RchiOBHm_Chr1g0317611

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
N/A
Physical Location & Seq
Reverse (-)
5140856 .. 5144337
3482 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1029 bp
ATGAGTTGTGGAGAAAGCAAGGTTGTGTGTGTGACAGGAGCATCTGGGTTCATAGCATCATGGCTGGTCAATCTCTTATTGCGACGAGGTTTTACTGTCAAAGCCACTGTCCGTGACCCAAATGATCAAAATAATACAGAACACCTACTCTTACTTGACGGAGCAAAAGATAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAAGGGTCTTTTGATACGGCAATTGATGGATGTCAATGTGTTTTCCATACGGCATCCCCTGTACTTCTTTCAGTCACTGATCCACAGTTATCGATACAAGTCGATTTTATCTGCAAATACTATAATCAAGACCCGCTAGATTGTTGTCAGGCAGAATTAATTGATCCTGCTTTGAAGGGAACACTTGATGTCCTCAGATCATGTGCAAATACTCCGTCTGTGAAAAGGGTGGTTTTAACATCCTCTATTGTATCAGTTATGTTCAACGGAAAACCTCTTTCTCCTGATGTTACAGTTGATGAAACTTGGTTTTCAGATCAAACTTTCTGTGAAAAAGCAAAGCTTTGGTATCCGGTCTCAAAGGTCTTAGCTGCTTGGAAGTTTGCTAAAGAAAACAGAATTGATATGTTACGATATATCCAGGAATTAATAGTGATTGGCCTTCACTTACGGCCTATGGTGAACACAAGCATGGAACCCCTTCTAAAACTCGTAAATGCTTATAGATGGATTGATGTTAGAGATGTTGCAAATGCACATATTCTAGTCTTCCAGAATCCCTCAGCTAGTGGGAGATATTGTTTAGTTGGAAAAGTAGCCCACTGTGCTGAGGCTGTGAAAATTTCGCATGATATCTTCCCCGCTCTCAATCTTCCTGGAAATCAGATAGAACACAAAATATTTGACTCAAGTTTTGAGAGTTCAGGCTGTGTCTCTTTCCGGGGCAGATGTGCAGATGACCAACCTTTGGCACCAACTTGCCAAGTATCGAGAGAAAAGGCACAGATTTTGGGTGTTAAGTTTACTCCTCTGGAGGTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

342

Amino Acids

37.97

Weight (kDa)

6.08

Isoelectric Point (pI)

28.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 87 3.4e-08 NmrA-like family
Epimerase PF01370 9 - 102 6.6e-09 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 89 6.4e-06 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 105 6.6e-09 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 87 1.7e-07 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 955
AccB7I CCANNNNNTGG 1 cut(s) 952
AccBSI CCGCTC 1 cut(s) 848
AciI CCGC 2 cut(s) 338, 846
AclWI GGATC 2 cut(s) 278, 362
AcsI RAATTY 1 cut(s) 825
AfaI GTAC 1 cut(s) 267
AfiI CCNNNNNNNGG 1 cut(s) 952
AgsI TTSAA 3 cut(s) 187, 379, 469
AjnI CCWGG 2 cut(s) 624, 859
AluBI AGCT 3 cut(s) 547, 575, 770
AluI AGCT 3 cut(s) 547, 575, 770
Alw26I GTCTC 2 cut(s) 565, 922
AlwI GGATC 2 cut(s) 278, 362
AlwNI CAGNNNCTG 1 cut(s) 281
AoxI GGCC 2 cut(s) 643, 656
ApeKI GCWGC 1 cut(s) 575
ApoI RAATTY 1 cut(s) 825
ArsI GACNNNNNNTTYG 6 cut(s) 93, 125, 404, 436, 881, 913
AseI ATTAAT 2 cut(s) 362, 632
Asp700I GAANNNNTTC 1 cut(s) 684
AsuC2I CCSGG 1 cut(s) 926
AsuHPI GGTGA 1 cut(s) 676
BanI GGYRCC 1 cut(s) 955
BbsI GAAGAC 2 cut(s) 745, 1015
BbvCI CCTCAGC 2 cut(s) 766, 813
BbvI GCAGC 1 cut(s) 562
BccI CCATC 2 cut(s) 224, 705
BceAI ACGGC 3 cut(s) 237, 270, 671
BciT130I CCWGG 2 cut(s) 626, 861
BciVI GTATCC 1 cut(s) 564
BclI TGATCA 1 cut(s) 124
BcnI CCSGG 1 cut(s) 926
BcoDI GTCTC 2 cut(s) 565, 922
BfaI CTAG 3 cut(s) 341, 749, 771
BfuI GTATCC 1 cut(s) 564
BisI GCNGC 1 cut(s) 576
BlsI GCNGC 1 cut(s) 577
Bme1390I CCNGG 3 cut(s) 626, 861, 926
BmiI GGNNCC 2 cut(s) 681, 957
BmrFI CCNGG 3 cut(s) 626, 861, 926
BmsI GCATC 3 cut(s) 50, 65, 266
BpiI GAAGAC 2 cut(s) 745, 1015
Bpu10I CCTNAGC 2 cut(s) 766, 813
BpuEI CTTGAG 1 cut(s) 877
BpuMI CCSGG 1 cut(s) 926
Bsa29I ATCGAT 1 cut(s) 296
BsaI GGTCTC 1 cut(s) 565
BsaJI CCNNGG 1 cut(s) 925
BsaWI WCCGGW 1 cut(s) 556
BsaXI ACNNNNNCTCC 2 cut(s) 769, 799
Bsc4I CCNNNNNNNGG 1 cut(s) 952
BseBI CCWGG 2 cut(s) 626, 861
BseCI ATCGAT 1 cut(s) 296
BseDI CCNNGG 1 cut(s) 925
BseGI GGATG 3 cut(s) 239, 257, 443
BseLI CCNNNNNNNGG 1 cut(s) 952
BseMII CTCAG 3 cut(s) 412, 780, 804
BseRI GAGGAG 1 cut(s) 1002
BseXI GCAGC 1 cut(s) 562
BsgI GTGCAG 1 cut(s) 957
BshFI GGCC 2 cut(s) 645, 658
BshNI GGYRCC 1 cut(s) 955
BshVI ATCGAT 1 cut(s) 296
BsiSI CCGG 2 cut(s) 557, 925
BslI CCNNNNNNNGG 1 cut(s) 952
BsmAI GTCTC 2 cut(s) 565, 922
BsnI GGCC 2 cut(s) 645, 658
Bso31I GGTCTC 1 cut(s) 565
Bsp143I GATC 5 cut(s) 124, 283, 367, 401, 520
BspACI CCGC 2 cut(s) 338, 846
BspANI GGCC 2 cut(s) 645, 658
BspCNI CTCAG 3 cut(s) 411, 779, 805
BspDI ATCGAT 1 cut(s) 296
BspLI GGNNCC 2 cut(s) 681, 957
BspPI GGATC 2 cut(s) 278, 362
BspT107I GGYRCC 1 cut(s) 955
BspTNI GGTCTC 1 cut(s) 565
BsrBI CCGCTC 1 cut(s) 848
BssECI CCNNGG 1 cut(s) 925
BssMI GATC 5 cut(s) 124, 283, 367, 401, 520
Bst2UI CCWGG 2 cut(s) 626, 861
Bst4CI ACNGT 5 cut(s) 97, 109, 291, 499, 809
BstDEI CTNAG 4 cut(s) 398, 571, 766, 813
BstF5I GGATG 3 cut(s) 239, 257, 443
BstKTI GATC 5 cut(s) 127, 286, 370, 404, 523
BstMAI GTCTC 2 cut(s) 565, 922
BstMBI GATC 5 cut(s) 124, 283, 367, 401, 520
BstMWI GCNNNNNNNGC 1 cut(s) 809
BstNI CCWGG 2 cut(s) 626, 861
BstSCI CCNGG 3 cut(s) 624, 859, 924
BstV1I GCAGC 1 cut(s) 562
BstV2I GAAGAC 2 cut(s) 745, 1015
Bsu15I ATCGAT 1 cut(s) 296
BsuI GTATCC 1 cut(s) 564
BsuRI GGCC 2 cut(s) 645, 658
BsuTUI ATCGAT 1 cut(s) 296
BtsCI GGATG 3 cut(s) 239, 257, 443
BtsIMutI CAGTG 3 cut(s) 105, 279, 805
CaiI CAGNNNCTG 1 cut(s) 281
ClaI ATCGAT 1 cut(s) 296
Csp6I GTAC 1 cut(s) 266
CviAII CATG 4 cut(s) 60, 405, 676, 833
CviQI GTAC 1 cut(s) 266
DdeI CTNAG 4 cut(s) 398, 571, 766, 813
DpnI GATC 5 cut(s) 126, 285, 369, 403, 522
DpnII GATC 5 cut(s) 124, 283, 367, 401, 520
Eco31I GGTCTC 1 cut(s) 565
Eco32I GATATC 1 cut(s) 838
EcoRII CCWGG 2 cut(s) 624, 859
EcoRV GATATC 1 cut(s) 838
FaeI CATG 4 cut(s) 63, 408, 679, 836
FalI AAGNNNNNCTT 4 cut(s) 159, 191, 531, 563
FatI CATG 4 cut(s) 59, 404, 675, 832
FauI CCCGC 2 cut(s) 345, 853
FbaI TGATCA 1 cut(s) 124
Fnu4HI GCNGC 1 cut(s) 576
FokI GGATG 3 cut(s) 244, 246, 430
Fsp4HI GCNGC 1 cut(s) 576
FspBI CTAG 3 cut(s) 341, 749, 771
GluI GCNGC 1 cut(s) 576
HaeIII GGCC 2 cut(s) 645, 658
HapII CCGG 2 cut(s) 557, 925
Hin1II CATG 4 cut(s) 63, 408, 679, 836
HindIII AAGCTT 1 cut(s) 545
HinfI GANTC 2 cut(s) 760, 890
HpaII CCGG 2 cut(s) 557, 925
HphI GGTGA 1 cut(s) 676
Hpy166II GTNNAC 2 cut(s) 667, 1008
Hpy188I TCNGA 4 cut(s) 401, 520, 870, 1028
Hpy188III TCNNGA 5 cut(s) 332, 488, 757, 975, 1016
Hpy8I GTNNAC 2 cut(s) 667, 1008
Hpy99I CGWCG 1 cut(s) 87
HpyAV CCTTC 4 cut(s) 200, 373, 656, 695
HpyCH4III ACNGT 5 cut(s) 97, 109, 291, 499, 809
HpyCH4V TGCA 5 cut(s) 318, 410, 734, 740, 938
HpyF10VI GCNNNNNNNGC 1 cut(s) 809
HpyF3I CTNAG 4 cut(s) 398, 571, 766, 813
Hsp92II CATG 4 cut(s) 63, 408, 679, 836
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 5 cut(s) 124, 283, 367, 401, 520
LmnI GCTCC 2 cut(s) 38, 161
Lsp1109I GCAGC 1 cut(s) 562
LweI GCATC 3 cut(s) 50, 65, 266
MaeI CTAG 3 cut(s) 341, 749, 771
MaeIII GTNAC 5 cut(s) 31, 113, 277, 493, 612
MalI GATC 5 cut(s) 126, 285, 369, 403, 522
MbiI CCGCTC 1 cut(s) 848
MboI GATC 5 cut(s) 124, 283, 367, 401, 520
MboII GAAGA 5 cut(s) 215, 745, 832, 848, 1015
MfeI CAATTG 1 cut(s) 225
MluCI AATT 6 cut(s) 225, 359, 363, 603, 629, 825
MlyI GAGTC 1 cut(s) 884
MmeI TCCRAC 1 cut(s) 772
MnlI CCTC 8 cut(s) 80, 407, 457, 489, 775, 808, 1012, 1023
MroXI GAANNNNTTC 1 cut(s) 684
MseI TTAA 4 cut(s) 362, 440, 632, 1002
MslI CAYNNNNRTG 1 cut(s) 674
MspI CCGG 2 cut(s) 557, 925
MspR9I CCNGG 3 cut(s) 626, 861, 926
MunI CAATTG 1 cut(s) 225
MvaI CCWGG 2 cut(s) 626, 861
MwoI GCNNNNNNNGC 1 cut(s) 809
NciI CCSGG 1 cut(s) 926
NdeII GATC 5 cut(s) 124, 283, 367, 401, 520
NlaIII CATG 4 cut(s) 63, 408, 679, 836
NlaIV GGNNCC 2 cut(s) 681, 957
NmuCI GTSAC 3 cut(s) 31, 113, 277
PdmI GAANNNNTTC 1 cut(s) 684
PfeI GAWTC 1 cut(s) 760
PflMI CCANNNNNTGG 1 cut(s) 952
PfoI TCCNGGA 2 cut(s) 624, 859
PkrI GCNGC 1 cut(s) 577
PleI GAGTC 1 cut(s) 884
PpsI GAGTC 1 cut(s) 884
PshBI ATTAAT 2 cut(s) 362, 632
Psp6I CCWGG 2 cut(s) 624, 859
PspGI CCWGG 2 cut(s) 624, 859
PspN4I GGNNCC 2 cut(s) 681, 957
PstNI CAGNNNCTG 1 cut(s) 281
RsaI GTAC 1 cut(s) 267
RsaNI GTAC 1 cut(s) 266
RseI CAYNNNNRTG 1 cut(s) 674
SaqAI TTAA 4 cut(s) 362, 440, 632, 1002
SatI GCNGC 1 cut(s) 576
Sau3AI GATC 5 cut(s) 124, 283, 367, 401, 520
SchI GAGTC 1 cut(s) 884
ScrFI CCNGG 3 cut(s) 626, 861, 926
SfaNI GCATC 3 cut(s) 50, 65, 266
SmiMI CAYNNNNRTG 1 cut(s) 674
SmlI CTYRAG 1 cut(s) 892
SmoI CTYRAG 1 cut(s) 892
Sse9I AATT 6 cut(s) 225, 359, 363, 603, 629, 825
SsiI CCGC 2 cut(s) 338, 846
SspI AATATT 1 cut(s) 885
SspMI CTAG 3 cut(s) 341, 749, 771
StyD4I CCNGG 3 cut(s) 624, 859, 924
TaaI ACNGT 5 cut(s) 97, 109, 291, 499, 809
TaqI TCGA 3 cut(s) 296, 306, 974
TasI AATT 6 cut(s) 225, 359, 363, 603, 629, 825
TatI WGTACW 1 cut(s) 265
TfiI GAWTC 1 cut(s) 760
Tru1I TTAA 4 cut(s) 362, 440, 632, 1002
Tru9I TTAA 4 cut(s) 362, 440, 632, 1002
TscAI CASTG 3 cut(s) 112, 286, 812
TseFI GTSAC 3 cut(s) 31, 113, 277
TseI GCWGC 1 cut(s) 575
Tsp45I GTSAC 3 cut(s) 31, 113, 277
TspDTI ATGAA 3 cut(s) 40, 167, 519
TspGWI ACGGA 4 cut(s) 101, 174, 408, 486
TspRI CASTG 3 cut(s) 112, 286, 812
Van91I CCANNNNNTGG 1 cut(s) 952
VspI ATTAAT 2 cut(s) 362, 632
XapI RAATTY 1 cut(s) 825
XmnI GAANNNNTTC 1 cut(s) 684
XspI CTAG 3 cut(s) 341, 749, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.