RchiOBHm_Chr2g0123841

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0029859
Physical Location & Seq
Reverse (-)
37118085 .. 37120169
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 669 bp
ATGGGTGCATGGACTTTGAGTACCCCGCTCGTAGGAATTTATAGCATCATAAATCATTATCCAAGCATATTCAAGGCCATATCACCACACTATATCTTCCGTTTCTTTTGGAGAAACGGTAAGGAAGGCTGGTTGTTGCTTAGTGGAACTGTCCTTTGCATCACAGGGTCCGAGGCATTGTTTGCAGATCTTGGTCATTTCAACCGAAGCTCCATTCAGATAGCTTTCTTGTTTACCATCTATCCTTCTCTGGTTCTGACATATGCGGGGCAGACAGCATACCTGATCAAGAATCCAAATGATCATAATGATGGATTCTATAAGTTTATACCAACTACTATCTACTGGCCTATCTTTATCATATCTACATTAGCTGCAATAGTTGCAAGCCAGTCTCTGATATCAGCCACGTTTTCGGTAATCAAGCAATCTGTAGTACTTGATTATTTCCCTCGAGTGAAGGTTGTGCACACATCCCCTAGCAAAGAAGGCGAGGTTTACTCCCCTGAAGTCAATTACATTCTCATGGTTCTCTGTGTTGCTGTCATACTTATATTTGGGGATGGAAAAGACATAGGAAATGCATTTGGTGTTGTTGTCAGCTTAGTCATGCTCATCACCACAATATTACTAACACCGCTACATCAAAGATTACATCCAAGAACCTGA

Protein Analysis

222

Amino Acids

24.77

Weight (kDa)

8.83

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K_trans PF02705 2 - 214 1.3e-78 K+ potassium transporter integral membrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0029859)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0123841
rosa_samantha Rh2BG315800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 28
AciI CCGC 3 cut(s) 26, 266, 638
AcsI RAATTY 1 cut(s) 36
AcuI CTGAAG 1 cut(s) 528
AfaI GTAC 2 cut(s) 22, 438
AfiI CCNNNNNNNGG 1 cut(s) 32
AgsI TTSAA 2 cut(s) 73, 202
AluBI AGCT 4 cut(s) 210, 224, 374, 603
AluI AGCT 4 cut(s) 210, 224, 374, 603
Alw21I GWGCWC 1 cut(s) 471
Alw26I GTCTC 1 cut(s) 399
Alw44I GTGCAC 1 cut(s) 467
AlwNI CAGNNNCTG 1 cut(s) 397
Ama87I CYCGRG 1 cut(s) 453
AoxI GGCC 2 cut(s) 75, 347
ApaLI GTGCAC 1 cut(s) 467
ApeKI GCWGC 1 cut(s) 374
ApoI RAATTY 1 cut(s) 36
AspS9I GGNCC 1 cut(s) 168
AsuHPI GGTGA 2 cut(s) 75, 610
AvaI CYCGRG 1 cut(s) 453
AvaII GGWCC 1 cut(s) 168
BaeGI GKGCMC 1 cut(s) 471
Bbv12I GWGCWC 1 cut(s) 471
BbvI GCAGC 1 cut(s) 361
BccI CCATC 3 cut(s) 245, 305, 557
BclI TGATCA 2 cut(s) 285, 301
BcoDI GTCTC 1 cut(s) 399
BfaI CTAG 1 cut(s) 480
BfmI CTRYAG 1 cut(s) 432
BglII AGATCT 1 cut(s) 187
BisI GCNGC 1 cut(s) 375
BlsI GCNGC 1 cut(s) 376
BmcAI AGTACT 1 cut(s) 438
Bme18I GGWCC 1 cut(s) 168
BmeT110I CYCGRG 1 cut(s) 453
BmgT120I GGNCC 1 cut(s) 168
BmiI GGNNCC 1 cut(s) 169
BmsI GCATC 2 cut(s) 54, 168
BplI GAGNNNNNCTC 2 cut(s) 485, 517
BsaJI CCNNGG 1 cut(s) 171
BsaXI ACNNNNNCTCC 4 cut(s) 103, 133, 194, 224
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse1I ACTGG 2 cut(s) 350, 391
BseDI CCNNGG 1 cut(s) 171
BseGI GGATG 3 cut(s) 473, 568, 655
BseLI CCNNNNNNNGG 1 cut(s) 32
BseNI ACTGG 2 cut(s) 350, 391
BseSI GKGCMC 1 cut(s) 471
BseXI GCAGC 1 cut(s) 361
BshFI GGCC 2 cut(s) 77, 349
BsiHKAI GWGCWC 1 cut(s) 471
BsiHKCI CYCGRG 1 cut(s) 453
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 1 cut(s) 399
BsnI GGCC 2 cut(s) 77, 349
BsoBI CYCGRG 1 cut(s) 453
Bsp1286I GDGCHC 1 cut(s) 471
Bsp143I GATC 3 cut(s) 187, 285, 301
BspACI CCGC 3 cut(s) 26, 266, 638
BspANI GGCC 2 cut(s) 77, 349
BspLI GGNNCC 1 cut(s) 169
BsrBI CCGCTC 1 cut(s) 28
BsrI ACTGG 2 cut(s) 350, 391
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 3 cut(s) 187, 285, 301
Bst4CI ACNGT 2 cut(s) 119, 151
BstAPI GCANNNNNTGC 2 cut(s) 182, 383
BstC8I GCNNGC 1 cut(s) 388
BstDEI CTNAG 2 cut(s) 140, 604
BstF5I GGATG 3 cut(s) 473, 568, 655
BstKTI GATC 3 cut(s) 190, 288, 304
BstMAI GTCTC 1 cut(s) 399
BstMBI GATC 3 cut(s) 187, 285, 301
BstMWI GCNNNNNNNGC 3 cut(s) 182, 383, 489
BstSFI CTRYAG 1 cut(s) 432
BstSLI GKGCMC 1 cut(s) 471
BstV1I GCAGC 1 cut(s) 361
BstX2I RGATCY 1 cut(s) 187
BstYI RGATCY 1 cut(s) 187
BsuRI GGCC 2 cut(s) 77, 349
BtsCI GGATG 3 cut(s) 473, 568, 655
Cac8I GCNNGC 1 cut(s) 388
CaiI CAGNNNCTG 1 cut(s) 397
Cfr13I GGNCC 1 cut(s) 168
Csp6I GTAC 2 cut(s) 21, 437
CviAII CATG 3 cut(s) 9, 526, 610
CviJI RGCY 9 cut(s) 77, 129, 210, 224, 349, 374, 390, 407, 603
CviKI_1 RGCY 9 cut(s) 77, 129, 210, 224, 349, 374, 390, 407, 603
CviQI GTAC 2 cut(s) 21, 437
DdeI CTNAG 2 cut(s) 140, 604
DpnI GATC 3 cut(s) 189, 287, 303
DpnII GATC 3 cut(s) 187, 285, 301
Eco32I GATATC 1 cut(s) 402
Eco47I GGWCC 1 cut(s) 168
Eco57I CTGAAG 1 cut(s) 528
Eco88I CYCGRG 1 cut(s) 453
EcoRV GATATC 1 cut(s) 402
EcoT22I ATGCAT 1 cut(s) 586
FaeI CATG 3 cut(s) 12, 529, 613
FatI CATG 3 cut(s) 8, 525, 609
FauI CCCGC 2 cut(s) 33, 259
FauNDI CATATG 1 cut(s) 262
FbaI TGATCA 2 cut(s) 285, 301
Fnu4HI GCNGC 1 cut(s) 375
FokI GGATG 3 cut(s) 460, 575, 642
Fsp4HI GCNGC 1 cut(s) 375
FspBI CTAG 1 cut(s) 480
GluI GCNGC 1 cut(s) 375
HaeIII GGCC 2 cut(s) 77, 349
Hin1II CATG 3 cut(s) 12, 529, 613
HinfI GANTC 2 cut(s) 292, 315
HphI GGTGA 2 cut(s) 75, 610
Hpy166II GTNNAC 3 cut(s) 234, 469, 499
Hpy188I TCNGA 4 cut(s) 172, 219, 258, 399
Hpy188III TCNNGA 1 cut(s) 289
Hpy8I GTNNAC 3 cut(s) 234, 469, 499
HpyAV CCTTC 4 cut(s) 119, 255, 454, 482
HpyCH4III ACNGT 2 cut(s) 119, 151
HpyCH4IV ACGT 1 cut(s) 410
HpyCH4V TGCA 7 cut(s) 8, 159, 185, 377, 386, 469, 584
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 383, 489
HpyF3I CTNAG 2 cut(s) 140, 604
HpySE526I ACGT 1 cut(s) 410
Hsp92II CATG 3 cut(s) 12, 529, 613
Ksp22I TGATCA 2 cut(s) 285, 301
Kzo9I GATC 3 cut(s) 187, 285, 301
LmnI GCTCC 1 cut(s) 215
LpnPI CCDG 7 cut(s) 115, 150, 236, 296, 331, 404, 519
Lsp1109I GCAGC 1 cut(s) 361
LweI GCATC 2 cut(s) 54, 168
MaeI CTAG 1 cut(s) 480
MaeII ACGT 1 cut(s) 410
MalI GATC 3 cut(s) 189, 287, 303
MbiI CCGCTC 1 cut(s) 28
MboI GATC 3 cut(s) 187, 285, 301
MboII GAAGA 1 cut(s) 88
MflI RGATCY 1 cut(s) 187
MhlI GDGCHC 1 cut(s) 471
MluCI AATT 2 cut(s) 36, 514
MnlI CCTC 3 cut(s) 166, 462, 487
Mph1103I ATGCAT 1 cut(s) 586
MslI CAYNNNNRTG 2 cut(s) 309, 524
MwoI GCNNNNNNNGC 3 cut(s) 182, 383, 489
NdeI CATATG 1 cut(s) 262
NdeII GATC 3 cut(s) 187, 285, 301
NlaIII CATG 3 cut(s) 12, 529, 613
NlaIV GGNNCC 1 cut(s) 169
NsiI ATGCAT 1 cut(s) 586
PaeR7I CTCGAG 1 cut(s) 453
PfeI GAWTC 2 cut(s) 292, 315
PkrI GCNGC 1 cut(s) 376
PspN4I GGNNCC 1 cut(s) 169
PspPI GGNCC 1 cut(s) 168
PspXI VCTCGAGB 1 cut(s) 453
PstNI CAGNNNCTG 1 cut(s) 397
PsuI RGATCY 1 cut(s) 187
RsaI GTAC 2 cut(s) 22, 438
RsaNI GTAC 2 cut(s) 21, 437
RseI CAYNNNNRTG 2 cut(s) 309, 524
SatI GCNGC 1 cut(s) 375
Sau3AI GATC 3 cut(s) 187, 285, 301
Sau96I GGNCC 1 cut(s) 168
ScaI AGTACT 1 cut(s) 438
SduI GDGCHC 1 cut(s) 471
SetI ASST 9 cut(s) 212, 226, 285, 376, 413, 465, 498, 605, 668
SfaNI GCATC 2 cut(s) 54, 168
SfcI CTRYAG 1 cut(s) 432
Sfr274I CTCGAG 1 cut(s) 453
SinI GGWCC 1 cut(s) 168
SlaI CTCGAG 1 cut(s) 453
SmiMI CAYNNNNRTG 2 cut(s) 309, 524
SmlI CTYRAG 1 cut(s) 453
SmoI CTYRAG 1 cut(s) 453
Sse9I AATT 2 cut(s) 36, 514
SsiI CCGC 3 cut(s) 26, 266, 638
SspI AATATT 1 cut(s) 627
SspMI CTAG 1 cut(s) 480
TaaI ACNGT 2 cut(s) 119, 151
TaiI ACGT 1 cut(s) 413
TaqI TCGA 1 cut(s) 454
TasI AATT 2 cut(s) 36, 514
TatI WGTACW 1 cut(s) 436
TfiI GAWTC 2 cut(s) 292, 315
TseI GCWGC 1 cut(s) 374
TspGWI ACGGA 1 cut(s) 89
VneI GTGCAC 1 cut(s) 467
VpaK11BI GGWCC 1 cut(s) 168
XapI RAATTY 1 cut(s) 36
XhoI CTCGAG 1 cut(s) 453
XspI CTAG 1 cut(s) 480
ZrmI AGTACT 1 cut(s) 438
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.