RchiOBHm_Chr2g0152531

Polyamine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
N/A
Physical Location & Seq
Forward (+)
69929248 .. 69931217
1970 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 738 bp
ATGGCTGGAGGCAACAGTAACAGTAATAGTAATATTACTCCCCAATTGCGCAGAGCTGTTTGCAATTCCAATGTTGAGAGGAGGCCGACGTCGACACCGACGCCGTCTCCATCTGTGATAGTGATAGGTGGAGGCATGGCTGGTATTGCAGCTGCTCGTGCTCTCCAAGATGCATCATTCCAGGTTGTGCTGTTGGAGTCTCGGGATCGACTTGGTGGTCGAGTTCACACCGATTACTCGTTTGGTTTTCCGATTGACTTGGGTGCATCATGGTTGCATGGAGTATGTAAAGAGAATCCCTTGGCTCCATTAATTGGGAGACTGGGCTTGCCTTTATATCGTACCAGTGGTGATAACTCTGTGTTGTATGATCATGACTTAGAAAGCTATGCACTCTTTGATATGGATGGGAAACAAGTTCCTCAAGATTTGGTTACAAAAGTTGGTGTAAGATTTGAGGAAATTTTAAAAGAGACAGATGAGGTAAGAAAGGAACTCTCAGAAGACATGTCCATCTCCCGCGCTTTCTCAATTGTCTTTGAAAGGAAACCAGAATTAAGGTTGGAAGGAATTTCCCATAAGGTACTGCAGTGGTATTTGTGCCGAATGGAAGGCTGGTTTGCAGCAGATGCTGATACAATTTCACTTAAAAGCTGGGATCAGGCAAGCTTCTCATCTATTCTTGTCTGGTTAAGCAAAAGATGTCAAGCAATGATCCTTTGTTTTTTTATTTTTTAA

Protein Analysis

245

Amino Acids

27.26

Weight (kDa)

5.85

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_2 PF00890 39 - 82 4.7e-07 FAD binding domain
FAD_oxidored PF12831 39 - 74 5.8e-07 FAD dependent oxidoreductase
DAO PF01266 39 - 73 1e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 41 - 100 8.7e-15 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 46 - 214 1e-30 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 216
AatII GACGTC 1 cut(s) 92
Acc16I TGCGCA 1 cut(s) 50
AccB7I CCANNNNNTGG 1 cut(s) 314
AccI GTMKAC 1 cut(s) 92
AccII CGCG 1 cut(s) 522
AciI CCGC 1 cut(s) 520
AclWI GGATC 3 cut(s) 213, 666, 709
AcsI RAATTY 2 cut(s) 462, 570
AcyI GRCGYC 2 cut(s) 89, 101
AfaI GTAC 2 cut(s) 343, 585
AfiI CCNNNNNNNGG 1 cut(s) 314
AflIII ACRYGT 1 cut(s) 507
AgsI TTSAA 1 cut(s) 542
AjnI CCWGG 1 cut(s) 180
AluBI AGCT 5 cut(s) 56, 152, 387, 654, 669
AluI AGCT 5 cut(s) 56, 152, 387, 654, 669
Alw21I GWGCWC 1 cut(s) 163
Alw26I GTCTC 4 cut(s) 111, 204, 313, 467
AlwI GGATC 3 cut(s) 213, 666, 709
AlwNI CAGNNNCTG 1 cut(s) 632
Ama87I CYCGRG 1 cut(s) 201
AoxI GGCC 1 cut(s) 83
ApeKI GCWGC 3 cut(s) 149, 152, 623
ApoI RAATTY 2 cut(s) 462, 570
AseI ATTAAT 1 cut(s) 311
AspLEI GCGC 2 cut(s) 51, 524
AsuHPI GGTGA 1 cut(s) 362
AvaI CYCGRG 1 cut(s) 201
BaeI ACNNNNGTAYC 2 cut(s) 575, 608
BauI CACGAG 1 cut(s) 156
BbsI GAAGAC 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 163
BbvI GCAGC 3 cut(s) 139, 161, 635
BccI CCATC 3 cut(s) 118, 401, 521
BceAI ACGGC 1 cut(s) 88
BciT130I CCWGG 1 cut(s) 182
BclI TGATCA 1 cut(s) 370
BcoDI GTCTC 4 cut(s) 111, 204, 313, 467
BfmI CTRYAG 1 cut(s) 587
BisI GCNGC 3 cut(s) 150, 153, 624
BlsI GCNGC 3 cut(s) 151, 154, 625
Bme1390I CCNGG 1 cut(s) 182
BmeT110I CYCGRG 1 cut(s) 201
BmiI GGNNCC 1 cut(s) 306
BmrFI CCNGG 1 cut(s) 182
BmrI ACTGGG 1 cut(s) 332
BmsI GCATC 4 cut(s) 160, 182, 275, 619
BmuI ACTGGG 1 cut(s) 332
BpiI GAAGAC 1 cut(s) 510
BpmI CTGGAG 1 cut(s) 27
BpuEI CTTGAG 1 cut(s) 408
BsaHI GRCGYC 2 cut(s) 89, 101
BsaJI CCNNGG 1 cut(s) 300
BsaXI ACNNNNNCTCC 2 cut(s) 91, 121
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse1I ACTGG 2 cut(s) 327, 345
Bse3DI GCAATG 1 cut(s) 717
BseBI CCWGG 1 cut(s) 182
BseDI CCNNGG 1 cut(s) 300
BseGI GGATG 1 cut(s) 412
BseLI CCNNNNNNNGG 1 cut(s) 314
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 1 cut(s) 513
BseNI ACTGG 2 cut(s) 327, 345
BseRI GAGGAG 1 cut(s) 94
BseXI GCAGC 3 cut(s) 139, 161, 635
BseYI CCCAGC 1 cut(s) 654
Bsh1236I CGCG 1 cut(s) 522
BshFI GGCC 1 cut(s) 85
BsiHKAI GWGCWC 1 cut(s) 163
BsiHKCI CYCGRG 1 cut(s) 201
BslI CCNNNNNNNGG 1 cut(s) 314
BsmAI GTCTC 4 cut(s) 111, 204, 313, 467
BsmBI CGTCTC 1 cut(s) 111
BsnI GGCC 1 cut(s) 85
BsoBI CYCGRG 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 163
Bsp143I GATC 4 cut(s) 205, 370, 658, 714
BspACI CCGC 1 cut(s) 520
BspANI GGCC 1 cut(s) 85
BspCNI CTCAG 1 cut(s) 512
BspFNI CGCG 1 cut(s) 522
BspHI TCATGA 1 cut(s) 373
BspLI GGNNCC 1 cut(s) 306
BspMAI CTGCAG 1 cut(s) 591
BspPI GGATC 3 cut(s) 213, 666, 709
BsrDI GCAATG 1 cut(s) 717
BsrI ACTGG 2 cut(s) 327, 345
BssECI CCNNGG 1 cut(s) 300
BssMI GATC 4 cut(s) 205, 370, 658, 714
BssNI GRCGYC 2 cut(s) 89, 101
BssSI CACGAG 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 300
Bst2BI CACGAG 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 182
Bst4CI ACNGT 2 cut(s) 17, 23
BstACI GRCGYC 2 cut(s) 89, 101
BstAPI GCANNNNNTGC 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 329, 667
BstDEI CTNAG 2 cut(s) 379, 499
BstF5I GGATG 1 cut(s) 412
BstFNI CGCG 1 cut(s) 522
BstHHI GCGC 2 cut(s) 51, 524
BstKTI GATC 4 cut(s) 208, 373, 661, 717
BstMAI GTCTC 4 cut(s) 111, 204, 313, 467
BstMBI GATC 4 cut(s) 205, 370, 658, 714
BstMWI GCNNNNNNNGC 3 cut(s) 146, 158, 629
BstNI CCWGG 1 cut(s) 182
BstNSI RCATGY 1 cut(s) 511
BstSCI CCNGG 1 cut(s) 180
BstSFI CTRYAG 1 cut(s) 587
BstUI CGCG 1 cut(s) 522
BstV1I GCAGC 3 cut(s) 139, 161, 635
BstV2I GAAGAC 1 cut(s) 510
BsuRI GGCC 1 cut(s) 85
BtsCI GGATG 1 cut(s) 412
BtsI GCAGTG 1 cut(s) 596
BtsIMutI CAGTG 2 cut(s) 352, 596
Cac8I GCNNGC 2 cut(s) 329, 667
CaiI CAGNNNCTG 1 cut(s) 632
CciI TCATGA 1 cut(s) 373
CfoI GCGC 2 cut(s) 51, 524
CseI GACGC 1 cut(s) 109
Csp6I GTAC 2 cut(s) 342, 584
CviAII CATG 5 cut(s) 136, 270, 278, 374, 508
CviQI GTAC 2 cut(s) 342, 584
DdeI CTNAG 2 cut(s) 379, 499
DpnI GATC 4 cut(s) 207, 372, 660, 716
DpnII GATC 4 cut(s) 205, 370, 658, 714
DraI TTTAAA 1 cut(s) 468
DrdI GACNNNNNNGTC 1 cut(s) 216
DseDI GACNNNNNNGTC 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 300
Eco88I CYCGRG 1 cut(s) 201
EcoRII CCWGG 1 cut(s) 180
EcoT14I CCWWGG 1 cut(s) 300
EcoT22I ATGCAT 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 300
Esp3I CGTCTC 1 cut(s) 111
FaeI CATG 5 cut(s) 139, 273, 281, 377, 511
FatI CATG 5 cut(s) 135, 269, 277, 373, 507
FauI CCCGC 1 cut(s) 527
FbaI TGATCA 1 cut(s) 370
FblI GTMKAC 1 cut(s) 92
Fnu4HI GCNGC 3 cut(s) 150, 153, 624
FokI GGATG 1 cut(s) 419
Fsp4HI GCNGC 3 cut(s) 150, 153, 624
FspI TGCGCA 1 cut(s) 50
GlaI GCGC 2 cut(s) 50, 523
GluI GCNGC 3 cut(s) 150, 153, 624
GsaI CCCAGC 1 cut(s) 658
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 1 cut(s) 85
HgaI GACGC 1 cut(s) 109
HhaI GCGC 2 cut(s) 51, 524
Hin1I GRCGYC 2 cut(s) 89, 101
Hin1II CATG 5 cut(s) 139, 273, 281, 377, 511
Hin6I GCGC 2 cut(s) 49, 522
HinP1I GCGC 2 cut(s) 49, 522
HincII GTYRAC 1 cut(s) 93
HindII GTYRAC 1 cut(s) 93
HindIII AAGCTT 1 cut(s) 667
HinfI GANTC 2 cut(s) 197, 295
HphI GGTGA 1 cut(s) 362
Hpy166II GTNNAC 2 cut(s) 93, 226
Hpy188I TCNGA 2 cut(s) 252, 502
Hpy188III TCNNGA 3 cut(s) 203, 374, 425
Hpy8I GTNNAC 2 cut(s) 93, 226
Hpy99I CGWCG 3 cut(s) 91, 94, 103
HpyAV CCTTC 2 cut(s) 560, 605
HpyCH4III ACNGT 2 cut(s) 17, 23
HpyCH4IV ACGT 1 cut(s) 89
HpyCH4V TGCA 8 cut(s) 63, 149, 173, 266, 277, 392, 589, 623
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 158, 629
HpyF3I CTNAG 2 cut(s) 379, 499
HpySE526I ACGT 1 cut(s) 89
Hsp92I GRCGYC 2 cut(s) 89, 101
Hsp92II CATG 5 cut(s) 139, 273, 281, 377, 511
HspAI GCGC 2 cut(s) 49, 522
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 4 cut(s) 205, 370, 658, 714
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 3 cut(s) 139, 161, 635
LweI GCATC 4 cut(s) 160, 182, 275, 619
MaeII ACGT 1 cut(s) 89
MaeIII GTNAC 2 cut(s) 17, 433
MalI GATC 4 cut(s) 207, 372, 660, 716
MboI GATC 4 cut(s) 205, 370, 658, 714
MboII GAAGA 1 cut(s) 515
MfeI CAATTG 2 cut(s) 44, 531
MhlI GDGCHC 1 cut(s) 163
MluCI AATT 8 cut(s) 44, 64, 312, 462, 531, 554, 570, 639
MlyI GAGTC 1 cut(s) 206
MmeI TCCRAC 2 cut(s) 174, 543
MnlI CCTC 6 cut(s) 72, 75, 125, 432, 451, 475
Mph1103I ATGCAT 1 cut(s) 175
MseI TTAA 6 cut(s) 311, 467, 557, 648, 692, 736
MspA1I CMGCKG 1 cut(s) 152
MspR9I CCNGG 1 cut(s) 182
MunI CAATTG 2 cut(s) 44, 531
MvaI CCWGG 1 cut(s) 182
MvnI CGCG 1 cut(s) 522
MwoI GCNNNNNNNGC 3 cut(s) 146, 158, 629
NdeII GATC 4 cut(s) 205, 370, 658, 714
NlaIII CATG 5 cut(s) 139, 273, 281, 377, 511
NlaIV GGNNCC 1 cut(s) 306
NsbI TGCGCA 1 cut(s) 50
NsiI ATGCAT 1 cut(s) 175
NspI RCATGY 1 cut(s) 511
PagI TCATGA 1 cut(s) 373
PciI ACATGT 1 cut(s) 507
PcsI WCGNNNNNNNCGW 1 cut(s) 95
PfeI GAWTC 1 cut(s) 295
PflFI GACNNNGTC 1 cut(s) 103
PflMI CCANNNNNTGG 1 cut(s) 314
PkrI GCNGC 3 cut(s) 151, 154, 625
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
PscI ACATGT 1 cut(s) 507
PshBI ATTAAT 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 180
PspFI CCCAGC 1 cut(s) 654
PspGI CCWGG 1 cut(s) 180
PspN4I GGNNCC 1 cut(s) 306
PstI CTGCAG 1 cut(s) 591
PstNI CAGNNNCTG 1 cut(s) 632
PsyI GACNNNGTC 1 cut(s) 103
PvuII CAGCTG 1 cut(s) 152
RsaI GTAC 2 cut(s) 343, 585
RsaNI GTAC 2 cut(s) 342, 584
SalI GTCGAC 1 cut(s) 91
SaqAI TTAA 6 cut(s) 311, 467, 557, 648, 692, 736
SatI GCNGC 3 cut(s) 150, 153, 624
Sau3AI GATC 4 cut(s) 205, 370, 658, 714
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 1 cut(s) 182
SduI GDGCHC 1 cut(s) 163
SfaNI GCATC 4 cut(s) 160, 182, 275, 619
SfcI CTRYAG 1 cut(s) 587
SmlI CTYRAG 1 cut(s) 423
SmoI CTYRAG 1 cut(s) 423
Sse9I AATT 8 cut(s) 44, 64, 312, 462, 531, 554, 570, 639
SsiI CCGC 1 cut(s) 520
SspI AATATT 1 cut(s) 34
StyD4I CCNGG 1 cut(s) 180
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 2 cut(s) 17, 23
TaiI ACGT 1 cut(s) 92
TaqI TCGA 3 cut(s) 92, 208, 220
TasI AATT 8 cut(s) 44, 64, 312, 462, 531, 554, 570, 639
TfiI GAWTC 1 cut(s) 295
Tru1I TTAA 6 cut(s) 311, 467, 557, 648, 692, 736
Tru9I TTAA 6 cut(s) 311, 467, 557, 648, 692, 736
TscAI CASTG 2 cut(s) 352, 596
TseI GCWGC 3 cut(s) 149, 152, 623
TspRI CASTG 2 cut(s) 352, 596
Tth111I GACNNNGTC 1 cut(s) 103
Van91I CCANNNNNTGG 1 cut(s) 314
VspI ATTAAT 1 cut(s) 311
XapI RAATTY 2 cut(s) 462, 570
XceI RCATGY 1 cut(s) 511
XmiI GTMKAC 1 cut(s) 92
ZraI GACGTC 1 cut(s) 90
Zsp2I ATGCAT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.