RchiOBHm_Chr1g0324361

Ribosomal RNA large subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
N/A
Physical Location & Seq
Forward (+)
12038201 .. 12040068
1868 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 387 bp
ATGGAGCCTCCCCAGTCACTAAACCTTAGCTCCGGCAAGCCTCCAAGGAGAAAAGTGCCTCCGCCGTCAGATCTAACTAGACCGCAAAGGTGTGTTGCGAGGGTTGTACTCAAAAAGGGGAAGACACAATTATTCAAGGATGGAAGTCCAATGGTTCATAGTGGAGCAGTTGATAGAATAATTGGGAGACCACCACCGAGAACTGGAGACGTTGTGCTAGTAGCTGATGGAGCAGAAAAGCCAATAGGGTGGGGCATGTATAATTCAGTCTCCATGTTCTGTGTTCGGCTTATGCAACTGGATGAGGAAGCAACAAGGTACTATTTATATATGCAGTTCGAAATTATCACATTTGCAGTGATGTTGGAGTTGCTAATCTTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrans_SAM

Protein Analysis

128

Amino Acids

14.39

Weight (kDa)

9.47

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUA_3 PF17785 35 - 98 1.7e-10 PUA-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 62, 83
AfaI GTAC 2 cut(s) 108, 320
AfiI CCNNNNNNNGG 1 cut(s) 203
AgsI TTSAA 1 cut(s) 136
AluBI AGCT 2 cut(s) 30, 224
AluI AGCT 2 cut(s) 30, 224
Alw26I GTCTC 3 cut(s) 181, 201, 274
AsuII TTCGAA 1 cut(s) 339
BbsI GAAGAC 1 cut(s) 128
BccI CCATC 2 cut(s) 134, 221
BceAI ACGGC 1 cut(s) 49
BcoDI GTCTC 3 cut(s) 181, 201, 274
BfaI CTAG 2 cut(s) 78, 218
BglII AGATCT 1 cut(s) 70
BmiI GGNNCC 1 cut(s) 6
BmrI ACTGGG 1 cut(s) 7
BmuI ACTGGG 1 cut(s) 7
BpiI GAAGAC 1 cut(s) 128
BpmI CTGGAG 1 cut(s) 225
Bpu10I CCTNAGC 1 cut(s) 26
Bpu14I TTCGAA 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 181
BsaJI CCNNGG 1 cut(s) 44
BsaXI ACNNNNNCTCC 4 cut(s) 14, 44, 198, 228
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse1I ACTGG 3 cut(s) 13, 208, 303
BseDI CCNNGG 1 cut(s) 44
BseGI GGATG 2 cut(s) 145, 307
BseLI CCNNNNNNNGG 1 cut(s) 203
BseNI ACTGG 3 cut(s) 13, 208, 303
BsiSI CCGG 1 cut(s) 33
BslI CCNNNNNNNGG 1 cut(s) 203
BsmAI GTCTC 3 cut(s) 181, 201, 274
BsmBI CGTCTC 1 cut(s) 201
Bso31I GGTCTC 1 cut(s) 181
Bsp119I TTCGAA 1 cut(s) 339
Bsp143I GATC 1 cut(s) 70
BspACI CCGC 2 cut(s) 62, 83
BspLI GGNNCC 1 cut(s) 6
BspT104I TTCGAA 1 cut(s) 339
BspTNI GGTCTC 1 cut(s) 181
BsrI ACTGG 3 cut(s) 13, 208, 303
BssECI CCNNGG 1 cut(s) 44
BssMI GATC 1 cut(s) 70
BssT1I CCWWGG 1 cut(s) 44
BstBI TTCGAA 1 cut(s) 339
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 1 cut(s) 26
BstF5I GGATG 2 cut(s) 145, 307
BstKTI GATC 1 cut(s) 73
BstMAI GTCTC 3 cut(s) 181, 201, 274
BstMBI GATC 1 cut(s) 70
BstMWI GCNNNNNNNGC 1 cut(s) 230
BstNSI RCATGY 1 cut(s) 259
BstV2I GAAGAC 1 cut(s) 128
BstX2I RGATCY 1 cut(s) 70
BstXI CCANNNNNNTGG 1 cut(s) 249
BstYI RGATCY 1 cut(s) 70
BtsCI GGATG 2 cut(s) 145, 307
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 1 cut(s) 363
Cac8I GCNNGC 1 cut(s) 38
Csp6I GTAC 2 cut(s) 107, 319
CviAII CATG 2 cut(s) 256, 274
CviJI RGCY 6 cut(s) 7, 30, 40, 224, 241, 289
CviKI_1 RGCY 6 cut(s) 7, 30, 40, 224, 241, 289
CviQI GTAC 2 cut(s) 107, 319
DdeI CTNAG 1 cut(s) 26
DpnI GATC 1 cut(s) 72
DpnII GATC 1 cut(s) 70
EciI GGCGGA 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 44
Eco31I GGTCTC 1 cut(s) 181
EcoT14I CCWWGG 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 44
Esp3I CGTCTC 1 cut(s) 201
FaeI CATG 2 cut(s) 259, 277
FaiI YATR 8 cut(s) 159, 257, 261, 275, 293, 328, 330, 332
FatI CATG 2 cut(s) 255, 273
FokI GGATG 2 cut(s) 152, 314
FspBI CTAG 2 cut(s) 78, 218
GsuI CTGGAG 1 cut(s) 225
HapII CCGG 1 cut(s) 33
Hin1II CATG 2 cut(s) 259, 277
HpaII CCGG 1 cut(s) 33
Hpy188I TCNGA 1 cut(s) 70
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 3 cut(s) 295, 334, 356
HpyF10VI GCNNNNNNNGC 1 cut(s) 230
HpyF3I CTNAG 1 cut(s) 26
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 2 cut(s) 259, 277
Kzo9I GATC 1 cut(s) 70
LmnI GCTCC 4 cut(s) 4, 35, 164, 230
LpnPI CCDG 4 cut(s) 26, 46, 189, 284
MaeI CTAG 2 cut(s) 78, 218
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 1 cut(s) 15
MalI GATC 1 cut(s) 72
MboI GATC 1 cut(s) 70
MboII GAAGA 1 cut(s) 133
MflI RGATCY 1 cut(s) 70
MluCI AATT 4 cut(s) 128, 180, 262, 342
MmeI TCCRAC 1 cut(s) 345
MnlI CCTC 5 cut(s) 18, 51, 69, 93, 298
MspI CCGG 1 cut(s) 33
MwoI GCNNNNNNNGC 1 cut(s) 230
NdeII GATC 1 cut(s) 70
NlaIII CATG 2 cut(s) 259, 277
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 15
NspI RCATGY 1 cut(s) 259
NspV TTCGAA 1 cut(s) 339
PspN4I GGNNCC 1 cut(s) 6
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 2 cut(s) 108, 320
RsaNI GTAC 2 cut(s) 107, 319
Sau3AI GATC 1 cut(s) 70
SetI ASST 6 cut(s) 27, 32, 92, 213, 226, 320
SfuI TTCGAA 1 cut(s) 339
Sse9I AATT 4 cut(s) 128, 180, 262, 342
SsiI CCGC 2 cut(s) 62, 83
SspMI CTAG 2 cut(s) 78, 218
StyI CCWWGG 1 cut(s) 44
TaiI ACGT 1 cut(s) 213
TaqI TCGA 1 cut(s) 339
TasI AATT 4 cut(s) 128, 180, 262, 342
TatI WGTACW 1 cut(s) 106
TscAI CASTG 1 cut(s) 363
TseFI GTSAC 1 cut(s) 15
Tsp45I GTSAC 1 cut(s) 15
TspDTI ATGAA 1 cut(s) 146
TspRI CASTG 1 cut(s) 363
XceI RCATGY 1 cut(s) 259
XspI CTAG 2 cut(s) 78, 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.