2 cut(s) 141, 267
BstAPI GCANNNNNTGC 1 cut(s) 297
BstAUI TGTACA 1 cut(s) 446
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 1 cut(s) 387
BstDSI CCRYGG 1 cut(s) 8
BstF5I GGATG 3 cut(s) 247, 288, 481
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 2 cut(s) 269, 371
BstKTI GATC 1 cut(s) 310
BstMAI GTCTC 1 cut(s) 256
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 1 cut(s) 297
BstNI CCWGG 1 cut(s) 238
BstNSI RCATGY 1 cut(s) 337
BstPAI GACNNNNGTC 1 cut(s) 492
BstSCI CCNGG 2 cut(s) 236, 262
BstV1I GCAGC 3 cut(s) 104, 142, 378
BsuRI GGCC 1 cut(s) 261
BtgI CCRYGG 1 cut(s) 8
BtsCI GGATG 3 cut(s) 247, 288, 481
BtsI GCAGTG 1 cut(s) 481
BtsIMutI CAGTG 1 cut(s) 481
Cac8I GCNNGC 1 cut(s) 160
CaiI CAGNNNCTG 1 cut(s) 350
CfoI GCGC 2 cut(s) 269, 371
Cfr13I GGNCC 2 cut(s) 260, 302
CseI GACGC 1 cut(s) 130
Csp6I GTAC 2 cut(s) 128, 447
CviAII CATG 5 cut(s) 122, 188, 297, 311, 334
CviJI RGCY 4 cut(s) 7, 117, 168, 261
CviKI_1 RGCY 4 cut(s) 7, 117, 168, 261
CviQI GTAC 2 cut(s) 128, 447
DdeI CTNAG 1 cut(s) 387
DinI GGCGCC 1 cut(s) 268
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
DraIII CACNNNGTG 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 302
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 270
EgeI GGCGCC 1 cut(s) 268
EheI GGCGCC 1 cut(s) 268
ErhI CCWWGG 1 cut(s) 270
FaeI CATG 5 cut(s) 125, 191, 300, 314, 337
FaiI YATR 7 cut(s) 123, 189, 293, 298, 312, 325, 335
FatI CATG 5 cut(s) 121, 187, 296, 310, 333
FbaI TGATCA 1 cut(s) 307
Fnu4HI GCNGC 3 cut(s) 118, 156, 367
FokI GGATG 3 cut(s) 254, 295, 468
Fsp4HI GCNGC 3 cut(s) 118, 156, 367
GlaI GCGC 2 cut(s) 268, 370
GluI GCNGC 3 cut(s) 118, 156, 367
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 1 cut(s) 261
HapII CCGG 3 cut(s) 264, 421, 485
HgaI GACGC 1 cut(s) 130
HhaI GCGC 2 cut(s) 269, 371
Hin1I GRCGYC 2 cut(s) 141, 267
Hin1II CATG 5 cut(s) 125, 191, 300, 314, 337
Hin6I GCGC 2 cut(s) 267, 369
HinP1I GCGC 2 cut(s) 267, 369
HinfI GANTC 1 cut(s) 396
HpaII CCGG 3 cut(s) 264, 421, 485
Hpy188I TCNGA 1 cut(s) 465
Hpy188III TCNNGA 1 cut(s) 485
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 1 cut(s) 65
HpyCH4III ACNGT 2 cut(s) 12, 491
HpyCH4IV ACGT 1 cut(s) 405
HpyCH4V TGCA 3 cut(s) 212, 366, 384
HpyF10VI GCNNNNNNNGC 1 cut(s) 297
HpyF3I CTNAG 1 cut(s) 387
HpySE526I ACGT 1 cut(s) 405
Hsp92I GRCGYC 2 cut(s) 141, 267
Hsp92II CATG 5 cut(s) 125, 191, 300, 314, 337
HspAI GCGC 2 cut(s) 267, 369
KasI GGCGCC 1 cut(s) 266
Kpn2I TCCGGA 1 cut(s) 484
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 7 cut(s) 144, 223, 250, 277, 318, 394, 434
Lsp1109I GCAGC 3 cut(s) 104, 142, 378
MaeII ACGT 1 cut(s) 405
MalI GATC 1 cut(s) 309
MboI GATC 1 cut(s) 307
MboII GAAGA 1 cut(s) 208
MluCI AATT 6 cut(s) 26, 147, 194, 356, 409, 441
Mly113I GGCGCC 1 cut(s) 267
MlyI GAGTC 1 cut(s) 390
MnlI CCTC 1 cut(s) 235
MroI TCCGGA 1 cut(s) 484
MspI CCGG 3 cut(s) 264, 421, 485
MspR9I CCNGG 2 cut(s) 238, 264
Mva1269I GAATGC 1 cut(s) 183
MvaI CCWGG 1 cut(s) 238
MwoI GCNNNNNNNGC 1 cut(s) 297
NarI GGCGCC 1 cut(s) 267
NciI CCSGG 1 cut(s) 264
NdeII GATC 1 cut(s) 307
NlaIII CATG 5 cut(s) 125, 191, 300, 314, 337
NlaIV GGNNCC 3 cut(s) 6, 262, 268
NspI RCATGY 1 cut(s) 337
PctI GAATGC 1 cut(s) 183
PkrI GCNGC 3 cut(s) 119, 157, 368
PleI GAGTC 1 cut(s) 390
PluTI GGCGCC 1 cut(s) 270
PpsI GAGTC 1 cut(s) 390
PshAI GACNNNNGTC 1 cut(s) 492
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PspN4I GGNNCC 3 cut(s) 6, 262, 268
PspPI GGNCC 2 cut(s) 260, 302
PstNI CAGNNNCTG 1 cut(s) 350
RsaI GTAC 2 cut(s) 129, 448
RsaNI GTAC 2 cut(s) 128, 447
SatI GCNGC 3 cut(s) 118, 156, 367
Sau3AI GATC 1 cut(s) 307
Sau96I GGNCC 2 cut(s) 260, 302
SchI GAGTC 1 cut(s) 390
ScrFI CCNGG 2 cut(s) 238, 264
SetI ASST 3 cut(s) 227, 307, 408
SfoI GGCGCC 1 cut(s) 268
SinI GGWCC 1 cut(s) 302
Sse9I AATT 6 cut(s) 26, 147, 194, 356, 409, 441
SsiI CCGC 1 cut(s) 300
SspDI GGCGCC 1 cut(s) 266
StyD4I CCNGG 2 cut(s) 236, 262
StyI CCWWGG 1 cut(s) 270
TaaI ACNGT 2 cut(s) 12, 491
TaiI ACGT 1 cut(s) 408
TaqI TCGA 2 cut(s) 219, 399
TasI AATT 6 cut(s) 26, 147, 194, 356, 409, 441
TatI WGTACW 2 cut(s) 127, 446
TscAI CASTG 1 cut(s) 481
TseI GCWGC 3 cut(s) 117, 155, 366
TspDTI ATGAA 2 cut(s) 176, 470
TspRI CASTG 1 cut(s) 481
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 3 cut(s) 26, 356, 409
XceI RCATGY 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.