RchiOBHm_Chr1g0329821

Component of the EKC KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
N/A
Physical Location & Seq
Reverse (-)
19578307 .. 19581552
3246 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1080 bp
ATGGTGACATCTCCCAGCTTGTCACCATGGATTCGCATCTTGTTTGCAATAGCCATGACTCTTGAGAATGAAGGGAGGTATCGAATCTTTGGAGAGACCATTGATATTGTTGTTGGAAATTGCTTGGATCGGTTTGCTAGGGCATTAGAACTCTCCAATGATCCAGCCCCCGGATATAACATTGAGCAAGTAGTCGGATCTGTCTGCAGCGTGGCAAGGAGGAGCGGTGGCGTGGGGTTGCGACGGCGTGGCATGGTGATGCGTGGCAGCGGAGTGGTGACATCGGAGCTCGCCGGCGTCGGGAAGGATGGTCTGGTCTGTTGGGGAAAGGGGTGTTTGGGCTGGGCTTCTTTTCTTGGGCCTCCCTGGTTTTGTTTTGTTTTTAGTTTCTTTAGGTTTTGGTGTGTCTGTCCGATTGGTGTTCTGTTGAAAATAAGTCTCTCCTCTTGTGAGCGAGGGTTAGGGTATTTCTGGATAGTGTCGATGCCATTGTTTTGGTGTCATTGTCGGTCTGCTGTGTCTTGGTCTGGTCTTTGGGTAGTGGTTTTTGGTTATCAATGTGATGGTGAATCACCCTTACATGGTCTGGCGGTTTTGGGTTGCGGTGTCCGAACGGCTTGCAAAGAAAGGAGAACAGTTTATAGACCTTCCTTATGCTGTCAAAGACTCAAAGGGATGGATGTGTCTTTTAGTGGAATTCTGAGCTACATTGAAGCCACTGCTGTGGAGAAACTAAAAAATGACGAGTGCACCCCTGCAGACCTGTGCCACTCACTTCAGAACGTGCAATGTGACAAGAGAGATGTACTTATTGTTGGTGGTGTGGGTTGCAATGAGCGGCTGCAAGAGATGATGAAAATCATGTGTGCTGAACGGGGTGGAAGATTGTTTGCAACTGATGATAGGTATTATATTGATAATGGGGCAATGATTGCTTATAATGGTCTTCTTGCTTTTGCTCACGGAACATCAACCCCATTGGAGGAATCGACTTTCACGCAAGGGTTCCGGACTGATGAAGTGGAAGCAATCTGGAGAGTAAAAGAGGAGTCGGCAAAAGTTAAAAGGGTATGGGAGTAG

Protein Analysis

359

Amino Acids

39.82

Weight (kDa)

8.02

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TsaD PF00814 222 - 313 1.3e-12 tRNA N6-adenosine threonylcarbamoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 939
AccBSI CCGCTC 2 cut(s) 225, 838
AccIII TCCGGA 1 cut(s) 1008
AciI CCGC 5 cut(s) 225, 270, 590, 603, 838
AclWI GGATC 3 cut(s) 135, 155, 205
AcsI RAATTY 1 cut(s) 696
AcuI CTGAAG 1 cut(s) 761
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 1 cut(s) 807
AfiI CCNNNNNNNGG 4 cut(s) 170, 300, 581, 982
AgsI TTSAA 2 cut(s) 430, 713
AjnI CCWGG 1 cut(s) 365
AleI CACNNNNGTG 1 cut(s) 722
AluBI AGCT 3 cut(s) 18, 289, 705
AluI AGCT 3 cut(s) 18, 289, 705
Alw21I GWGCWC 2 cut(s) 291, 752
Alw26I GTCTC 2 cut(s) 89, 443
Alw44I GTGCAC 1 cut(s) 748
AlwI GGATC 3 cut(s) 135, 155, 205
Aor13HI TCCGGA 1 cut(s) 1008
AoxI GGCC 1 cut(s) 359
ApaLI GTGCAC 1 cut(s) 748
ApeKI GCWGC 3 cut(s) 207, 267, 841
ApoI RAATTY 1 cut(s) 696
AspS9I GGNCC 1 cut(s) 359
AsuC2I CCSGG 1 cut(s) 171
AsuHPI GGTGA 6 cut(s) 15, 16, 268, 289, 564, 578
BaeGI GKGCMC 1 cut(s) 752
BanII GRGCYC 1 cut(s) 291
BbsI GAAGAC 1 cut(s) 938
Bbv12I GWGCWC 2 cut(s) 291, 752
BbvI GCAGC 3 cut(s) 219, 279, 828
BccI CCATC 3 cut(s) 302, 557, 670
BceAI ACGGC 2 cut(s) 260, 630
BcgI CGANNNNNNTGC 2 cut(s) 600, 634
BciT130I CCWGG 1 cut(s) 367
BcnI CCSGG 1 cut(s) 171
BcoDI GTCTC 2 cut(s) 89, 443
BfaI CTAG 1 cut(s) 138
BfmI CTRYAG 2 cut(s) 205, 756
BisI GCNGC 4 cut(s) 208, 268, 839, 842
BlsI GCNGC 4 cut(s) 209, 269, 840, 843
Bme1390I CCNGG 2 cut(s) 171, 367
BmgT120I GGNCC 1 cut(s) 359
BmiI GGNNCC 1 cut(s) 1007
BmrFI CCNGG 2 cut(s) 171, 367
BmsI GCATC 3 cut(s) 45, 249, 474
BpiI GAAGAC 1 cut(s) 938
BpmI CTGGAG 1 cut(s) 1054
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 171
BsaBI GATNNNNATC 2 cut(s) 35, 857
BsaHI GRCGYC 1 cut(s) 297
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 3 cut(s) 26, 169, 365
BsaWI WCCGGW 1 cut(s) 1008
BsaXI ACNNNNNCTCC 2 cut(s) 211, 241
Bsc4I CCNNNNNNNGG 4 cut(s) 170, 300, 581, 982
Bse118I RCCGGY 1 cut(s) 293
Bse3DI GCAATG 3 cut(s) 794, 838, 933
Bse8I GATNNNNATC 2 cut(s) 35, 857
BseAI TCCGGA 1 cut(s) 1008
BseBI CCWGG 1 cut(s) 367
BseDI CCNNGG 3 cut(s) 26, 169, 365
BseGI GGATG 3 cut(s) 313, 681, 685
BseJI GATNNNNATC 2 cut(s) 35, 857
BseLI CCNNNNNNNGG 4 cut(s) 170, 300, 581, 982
BseMI GCAATG 3 cut(s) 794, 838, 933
BseMII CTCAG 1 cut(s) 692
BseRI GAGGAG 3 cut(s) 235, 433, 1061
BseSI GKGCMC 1 cut(s) 752
BseXI GCAGC 3 cut(s) 219, 279, 828
BseYI CCCAGC 2 cut(s) 14, 342
BshFI GGCC 1 cut(s) 361
BsiHKAI GWGCWC 2 cut(s) 291, 752
BsiSI CCGG 3 cut(s) 171, 294, 1009
BslI CCNNNNNNNGG 4 cut(s) 170, 300, 581, 982
BsmAI GTCTC 2 cut(s) 89, 443
BsnI GGCC 1 cut(s) 361
Bso31I GGTCTC 1 cut(s) 89
Bsp1286I GDGCHC 2 cut(s) 291, 752
Bsp13I TCCGGA 1 cut(s) 1008
Bsp143I GATC 3 cut(s) 127, 160, 197
Bsp19I CCATGG 1 cut(s) 26
BspACI CCGC 5 cut(s) 225, 270, 590, 603, 838
BspANI GGCC 1 cut(s) 361
BspCNI CTCAG 1 cut(s) 693
BspEI TCCGGA 1 cut(s) 1008
BspLI GGNNCC 1 cut(s) 1007
BspMAI CTGCAG 2 cut(s) 209, 760
BspPI GGATC 3 cut(s) 135, 155, 205
BspTNI GGTCTC 1 cut(s) 89
BsrBI CCGCTC 2 cut(s) 225, 838
BsrDI GCAATG 3 cut(s) 794, 838, 933
BsrFI RCCGGY 1 cut(s) 293
BssAI RCCGGY 1 cut(s) 293
BssECI CCNNGG 3 cut(s) 26, 169, 365
BssMI GATC 3 cut(s) 127, 160, 197
BssNI GRCGYC 1 cut(s) 297
BssT1I CCWWGG 1 cut(s) 26
Bst2UI CCWGG 1 cut(s) 367
Bst4CI ACNGT 1 cut(s) 637
BstACI GRCGYC 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 932
BstC8I GCNNGC 3 cut(s) 291, 295, 619
BstDEI CTNAG 1 cut(s) 701
BstDSI CCRYGG 1 cut(s) 26
BstF5I GGATG 3 cut(s) 313, 681, 685
BstKTI GATC 3 cut(s) 130, 163, 200
BstMAI GTCTC 2 cut(s) 89, 443
BstMBI GATC 3 cut(s) 127, 160, 197
BstMWI GCNNNNNNNGC 1 cut(s) 932
BstNI CCWGG 1 cut(s) 367
BstSCI CCNGG 2 cut(s) 169, 365
BstSFI CTRYAG 2 cut(s) 205, 756
BstSLI GKGCMC 1 cut(s) 752
BstV1I GCAGC 3 cut(s) 219, 279, 828
BstV2I GAAGAC 1 cut(s) 938
BstX2I RGATCY 1 cut(s) 197
BstXI CCANNNNNNTGG 2 cut(s) 495, 724
BstYI RGATCY 1 cut(s) 197
BsuRI GGCC 1 cut(s) 361
BtgI CCRYGG 1 cut(s) 26
BtsCI GGATG 3 cut(s) 313, 681, 685
BtsI GCAGTG 1 cut(s) 717
BtsIMutI CAGTG 1 cut(s) 717
Cac8I GCNNGC 3 cut(s) 291, 295, 619
Cfr10I RCCGGY 1 cut(s) 293
Cfr13I GGNCC 1 cut(s) 359
CseI GACGC 1 cut(s) 286
Csp6I GTAC 1 cut(s) 806
CviAII CATG 5 cut(s) 27, 55, 253, 581, 862
CviQI GTAC 1 cut(s) 806
DdeI CTNAG 1 cut(s) 701
DpnI GATC 3 cut(s) 129, 162, 199
DpnII GATC 3 cut(s) 127, 160, 197
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 1 cut(s) 26
Eco24I GRGCYC 1 cut(s) 291
Eco31I GGTCTC 1 cut(s) 89
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 1 cut(s) 761
EcoICRI GAGCTC 1 cut(s) 289
EcoRI GAATTC 1 cut(s) 696
EcoRII CCWGG 1 cut(s) 365
EcoT14I CCWWGG 1 cut(s) 26
EcoT38I GRGCYC 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 26
FaeI CATG 5 cut(s) 30, 58, 256, 584, 865
FatI CATG 5 cut(s) 26, 54, 252, 580, 861
Fnu4HI GCNGC 4 cut(s) 208, 268, 839, 842
FokI GGATG 3 cut(s) 320, 688, 692
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 4 cut(s) 208, 268, 839, 842
FspBI CTAG 1 cut(s) 138
GluI GCNGC 4 cut(s) 208, 268, 839, 842
GsaI CCCAGC 2 cut(s) 18, 346
GsuI CTGGAG 1 cut(s) 1054
HaeIII GGCC 1 cut(s) 361
HapII CCGG 3 cut(s) 171, 294, 1009
HgaI GACGC 1 cut(s) 286
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 5 cut(s) 30, 58, 256, 584, 865
HinfI GANTC 7 cut(s) 31, 58, 84, 569, 666, 986, 1049
HpaII CCGG 3 cut(s) 171, 294, 1009
HphI GGTGA 6 cut(s) 15, 16, 268, 289, 564, 578
Hpy166II GTNNAC 1 cut(s) 750
Hpy188I TCNGA 6 cut(s) 197, 286, 414, 611, 702, 780
Hpy188III TCNNGA 5 cut(s) 62, 301, 472, 1009, 1033
Hpy8I GTNNAC 1 cut(s) 750
Hpy99I CGWCG 2 cut(s) 246, 302
HpyAV CCTTC 3 cut(s) 65, 298, 657
HpyCH4III ACNGT 1 cut(s) 637
HpyCH4IV ACGT 1 cut(s) 783
HpyCH4V TGCA 9 cut(s) 47, 207, 621, 750, 758, 787, 831, 844, 893
HpyF10VI GCNNNNNNNGC 1 cut(s) 932
HpyF3I CTNAG 1 cut(s) 701
HpySE526I ACGT 1 cut(s) 783
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 5 cut(s) 30, 58, 256, 584, 865
Kpn2I TCCGGA 1 cut(s) 1008
KroI GCCGGC 1 cut(s) 293
KroNI GCCGGC 1 cut(s) 295
Kzo9I GATC 3 cut(s) 127, 160, 197
LmnI GCTCC 2 cut(s) 222, 286
Lsp1109I GCAGC 3 cut(s) 219, 279, 828
LweI GCATC 3 cut(s) 45, 249, 474
MaeI CTAG 1 cut(s) 138
MaeII ACGT 1 cut(s) 783
MaeIII GTNAC 4 cut(s) 4, 21, 277, 791
MalI GATC 3 cut(s) 129, 162, 199
MbiI CCGCTC 2 cut(s) 225, 838
MboI GATC 3 cut(s) 127, 160, 197
MboII GAAGA 2 cut(s) 894, 938
MflI RGATCY 1 cut(s) 197
MhlI GDGCHC 2 cut(s) 291, 752
MluCI AATT 2 cut(s) 118, 696
MlyI GAGTC 3 cut(s) 52, 660, 1058
MmeI TCCRAC 2 cut(s) 94, 175
MnlI CCTC 7 cut(s) 69, 213, 372, 449, 454, 976, 1039
MreI CGCCGGCG 1 cut(s) 293
MroI TCCGGA 1 cut(s) 1008
MroNI GCCGGC 1 cut(s) 293
MseI TTAA 1 cut(s) 1062
MslI CAYNNNNRTG 2 cut(s) 257, 722
MspA1I CMGCKG 1 cut(s) 270
MspI CCGG 3 cut(s) 171, 294, 1009
MspR9I CCNGG 2 cut(s) 171, 367
MvaI CCWGG 1 cut(s) 367
MwoI GCNNNNNNNGC 1 cut(s) 932
NaeI GCCGGC 1 cut(s) 295
NciI CCSGG 1 cut(s) 171
NcoI CCATGG 1 cut(s) 26
NdeII GATC 3 cut(s) 127, 160, 197
NgoMIV GCCGGC 1 cut(s) 293
NlaIII CATG 5 cut(s) 30, 58, 256, 584, 865
NlaIV GGNNCC 1 cut(s) 1007
NmuCI GTSAC 4 cut(s) 4, 21, 277, 791
OliI CACNNNNGTG 1 cut(s) 722
PdiI GCCGGC 1 cut(s) 295
PfeI GAWTC 4 cut(s) 31, 84, 569, 986
PkrI GCNGC 4 cut(s) 209, 269, 840, 843
PleI GAGTC 3 cut(s) 52, 660, 1057
PpsI GAGTC 3 cut(s) 52, 660, 1057
PsiI TTATAA 1 cut(s) 939
Psp124BI GAGCTC 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 365
PspFI CCCAGC 2 cut(s) 14, 342
PspGI CCWGG 1 cut(s) 365
PspN4I GGNNCC 1 cut(s) 1007
PspPI GGNCC 1 cut(s) 359
PstI CTGCAG 2 cut(s) 209, 760
PsuI RGATCY 1 cut(s) 197
RsaI GTAC 1 cut(s) 807
RsaNI GTAC 1 cut(s) 806
RseI CAYNNNNRTG 2 cut(s) 257, 722
SacI GAGCTC 1 cut(s) 291
SaqAI TTAA 1 cut(s) 1062
SatI GCNGC 4 cut(s) 208, 268, 839, 842
Sau3AI GATC 3 cut(s) 127, 160, 197
Sau96I GGNCC 1 cut(s) 359
SchI GAGTC 3 cut(s) 52, 660, 1058
ScrFI CCNGG 2 cut(s) 171, 367
SduI GDGCHC 2 cut(s) 291, 752
SetI ASST 9 cut(s) 20, 80, 291, 398, 649, 707, 765, 786, 908
SfaNI GCATC 3 cut(s) 45, 249, 474
SfcI CTRYAG 2 cut(s) 205, 756
SgrAI CRCCGGYG 1 cut(s) 293
SmiMI CAYNNNNRTG 2 cut(s) 257, 722
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 2 cut(s) 118, 696
SsiI CCGC 5 cut(s) 225, 270, 590, 603, 838
SspMI CTAG 1 cut(s) 138
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 2 cut(s) 169, 365
StyI CCWWGG 1 cut(s) 26
TaaI ACNGT 1 cut(s) 637
TaiI ACGT 1 cut(s) 786
TaqI TCGA 3 cut(s) 82, 482, 989
TaqII GACCGA 1 cut(s) 498
TasI AATT 2 cut(s) 118, 696
TatI WGTACW 1 cut(s) 805
TauI GCSGC 1 cut(s) 841
TfiI GAWTC 4 cut(s) 31, 84, 569, 986
Tru1I TTAA 1 cut(s) 1062
Tru9I TTAA 1 cut(s) 1062
TscAI CASTG 1 cut(s) 724
TseFI GTSAC 4 cut(s) 4, 21, 277, 791
TseI GCWGC 3 cut(s) 207, 267, 841
Tsp45I GTSAC 4 cut(s) 4, 21, 277, 791
TspDTI ATGAA 3 cut(s) 84, 869, 1032
TspGWI ACGGA 1 cut(s) 978
TspRI CASTG 1 cut(s) 724
VneI GTGCAC 1 cut(s) 748
XapI RAATTY 1 cut(s) 696
XspI CTAG 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.