RchiOBHm_Chr2g0167871

Trimethylguanosine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
N/A
Physical Location & Seq
Reverse (-)
82197234 .. 82198024
791 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 384 bp
ATGAAGACGAAGAAGTTTTCAAAGCAGCGAAGGGCTCGTCGCTCTAAAAGGTCCAAGCTTTCGCAACTGCCAACTCAAACCCATGAGACAAGGAGAGTAACTGAAGAAGGGGTGAGCCCTCTTGTTGAGAAGTACTGGTTTCAAAGATATGATCTTTTCTCGAGGTATGATGAGGGAATCAAGTTGGATGAACAGGGCTGGTACCCTGTCACTCCTGAACAAATTGCAATTAGACAAGCCAAGAGGTGTGAAAACGCAACTGTCGTTATTGATTGCTTTGCCGGCGTCGGAGGCAATGCCATTCAATTTGCATCACTCTTAGGTGTTGATTCACAGTTGCTTAACTACCAGTTCGACAAGTTCTTAGCTTTATGGTTTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Methyltransf_15

Protein Analysis

127

Amino Acids

14.86

Weight (kDa)

9.61

Isoelectric Point (pI)

58.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 201
AccB1I GGYRCC 1 cut(s) 201
AcuI CTGAAG 1 cut(s) 123
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 2 cut(s) 134, 203
AgsI TTSAA 3 cut(s) 21, 143, 305
AluBI AGCT 2 cut(s) 58, 368
AluI AGCT 2 cut(s) 58, 368
Alw26I GTCTC 1 cut(s) 80
Ama87I CYCGRG 1 cut(s) 160
ApeKI GCWGC 1 cut(s) 25
ArsI GACNNNNNNTTYG 2 cut(s) 22, 54
Asp718I GGTACC 1 cut(s) 201
AspS9I GGNCC 1 cut(s) 51
AsuHPI GGTGA 1 cut(s) 124
AvaI CYCGRG 1 cut(s) 160
AvaII GGWCC 1 cut(s) 51
BanI GGYRCC 1 cut(s) 201
BanII GRGCYC 2 cut(s) 37, 119
BbsI GAAGAC 1 cut(s) 11
BbvI GCAGC 1 cut(s) 37
BcoDI GTCTC 1 cut(s) 80
BisI GCNGC 1 cut(s) 26
BlsI GCNGC 1 cut(s) 27
BmcAI AGTACT 1 cut(s) 134
Bme18I GGWCC 1 cut(s) 51
BmeT110I CYCGRG 1 cut(s) 160
BmgT120I GGNCC 1 cut(s) 51
BmiI GGNNCC 1 cut(s) 203
BmsI GCATC 1 cut(s) 320
BpiI GAAGAC 1 cut(s) 11
BsaHI GRCGYC 1 cut(s) 285
Bse118I RCCGGY 1 cut(s) 281
Bse1I ACTGG 2 cut(s) 140, 349
Bse3DI GCAATG 1 cut(s) 301
BseGI GGATG 1 cut(s) 193
BseMI GCAATG 1 cut(s) 301
BseNI ACTGG 2 cut(s) 140, 349
BseXI GCAGC 1 cut(s) 37
BshNI GGYRCC 1 cut(s) 201
BsiHKCI CYCGRG 1 cut(s) 160
BsiSI CCGG 1 cut(s) 282
BsmAI GTCTC 1 cut(s) 80
BsoBI CYCGRG 1 cut(s) 160
Bsp1286I GDGCHC 2 cut(s) 37, 119
Bsp143I GATC 1 cut(s) 151
BspLI GGNNCC 1 cut(s) 203
BspT107I GGYRCC 1 cut(s) 201
BsrDI GCAATG 1 cut(s) 301
BsrFI RCCGGY 1 cut(s) 281
BsrI ACTGG 2 cut(s) 140, 349
BssAI RCCGGY 1 cut(s) 281
BssMI GATC 1 cut(s) 151
BssNI GRCGYC 1 cut(s) 285
Bst4CI ACNGT 2 cut(s) 262, 336
BstACI GRCGYC 1 cut(s) 285
BstC8I GCNNGC 1 cut(s) 283
BstDEI CTNAG 2 cut(s) 319, 364
BstF5I GGATG 1 cut(s) 193
BstKTI GATC 1 cut(s) 154
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 1 cut(s) 151
BstMWI GCNNNNNNNGC 2 cut(s) 282, 291
BstV1I GCAGC 1 cut(s) 37
BstV2I GAAGAC 1 cut(s) 11
BtsCI GGATG 1 cut(s) 193
Cac8I GCNNGC 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 281
Cfr13I GGNCC 1 cut(s) 51
CseI GACGC 1 cut(s) 274
Csp6I GTAC 2 cut(s) 133, 202
CviAII CATG 2 cut(s) 83, 381
CviJI RGCY 6 cut(s) 35, 58, 117, 198, 239, 368
CviKI_1 RGCY 6 cut(s) 35, 58, 117, 198, 239, 368
CviQI GTAC 2 cut(s) 133, 202
DdeI CTNAG 2 cut(s) 319, 364
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
Eco24I GRGCYC 2 cut(s) 37, 119
Eco47I GGWCC 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 160
EcoT38I GRGCYC 2 cut(s) 37, 119
FaeI CATG 2 cut(s) 86, 384
FaiI YATR 5 cut(s) 84, 150, 168, 373, 382
FatI CATG 2 cut(s) 82, 380
Fnu4HI GCNGC 1 cut(s) 26
FokI GGATG 1 cut(s) 200
FriOI GRGCYC 2 cut(s) 37, 119
Fsp4HI GCNGC 1 cut(s) 26
GluI GCNGC 1 cut(s) 26
HapII CCGG 1 cut(s) 282
HgaI GACGC 1 cut(s) 274
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 2 cut(s) 86, 384
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 2 cut(s) 177, 329
HpaII CCGG 1 cut(s) 282
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 1 cut(s) 378
Hpy188I TCNGA 1 cut(s) 290
Hpy188III TCNNGA 2 cut(s) 160, 215
Hpy8I GTNNAC 1 cut(s) 378
Hpy99I CGWCG 2 cut(s) 42, 290
HpyAV CCTTC 2 cut(s) 24, 101
HpyCH4III ACNGT 2 cut(s) 262, 336
HpyCH4V TGCA 2 cut(s) 227, 311
HpyF10VI GCNNNNNNNGC 2 cut(s) 282, 291
HpyF3I CTNAG 2 cut(s) 319, 364
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 2 cut(s) 86, 384
KpnI GGTACC 1 cut(s) 205
KroI GCCGGC 1 cut(s) 281
KroNI GCCGGC 1 cut(s) 283
Kzo9I GATC 1 cut(s) 151
LpnPI CCDG 7 cut(s) 121, 179, 184, 219, 228, 295, 362
Lsp1109I GCAGC 1 cut(s) 37
LweI GCATC 1 cut(s) 320
MaeIII GTNAC 2 cut(s) 97, 208
MalI GATC 1 cut(s) 153
MboI GATC 1 cut(s) 151
MboII GAAGA 3 cut(s) 16, 22, 116
MhlI GDGCHC 2 cut(s) 37, 119
MluCI AATT 3 cut(s) 222, 228, 305
MmeI TCCRAC 2 cut(s) 165, 268
MnlI CCTC 5 cut(s) 129, 156, 166, 237, 284
MroNI GCCGGC 1 cut(s) 281
MseI TTAA 1 cut(s) 342
MspI CCGG 1 cut(s) 282
MwoI GCNNNNNNNGC 2 cut(s) 282, 291
NaeI GCCGGC 1 cut(s) 283
NdeII GATC 1 cut(s) 151
NgoMIV GCCGGC 1 cut(s) 281
NlaIII CATG 2 cut(s) 86, 384
NlaIV GGNNCC 1 cut(s) 203
NmuCI GTSAC 1 cut(s) 208
PaeR7I CTCGAG 1 cut(s) 160
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PdiI GCCGGC 1 cut(s) 283
PfeI GAWTC 2 cut(s) 177, 329
PkrI GCNGC 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 203
PspPI GGNCC 1 cut(s) 51
RsaI GTAC 2 cut(s) 134, 203
RsaNI GTAC 2 cut(s) 133, 202
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 1 cut(s) 26
Sau3AI GATC 1 cut(s) 151
Sau96I GGNCC 1 cut(s) 51
ScaI AGTACT 1 cut(s) 134
SduI GDGCHC 2 cut(s) 37, 119
SetI ASST 6 cut(s) 53, 60, 167, 248, 325, 370
SfaNI GCATC 1 cut(s) 320
Sfr274I CTCGAG 1 cut(s) 160
SinI GGWCC 1 cut(s) 51
SlaI CTCGAG 1 cut(s) 160
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
Sse9I AATT 3 cut(s) 222, 228, 305
TaaI ACNGT 2 cut(s) 262, 336
TaqI TCGA 2 cut(s) 161, 354
TasI AATT 3 cut(s) 222, 228, 305
TatI WGTACW 1 cut(s) 132
TfiI GAWTC 2 cut(s) 177, 329
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 1 cut(s) 25
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 2 cut(s) 17, 204
VpaK11BI GGWCC 1 cut(s) 51
XhoI CTCGAG 1 cut(s) 160
ZrmI AGTACT 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.